LOCUS       NZ_CP145685            10850 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-KEN-07-A plasmid p2-S1-KEN-07-A, complete
            sequence.
ACCESSION   NZ_CP145685
VERSION     NZ_CP145685.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052300
            Assembly: GCF_036884625.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.6525X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884625.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:24:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,725
            CDSs (with protein)               :: 4,725
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 89 of 235
            Pseudo Genes (incomplete)         :: 158 of 235
            Pseudo Genes (internal stop)      :: 43 of 235
            Pseudo Genes (multiple problems)  :: 52 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145685.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-KEN-07-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2-S1-KEN-07-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            complement(724..1590)
                     /locus_tag="V6S32_RS25260"
                     /old_locus_tag="V6S32_25265"
     CDS             complement(724..1590)
                     /locus_tag="V6S32_RS25260"
                     /old_locus_tag="V6S32_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1592..2644)
                     /locus_tag="V6S32_RS25265"
                     /old_locus_tag="V6S32_25270"
     CDS             complement(1592..2644)
                     /locus_tag="V6S32_RS25265"
                     /old_locus_tag="V6S32_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273655007.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASRKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(3125..3358)
                     /locus_tag="V6S32_RS25270"
                     /old_locus_tag="V6S32_25275"
     CDS             complement(3125..3358)
                     /locus_tag="V6S32_RS25270"
                     /old_locus_tag="V6S32_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3365..3895)
                     /locus_tag="V6S32_RS25275"
                     /old_locus_tag="V6S32_25280"
     CDS             complement(3365..3895)
                     /locus_tag="V6S32_RS25275"
                     /old_locus_tag="V6S32_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3933..4631)
                     /locus_tag="V6S32_RS25280"
                     /old_locus_tag="V6S32_25285"
                     /pseudo=""
     CDS             complement(<3933..4631)
                     /locus_tag="V6S32_RS25280"
                     /old_locus_tag="V6S32_25285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4573..4896)
                     /locus_tag="V6S32_RS25285"
                     /old_locus_tag="V6S32_25290"
     CDS             complement(4573..4896)
                     /locus_tag="V6S32_RS25285"
                     /old_locus_tag="V6S32_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4961..5152
                     /locus_tag="V6S32_RS25290"
                     /old_locus_tag="V6S32_25295"
     CDS             4961..5152
                     /locus_tag="V6S32_RS25290"
                     /old_locus_tag="V6S32_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5820..5933
                     /locus_tag="V6S32_RS25295"
                     /old_locus_tag="V6S32_25300"
     CDS             5820..5933
                     /locus_tag="V6S32_RS25295"
