LOCUS       NZ_CP145696             6995 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-KEN-03-A plasmid p2-S1-KEN-03-A, complete
            sequence.
ACCESSION   NZ_CP145696
VERSION     NZ_CP145696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052296
            Assembly: GCF_036884755.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 171.4162X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884755.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:15:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,154
            CDSs (total)                      :: 5,034
            Genes (coding)                    :: 4,844
            CDSs (with protein)               :: 4,844
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 66 of 190
            Pseudo Genes (incomplete)         :: 124 of 190
            Pseudo Genes (internal stop)      :: 40 of 190
            Pseudo Genes (multiple problems)  :: 40 of 190
            Pseudo Genes (short protein)      :: 4 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-KEN-03-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2-S1-KEN-03-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            complement(join(6370..6995,1..1027))
                     /gene="cka"
                     /locus_tag="V6S29_RS00735"
                     /old_locus_tag="V6S29_00735"
     CDS             complement(join(6370..6995,1..1027))
                     /gene="cka"
                     /locus_tag="V6S29_RS00735"
                     /old_locus_tag="V6S29_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_001554611.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSNSGVHWGGGSG
                     HGNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVSNVINEK
                     QKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNS
                     YKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAE
                     SDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENEL
                     KNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIA
                     QDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTK
                     ISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVG
                     VPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            1268..1558
                     /locus_tag="V6S29_RS00740"
                     /old_locus_tag="V6S29_00740"
     CDS             1268..1558
                     /locus_tag="V6S29_RS00740"
                     /old_locus_tag="V6S29_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(1550..1828)
                     /locus_tag="V6S29_RS00745"
                     /old_locus_tag="V6S29_00745"
     CDS             complement(1550..1828)
                     /locus_tag="V6S29_RS00745"
                     /old_locus_tag="V6S29_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1950..2372
                     /locus_tag="V6S29_RS00750"
                     /old_locus_tag="V6S29_00750"
     CDS             1950..2372
                     /locus_tag="V6S29_RS00750"
                     /old_locus_tag="V6S29_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2856..3089)
                     /locus_tag="V6S29_RS00755"
                     /old_locus_tag="V6S29_00755"
     CDS             complement(2856..3089)
                     /locus_tag="V6S29_RS00755"
                     /old_locus_tag="V6S29_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001554706.1"
                     /translation="MTELEMHLLNALEQLQQDYMQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3096..3656)
                     /locus_tag="V6S29_RS00760"
                     /old_locus_tag="V6S29_00760"
