LOCUS       NZ_CP145641             5601 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-ISR-02-A plasmid p5-S1-ISR-02-A, complete
            sequence.
ACCESSION   NZ_CP145641
VERSION     NZ_CP145641.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052311
            Assembly: GCF_036884415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 388.9585X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884415.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:19:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,339
            CDSs (total)                      :: 5,217
            Genes (coding)                    :: 5,010
            CDSs (with protein)               :: 5,010
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 57 of 207
            Pseudo Genes (incomplete)         :: 155 of 207
            Pseudo Genes (internal stop)      :: 34 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            Pseudo Genes (short protein)      :: 4 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145641.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-ISR-02-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5-S1-ISR-02-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            complement(57..323)
                     /locus_tag="V6S43_RS26590"
                     /old_locus_tag="V6S43_26595"
     CDS             complement(57..323)
                     /locus_tag="V6S43_RS26590"
                     /old_locus_tag="V6S43_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(307..531)
                     /locus_tag="V6S43_RS26595"
                     /old_locus_tag="V6S43_26600"
     CDS             complement(307..531)
                     /locus_tag="V6S43_RS26595"
                     /old_locus_tag="V6S43_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1199..1411)
                     /locus_tag="V6S43_RS26600"
                     /old_locus_tag="V6S43_26605"
     CDS             complement(1199..1411)
                     /locus_tag="V6S43_RS26600"
                     /old_locus_tag="V6S43_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1418..1927)
                     /locus_tag="V6S43_RS26605"
                     /old_locus_tag="V6S43_26610"
     CDS             complement(1418..1927)
                     /locus_tag="V6S43_RS26605"
                     /old_locus_tag="V6S43_26610"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024131073.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGLSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1962..2666)
                     /locus_tag="V6S43_RS26610"
                     /old_locus_tag="V6S43_26615"
                     /pseudo=""
     CDS             complement(<1962..2666)
                     /locus_tag="V6S43_RS26610"
                     /old_locus_tag="V6S43_26615"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2605..2949)
                     /locus_tag="V6S43_RS26615"
                     /old_locus_tag="V6S43_26620"
     CDS             complement(2605..2949)
                     /locus_tag="V6S43_RS26615"
                     /old_locus_tag="V6S43_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2939..3115)
                     /locus_tag="V6S43_RS26620"
                     /old_locus_tag="V6S43_26625"
     CDS             complement(2939..3115)
                     /locus_tag="V6S43_RS26620"
                     /old_locus_tag="V6S43_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3755..3913
                     /locus_tag="V6S43_RS26625"
                     /old_locus_tag="V6S43_26630"
     CDS             3755..3913
                     /locus_tag="V6S43_RS26625"
                     /old_locus_tag="V6S43_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4236..4601
                     /locus_tag="V6S43_RS26630"
                     /old_locus_tag="V6S43_26635"
     CDS             4236..4601
                     /locus_tag="V6S43_RS26630"
                     /old_locus_tag="V6S43_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226331160.1"
                     /translation="MRNKKRNSEGGGVMRERTNIMLDIGLKQSLQRLSNKTKMSLSDLI
                     NQRLSGSLSVEERIGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINE
                     MMVLKEYFRAKFYDEIK"
                     /name="hypothetical protein"
     gene            4627..5172
                     /locus_tag="V6S43_RS26635"
                     /old_locus_tag="V6S43_26640"
     CDS             4627..5172
                     /locus_tag="V6S43_RS26635"
                     /old_locus_tag="V6S43_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197758.1"
                     /translation="MFFKRHLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            5238..5588
                     /locus_tag="V6S43_RS26640"
                     /old_locus_tag="V6S43_26645"
     CDS             5238..5588
                     /locus_tag="V6S43_RS26640"
                     /old_locus_tag="V6S43_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          complement(3202..3282)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.095"
                     /coverage="98.0"
                     /evalue="6.71e-19"
     Typing          3467..3864
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          complement(1084..2615)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgagtattt accataatac ctgttgtcgg catcaaaaaa gcccgcatag cgggcttcag
       61 tcgtaaactt cgcggcgatg tccaatcgtg gcggcaagga taacaagctc gtcatctcgg
      121 atctcacaca gaacgcgata atctcccacc cgatagcgcc agaactcgcc cagctcacct
      181 ttgagcggct tccctgactg gcgaggatcg gcagcaactg caatgcgtaa actcataaaa
      241 tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta acgatttgag cgcccgatcg
      301 gaatagttaa tcgtccagac caagtgactt cctcacgtct tcagacgaat gcactgcttc
      361 atcaccacgg cgaacgcgtg cagcagtttc tgctgaaaga taataatcct ccaggtcttc
      421 cagatgcgcc agtattgctt cccgcgcata aaacgtcttt gttctgccgg tttgcttcgc
      481 cagcgagtcc agacgggact caatctcatc agataaccgg atagcaagca tataaacctc
      541 ccgaacacca tttgctatac atgtatagca acagtaccac aatcaaccta aaagaacaaa
      601 aaataatctt gttagcccct cttgataatc cgtaacgcgg tcaatatatc tagcttgagg
      661 gctttcctct taactattga aaggataacg attatgggtc ttaagccggg tccgaaacga
      721 attgccgatt caacagggga acctgataaa cgccagcgtg acaataaaaa aacgccggga
      781 aatactgaca aattgaaacc cagcaaatct tcaaaaaaat aagtcagtag ttaaaagccc
      841 tccggggctt tttttgtatc tgccgttcac gctgattaaa cataacaccc gttgcccgca
      901 ccgccccgga cgggttcgcc gcgaacgggg tggcagtcag gtaaaaccgg caacaaacca
      961 cccgataaac cgccccgttc gcgacagata aattttacct ggctgcatag ccatgcagcc
     1021 aggtgaatga catccggttt cagcgtaaat gcccgatgag aaccctgttt tcctgcattt
     1081 cagcccagcg acggaccgtc atagcttcgc tgccgggaac gcgcttccgt cagttccttt
     1141 tcgtgctgcc gttgctcgtg cgcccgttct gcggccacgg ccatctcatg acgctgcact
     1201 accggctcaa acgctgcagt tgcccgctca agcgttccac ctgctgactc aagcgcgtca
     1261 cgcgctcgct cagcgccgcg ttctcccgtt gcataagccc tgacattttc cgccattccc
     1321 cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc tgctctaatg
     1381 cgctcagcaa ctgtttttcc agctcggtca tcggcactta ctccttcacc agtctcagcg
     1441 cgttctgctt cgacagcccg ttcccgaccg tccagctctg cgtcctgtct acggccgtcc
     1501 ccttcggcag caccagaaaa cgtccgttgc tgtcttccag gtacgtcacg ccccaggtct
     1561 tcgcgttcag cttcgacagc gctgcgttct gccggtcgat ttcggccaga tttgacgcta
     1621 tcaggctccc ctgataccac aacaccccgc tcaggctggc gaacagcagt cctgacacca
     1681 tcgtgatggt cagccatgtc cgcccgacca tacgcatcac gctcaggcgg ttcgactgca
     1741 tggccgcgct cataccctgc tcgtgggcgc ggatggcggc gctgattctc ttcccgcttt
     1801 cgctcagttc cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatttcgc
     1861 cggtactctg ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac
     1921 tgttcataga tggctccctt aagtcggatg tttcgccctc cgtccgggtc ggcaatgctg
     1981 atgctgctct ttgtggtgcg cacgacctca aaacctgcgc tttccagcgc ctcagtgacg
     2041 tcctgacgcg ttttaagctc ccctgacgag gcgagggcca gtaagccccg cgtaatagcc
     2101 tctgcggctt cctgcttcgt tttgggtaac gaagatggcg tcaccagcgc gcgccggttc
     2161 tccggtgcgt tcgggtcgtg cagccccagt cgcccgttca ccacggtctg ccacgcatcg
     2221 atgcgcggac ggtctgcccg gtcgtagtac ggctggagac gcctgccggt cagcagttcc
     2281 gtgttcggta ccagaaaatt cagttccagc cgccccttgt cccggtgttc aacccacagc
