LOCUS       NZ_CP145702             4074 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-CAM-04-C plasmid p2-S1-CAM-04-C, complete
            sequence.
ACCESSION   NZ_CP145702
VERSION     NZ_CP145702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052294
            Assembly: GCF_036884775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 251.1075X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884775.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:21:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,257
            Genes (coding)                    :: 5,064
            CDSs (with protein)               :: 5,064
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 60 of 193
            Pseudo Genes (incomplete)         :: 140 of 193
            Pseudo Genes (internal stop)      :: 30 of 193
            Pseudo Genes (multiple problems)  :: 38 of 193
            Pseudo Genes (short protein)      :: 4 of 193
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-CAM-04-C"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2-S1-CAM-04-C"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2022"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            join(3156..4074,1..1247)
                     /gene="mobQ"
                     /locus_tag="V6S27_RS00900"
                     /old_locus_tag="V6S27_00900"
     CDS             join(3156..4074,1..1247)
                     /gene="mobQ"
                     /locus_tag="V6S27_RS00900"
                     /old_locus_tag="V6S27_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029349.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRAQL
                     LKREDELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFESVDLIRDRSGYICDRSEIGQHPPLQRIYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAVVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRPDRGWSQGM"
                     /name="mobQ"
     gene            complement(1489..2190)
                     /locus_tag="V6S27_RS00905"
                     /old_locus_tag="V6S27_00905"
     CDS             complement(1489..2190)
                     /locus_tag="V6S27_RS00905"
                     /old_locus_tag="V6S27_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2723..2977)
                     /gene="mobC"
                     /locus_tag="V6S27_RS00910"
                     /old_locus_tag="V6S27_00910"
     CDS             complement(2723..2977)
                     /gene="mobC"
                     /locus_tag="V6S27_RS00910"
                     /old_locus_tag="V6S27_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2979..3155)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3156..3514
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="100.0"
                     /evalue="8.52e-174"
     Typing          complement(1491..2190)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.287"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..1238
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.087"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3070..4073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.91"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgaacaccga acgaaaggaa gcgatgagcc atgctgccag cctgaaaaaa tcatcgccgt
       61 tttcgtccgg tttttcccgt attcccctga tgcaatgggc tgcaccggaa taccgtagtg
      121 agcagggaaa aatccagtct ctgagccagc aaatggaaaa attgcgacag cattaccgga
      181 aggtggagaa acaggacatt cccgcgagtc agacggcatt tgaaaaacag tggcagcagt
      241 ggggggaatc cggccttgca gctctcagag cccagttgct taaacgtgaa gatgaattac
      301 gccggaagga gctggaggat acagaacgta gtcgcgcgga gcgacgagcg gaacagctca
      361 ggaaaaatga caatgccgtt atggattcag gggtgctgag agcagtcgtt acggggtatg
      421 gcgaggctcc catgcctggt aacggggaaa tgacttgtta ccttctgctt cacaaccgga
      481 acggagaata cacgctctgg gggaacgagc tggagaaata ccgcaccagg atctttgaga
      541 gtgttgattt aatacgtgac aggagtggtt atatctgtga ccgaagcgaa atcggccaac
      601 atccaccact gcaacgtatt tattcctctg cgacctttga gcaacttctg acgcaggttt
      661 gccagacatg gccgcaacat acccgcaatc tccgccagcc gaagacatgg ccggagtctt
      721 tctgtcttgg agaagaccgc cagccagcca tgccatcact ggcagcaagg aaggtggatt
