LOCUS       NZ_CP145692             6995 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-KEN-04-A plasmid p2-S1-KEN-04-A, complete
            sequence.
ACCESSION   NZ_CP145692
VERSION     NZ_CP145692.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052297
            Assembly: GCF_036884645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 339.8424X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884645.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:16:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,154
            CDSs (total)                      :: 5,034
            Genes (coding)                    :: 4,844
            CDSs (with protein)               :: 4,844
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 66 of 190
            Pseudo Genes (incomplete)         :: 124 of 190
            Pseudo Genes (internal stop)      :: 40 of 190
            Pseudo Genes (multiple problems)  :: 40 of 190
            Pseudo Genes (short protein)      :: 4 of 190
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145692.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-KEN-04-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2-S1-KEN-04-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            join(6775..6995,1..13)
                     /locus_tag="V6S30_RS00735"
                     /old_locus_tag="V6S30_00735"
     CDS             join(6775..6995,1..13)
                     /locus_tag="V6S30_RS00735"
                     /old_locus_tag="V6S30_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001554706.1"
                     /translation="MTELEMHLLNALEQLQQDYMQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(497..919)
                     /locus_tag="V6S30_RS00740"
                     /old_locus_tag="V6S30_00740"
     CDS             complement(497..919)
                     /locus_tag="V6S30_RS00740"
                     /old_locus_tag="V6S30_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1041..1319
                     /locus_tag="V6S30_RS00745"
                     /old_locus_tag="V6S30_00745"
     CDS             1041..1319
                     /locus_tag="V6S30_RS00745"
                     /old_locus_tag="V6S30_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1311..1601)
                     /locus_tag="V6S30_RS00750"
                     /old_locus_tag="V6S30_00750"
     CDS             complement(1311..1601)
                     /locus_tag="V6S30_RS00750"
                     /old_locus_tag="V6S30_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1842..3494
                     /gene="cka"
                     /locus_tag="V6S30_RS00755"
                     /old_locus_tag="V6S30_00755"
     CDS             1842..3494
                     /gene="cka"
                     /locus_tag="V6S30_RS00755"
                     /old_locus_tag="V6S30_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_001554611.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSNSGVHWGGGSG
                     HGNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVSNVINEK
                     QKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNS
                     YKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAE
                     SDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENEL
                     KNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIA
                     QDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTK
                     ISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVG
                     VPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(3491..3781)
                     /gene="cki"
                     /locus_tag="V6S30_RS00760"