                     /old_locus_tag="V6S32_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            6339..6728
                     /locus_tag="V6S32_RS25300"
                     /old_locus_tag="V6S32_25305"
     CDS             6339..6728
                     /locus_tag="V6S32_RS25300"
                     /old_locus_tag="V6S32_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(6792..7076)
                     /locus_tag="V6S32_RS25305"
                     /old_locus_tag="V6S32_25310"
     CDS             complement(6792..7076)
                     /locus_tag="V6S32_RS25305"
                     /old_locus_tag="V6S32_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(7094..7291)
                     /locus_tag="V6S32_RS25310"
                     /old_locus_tag="V6S32_25315"
     CDS             complement(7094..7291)
                     /locus_tag="V6S32_RS25310"
                     /old_locus_tag="V6S32_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            7503..7799
                     /locus_tag="V6S32_RS25315"
                     /old_locus_tag="V6S32_25320"
     CDS             7503..7799
                     /locus_tag="V6S32_RS25315"
                     /old_locus_tag="V6S32_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            complement(8344..8577)
                     /locus_tag="V6S32_RS25320"
                     /old_locus_tag="V6S32_25325"
     CDS             complement(8344..8577)
                     /locus_tag="V6S32_RS25320"
                     /old_locus_tag="V6S32_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8584..9114)
                     /locus_tag="V6S32_RS25325"
                     /old_locus_tag="V6S32_25330"
     CDS             complement(8584..9114)
                     /locus_tag="V6S32_RS25325"
                     /old_locus_tag="V6S32_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015386306.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<9152..9850)
                     /locus_tag="V6S32_RS25330"
                     /old_locus_tag="V6S32_25335"
                     /pseudo=""
     CDS             complement(<9152..9850)
                     /locus_tag="V6S32_RS25330"
                     /old_locus_tag="V6S32_25335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9792..10115)
                     /locus_tag="V6S32_RS25335"
                     /old_locus_tag="V6S32_25340"
     CDS             complement(9792..10115)
                     /locus_tag="V6S32_RS25335"
                     /old_locus_tag="V6S32_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_046072959.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLCQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            10180..10371
                     /locus_tag="V6S32_RS25340"
                     /old_locus_tag="V6S32_25345"
     CDS             10180..10371
                     /locus_tag="V6S32_RS25340"
                     /old_locus_tag="V6S32_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5238..5324)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.47e-38"
     Typing          complement(10466..10547)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(154..341)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          5336..5892
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.642"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10579..10828
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.304"