      781 ttacgcaggg acgtctgcca ccgacgctga tgccggtcat gagttctgtc gaccgggaaa
      841 cccgccagct tcagcttttg ctggtcatgg gcgtggatga ctctctgggc ggtgttgtcc
      901 ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc agccgataac agccagctgg
      961 ttatcagtgc actgaccgat aaggggttac ggtatgcagg gtatggtgtt gccgtaaatc
     1021 atgatgcaga cagccacata tcacctgcac cggaacttat ggagtttcat ctgaaaaccc
     1081 gtgaggcacc gctttttgct gtcgtcaaca caccagaaaa acagccggat catctgttcc
     1141 gctcgctggg tttcaatcgt acctgggacg aatggcggcg cgaggaagat gcacgaacac
     1201 acgccaccga gaggagacct gatcgcggtt ggtcacaggg aatgtgaaca atattttgtt
     1261 ttgtttaaca tcgtgttatg ttcgtcgatg ttcatcgaca gggatgaatg caagaagcac
     1321 actcacatag cgcaaacata gttttgtttt tccgccccca aaaattgggg gttctttttt
     1381 tgctgcatca gagcgttttt gacgcaactt ttcacttcaa tagtaccttc gcattcctga
     1441 cacaagaaaa acgcgacagt gagcgtttta ggtgccttaa aatcgattct agatcgtcat
     1501 cttgatctgt ttgtcttcct tgaagcgaaa gtgcagggct acaaccgtgc gccccttttt
     1561 gatcgtctca accgttacaa ccagatcgct tttttcgttc agttcggcga tggctggctt
     1621 gattatcatc ttattcaagt ctttgaattg ttgatattta tcttcaacgg caagcatggt
     1681 tcggaaatca tcgatagcta tcgttctgtc accggtgctg cggaactgct ggagcagctc
     1741 gtaaatgcgg atgctgtagg tgctggagag gctggagacg ttcttcacga ctacccgcgt
     1801 aaactgcccc ttgagctgtg tcagatacgg catgatggca tcggagaagg tgatctctac
     1861 tttcgcctca cctttgaaat actgcgcccg gtactgcacc cagcggaact cttctcgttt
     1921 ttcctcgttc ttgatgatta ctgacctgtc ccagaggcga tctatcgctt ccttgagcct
     1981 agcttcagcg tgctgccgtc ccatgtcagg aaaggcatca tgaaactctg cagccgtgat
     2041 ggtcatggtt ttttgttcat ccggcgaagg ggaatcttct ccggatttca ggcggctgat
     2101 acagaccagc aggaagcgct gttcctgcaa agtgagcttg tagcttgcct cgataagggc
     2161 gtttgatttg taagctatca gatctgacat ttcgcttgct cgttgctatt ggtggcgttt
     2221 ttttactgta cgccacctat catattggtg gaagtttttt ttttgcaacc ccttttttct
     2281 gtggattaca cccccaaaag ctccacttat cacccccaaa agctccacct atcaccccca
     2341 aaagctccac ctatcacccc caaaagctcc acctatctcg ccagaacacc tttaaaatca
     2401 tgtggttaca agacctaaaa aggttttaaa aaaaaagatc taaaaataag atcttaaaaa
     2461 cttaacggcg agcctgtgga taacttgggg tgctgaaaat aaaaatccaa taaaaacaat
     2521 gtgttgcaaa aaaccaggat tgaagattcc gaatacaccc cctccaaccc tgtggaaatc
     2581 cagtattcat gcgggttcag cgcatatcac ctgtcgaaaa accgtgcgta tgtgctcaac
     2641 atggcgactg attcacttcc gcttcctcgc ccactcgctc gctaacgctc ggtcgctggg
     2701 ctgcggcgag cggacgatac aatcactgct ctggcagcgc ctccagctcg ttaatcagcg
     2761 gcagtattcg cgtcacatca gcctgccgtg tcacctctgc cttcaggaac tggagtaacc
     2821 aggctcgcaa ctgcacttcc tccggctttc tgacctttgc cagcaatgct gcacccacaa
     2881 tgaccttctg cgctgtgtcc aggctccggc tcttcgcctt caggcgctgt aatctcgcct
     2941 cagcatcagc aatcttctgt tcgagcgttc tgctcatgat gtaactccat gcgcgatgaa
     3001 aaatcgcatt ttagcgcgtc actggtagtt taaaaactga gctggcataa tgcacggcaa
     3061 atcacgaagt gcgcacttat acaatctcca ctccgtttcg attgtgtgcg ccctgcgggg
     3121 ctgaaagaaa actgcaaaaa ggcattacgg cagaaatggc gatttatcat ctcagtatga
     3181 aaatcatctc cagaagcaac ggctacagcg ccgttgcttc tgctgcctat cgttctggtt
     3241 cactaatgct tgatgagcgt accggactga ctcacgacta cacccgcaaa agcggtgtgg
     3301 ctgaggctgt gattctgacc cccgcgacag cccctgcctg gtgtacgaac cgtgccgaac
     3361 tctggaatgc cgttgagaag gccgaacgcc ggaagaactc acaacttgcg cgggaaattg
     3421 aactcgccat cccccacgaa ctgccgcagg atgccgcacg ggaaaccgtt ctcgcctttg
     3481 tcagagaaaa ctttgtcagt cagggcatga ttgctgatgt ggcgttccat cacatggata
     3541 aaacaaatcc ccatgcgcac attatgctga ccacgagggc tgtcggacct gcggggttcg
     3601 gcggaaaggt cagggactgg aacgaccgga cacatgccga gacgtggcgt gcatcatggg
     3661 ctgaccatgc aaacagggca ctggcgaacg ccggttatca gaaagagata gaccatcgtt
     3721 catacgagcg acaggggctt gagaaaacgc cgggtattca cctcggaaag tctgcctgtg
     3781 cgatggaaac acgcggtatc gaaacagaac gcggcgaaca gaatcgtctg attaacaggc
     3841 ttaacctgga aatacagatt tcccgcaccg aactcaggaa cagcgggctg acagagcctg
     3901 cccgccgtgt ggctgacctt ctgaatatcg ggcttcctga caacgcaact tcgtacacac
     3961 tacgtgacat catcgaagcg ttgccgaagg acagtaacgc ggcatggaaa ctcaccagta
     4021 acgccatgtc gatgatggca gatatgcagg cgacgcgcag acgctgggaa gagc
//
