LOCUS       NZ_CP145184             7823 bp    DNA     circular CON 05-APR-2024
DEFINITION  Raoultella ornithinolytica strain CAV1958 plasmid pCAV1958-7823,
            complete sequence.
ACCESSION   NZ_CP145184
VERSION     NZ_CP145184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22875475
            Assembly: GCF_036791365.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Tn5403 promotes plasmid rearrangements and degradation of the
            Klebsiella pneumoniae carbapenemase (KPC) transposon Tn4401
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2024) Biological Sciences, University of Adelaide,
            Victoria Drive, Adelaide 5005, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x and 106x
            Sequencing Technology             :: PacBio and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036791365.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:54:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,633
            Genes (coding)                    :: 5,467
            CDSs (with protein)               :: 5,467
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 54 of 166
            Pseudo Genes (incomplete)         :: 127 of 166
            Pseudo Genes (internal stop)      :: 25 of 166
            Pseudo Genes (multiple problems)  :: 36 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145184.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="CAV1958"
                     /isolation_source="Hospital waste water"
                     /db_xref="taxon:54291"
                     /plasmid="pCAV1958-7823"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2014-03-01"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(6..611)
                     /locus_tag="LMZ25_RS28690"
                     /old_locus_tag="LMZ25_28690"
     CDS             complement(6..611)
                     /locus_tag="LMZ25_RS28690"
                     /old_locus_tag="LMZ25_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            706..3603
                     /locus_tag="LMZ25_RS28695"
                     /old_locus_tag="LMZ25_28695"
     CDS             706..3603
                     /locus_tag="LMZ25_RS28695"
                     /old_locus_tag="LMZ25_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(3667..4212)
                     /locus_tag="LMZ25_RS28700"
                     /old_locus_tag="LMZ25_28700"
     CDS             complement(3667..4212)
                     /locus_tag="LMZ25_RS28700"
                     /old_locus_tag="LMZ25_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049245975.1"
                     /translation="MPLPERMKPARVTRKQMKELISQLNGILDHIDNGMDENNEELKQM
                     MADWNAKVVMPCGFSDFRDFSSWTSALDFTRMALNQEKYLDDFTWTELNDVINFIRKAE
                     GSASDHSYALQLLEINFKANPLDLIYHPDCWFNDEALFHARLTTEEIGGYLMKKSGRWL
                     NDAPDIQLVYAIPQDVYD"
                     /name="hypothetical protein"
     gene            5056..5562
                     /locus_tag="LMZ25_RS28705"
                     /old_locus_tag="LMZ25_28705"
     CDS             5056..5562
                     /locus_tag="LMZ25_RS28705"
                     /old_locus_tag="LMZ25_28705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032724462.1"
                     /translation="MMRFWAFGFLCFLCVGEASADVKVFFAPKSTTYETNTSAEIKKYL
                     EDLVGGKVVDLSDNPKAVNKSLGEYLKKIKGKTSVFFGRNEMSKYNGTVCKISITTGNT
                     APSDPILTQVFTLGTLADNNSEPCRELNNKLMSVVSRDDVFKYPAVVKDYGMLSMDMIF
                     YFNNN"
                     /name="hypothetical protein"
     gene            5587..5721
                     /locus_tag="LMZ25_RS28710"
                     /old_locus_tag="LMZ25_28710"
     CDS             5587..5721
                     /locus_tag="LMZ25_RS28710"
                     /old_locus_tag="LMZ25_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071988542.1"