                     /coverage="100.0"
                     /evalue="6.85e-92"
     Typing          complement(3022..4583)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.808"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8241..9802)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.616"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgggagcct tgcggctcaa ccatgaaatc cgccagcggt ggcgaaaccc gacgtggctt
       61 aaagatcatc accgtttccg gtgaatcgct ccctcctgcg cccccacgtt cccgccatat
      121 cgattaccgg ataccttttc cgcctttctc ccttacggga cacgtggcgc tttctcatag
      181 ctcacgtctt ctggtatctc ggggcgactt aggcagttcg ctccgggctg ggctgtatgc
      241 aggaaccccc cgttcagccc gactgctgcg ccttctccgg taactgttct cttgggtcca
      301 acccggaaaa gacaccacaa agcgccactg gcagcagcca ttggtaaaag tcatgtgcga
      361 gatatttaga gagtacttga agtggctgcc ggagtgcggc tacactagaa gaacaatttt
      421 ggtgactcgt ctcgcataag acagttacct ggttaagcag tctgcaagct gacttaacct
      481 tcgatcaaac cgccctgcaa ggcggttttt tcgtttacag gcagcagatt acgcgcagaa
      541 aaaaaggatc tcaagaggat cctttgatct ttttgttaca aacgtctcta tttgactccc
      601 ctgagagcgt tttagcgcgc ctctgacccc cttggttaca ttccgacacc agaaacgctc
      661 ccgtttttta cgtgcatttg gcacggtatc gatggaggcc ggggggcaat cctgaacaga
      721 atgttactcc tctgagtatt ttctgttaag aaaatataca cttagcacag ttacaataaa
      781 tcctaccccc tgtaagaatg tgaattgttc atccaaaaac aaccatgaca aaaataatgc
      841 aactagcgga ataaagtaca acattatccc tgccaaatga actgggagcc aacgcaagga
      901 gttcaagtaa aaacaaaaag ctaaaaaata ttgtaatatt ccagatgaaa aaacaataat
      961 cacggattcc aacggaagat aaattgatga tttagatata aaaataaaga gcgatgacag
     1021 tataagggag aatgtcaact gtccgctcaa taatgtgata ggctcaaacg atttaataag
     1081 gcgagacgat ataacaacat aaaatgctgc cgataacaca ccggcaacaa caagacaata
     1141 tccaacaata ttattcgatc cattactgat gtctggcaag gctaccaaaa tactgccaac
     1201 aattgcacct ataaggaaca atataaaata ttgcggggaa ttaattttaa ctgagcaaat
     1261 aagaacagac aacaccaata taaacactga ttcaagtgaa aaaataattg atgcgcttcc
     1321 tgctgaaata aaataaagtc cgtagagtgt aaaagtatac gctaagaatg gttcaaaaac
     1381 gcctgtcgca taagcaagta tcgcttttga gtttaatggg agtttttttc tttttataaa
     1441 aagaactatc caagatagta ttactgcact acaaatctgc cagaaaaaca cccctccggc
     1501 atctagttct tcaactcctt ttttgctcat tacgatagct gccccccatg agaacgctgc
     1561 taaaaaagca aaaagcattc ctttaatcat attaatcatc ctctttctca taaaggggtt
     1621 tgttttcaaa tttccttggt tgaaaggagt taaaaaacat agatgcatca tctacaggaa
     1681 taccaataaa ctctccataa taaggatcat ctataggaaa ctcttctaat gaaacataaa
     1741 acaatacatt ccagttgaag atcattggat cactattttc tatttcatgg agcaatcttt
     1801 catccttagt taattctcct ggaattaata catcagtatg ataaaaaccg ctgtaataag
     1861 aactattacc ggtgtgcact gggcatccaa aacgatccaa attagattct gaatgccatg
     1921 ttgtagaaat cccttccgga tgtgaatacc cacccttacc aacagactga atagtcatgt
     1981 tacgaatatc cgctaacgct tgttcttgat ctttatagaa acctttttct atcgccagcc
     2041 tcataaaatg ccccggggca tttacagaaa taagtattcc ccctggtatt cttctatcat
     2101 gatttcttgt tctatgagca gaagagtaaa aaacattaac accagcctta tatattttta
     2161 gcccatcttt atcctgaaat ggtacgcttt cagtataaat aacatcctct tttattggat
     2221 agcactctgg catatgcagc gaacaaatat atttttgtat gtccactaac tcttctgatg
     2281 gacgagcaaa ggcaagttgt ggatgaataa aataaatgag atgcgcactg acctctcctc
     2341 ttcttgcgcg ttctttaaaa cgaactcttt cattacgcaa aaaatcaaac aaatgaccat
     2401 catcattata tagttggggg ctatttacaa tagataaatg gaaatcaagc cctttaattt