                     /old_locus_tag="V6S30_00760"
     CDS             complement(3491..3781)
                     /gene="cki"
                     /locus_tag="V6S30_RS00760"
                     /old_locus_tag="V6S30_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            3811..3942
                     /locus_tag="V6S30_RS00765"
                     /old_locus_tag="V6S30_00765"
     CDS             3811..3942
                     /locus_tag="V6S30_RS00765"
                     /old_locus_tag="V6S30_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4137..4280)
                     /locus_tag="V6S30_RS00770"
                     /old_locus_tag="V6S30_00770"
     CDS             complement(4137..4280)
                     /locus_tag="V6S30_RS00770"
                     /old_locus_tag="V6S30_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(4948..5139)
                     /locus_tag="V6S30_RS00775"
                     /old_locus_tag="V6S30_00775"
     CDS             complement(4948..5139)
                     /locus_tag="V6S30_RS00775"
                     /old_locus_tag="V6S30_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001554612.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDNGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5207..5530
                     /locus_tag="V6S30_RS00780"
                     /old_locus_tag="V6S30_00780"
     CDS             5207..5530
                     /locus_tag="V6S30_RS00780"
                     /old_locus_tag="V6S30_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5739..>6173
                     /locus_tag="V6S30_RS00785"
                     /old_locus_tag="V6S30_00785"
                     /pseudo=""
     CDS             5739..>6173
                     /locus_tag="V6S30_RS00785"
                     /old_locus_tag="V6S30_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6208..6768
                     /locus_tag="V6S30_RS00790"
                     /old_locus_tag="V6S30_00790"
     CDS             6208..6768
                     /locus_tag="V6S30_RS00790"
                     /old_locus_tag="V6S30_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          4776..4863
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4209..4765)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5521..6995
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.593"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gaacagcaga taagggatga acaggaaagg caggcacgcc tggaacggga gcgtgcgcgg
       61 tcctggcatg aagagcagga acgggaagca cgggtatggc gtggcccgtc aataggtatg
      121 tgacggtgag ttgttccggg aggttgccag atggtaccgg aacgcgttaa aaacgcatcc
      181 gggcgcgatt ctggcgtgtc gtttttgttg ctgtgttttg cctctttgcc agatcgcgct
      241 cattcctcag cctcatcccg cgatgtctgc gtatgtcatc aactgaaaag atggaaaaaa
      301 aacagtgtca tgaggtttac gcaggcaaaa ccagcgttat tcacatggct gaatagctat
      361 gcatacggtt aaaatttatc aggtgcgatc gcggcagctt ttcgggtggt ttgttgccat
      421 ttttacctgt cagcagccgt gatcgcgctg aacgcatttc agcggtgcgt acaattaagg
      481 gattatggta aatcccttac tgtctgccct cgtatccatc gagataaacc gcagaaatgg
      541 tgtctgccat tagtttcatt tttttcggcg aaggcatatc gctgtttgtg tattttctgc
      601 cagcaaaggc tgttgtgctc cggagtgagt gaaaaaattt ttctgagtgg aattctttta
      661 cacgttgctg tgccgtttct gctgatactc cggctgcttt gtctttcatc cccatatccc
      721 ggaactgctg aaagaacggg agcatcatac gtatggtgtc tgctttctgg gtgtagaagt
      781 taggatcaaa tccatcatct gaagcataaa cataatgccg ggtatgtcgt tccaggctgg
      841 tctttggtgt gcatcctgta agagctattg ttgctgttat cagaggtatc agaagcagcc
      901 tggcagcggc agatctcata aacatctcct tgcgttttta atcttgatac tcagggagtt
      961 atatctccga agagtatctc ttatcatacg tcggtttact cccaatgtga tttgatggtc
     1021 aatgagtcag gaataatccc gtgaaaaaat gtgtgccagt cctgcttttt agtgttcttt