     CDS             complement(3096..3656)
                     /locus_tag="V6S29_RS00760"
                     /old_locus_tag="V6S29_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3691..4371)
                     /locus_tag="V6S29_RS00765"
                     /old_locus_tag="V6S29_00765"
                     /pseudo=""
     CDS             complement(<3691..4371)
                     /locus_tag="V6S29_RS00765"
                     /old_locus_tag="V6S29_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4334..4657)
                     /locus_tag="V6S29_RS00770"
                     /old_locus_tag="V6S29_00770"
     CDS             complement(4334..4657)
                     /locus_tag="V6S29_RS00770"
                     /old_locus_tag="V6S29_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4725..4916
                     /locus_tag="V6S29_RS00775"
                     /old_locus_tag="V6S29_00775"
     CDS             4725..4916
                     /locus_tag="V6S29_RS00775"
                     /old_locus_tag="V6S29_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001554612.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5584..5727
                     /locus_tag="V6S29_RS00780"
                     /old_locus_tag="V6S29_00780"
     CDS             5584..5727
                     /locus_tag="V6S29_RS00780"
                     /old_locus_tag="V6S29_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(5922..6053)
                     /locus_tag="V6S29_RS00785"
                     /old_locus_tag="V6S29_00785"
     CDS             complement(5922..6053)
                     /locus_tag="V6S29_RS00785"
                     /old_locus_tag="V6S29_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            6083..6373
                     /gene="cki"
                     /locus_tag="V6S29_RS00790"
                     /old_locus_tag="V6S29_00790"
     CDS             6083..6373
                     /gene="cki"
                     /locus_tag="V6S29_RS00790"
                     /old_locus_tag="V6S29_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     Typing          complement(5002..5089)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5100..5656
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2750..4344)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4717..4919)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="3.93e-12"
ORIGIN
        1 cattcttaag ttcattttca actgcggagt actcagcctg cttagcttca agctgacgct
       61 gtagtttttt ctgaacgtca cgccatcccg gaaatccggt gaccttcacg tcaacactct
      121 tctgagcgtt ttcaagctct cctgcaactc tggaaacagt agcttgctta ctctgtacat
      181 cactttcagc cttcgccagt tcagctttcg cctcagctaa acgcttctct gcttccgcca
      241 ccgcctgttt ctcttcatta agagtttcat gttcagcgac ctctgcattt tcacgagcct
      301 tttcttcagc ttccttctgt ttattcacat caccaatttt actttgcaat gtatttttat
      361 aagaattaag tttgctaata tctgcatcaa gctgatttga ttttttttgc aactcatcaa
      421 catccctctc aagatcagtg ataccatgat aagaatgatg cttgaatact tttttcatct
      481 cctcgatttt cttctgtttt tcactaatct gtgtagcaat tttattcttc tgcttttgtt
      541 tttcattaat tacattactc accaccttcg aactcttatc catatcactg acctgagcat
      601 tcgttggtgc agtattaact gaagcagtgt tattgttgtt atttcctgag ctttctgcaa
      661 aaagtgatgg catatcacta attaaagaat taagaactct ggagacccct ccaaatggat
      721 tatcaaccag agttgaattc tcttctgtca taacaatacc attcatcacc ggaaggccat
      781 cattattaat gacaacatca ccccacggag tcagatatga ctcaccggtt ttcatcacag
      841 ttgatgtaga accagatgaa tttgaatttc cctgaccacc attattacca tgcccagagc
      901 caccacccca gtgaacccca ctattactat tactattatt atttccacct tgctgattca
      961 gattcgcccc tgttcccccc atagactcac cagcagttgg tccatatcca cttagttctt
     1021 tagccataaa ttcctctttg ataattaaaa caataaatta aaaacaatat actgtacata
