LOCUS       NZ_CP145688             2101 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-KEN-07-A plasmid p5-S1-KEN-07-A, complete
            sequence.
ACCESSION   NZ_CP145688
VERSION     NZ_CP145688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052300
            Assembly: GCF_036884625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.6525X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884625.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:24:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,725
            CDSs (with protein)               :: 4,725
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 89 of 235
            Pseudo Genes (incomplete)         :: 158 of 235
            Pseudo Genes (internal stop)      :: 43 of 235
            Pseudo Genes (multiple problems)  :: 52 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145688.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-KEN-07-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5-S1-KEN-07-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            453..1418
                     /locus_tag="V6S32_RS25385"
                     /old_locus_tag="V6S32_25390"
     CDS             453..1418
                     /locus_tag="V6S32_RS25385"
                     /old_locus_tag="V6S32_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1422..1688
                     /locus_tag="V6S32_RS25390"
                     /old_locus_tag="V6S32_25395"
     CDS             1422..1688
                     /locus_tag="V6S32_RS25390"
                     /old_locus_tag="V6S32_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          847..1078
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag acagggtttt
       61 gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa gccgttcggg
      121 ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc gcgcgtgatc
      181 cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggtttag cccagtaatg
      241 acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg aggcgttttt
      301 aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca cctcagcggc
      361 aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata taccacatca
      421 aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc ctttcggact
      481 acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct gtcgggggta
      541 tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc tgtagcggtt
      601 tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta cggcttcgta
      661 gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga accctcatgt
      721 ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg tcttcccgat
      781 ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga acagtcctta
      841 cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg
      901 ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc
      961 actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt aagcacgaac
     1021 gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat atcgatattc
     1081 gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg
     1141 cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaac gatcctgagt
     1201 ggtttcttga gctgacgcgg cagcttcaca agcgccgttt tatctcgacc ggtggggcgc
     1261 taaaaaacgt cctccagttg gatcgagaaa ccaatgagga tcttgtcatt gccgacgatg
     1321 taggggatgg cactgatgac gggaagcgga cggcgtttgt ctgggattca ggtaaacggc
     1381 gttacaaacg cgcccctgag aaggataaat cggattaacg tatgaatatt aatattgaat
     1441 acctgaatgg aaataagact attggtttat tttttttaag aagtgaagcg gtgattcctg
     1501 acaggtttaa aaaccttatt ttgcttattg atggattaag ttttggcaca tttggttttc
     1561 atccgcacga aggttttgag gatgaattaa ttttatatat tcagaaaaca aacgagaggg
     1621 taaaaactct ttttgtgaaa atagatttaa acggtatcaa gtttggattt ttaagaacgc
     1681 attcttagtt ctggaaaaga gccagcggca ggctgaggtg ataggtacga gattgcatgc
     1741 aatctctagt gctctgtcta tcctgcatta tcctcagcat tatcctcagc attatcctca
     1801 gccttgccaa ctcgacacca atgcaggata gacaatccga tgtcaaatgt taacactctg
     1861 cgagtggtac attttccccg gattatcgtc ctgagcctgc cgctggctct ctttctaccg
     1921 cctcgctttg ctcgttgctc aacgcctcac agacacggat taaaatccgc atccgttcac
     1981 cgttttttaa agtccgttaa aagcatgatg ccatctccga gagttaatct cgtcaaatgc
     2041 taaatcgtgg gggtcccctt tggggttccg atttagtgat tgacgacacc accgattaaa
     2101 a
//
