LOCUS       NZ_CP145654             2088 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-IND-02-A plasmid p8-S1-IND-02-A, complete
            sequence.
ACCESSION   NZ_CP145654
VERSION     NZ_CP145654.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052310
            Assembly: GCF_036884425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 276.8158X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884425.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:14:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,448
            CDSs (total)                      :: 5,327
            Genes (coding)                    :: 5,106
            CDSs (with protein)               :: 5,106
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 81 of 221
            Pseudo Genes (incomplete)         :: 146 of 221
            Pseudo Genes (internal stop)      :: 40 of 221
            Pseudo Genes (multiple problems)  :: 44 of 221
            Pseudo Genes (short protein)      :: 5 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145654.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-IND-02-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p8-S1-IND-02-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2022"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            complement(join(1985..2088,1..862))
                     /locus_tag="V6S42_RS27235"
                     /old_locus_tag="V6S42_27235"
     CDS             complement(join(1985..2088,1..862))
                     /locus_tag="V6S42_RS27235"
                     /old_locus_tag="V6S42_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1715..1981)
                     /locus_tag="V6S42_RS27240"
                     /old_locus_tag="V6S42_27240"
     CDS             complement(1715..1981)
                     /locus_tag="V6S42_RS27240"
                     /old_locus_tag="V6S42_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(238..469)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cggcaatgac aagatcctca ttggtttctc gatccaactg gaggacgttt tttagcgccc
       61 caccggtcga gataaaacgg cgcttgtgaa gctgccgcgt cagctcaaga aaccactcag
      121 gatcgtttgc catatcttcc ggtttaacgg agtatttcag cgtttcagca accgcgcttt
      181 gcagttgctc ggcaacgttg gccacaacct cacctgtctt agtttttact gcccgaatat
      241 cgatattcgg ctcatagttc acccgcaagc aatcgcgcca gagttctacc caacgttcgt
      301 gcttaacgta gttcttccct ttaaaccaag aaggttgcac catcagcaga cagtgaaaat
      361 gcggatgtgc gctgccatcc ttacctcgcg tcacctccgt agccctgatc cacccctgaa
      421 caggtgatag ctcctttcgc ttttccattc gcttaaacgc cgcattcatt gctgtaagga
      481 ctgttccaag ttcacctatc tcgcagttcc tgacagttaa cgtcagaaac aaccatcggg
      541 aagacgggta atccacaacg attttcggta gagcctgata aaaacgggct tgccacatga
      601 gggttcttct ccactggcag acagggcaat gacggacacg gcaaaattgc gcactacgaa
      661 gccgtaggcg cgtttctccg gtttccatga tggtagacca accaaaacgt aacaaaccgc
      721 tacaggaggc catacggagt gcatagcgct cgaattcagc agcagtctgg tagatacccc
      781 cgacagaatc ggtaagcgtg cgctgggtat cccaaactgc atcacggggg ctgtagtccg
      841 aaaggaattt atcttctgac attaagcctg ctccgatctg ggcaggctgt gatttgatgt
      901 ggtatattca tcacgtacct gtgcattgcg agccagtaca tgtcaccaga gtttgccgct
      961 gaggtgacac attcgaaatg ttggggagtg ttgacgcact ccccaacgcc gattaaaaac
     1021 gcctcaattc tgctccattt tctttgacct gtcaaaaccc actcaaagcc tcgtcattac
     1081 tgggctaaac cactatttca tatccgccat ttatccctta agtataaata caaggatcac
     1141 gcgcgagaat tgcgcaaaaa ataaccacaa ttttaacccc caaaacaagc acccccgaac
     1201 ggcttacccc aaccttttcc acacacccga tcaccaaaac agggaataaa aacaaaaccc
     1261 tgtcttacag gtggatggcc tcacttcgtg aaaccatcca ccccgcataa gttttttaat
     1321 cggtggtgtc gtcaatcact aaatcggaac cccaaagggg acccccacga tttagcattt
     1381 gacgagatta actctcggag atggcatcat gcttttaacg gactttaaaa aacggtgaac
     1441 ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa cgagcaaagc gaggcggtag
     1501 aaagagagcc agcggcaggc tcaggacgat aatccgggga aaatgtacca ctcgcagagt
     1561 gttaacattt gacatcggat tgtctatcct gcattggtgt cgagttggca aggctgagga
     1621 taatgctgag gataatgcag gatagacaga gcactagaga ttgcatgcaa tctcgtacct
     1681 atcacctcag cctgccgctg gctcttttcc agaactaaga atgcgttctt aaaaatccaa
     1741 acttgatacc gtttaaatct attttcacaa aaagagtttt taccctctcg tttgttttct
     1801 gaatatataa aattaattca tcctcaaaac cttcgtgcgg atgaaaacca aatgtgccaa
     1861 aacttaatcc atcaataagc aaaataaggt ttttaaacct gtcaggaatc accgcttcac
     1921 ttcttaaaaa aaataaacca atagtcttat ttccattcag gtattcaata ttaatattca
     1981 tacgttaatc cgatttatcc ttctcagggg cgcgtttgta acgccgttta cctgaatccc
     2041 agacaaacgc cgtccgcttc ccgtcatcag tgccatcccc tacatcgt
//
