LOCUS       NZ_CP145706             7135 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-CAM-02-A plasmid p3-S1-CAM-02-A, complete
            sequence.
ACCESSION   NZ_CP145706
VERSION     NZ_CP145706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052293
            Assembly: GCF_036884785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204.7788X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884785.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:15:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,477
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,032
            CDSs (with protein)               :: 5,032
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 114 of 320
            Pseudo Genes (incomplete)         :: 216 of 320
            Pseudo Genes (internal stop)      :: 61 of 320
            Pseudo Genes (multiple problems)  :: 69 of 320
            Pseudo Genes (short protein)      :: 4 of 320
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-CAM-02-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3-S1-CAM-02-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2022"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            complement(join(6387..7135,1..751))
                     /gene="csa"
                     /locus_tag="V6S26_RS01560"
                     /old_locus_tag="V6S26_01560"
     CDS             complement(join(6387..7135,1..751))
                     /gene="csa"
                     /locus_tag="V6S26_RS01560"
                     /old_locus_tag="V6S26_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="WP_251295019.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFLATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            990..1280
                     /locus_tag="V6S26_RS01565"
                     /old_locus_tag="V6S26_01565"
     CDS             990..1280
                     /locus_tag="V6S26_RS01565"
                     /old_locus_tag="V6S26_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024216191.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(1272..1550)
                     /locus_tag="V6S26_RS01570"
                     /old_locus_tag="V6S26_01570"
     CDS             complement(1272..1550)
                     /locus_tag="V6S26_RS01570"
                     /old_locus_tag="V6S26_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1672..2094
                     /locus_tag="V6S26_RS01575"
                     /old_locus_tag="V6S26_01575"
     CDS             1672..2094
                     /locus_tag="V6S26_RS01575"
                     /old_locus_tag="V6S26_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_024216190.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2577..2810)
                     /locus_tag="V6S26_RS01580"
                     /old_locus_tag="V6S26_01580"
     CDS             complement(2577..2810)
                     /locus_tag="V6S26_RS01580"
                     /old_locus_tag="V6S26_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2817..3377)
                     /locus_tag="V6S26_RS01585"
                     /old_locus_tag="V6S26_01585"
     CDS             complement(2817..3377)
                     /locus_tag="V6S26_RS01585"
                     /old_locus_tag="V6S26_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3412..4092)
                     /locus_tag="V6S26_RS01590"
                     /old_locus_tag="V6S26_01590"
                     /pseudo=""
     CDS             complement(<3412..4092)
                     /locus_tag="V6S26_RS01590"
                     /old_locus_tag="V6S26_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4055..4378)
                     /locus_tag="V6S26_RS01595"
                     /old_locus_tag="V6S26_01595"