     1081 taaccactgg ttttatgtac agtaaaaacc tactactcag cattgtccat gtcaagagca
     1141 tggattttca tttttgcaat aaggatcaca ctatggggag gcaggcattg agaacgtcga
     1201 aacagaacac cggagcaaat caggatgaga tataaaactg ttggatcatg aaaaaacgga
     1261 gaacgatgtg agcaaatcac cccgccataa actgaacaaa acagacaaac gacttctcga
     1321 cacccttgtt gctgccggat atgagcatga caaagcccgt gacctcatcc agaaacaggt
     1381 ttacacgctg acactggctg atcagcgtca tgtggtcagt gaaatcagta atggtgtgaa
     1441 tcccacccag gcttactcgg cggtatacca ggcaagacgc attcgcctcg cccgtaaata
     1501 tctgaacggt aaaaaggtta tggaagaaac cggggaaaat acgcccccat cagcgtaagg
     1561 atttcttttg ccgctccaga gactccagtt ttttacgcaa atcctctctt ttttgggcat
     1621 ctctggtgcc agccagctct gctctcaact catcgatctg aagttgtatc ttcagtctat
     1681 tactgaacat tttctgtctg gcattaacat ccgcaacaat gccgtttttt gtcttctcgg
     1741 ccttttgttg aaaaacattg ctgtccgcat gactggcaac cgaagcagaa agaacactaa
     1801 aaagcaggac tggcacacat tttttcacgg gattattcct gactcattga ccatcaaatc
     1861 acattgggag taaaccgacg tatgataaga gatactcttc ggagatataa ctccctgagt
     1921 atcaagatta aaaacgcaag gagatgttta tgagatctgc cgctgccagg ctgcttctga
     1981 tacctctgat aacagcaaca atagctctta caggatgcac accaaagacc agcctggaac
     2041 gacatacccg gcattatgtt tatgcttcag atgatggatt tgatcctaac ttctacaccc
     2101 agaaagcaga caccatacgt atgatgctcc cgttctttca gcagttccgg gatatgggga
     2161 tgaaagacaa agcagccgga gtatcagcag aaacggcaca gcaacgtgta aaagaattcc
     2221 actcagaaaa attttttcac tcactccgga gcacaacagc ctttgctggc agaaaataca
     2281 caaacagcga tatgccttcg ccgaaaaaaa tgaaactaat ggcagacacc atttctgcgg
     2341 tttatctcga tggatacgag ggcagacagt aagggattta ccataatccc ttaattgtac
     2401 gcaccgctga aatgcgttca gcgcgatcac ggctgctgac aggtaaaaat ggcaacaaac
     2461 cacccgaaaa gctgccgcga tcgcacctga taaattttaa ccgtatgcat agctattcag
     2521 ccatgtgaat aacgctggtt ttgcctgcgt aaacctcatg acactgtttt ttttccatct
     2581 tttcagttga tgacatacgc agacatcgcg ggatgaggct gaggaatgag cgcgatctgg
     2641 caaagaggca aaacacagca acaaaaacga cacgccagaa tcgcgcccgg atgcgttttt
     2701 aacgcgttcc ggtaccatct ggcaacctcc cggaacaact caccgtcaca tacctattga
     2761 cgggccacgc catacccgtg cttcccgttc ctgctcttca tgccaggacc gcgcacgctc
     2821 ccgttccagg cgtgcctgcc tttcctgttc atcccttatc tgctgttcgt gataaataac
     2881 cgactcaagt ggtccacctg cccggctaat ctctgcacct gctgactcaa gtcgtcgcac
     2941 tgttccctca gttgcccgtt ctcctgtcgt gtcagctcga acatatgctg caaatccgtg
     3001 aaggcgctct cccagtcttt cagccgctgc atatagtcct gctgcaattg ctctaaggcg
     3061 ttcagtaagt gcatttccag ctctgtcata ctcacttact ccctgaccag tcttactgcg
     3121 ttcttcttct ccaccgtcca gttgttttcc cccttcaccc cggacggcaa cactagaaat
     3181 ttcccgttcc tgccctcgtg atacgtcaca ccccatgttt tttcccggag tttcgccagc
     3241 gtctcttcct ggtccctgat agccaggatg ttcgccgcaa tccggctttc ctgccactga
     3301 atcagccccc atgacgccag aaaaatcccg cccgtgacgc aaagcgccgt cagcgacggg
     3361 tacagtatcc gccctttgac cagcttccag agcagctctt cctgccgccg ggccagttcg
     3421 ttctctgtgg cgctgaactg cgcgttcacg gcactgttca gcgtctccag ttgttctttc
     3481 accgctgctg tgtgtgcgct gatagcgtct ctgattttct gcccgtttaa gttcagttcc
     3541 ctgtctacag acgcttcgag cttcctgaac tcgctgttca gcatgttctc tgtagagacg
     3601 gcacgctctt tcagtttctt ctcgaagtct gtccccattt gtaaaagatt gctcatacag
     3661 cgcccctttc agcctgagat tacgcccacc ctccgggtcg gcgatactga tactgctcct
     3721 ggttgtcctc acaacctcaa aacctgccgc tgtaagcgcc tcagtgacat cctgacgcgt
     3781 ttttagcgct ccggcatggt aaagagcctc cagtcccctc gtaatcgctt ctgcggcctc
     3841 ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct ccggtgcgtt
     3901 cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga ttcgcggacg
     3961 gtccgctcgg tcgtaatagg gctggagccg ttttccgctc gccagctcca tattcgggat