     1081 ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc gagaagacaa
     1141 aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaat agactgaaga
     1201 tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat gcccaaaaaa
     1261 gagaggattt gcgtaaaaaa ctggagtctc tggagcggca aaagaaatcc ttacgctgat
     1321 gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata tttacgggcg
     1381 aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt cacaccatta
     1441 ctgatttcac tgaccacatg acgctgatca gccagtgtca gcgtgtaaac ctgtttctgg
     1501 atgaggtcac gggctttgtc atgctcatat ccggcagcaa caagggtgtc gagaagtcgt
     1561 ttgtctgttt tgttcagttt atggcggggt gatttgctca catcgttctc cgttttttca
     1621 tgatccaaca gttttatatc tcatcctgat ttgctccggt gttctgtttc gacgttctca
     1681 atgcctgcct ccccatagtg tgatccttat tgcaaaaatg aaaatccatg ctcttgacat
     1741 ggacaatgct gagtagtagg tttttactgt acataaaacc agtggttata tgtacagtat
     1801 attgttttta atttattgtt ttaattatca aagaggaatt tatggctaaa gaactaagtg
     1861 gatatggacc aactgctggt gagtctatgg ggggaacagg ggcgaatctg aatcagcaag
     1921 gtggaaataa taatagtaat agtaatagtg gggttcactg gggtggtggc tctgggcatg
     1981 gtaataatgg tggtcaggga aattcaaatt catctggttc tacatcaact gtgatgaaaa
     2041 ccggtgagtc atatctgact ccgtggggtg atgttgtcat taataatgat ggccttccgg
     2101 tgatgaatgg tattgttatg acagaagaga attcaactct ggttgataat ccatttggag
     2161 gggtctccag agttcttaat tctttaatta gtgatatgcc atcacttttt gcagaaagct
     2221 caggaaataa caacaataac actgcttcag ttaatactgc accaacgaat gctcaggtca
     2281 gtgatatgga taagagttcg aaggtggtga gtaatgtaat taatgaaaaa caaaagcaga
     2341 agaataaaat tgctacacag attagtgaaa aacagaagaa aatcgaggag atgaaaaaag
     2401 tattcaagca tcattcttat catggtatca ctgatcttga gagggatgtt gatgagttgc
     2461 aaaaaaaatc aaatcagctt gatgcagata ttagcaaact taattcttat aaaaatacat
     2521 tgcaaagtaa aattggtgat gtgaataaac agaaggaagc tgaagaaaag gctcgtgaaa
     2581 atgcagaggt cgctgaacat gaaactctta atgaagagaa acaggcggtg gcggaagcag
     2641 agaagcgttt agctgaggcg aaagctgaac tggcgaaggc tgaaagtgat gtacagagta
     2701 agcaagctac tgtttccaga gttgcaggag agcttgaaaa cgctcagaag agtgttgacg
     2761 tgaaggtcac cggatttccg ggatggcgtg acgttcagaa aaaactacag cgtcagcttg
     2821 aagctaagca ggctgagtac tccgcagttg aaaatgaact taagaatgct gttagtttca
     2881 gggatggtaa ggccgctgag gttaaagagg cagagcagaa attaaaagaa gctcaggatg
     2941 ctcttgaaaa gagccagata aaagatgctg ttgatactat ggttggattt tatcaatata
     3001 taaccgaaca atatggagaa aaatacgcaa aaattgctca ggatttagcg gaaaagtcta
     3061 aaggaaagaa aattcaaggt gttgatgaag ctctggcagc atttgaaaag tataagaatg
     3121 tactggataa gaaattcagt aaggttgatc gggatgctat ttttaatgcg ttagagtctg
     3181 ttaattatga tgagttatct aagaatttaa cgaagatatc aaagtcgctt aaaatcacca
     3241 gtcgagtatc ttttctatat gatgttggtt ctgattttaa aaatgcaata gaaactggta
     3301 actggcgacc tttgtttgtc acgctggaga aaagtgcggt cgatgtaggt gtggcaaaaa
     3361 ttgtggcatt aatgtttagt tttattgttg gtgtgcctct tggtttctgg gggattgcaa
     3421 ttgtcacagg tattgtttct tcttacatag gggatgatga gttgagcaaa cttaatgagt
     3481 tgttagggat ttaatttctc tttagagtgt aatatatgaa acttggcaat cctactggga
     3541 ttgccattaa taaataaaac aatgcagata acttgcataa gtaccagggt tcttttttta
     3601 actttgtatt ttctttgata tatctagata tggttaattt agagtatgga tatagcaata
     3661 catgtattga tgatataaat ataaataaca aaggagtgtt atcattgtcg ttaaatatta
     3721 aaataagaat ccatggtata agtgattcag gtaaattatg taggtagtat tttaaatgca
     3781 taagtatttt tacttttgtt ggaaagaaat atgaaaataa taatttttct aattacaata
     3841 ttattcttgt caggatgtca agcaaactat attcgtgatg tacaaggagg aaccgttgca
     3901 ccatcttcgt cttctgaact gacggggatc gcggttcagt agaaaagatc aaaggatctt
     3961 cttgagatcc tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa ccgcctgggg