     CDS             complement(4055..4378)
                     /locus_tag="V6S26_RS01595"
                     /old_locus_tag="V6S26_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4446..4637
                     /locus_tag="V6S26_RS01600"
                     /old_locus_tag="V6S26_01600"
     CDS             4446..4637
                     /locus_tag="V6S26_RS01600"
                     /old_locus_tag="V6S26_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024192883.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(<4792..4977)
                     /locus_tag="V6S26_RS01605"
                     /old_locus_tag="V6S26_01605"
                     /pseudo=""
     CDS             complement(<4792..4977)
                     /locus_tag="V6S26_RS01605"
                     /old_locus_tag="V6S26_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5306..5419
                     /locus_tag="V6S26_RS01610"
                     /old_locus_tag="V6S26_01610"
     CDS             5306..5419
                     /locus_tag="V6S26_RS01610"
                     /old_locus_tag="V6S26_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_213849828.1"
                     /translation="MHEPPVQPDRCALSGNYRLESTPVRHDKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            complement(5635..5790)
                     /gene="csl"
                     /locus_tag="V6S26_RS01615"
                     /old_locus_tag="V6S26_01615"
     CDS             complement(5635..5790)
                     /gene="csl"
                     /locus_tag="V6S26_RS01615"
                     /old_locus_tag="V6S26_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="WP_071703583.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            5820..6359
                     /gene="csi"
                     /locus_tag="V6S26_RS01620"
                     /old_locus_tag="V6S26_01620"
     CDS             5820..6359
                     /gene="csi"
                     /locus_tag="V6S26_RS01620"
                     /old_locus_tag="V6S26_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="WP_104807860.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(4723..4810)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4834..5378
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.343"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2471..4065)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4438..4640)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.39e-10"
ORIGIN
        1 ctttttcact ccctacataa ggacgaacag ctgttctagt tacagataaa acattaccat
       61 aatcatcaag caccactaaa tagtggaact gttcaacatc aacatcaaat tttctcacat
      121 taggaggcaa acgatcccac gcatcttcag cctgttgttt tgctttttcc tgccgctgag
      181 attcatcttt attaaaacct aacgcctcat atatctcctc aggagtttta tgattgattt
      241 tttccataat acccgcctta gctttttcac tccctacgta aggacggaca cctgttcgaa
      301 gaataccgat agctctacca ttatcattaa gcgttacacg atatttaaaa ccatcgatac
      361 tcacatcaat ctctcgcggt aagttgtttt tatcccactt acgagcaact tcatcattag
      421 gattttcagg gatatagccc ccctgaacat cactatttcc gccagcccca ggaactccat
      481 atacaggcgt ttttaccaga ccatttgtga tggtaatgcg cacctgccca ttatcatcaa
      541 cataaggcgg cacatccgga ttattggttt tactccatcc ccaccccgtc tgacttccat
      601 gttcacgacc tccattacca gagcctgaac caccacccca gtgaacccca ctattactat
      661 tattatttcc accttgctga ttcagattcg cccctgttcc ccccatagac tcaccagcag
      721 ttgggccata tccacttagt tctttagcca taaattcctc tttgataatt aaaacaataa
      781 attaaaaaca gaacactgta catataacca ctggttttat gtacagcaaa acctactact
      841 cactattgtc catgtcaaga gcatggattt tcatttttgc tataaggatc acgctatggg
      901 gaggcaggca ttgagaacgt cgaaacagaa caccggagca aatcaggatg agatataaaa
      961 ctgttggatc atgaaaaacg gagaacgatg tgagcaaatc accccgccat aaactgaaca