     4021 gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga tgctgtactg
     4081 atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc tttcacgctc
     4141 tcccggtggc agggtctgct ctgcaaaaga cagaaccccc gaggtgtatt ttttcgcaaa
     4201 cggcgtggca tcgatgagtt cccgcacctc ttcgggagca ccccgcagaa ctctcgcccc
     4261 ttcccggtta cgctcccggc ccagcaggta atcaaccgga ccactgccac cgccttttcc
     4321 cctggcatga aacttaacta tcatcccgtt ctccctgttt acggacctca tccctcagct
     4381 cactcagttc acgtccgatg gccatcagtg cagccaccac atgaacccgg tcatgccccg
     4441 accactgtcc gctgtttatc ttccgggcta tctgattcag gttattgccg accgaagcga
     4501 actggcgcaa cagcggcggt gccagtgtcg gaagacctga cgttttcgat ggcggtgccc
     4561 ccaggcagac cttacgcatc catgacgcaa gttgttttcc ctcacaacgt gccagcagcc
     4621 gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct ttcgcgtttc accggtatca
     4681 ttaaaaacct ccgacagact ccccacacat ggagaaacag aactgtgact aaacaggaaa
     4741 aaaccgccct taacatggcc cgcttcatca gaagccagac gctgaccctg ctggaaaaac
     4801 tgaatgaact ggacgccgac gaccaggctg acatctgcga agcgcttcac gatcacgctg
     4861 acgagcttta ccgcagctgc ctcgcacgct tcggggataa cggtgaaaac ctctgacaca
     4921 tgcagctccc ggagacggtc acagctggcc tgtgagcgga tgccgggagc agacaagccc
     4981 gtcagggcgc gtcagcgggt tttagcgggt gtcggggcgc agccatgacc cagtcacgta
     5041 gcgatagcgg agtgtatact ggcttaatca tttggcatca gtgaggattg tatgaaaagt
     5101 gcaccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgct cgtccaggcg
     5161 cttttccgct tcctcgctca ctgactcgct ccgctcggtc gttcggctgc ggcgagcggt
     5221 atcagctcac tcaaagacgg taatgcggtt atccacagaa tcaggggata acaccaaaag
     5281 aaacatgtga gcaaaaaaca agaacccgga aaaggccacg cagctggcgt ttttccatag
     5341 gctccgcccc cctgacgagc atcacaaaaa ccgacgctca agtcagaggt ggcgaaaccc
     5401 gacaggactt aaagatacca ggcgtttccc cctggtggct ccctcgtgcg ctctcctgtt
     5461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc ctttgggaag cgtggcgctt
     5521 tctcatagct cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5641 gagtccaacc cggtaagaca cgacttaacg ccactggcag cagccattgg taactggata
     5701 gtggatttag atacgcagaa ctcttgaagt tgaagcctta tagcggctac actggaagga
     5761 cagcatttgg tatctgtgct ccactaaagc cagttacccg gttaagcagt ccccaactga
     5821 cttaaccttc gactaaaccg cctccccagg cggttttttc gtttacaggc agcagattac
     5881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac cgcgatcccc
     5941 gtcagttcag aagacgaaga tggtgcaacg gttcctcctt gtacatcacg aatatagttt
     6001 gcttgacatc ctgacaagaa taatattgta attagaaaaa ttattatttt catatttctt
     6061 tccaacaaaa gtaaaaatac ttatgcattt aaaatactac ctacataatt tacctgaatc
     6121 acttatacca tggattctta ttttaatatt taacgacaat gataacactc ctttgttatt
     6181 tatatttata tcatcaatac atgtattgct atatccatac tctaaattaa ccatatctag
     6241 atatatcaaa gaaaatacaa agttaaaaaa agaaccctgg tacttatgca agttatctgc
     6301 attgttttat ttattaatgg caatcccagt aggattgcca agtttcatat attacactct
     6361 aaagagaaat taaatcccta acaactcatt aagtttgctc aactcatcat cccctatgta
     6421 agaagaaaca atacctgtga caattgcaat cccccagaaa ccaagaggca caccaacaat
     6481 aaaactaaac attaatgcca caatttttgc cacacctaca tcgaccgcac ttttctccag
     6541 cgtgacaaac aaaggtcgcc agttaccagt ttctattgca tttttaaaat cagaaccaac
     6601 atcatataga aaagatactc gactggtgat tttaagcgac tttgatatct tcgttaaatt
     6661 cttagataac tcatcataat taacagactc taacgcatta aaaatagcat cccgatcaac
     6721 cttactgaat ttcttatcca gtacattctt atacttttca aatgctgcca gagcttcatc
     6781 aacaccttga attttctttc ctttagactt ttccgctaaa tcctgagcaa tttttgcgta
     6841 tttttctcca tattgttcgg ttatatattg ataaaatcca accatagtat caacagcatc
     6901 ttttatctgg ctcttttcaa gagcatcctg agcttctttt aatttctgct ctgcctcttt
     6961 aacctcagcg gccttaccat ccctgaaact aacag
//
