LOCUS       NZ_CP145644             2089 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-ISR-02-A plasmid p8-S1-ISR-02-A, complete
            sequence.
ACCESSION   NZ_CP145644
VERSION     NZ_CP145644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052311
            Assembly: GCF_036884415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 388.9585X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884415.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,339
            CDSs (total)                      :: 5,217
            Genes (coding)                    :: 5,010
            CDSs (with protein)               :: 5,010
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 57 of 207
            Pseudo Genes (incomplete)         :: 155 of 207
            Pseudo Genes (internal stop)      :: 34 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            Pseudo Genes (short protein)      :: 4 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145644.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-ISR-02-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p8-S1-ISR-02-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            join(1353..2089,1..229)
                     /locus_tag="V6S43_RS26685"
                     /old_locus_tag="V6S43_26690"
     CDS             join(1353..2089,1..229)
                     /locus_tag="V6S43_RS26685"
                     /old_locus_tag="V6S43_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            233..499
                     /locus_tag="V6S43_RS26690"
                     /old_locus_tag="V6S43_26695"
     CDS             233..499
                     /locus_tag="V6S43_RS26690"
                     /old_locus_tag="V6S43_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1747..1978
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cgatcctgag tggtttcttg agctgacgcg gcagcttcac aagcgccgtt ttatctcgac
       61 cggtggggcg ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgtcat
      121 tgccgacgat gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc
      181 aggtaaacgg cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat
      241 taatattgaa tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc
      301 ggtgattcct gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac
      361 atttggtttt catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac
      421 aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt
      481 tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg
      541 agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag
      601 ccttgccaac tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt aacactctgc
      661 gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc tttctaccgc
      721 ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca tccgttcacc
      781 gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc gtcaaatgct
      841 aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca ccgattaaaa
      901 aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag acagggtttt
      961 gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa gccgttcggg
     1021 ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc gcgcgtgatc
     1081 cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggtttag cccagtaatg
     1141 acgaggcttt gagtgggttt tgacaggtca aagaaaatgg agcagaattg aggcgttttt
     1201 aatcggcgtt ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca cctcagcggc
     1261 aaactctggt gacatgtact ggctcgcaat gcacaggtac gtgatgaata taccacatca
     1321 aatcacagcc tgcccagatc ggagcaggct taatgtcaga agataaattc ctttcggact
     1381 acagcccccg tgatgcagtt tgggataccc agcgcacgct taccgattct gtcgggggta
     1441 tctaccagac tgctgctgaa ttcgagcgct atgcactccg tatggcctcc tgtagcggtt
     1501 tgttacgttt tggttggtct accatcatgg aaaccggaga aacgcgccta cggcttcgta
     1561 gtgcgcaatt ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga accctcatgt
     1621 ggcaagcccg tttttatcag gctctaccga aaatcgttgt ggattacccg tcttcccgat
     1681 ggttgtttct gacgttaact gtcaggaact gcgagatagg tgaacttgga acagtcctta
     1741 cagcaatgaa tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg
     1801 ggtggatcag ggctacggag gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc
     1861 actgtctgct gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt aagcacgaac
     1921 gttgggtaga actctggcgc gattgcttgc gggtgaacta tgagccgaat atcgatattc
     1981 gggcagtaaa aactaagaca ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg
     2041 cggttgctga aacgctgaaa tactccgtta aaccggaaga tatggcaaa
//