     1021 aaacagacaa acgacttctc gacacccttg ttgctgccgg atatgagcat gacaaagccc
     1081 gtgacctcat ccagaaacag gtttacacgc tgacactggc tgatcagcgt catgtggtca
     1141 gtgaaatcag taatggtgtg aatcccaccc aggcttactc ggcggtatac caggcaagac
     1201 gcattcgcct cgcccgtaaa tatctgaacg gtaaaaaggt tatggaagaa accggggaaa
     1261 atacgccccc atcaacgtaa ggatttcttt tgccgctcca gagactccag ttttttacgc
     1321 aaatcctctc ttttttgggc atctctggtg ccagccagct ctgctctcaa ctcatcgatc
     1381 tgaagttgta tcttcagtct gttactgaac attttctgtc tggcattaac atccgcaaca
     1441 atgccgtttt ttgtcttctc ggccttttgt tgaaaaacat tgctgtccgc atgactggca
     1501 accgaagcag aaagaacact aaaaagcagg actggcacac attttttcac gggattattc
     1561 ctgactcatt gaccatcaaa tcacattggg agtaaaccga cgtatgataa gagatactct
     1621 tcggagatat aactccctga gtatcaagat taaaaacgca aggagatgtt tatgagatct
     1681 gccgctacca ggctgcttct gatacctctg ataacagcaa caatagctct tacaggatgc
     1741 accccaaaga ccagcctgga acgacatacc cgacattatg tttatgcttc agatgatgga
     1801 tttgatccta acttctatac ccagaaagca gacaccatac gtatgatgct cccgttcttt
     1861 cagcagttcc gggatatggg gatgaaagac aaagcagccg gagtatcagc agaaacggca
     1921 cagcaacgtg caaaagaatt ccactcagaa aaattttttc actcactcca gagcacaaca
     1981 acctttgctg gcagaaaata cacaaacagc gatatgcctt cgctgaaaaa aatgaaacta
     2041 atggcagaca ccatttctgc ggtttatctc gatggatacg agggcagaca gtaagggatt
     2101 taccataatc ccttaattgt acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg
     2161 acaggtaaaa atggcaacaa accacccgaa aagctgccgc gatcgcacct gataaatttt
     2221 aaccgtatgc atagctattc agccatgtga ataacgctgg ttttgcctgc gtaaacctca
     2281 tgacactgtt tttttccatc ttttcagttg atgacatacg cagacatcgc gggatgaggc
     2341 tgaggaatga gcgcgatctg gcaaagaggc aaaacacagc aacaaaaacg acacgccaga
     2401 atcgcgcccg gatgcgtttt taacgcgttc cggtaccatc tggcaacctc ccggaacaac
     2461 tcaccgtcac atccccattg acgggccacg ccatacctgt gcttcccgtt cctgctcttc
     2521 atgccaggac cgcgcacgct cccgttccag gcgtgcctgc ctttcctgtt catcccttat
     2581 ctgctgttcg tgataaataa ccgactcaag cggtccacct gcccggctaa tctctgcacc
     2641 tgctgactca agtcgtcgca ctgttccctc agttgcccgt tctcctgtcg tgtcagctcg
     2701 aacatatgct gcaaatccgt gaaggcgctc tcccagtctt tcagccgctg cgtatagtcc
     2761 tgctgcaatt gctctaaggc gttcagtaag tgcatttcca gctctgtcat actcacttac
     2821 tccctgacca gtcttactgc gttcttcttc tccaccgtcc agttgttttc ccccttcacc
     2881 ccggacggca gcaccagaaa tttcccgttc ctgccctcgt gatacgtcac accccatgtt
     2941 ttttcccgga gtttcgccag cgtctcttcc tggtccctga tagccaggat gttcgccgca
     3001 atccggcttt cctgccactg aatcagcccc catgacgcca gaaaaatccc gcccgtgacg
     3061 caaagcgccg tcagcgacgg gtacagtatc cgccctttga tcagcttcca gagcagctct
     3121 tcctgccgcc gggccagttc gttctctgtg gcgctgaact gcgcgttcac ggcactgttc
     3181 agcgtctcca gttgttcttt caccgctgct gtgtgtgcgc tgatagcgtc tctgattttc
     3241 tgcccgttta agttcagttc cctgtctaca gacgcttcga gcttcctgaa ctcgctgttc
     3301 agcatgttct ctgtagagac ggcacgctct ttcagtttct tctcgaagtc tgtccccatt
     3361 tgtaaaagat tgctcataca gcgccccttt cagcctgaga ttacgcccgc cctccgggtc
     3421 ggcgatactg atactgctcc tggttgtcct cacaacctca aaacctgccg ctgtaagcgc
     3481 ctcagtgaca tcctgacgcg tttttagcgc tccggcatgg taaagagcct ccagtcccct
     3541 cgtaatcgct tctgcggcct cctgtttcgc tttcggcaga ttattcgggg tgacaagtgt
     3601 ccgcctgttc tccggtgcgt tcgggtcgtg cagcccgtaa tggtgattca ccagtgtctg
     3661 ccaggcattg attcgcggac ggtccgcccg gtcgtaatag ggctggagcc gttttccgct
     3721 cgccagctcc atattcggga tgacaaaatt cagctcaaga cgccccttgt cctggtgctc
     3781 cacccacagg atgctgtact gatttttttc aagaccgggc atcagtaccc gctcaaagct
     3841 ctccatcacc ctttcacgct ctcccggtgg cagggtctgc tctgcaaaag acagaacccc
     3901 cgaggtgtat tttttcgcaa acggcgtggc atcgatgagt tcccgtacct cttcgggagc