     2341 acgctgtact ggtctttatc gagtccgggc atcagaaccc gctcgaagct cgccatcagc
     2401 ttttcacgct ggccgggcgg taaatcctgt tcagcaaaag acaggacgcc ggaggtgtac
     2461 tttttggcgt agggcgaggc gtcgataagc tcccggacct cctccggctt tccctgcagg
     2521 acgatggccc cgtcgcgctg gcggtctttc cccagcagat aatccaccgg cccggcaccg
     2581 ccgcctcgcc ctcgcggatg aaacttaacg atcatcgtct gccccctttt ccagcacggc
     2641 atgccgcagc cgctcgagtc cggcatcgat ggccatcagc gcggccacca cctgaacccg
     2701 gtcagcgcct gaccactggc cgccgttaat tttccgggcg atctggttga ggttgttccc
     2761 catcccggca agctggcgca gcaaaaccgg gtcgatggac ggcagccgca acgaccgcgc
     2821 cgggcgcgta tcgagacagg tttcccgcat ccacactgcc agttgccttc cattgcagcg
     2881 ctccagcagc tgctggtact ccccatcggt cacccggata cctaaaaaac gggtacgctt
     2941 aaccgccatc ctctcctccg atatccgcaa aatgtgaccg ggctgaacgc tcaagctctg
     3001 ccatctgctc ccggcggtaa tcttcctcct gtgcctgacg catcagattt gccatcactg
     3061 ccggatgcca ggatcttttt tttacgacga gcggcttctt cttcggcttc tgcattatcc
     3121 ctccttcgcc aacgccggac ggcacggaaa tgttttccgg gtcgctccgg cgttggacaa
     3181 gcgccagcgc gtcagtggtg ttagcgggtt tcggggcgca gccctgaacc agtcacagcg
     3241 tttacgctgt gtatactggc ttactatgct gacatgagag cgcgattcat gaaagtgcct
     3301 catgtggcag gtgtgatatg tggctcagga gcgtccgcaa taccatgcac ggacagatga
     3361 aatgacgcct cctcgctcac tcggtcgctg cgctcctgcc gttcggctgc ggcgggcgtg
     3421 accggctcac ggataacggg ataaagcaaa ggcgctcaaa tcagaaagtg ccggagaaaa
     3481 ggatgcggcg tggccgtttt tccataggct ccgcctcccc tgacgagcat cacagaaatg
     3541 gacgctcaag tcagaggagg acgaaacccg acaggactta aagatgccgg tgatttctct
     3601 ccggcggctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgttcc
     3661 gcctttttcc ctttcgggaa gcgtggcgct ttctcatagc tcacgctgct ggtatctcgg
     3721 ctcggtttag gtcgttcgct ccaagctggg ctgtatgcac gaaccccccg ttcagcccga
     3781 ctgctgcgcc ctttccggta actatcatct tgagtccaac ccggacggac acgcaaaaac
     3841 gccactggca gcagccattg gtaactggat atatacgaga gagatattga gaggtcttga
     3901 agtgggtgcc taactgcggc tacactgaaa gaacagtttt ggtatctgcg gtctcgtata
     3961 cccagttacc cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccgcctcccc
     4021 aggcggtttt ttcgtttaca gtgcaagaga ttacgcgcag aaaaaaagga tctcaagaag
     4081 atcctttaaa tgatcgtcag ttctgttatc cccggagctc catccatccc acggaaaaac
     4141 acccgccact gatgccctca gcgcactgca catcatgtac aaaccactcc ctccctcctt
     4201 tttcattgcg tttcttaatg cgcaatggta gctttgtgcg caacaaaaaa cgcaatagcg
     4261 aggggggagg ggtaatgaga gaaagaacaa atatcatgct agatatagga ttaaagcaat
     4321 ccttacaacg gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt aaccagagat
     4381 tatcaggttc actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg atgagaaacc
     4441 ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct tcaccattaa
     4501 aagaatacca agaaaaaatg gatgagatag attcaattat taatgaaatg atggttctaa
     4561 aggaatattt cagagctaag ttttacgacg aaataaaata gacacgctat acggaggcgg
     4621 agaattatgt tttttaaacg gcatttaacg gagaaggaaa tatacaaggc agcactccca
     4681 attgttaggc ttcaagggag agttaacact tcattacgtt ttttatccaa atactcagat
     4741 gtaaacaaaa atggattaat tgcattacat cttaatttat tacattttat ctcatacaac
     4801 atacacggtg tagagtctct gaaaatcact caagcaggca taaacaaagc tgtacaacaa
     4861 agccctcacg cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa aggacctgag
     4921 tacatcgaag gattaattag aaacccatgg gggaagaaaa atgataatcc actaatattt
     4981 ataatgagca ttgaagaaag tataaaagaa acccttaact caggttatat gttatataga
     5041 gtagctccca taactgaggg tgaagcagct gctttcatgg atgttcttaa acaattatta
     5101 ttatttgagg ataattttcg cctttttggt agggacttgg tttataaaat agaaaaatta
     5161 aaaatcagat aacaaagtat ggtgaacgaa aaatgaaaga gaggatagtg aaatgtgtaa
     5221 taccaagatt ctcaactatg tattggttaa ttgcgttcct agcatttata ttccccggtc
     5281 gatcttttgc tgacggttac accgcttact accgatgtaa tggtactcag gaagcatctc
     5341 ttactgttaa agataacaaa ctaagtatgg ttgtaggtct ttctaagttt aataggcaac
     5401 tggatactta tagattcaaa gaaaagggaa tcgaagtagt aatgcagaga gctgcttctg
     5461 atgataactc tgaggccata atgtctttta ttatgcctga cgttaacaat atgaccttat
     5521 tagttaaaaa taacggctac attatcgtta agacaacctg ctggttaact aaaaccgaca
     5581 aacagtagaa cacgtagtta g
//