     2461 tcctgcttgc caatttacca aatacacagg gctgattgcc tgacacccat tttcttagta
     2521 aatcttcaat tttatcttta gaagtttcgt taaagataaa tgaatgaata ttatcaatat
     2581 cttttccaaa tgaaaaactg gatgataatt gtggtagttt atttatacgt tgttgaagat
     2641 tcatgaaagc tcccacaaga cacctcatat atttttttat atactgaaac tacattatat
     2701 ttattaccgg cgtaaaacga aataatttat tatcgttacc gatattttaa aaaaatcatt
     2761 atttgcacag tgttcgagtt gttagtatca ctccttcctg aaaatccgct ccatttacca
     2821 taacgtcgca ttgtacgcac cgccaaaacg cgttcggtgc gatcacggca gcagacaggt
     2881 aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc acctgataaa ttttaaccgc
     2941 atgaatacct atgcaaccag atgctacagg ccacattagc ccgccttaat ccactgaaca
     3001 cgtcattttt catggtttca ccatcccagc gaagggccat ccagtgtccg ctcctggatt
     3061 tccggctgac gctcccgctc cagagataac tcatgttcgc gttcctgcat cagccgttca
     3121 tcccttatct cctgttctcg ctgtataact ggctcaagcg ttctgtccgc tcgctcaagt
     3181 gctgcacctg ctgactcaac tgcatgactc gcccgttcag catcgcgttg tcccgttgcg
     3241 taagcgaaaa cattttctgc aattccacga aggcgttctc ccattcgttc agccgctgca
     3301 tatagtcctg ttgcagttgc tctaatgcgt tcagcaaatg tctttccagc tccgtcatgc
     3361 tctgtcactc ctgcaccagt ctcactgcgt tcagtttgcc gttgtccttc gtccagttcg
     3421 tttcggcttt catccccttc ggcaacacca gaaaacgtcc gttgctgtcc tcgtgataac
     3481 gcacgcccca tgtcttcgcg ttcagcttct ccagactctc tttctgctcg cggagaatat
     3541 tcatattcgc gtttatcttc atgccctggt accacagcag actcccggtc gtggcaaaca
     3601 tcagtactga gacgataatc agccacagcc agcgtttcac gctcattttc agtacggttt
     3661 tccagtgcgc gtccatcgta tcgacggtcg ttttctgata cctctccagc agttctttca
     3721 gggctgttgt atggtcgttg atagcgttcc tgattaaaga ttcgtttgag gtcagttccc
     3781 tgtttacaga ttcttcgagc ttcctgaact cgtcgttcag cattttctct gtagattcgg
     3841 ctctctcttt cagctttttc tcgaactccg cgcccgtctg taaaagattg ctcataaaac
     3901 gctcctttca gtctgatatt cctcccgccg ttcgggtctg caatgctgat actgcttcgc
     3961 gtcaccctga ccacttcaag ccccgcctct gtgagcgcct gaatcacatc ctgacggcct
     4021 tttatctctc cggcatggta aagcgcgtct atcccgcgtg tgacgccctc agcaagcgcc
     4081 tgtttcgttt ccggcaggtt atcaggaagg gtcagcgtcc ggcggttctc cggggcgttg
     4141 gggtcatgca gaccgtaatg gtgattcacc agcgtctgcc aggcatcaat tctcggcctg
     4201 tctgcccggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg
     4261 acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtgctga
     4321 tttttttcga gaccgggcat cagtacacgc tcaaagctcg ccatcacctt ttcacgtcct
     4381 cccggtggca gctccttctc cgcgaacgac agcacaccgg acgtgtattt cttcgcaaat
     4441 ggcgtggcat cgatgagttc ccgtacttct tccggattac cctgaagcac cgttgcccct
     4501 tcccggttac gctcccttcc cagcaggtaa tcaaccggac cactgccacc accttttccc
     4561 cgggcatgaa acttaactat catcccgcgt cccctgttcc ctgacggcca gacgcagccg
     4621 gcgcagctca tccccgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga
     4681 ccactgcccg ctgttcacct tcctcgctgt ctgattcagg ttattcccga tggcggccag
     4741 ctgacgcagt aacggcggtg ccagtgtcgg cagcctcccg gaacgggcaa ccggctcacc
     4801 caggcagacc cgccgcatcc ataccgccag ctgtttaccc tcacagcgtt caagtaaccg
     4861 ggcatgttca tcatcagtaa cccgtattgt gagcatcctc tcgcgtttca tcggtatcat
     4921 tatccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac
     4981 cgcccttaac atggcccgct ttataagaag ccagacatta acgctgctgg agaagctcaa
     5041 cgaactggac gcggatgaac aggcagatat ctgtgaatcg cttcacgacc acgccgatga
     5101 gctttaccgc agttgcctcg ctcgcttcgg ggatgacggt gaaaacctct gacacatgca
     5161 gctcccggag acggtcacag cttgtctgtg agcggatgcc gggagctgac aagcccgtca
