LOCUS       NZ_CP145626             2125 bp    DNA     circular CON 05-APR-2024
DEFINITION  Escherichia coli strain S1-ESP-02-A plasmid p4-S1-ESP-02-A, complete
            sequence.
ACCESSION   NZ_CP145626
VERSION     NZ_CP145626.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33052315
            Assembly: GCF_036884375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Strains isolated from intestinal colonization screening for
            multidrug-resistant Enterobacterales in stool samples from healthy
            people living abroad
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Campos-Madueno,E.I., Aldeia,C. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 25, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 349.7547X
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036884375.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 17:24:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,026
            CDSs (total)                      :: 4,908
            Genes (coding)                    :: 4,657
            CDSs (with protein)               :: 4,657
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 98 of 251
            Pseudo Genes (incomplete)         :: 173 of 251
            Pseudo Genes (internal stop)      :: 45 of 251
            Pseudo Genes (multiple problems)  :: 59 of 251
            Pseudo Genes (short protein)      :: 4 of 251
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145626.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="S1-ESP-02-A"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4-S1-ESP-02-A"
                     /geo_loc_name="Switzerland"
                     /lat_lon="46.947928 N 7.422277 E"
                     /collection_date="2021"
                     /collected_by="Institute for Infectious Diseases (IFIK),
                     University of Bern"
     gene            join(1935..2125,1..775)
                     /locus_tag="V6S47_RS25120"
                     /old_locus_tag="V6S47_25120"
     CDS             join(1935..2125,1..775)
                     /locus_tag="V6S47_RS25120"
                     /old_locus_tag="V6S47_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            779..1045
                     /locus_tag="V6S47_RS25125"
                     /old_locus_tag="V6S47_25125"
     CDS             779..1045
                     /locus_tag="V6S47_RS25125"
                     /old_locus_tag="V6S47_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          204..435
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcgcctacgg cttcgtagtg cgcaattttg ccgtgtccgt cattgccctg tctgccagtg
       61 gagaagaacc ctcatgtggc aagcccgttt ttatcaggct ctaccgaaaa tcgttgtgga
      121 ttacccgtct tcccgatggt tgtttctgac gttaactgtc aggaactgcg agataggtga
      181 acttggaaca gtccttacag caatgaatgc ggcgtttaag cgaatggaaa agcgaaagga
      241 gctatcacct gttcaggggt ggatcagggc tacggaggtg acgcgaggta aggatggcag
      301 cgcacatccg cattttcact gtctgctgat ggtgcaacct tcttggttta aagggaagaa
      361 ctacgttaag cacgaacgtt gggtagaact ctggcgcgat tgcttgcggg tgaactatga
      421 gccgaatatc gatattcggg cagtaaaaac taagacaggt gaggttgtgg ccaacgttgc
      481 cgagcaactg caaagcgcgg ttgctgaaac gctgaaatac tccgttaaac cggaagatat
      541 ggcaaacgat cctgagtggt ttcttgagct gacgcggcag cttcacaagc gccgttttat
      601 ctcgaccggt ggggcgctaa aaaacgtcct ccagttggat cgagaaacca atgaggatct
      661 tgtcattgcc gacgatgtag gggatggcac tgatgacggg aagcggacgg cgtttgtctg
      721 ggattcaggt aaacggcgtt acaaacgcgc ccctgagaag gataaatcgg attaacgtat
      781 gaatattaat attgaatacc tgaatggaaa taagactatt ggtttatttt ttttaagaag
      841 tgaagcggtg attcctgaca ggtttaaaaa ccttattttg cttattgatg gattaagttt
      901 tggcacattt ggttttcatc cgcacgaagg ttttgaggat gaattaattt tatatattca
      961 gaaaacaaac gagagggtaa aaactctttt tgtgaaaata gatttaaacg gtatcaagtt
     1021 tggattttta agaacgcatt cttagttctg gaaaagagcc agcggcaggc tgaggtgata
     1081 ggtacgagat tgcatgcaat ctctagtgct ctgtctatcc tgcattatcc tcagcattat
     1141 cctcagcatt atcctcagca ttatcctcag cattatcctc agccttgcca actcgacacc
     1201 aatgcaggat agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc
     1261 ggattatcgt cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct
     1321 caacgcctca cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta
     1381 aaagcatgat gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct
     1441 ttggggttcc gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat
     1501 ggtttcacga agtgaggcca tccacctgta agacagggtt ttgtttttat tccctgtttt
     1561 ggtgatcggg tgtgtggaaa aggttggggt aagccgttcg ggggtgcttg ttttgggggg
     1621 ttaaaattgt ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag
     1681 ggataaatgg cggatatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt
     1741 tttgacaggt caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg
     1801 cgtcaacact ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta
     1861 ctggctcgca atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga
     1921 tcggagcagg cttaatgtca gaagataaat tcctttcgga ctacagcccc cgtgatgcag
     1981 tttgggatac ccagcgcacg cttaccgatt ctgtcggggg tatctaccag actgctgctg
     2041 aattcgagcg ctatgcactc cgtatggcct cctgtagcgg tttgttacgt tttggttggt
     2101 ctaccatcat ggaaaccgga gaaac
//