     3961 accccgcaga actctcgccc cttcccggtt acgctcccgg cccagcaggt aatcaaccgg
     4021 accactgcca ccgccttttc ccctggcatg aaacttaact atcatcccgt tctccctgtt
     4081 tacggacctc atccctaagc tcactcagtt cacgtccgat ggccatcagt gcagccacca
     4141 catgaacccg gtcatgcccc gaccactgtc cgctgtttat cttcctggct atctgattca
     4201 ggttattgcc gaccgaagcg aactggcgca acagcggcgg tgccagtgtc ggaagacctg
     4261 acgttttcga tggcggtgcc cccaggcaga ccttacgcat ccatgacgca agttgttttc
     4321 cctcacaacg tgccagcagc cgcgcatgtt cctcatccgt gacccgtatc gtgagcatcc
     4381 tttcgcgttt caccggtatc attaaaaacc tccgacagac tccccacaca tggagaaaca
     4441 gaactgtgac taaacaggaa aaaaccgccc ttaacatggc ccgcttcatc agaagccaga
     4501 cgctgaccct gctggaaaaa ctgaatgaac tggacgtcga cgaccaggct gacatctgcg
     4561 aagcgcttca cgaccacgct gacgagcttt accgcagctg cctcgcacac ttcggggatg
     4621 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagctggc ctgtgagcgg
     4681 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg ttttagcggg tgtcggggcg
     4741 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaatc gtgtagcatc
     4801 ggtgaggatt gtatgaaaag tgcaccatgc cgggtgtgaa atgccgcaca gatgcgtaag
     4861 gagaaaatgc tcgtccaggc gcttttccgc ttcctcgctc actgactcgc tgcgctcggt
     4921 cgttcgactg cggcgagcgg agtctgctgg ctcaaaaacg gcgatacggt tatccacaga
     4981 atcaggggat aaggcagaaa agaacatgtg agcaaaaagg caaagggcaa aaaaagccgc
     5041 gtagctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     5281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5341 cttatccggt aactatcgtc tcgagtccac cccggtaaga cacgacaaag cgccactggc
     5401 agcagccatt ggtaactgaa aagtggattt agatacgcag aactcttgaa gttgaagcct
     5461 tatagcggct acactgaaag gacagcattt ggtatctgtg ctccacttaa gccagctacc
     5521 cggttaagca gtccccaact gacttaacct tcgactaaac cgcctcccca ggcggttttt
     5581 tcgtttacag ggcaagagac tacgacgatc tgtaaaggat ctcaagaaga tcctttacag
     5641 gctaccacta ccaccacttc ccatactcac tccggtaaca gtggaagatg gtgaaacagc
     5701 cccacctcca gtatctctga cattgttgac ttggcatgct gcaagcagca ttattcctag
     5761 tgaaatggca caaataataa tttttttcat atataccttt attataatcc tataggtcaa
     5821 tgatgattga tgaacattca atagatatag acaatagaaa agccaataac ttattgtatt
     5881 tatttatggc aatcggagtt atacctttac tctgtatact agccgtttac tatacaaatc
     5941 cagacaatct gttcttgcac acaatagcta ccagtactga aaacatccca tcaataacct
     6001 ccgcctacaa cccattaatg acaaaggtta tggatattta ttgtaaaaca gcgccttccc
     6061 ttgctttgat attatttatc ctgacattta aaacgagaaa gctaataaaa aaaacaaacc
     6121 gaaatgctgt acttagaagt tgcttattaa gtccatttgt ctgtgctgtt tttttgtatt
     6181 tactctgttt ccgaaacctt gagctaacga cagcaggaag gccagctagg ctaatgacca
     6241 acaatgatgc aacactattg ctattttata ttggcctgta cttaatcatt tttttcataa
     6301 gctatttcac gctattcaca ccagttacca cgtttaaatt attaaaagaa aggcagtaac
     6361 cctgccttcc ttttacccgg tatttattaa tgagcgggtt taatgatttc tttatttagt
     6421 gcatctgcaa acttgtcatc aattaatgca cctacaactg ctgcgagtag aatacctacg
     6481 ataccgacag caatagcagg agccccaaga gataaggcat atgcccctaa tgtggctaaa
     6541 aaaacaccca gggccacagc agaagcgatg ccactgagca cccaggactc cacctccagc
     6601 atcagaggtc cccagttccc ggtttcatag ccctcgatgc ttttctcccg gaccttctca
     6661 accttcatca ccacatcggc aaatttgaaa gccttgctga tattacccag tttatttgcc
     6721 atatcctgag catcaatact tcgccagacg ttcgccagtg catcactgtc ggccctgtta
     6781 aatttaaacc ccggattaga caaaacttta tttaatgacg ccattgcatc atcgtagcta
     6841 cggatagttt tcccctggaa atttttgaca tcatcagcaa tttctcttgc taacgactta
     6901 taagcatctc caagataatc accgacctta ttacctatat cagagataac ttcagcggct
     6961 ttattaagta catcactctg aatccggtca cggttcatac tgaaagaata aaacacatca
     7021 taagcagctt ttttagcttg atcctgtcgt tgaggctctt cattattaaa tcctaatgcc
     7081 tcatatatct cttccggggt cttatggtcg actttatcca taataccagc cttag
//