     5221 gggcacgtca gcgggtttta gcgggtgtcg gggcgcagcc ctgacccagt cacgtagcga
     5281 tagcggagtg tatactggct taaccatgcg gcatcagtgc ggatggtatg aaaagtgcgc
     5341 cataccgggt gtgaaatgcc gcacagatgc gtaaggagaa aatgcacgtc cagccgcttt
     5401 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggtatct
     5461 actcactcaa aaacgaaatg ctgttatcca cagaatcagg ggataacacc ggaaaaaaca
     5521 tgtgagcaaa agaccaggaa caggaagaag gccgcgccgc tggcgttttt ccataggctc
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5641 ggacttaaag ataccaggcg tttccccccg gaagctccct cgtgcgctct cctgttccga
     5701 ccttaccgtt taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5881 ccaacccggt aagacacgac ttaacgccac tggcagcagc cattggtaac taaaaagtgg
     5941 atttagatac gcagaactct tgaagttgaa gccttatagc ggctacactg aaaggacatc
     6001 ttttggtatc tgtgctccac ttaagccagc tacccggtta ggtaatctgc caggattagc
     6061 taaccttcga aaaaccacct gccagggtgg ttttttcgtt tacaggcagc agattacgcg
     6121 cagaaaaaaa ggatctcaag aagatccttt gatctttttt ctctaaactg caatcccatc
     6181 agttcaggag agaaagatag tataattgcc ctctcttata tggaaccacc attcaatcac
     6241 atcatgggaa taatataagt gaaaacgttc gcattgttta tttttacgtg tatatgtatt
     6301 tatagattgg aattttaaca aaataaaatg gagttaatat gttcaaatca tcagtaaagt
     6361 caataacatc gagtactata ctgaccttac ttataggatg ttcaagtact gaaacaaaca
     6421 aaaacattgt ttacaaagtt ggtggtgaca gcatcattct gaataaatat tgcatactta
     6481 aaatgtaccc ttcccaatca gaaaaaaaca aaggcgtcat aatagaagta aacacagata
     6541 gtttatgtaa gaaaaaacta aacgaaataa ctagtaaaaa tatagggaaa cccctgattt
     6601 catattttga tggaaaagta atatcccaac ctgcaatcat tcattctgca ctaaatccaa
     6661 cccattatta tcaacctgtg aacgatgaaa caacagtgat ggaaatcata aaagcatatt
     6721 caaaataata ttaatagata taatcctgct taaggtatag cttaggcagg attatatcta
     6781 ttcagctctt attataaggt agtgtataca tgaataatct gccctgtccc actgacactt
     6841 ttaaatttaa ttttatcaac accagcgccc ttgcaagaga tctgataaga tgctggacca
     6901 ataacgttaa ctgttcttgg cccattaggg gcataccaga taaaagcaat aaatggaccg
     6961 ggtacattag acaatgtatc aaaatagaca gtgtacgcga tatcaccagt atgattttca
     7021 agttcaaccc aaggaccaaa aaagaaagga ggagcccaaa cattttgaga aatcatataa
     7081 tctcctgatt agttcaatgc gaatgttctt cccattcact ctcgcaagaa acatctataa
     7141 gctcaacatc tagcccacta acagaatcag agaaatactt ataaacatca tcccttgttc
     7201 ccataaggtc tgccgaacat gatagttcac aaccattctt attcctaact acaaataaga
     7261 aacggtgaag caatactttt tcttctttca ttttatccct ccctttattt acacagaata
     7321 tactgtatat ataaacacta gttttatata cagtataaat tacgccgcat tatttttcct
     7381 gtcaatgata tgaattttca tttttgtgat ctgaatcacc cttataaaaa tcaggaaggg
     7441 aagattcgca gcagaaaaac agcaccggat aacatcagga gaaaaaacag agaggagata
     7501 acgtgagcaa aacaaaatct ggtcgccacc gactgagcaa aacagacaaa cgcctgctgg
     7561 ctgcacttat cgctgccgga tacgaagagc ggacagcccg tgacctcatc cagaaacacg
     7621 tttacacact gacacaggcc gacctgcgcc atctggtcag tgaaatcagt aacggtgtgg
     7681 gacagtcaca ggcctacgat gcgatttacc aggcaagacg cattcgtctc gcccgtaaat
     7741 tcctgaacgg aaaaaaaccg gaagaagggc tgaacgcctg gaagggtgat ttaccataat
     7801 cacttattgt acgcaccgct gaaacgcgtt cggcgcgatc acggcagcag acaggtaaaa
     7861 atggcaacaa accacccgaa aaactgccgc gatcgtaccc ggtaaatttt aaccacatgc
     7921 atagctatgc agctatgtga atcatgcagg aattgccagc cagagacagc tgaaacggat
     7981 ttttttcatg ctttcggaag cgaagaagac ggggaccgga gcgggaaaac agaagttcag
     8041 gggaatgagc gcgatctggc aatagaggca aaacacagca acaaaagaca caccagaatc
     8101 gcgcccgtat gcgttttaac gcgttcctgt gttttcagca aggtctgact gaagcgcgtt