     4021 aggcggttta gtcgaaggtt aagtcagttg gggactgctt aaccgggtaa ctggctttag
     4081 tggagcacag ataccaaatg ctgtccttcc agtgtagccg ctataaggct tcaacttcaa
     4141 gagttctgcg tatctaaatc cactatccag ttaccaatgg ctgctgccag tggcgttaag
     4201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4321 taccaacagc gtgagctatg agaaagcgcc acgcttccca aagggagaaa ggcggacagg
     4381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagccacc agggggaaac
     4441 gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt cggtttttgt
     4501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag ctgcgtggcc ttttccgggt
     4561 tcttgttttt tgctcacatg tttcttttgg tgttatcccc tgattctgtg gataaccgca
     4621 ttaccgtctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cggagcgagt
     4681 cagtgagcga ggaagcggaa aagcgcctgg acgagcattt tctccttacg catctgtgcg
     4741 gcatttcaca cccggcatgg tgcacttttc atacaatcct cactgatgcc aaatgattaa
     4801 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgct
     4861 aaaacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc acaggccagc
     4921 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgttatccc cgaagcgtgc
     4981 gaggcagctg cggtaaagct cgtcagcgtg atcgtgaagc gcttcgcaga tgtcagcctg
     5041 gtcgtcggcg tccagttcat tcagtttttc cagcagggtc agcgtctggc ttctgatgaa
     5101 gcgggccatg ttaagggcgg ttttttcctg tttagtcaca gttctgtttc tccatgtgtg
     5161 gggagtctgt cggaggtttt taatgatacc ggtgaaacgc gaaaggatgc tcacgatacg
     5221 ggtcacggat gaggaacatg cgcggctgct ggcacgttgt gagggaaaac aacttgcgtc
     5281 atggatgcgt aaggtctgcc tgggggcacc gccatcgaaa acgtcaggtc ttccgacact
     5341 ggcaccgccg ctgttgcgcc agttcgcttc ggtcggcaat aacctgaatc agatagcccg
     5401 gaagataaac agcggacagt ggtcggggca tgaccgggtt catgtggtgg ctgcactgat
     5461 ggccatcgga cgtgaactga gtgagctgag ggatgaggtc cgtaaacagg gagaacggga
     5521 tgatagttaa gtttcatgcc aggggaaaag gcggtggcag tggtccggtt gattacctgc
     5581 tgggccggga gcgtaaccgg gaaggggcga gagttctgcg gggtgctccc gaagaggtgc
     5641 gggaactcat cgatgccacg ccgtttgcga aaaaatacac ctcgggggtt ctgtcttttg
     5701 cagagcagac cctgccaccg ggagagcgtg aaagggtgat ggagagcttt gagcgggtac
     5761 tgatgcccgg tcttgaaaaa aatcagtaca gcatcctgtg ggtggagcac caggacaagg
     5821 ggcgtcttga gctgaatttt gtcatcccga atatggagct ggcgagcgga aaacggctcc
     5881 agccctatta cgaccgagcg gaccgtccgc gaatcaatgc ctggcagaca ctggtgaatc
     5941 accattacgg gctgcacgac ccgaacgcac cggagaacag gcggacactt gtcaccccga
     6001 ataatctgcc gaaagcgaaa caggaggccg cagaagcgat tacgagggga ctggaggctc
     6061 tttaccatgc cggagcgcta aaaacgcgtc aggatgtcac tgaggcgctt acagcggcag
     6121 gttttgaggt tgtgaggaca accaggagca gtatcagtat cgccgacccg gagggtgggc
     6181 gtaatctcag gctgaaaggg gcgctgtatg agcaatcttt tacaaatggg gacagacttc
     6241 gagaagaaac tgaaagagcg tgccgtctct acagagaaca tgctgaacag cgagttcagg
     6301 aagctcgaag cgtctgtaga cagggaactg aacttaaacg ggcagaaaat cagagacgct
     6361 atcagcgcac acacagcagc ggtgaaagaa caactggaga cgctgaacag tgccgtgaac
     6421 gcgcagttca gcgccacaga gaacgaactg gcccggcggc aggaagagct gctctggaag
     6481 ctggtcaaag ggcggatact gtacccgtcg ctgacggcgc tttgcgtcac gggcgggatt
     6541 tttctggcgt catgggggct gattcagtgg caggaaagcc ggattgcggc gaacatcctg
     6601 gctatcaggg accaggaaga gacgctggcg aaactccggg aaaaaacatg gggtgtgacg
     6661 tatcacgagg gcaggaacgg gaaatttcta gtgttgccgt ccggggtgaa gggggaaaac
     6721 aactggacgg tggagaagaa gaacgcagta agactggtca gggagtaagt gagtatgaca
     6781 gagctggaaa tgcacttact gaacgcctta gagcaattgc agcaggacta tatgcagcgg
     6841 ctgaaagact gggagagcgc cttcacggat ttgcagcata tgttcgagct gacacgacag
     6901 gagaacgggc aactgaggga acagtgcgac gacttgagtc agcaggtgca gagattagcc
     6961 gggcaggtgg accacttgag tcggttattt atcac
//