     8161 cccggtgccc ccgaaccaac cattatccca aaaaatgcac cggaaacgcg ctgacaacat
     8221 tttagcctgt ctctgattac catcccagcg aagggccatc cagtgtccgt tcctgtattt
     8281 ccagctggcg ctcacgctcc agggataacg catgttcgcg ttcctgtatc agccgttcgt
     8341 cccttatctc ctgttctcgc tgtataactg gctcaagcgt tctgtctgct cgctcaagtg
     8401 ctgcacctgc tgactcaact gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt
     8461 aagcgaaaac attttctgca attccacgaa ggcgctctcc cattcgttca gccgctgcat
     8521 atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct ccgtcatgct
     8581 ctgttactcc tgtaccagcc ttacggcatt ctgtttcccg ttttccatcg tccagttcgt
     8641 ttcggctttc atcccctcgg gcaacaccag aaaacgtccg ttgctcccct cgtgataacg
     8701 cacgccccat gtcttcgcgt tcagcttctc cagattctcg ttctgcttcc tgatttcttc
     8761 caggttcgcc tgtatcatcc cgccaagata ccagagcgtc ccgccactca cggtaaacag
     8821 gaaaatgacc atccccagta acatcatgcc cgtattccct gccagcttta acacgtcctt
     8881 cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt tttttcagtg attcctctgt
     8941 cgaagctgtg aacagggcta tagcgtcttt gattttcgtc tcgtttgatg tcacagcctt
     9001 gcttacagat ttttcgagct tcctgaactc gtcgttcagt attgtctctg tagattcggc
     9061 tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc tcataaaatg
     9121 ctcctttcag cctgatattc ttcccaccgt tcgggtctgc aatgctgata ctgcttcgcg
     9181 tcaccctgac cacttcaagc cctgcctctg tgagcgcctg aatcacatcc tgacggcctt
     9241 ttatctcccc gacatgataa agagcgtcta tcccgcgtgt gacgctctct gcaagcgcct
     9301 gtttcgtttc cggcaggtta tcagagagag tcagtatccg gcggttctcc ggggcgttgg
     9361 ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt
     9421 ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg ttcggaatga
     9481 caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg ctgtactgat
     9541 ttttctcgag accgggcatc agtacacgct caaagctcgc catcactttt tcacgccctc
     9601 caggcggcag ctccttctcc gcgaacgaca gaacacctga cgtgtatttc ttcgcaaatg
     9661 gcgtggcatc gatgagttcc cggacctctt ccggattccc ctgaagcacc gttgcccctt
     9721 cccggttacg ctcccttccc agcaggtaat caaccggacc actgccacca ccttttcccc
     9781 tggcatgaaa cttaactatc atcccgcgcc ccctgttccc tgaccgccag acgcagccgg
     9841 cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc accggaagac
     9901 cactgcccgc tgttcacctt cctcgctgtc tggttaaggt tattcccgat ggcggccagc
     9961 tgacacagta acggcggtgc cagtgtcggc agcttcccgg aacgggcaac cggctcaccc
    10021 aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc cagtaaccgg
    10081 gcatgttcat catcagtaac ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt
    10141 acccccatga acagaacccc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
    10201 gcccttaaca tggcccgctt catcagaggc cagacattaa cgctgctgga gaagctcaac
    10261 gaactggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgccgatgag
    10321 ctttaccgca gttgcctcgc acgcttcggg gatgacggtg aaaaccgctg acacatgcag
    10381 ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagcagaca agcccgccag
    10441 ggcgcgtcag gccgttgacc tttggggtgt cggggcgaag ccctgaccca gtcacgtagc
    10501 gatagcggag tgtatactgg cttaaccatt cggcatcagt gcggattgta tgaaaagtgc
    10561 gccatgtccg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgccag ttcaggcgct
    10621 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggact
    10681 ctcctttctc aaaaacgcta accctgttat ccacagaacc cggggataaa ccaagaaaga
    10741 acatgtgcgc aaaaaacgaa accccgggaa aggccgcgcc ggaggcgctt tttccatagg
    10801 ctccgccccc ctgacggact tcacaaaaca gacacttttt cggtgcatct
//