                     /translation="MNGGIILFLIAMPLLVSCQANYIRDVQGGSVAPSASTTISGTSS"
                     /name="colicin release lysis protein"
     gene            complement(<5935..6045)
                     /locus_tag="LMZ25_RS28715"
                     /old_locus_tag="LMZ25_28715"
                     /pseudo=""
     CDS             complement(<5935..6045)
                     /locus_tag="LMZ25_RS28715"
                     /old_locus_tag="LMZ25_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            6459..6650
                     /locus_tag="LMZ25_RS28720"
                     /old_locus_tag="LMZ25_28720"
     CDS             6459..6650
                     /locus_tag="LMZ25_RS28720"
                     /old_locus_tag="LMZ25_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_195711769.1"
                     /translation="MTKQQTTALNMAKFIQNQSLLLLGKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6650..7072
                     /locus_tag="LMZ25_RS28725"
                     /old_locus_tag="LMZ25_28725"
     CDS             6650..7072
                     /locus_tag="LMZ25_RS28725"
                     /old_locus_tag="LMZ25_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_195711771.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMLNSGKLSYTSVVKQGKDIKVID
                     TSELIRVFGSLDGVIDSVKYDVNIDAKSTEVNNQGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHGHSSQDHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(5933..6412)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.909"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 acaccctaag cgttagctct gtgtcgttgc acgatgtcag cgacggtatt cttgctgata
       61 ccgagctcgc gtgcgatcca gcgatagctg cgtccctcgg ccctcatcgc aaccacctta
      121 ggcaaaagtc ggtctgattt tggtcgcact ccggcctgac gaccaagcct cttaccacgt
      181 gccttcgcaa cagcaaggcc tgacttgacc cgctcgctga tgagatcccg ctcaaactcc
      241 gcaatgccgg aaagaaacgt cgccagcatt cgtccatacg gcgacgaaag atcgaacgcc
      301 attccattca tggctatcac ggaaaccttc cagttctcca gttcacgtag cgtattgagc
      361 agatcgagcg tcgagcgccc ccaccgggaa agctcagtga ccaggattgc atcaatttgt
      421 ctggactggg caagcgccag gactttcttt cgctcggccc ggtcgagttt agttcctgaa
      481 cctgtttcct taaatattcc caccacgtcg tagccggcac ggccggcgaa ggctcgcaga
      541 tcaaattcct ggcgttcaca agactgatcc gctgttgaaa cccggcagta aatggcggca
      601 cgatgtccca attgaaccct cctggatttt tgtatcggaa cgccctgatt tatatgggct
      661 ggctgttgtc caaaacagac tatacttcaa aagggacgaa tttgtatgtc acgacgccat
      721 attttcaccg aacggcagcg agcagcgctg ttcgatctgc ccacggacga actgtcgcta
      781 ctgaagttct acacgctggg cgatgatgac ctggaaaaca ttaggcagcg ccgcagaccg
      841 gaaaacagga ttggctttgc cctgcaactt tgtgccttac gatatccggg ccgtgcactg
      901 gctcctggtg agatgatccc gcgtgaagtc ctttccttcg tcggtgctca gcttggagtt
      961 ccggctgatg cgcttctcac ttatgccaca cggcgccaaa cccgtcagca gcacatggac
     1021 acgctgcgcg aaatttacgg ctacaagacc ttcacgggcc gtggtgcccg tgatctgcgg
     1081 gagtggactt tcggccaggc cgaagatgcc agatcaaacg aggatcttgc tcatcgtttt
     1141 attgtgcggt gtcgggaaac ttccaccatt ctgcccgcag tatcgacaat cgagcgcttg
     1201 tgcgcggatg ctctggtcgc cgctgagcgg cggattgaaa cgcggattgc ggaaaattta
     1261 acagcggatg ttcgcgatca cctggacaaa cttctgagtg aaatgctcgc cggcaatatc
     1321 agtcgtttca tctggcttcg caacttcgag gttggtaaca actcggctgc tgctaaccgt
     1381 ttgctcgaca ggctcgaatt tctgcgtacc ctgaatatca atcatagtgc tttggccagc
     1441 atacctgccc atcgcattgc ccggctgcgt cggcagggtg aacgctactt caccgacggt
     1501 ttgcgtgaca tcacttcgga ccgccgctgg gcgatccttg ccgtctgtgt tgtggagtgg
     1561 gaagcggcga ttgctgatgc catagtcgaa acccatgaca ggatcgtagg aaaaacctgg
     1621 cgggaagcga agcgccagca tgacgaaaca atttccggct ctaaagccac actcacggat
     1681 acgatccgta ccttcaccgc gctgggagct tcgttgcttg aggcccgcag tgacggaacc
     1741 ccgctggaga tggctgtcgc cagttcggtt gcatgggacc ggctcgctca actggtagcg
     1801 acagggactc aactcagcaa cacgctagcc gatgagcctc ttgcatatgt cgggcaggga
     1861 taccatcgct ttcgtcgtta tgcgccccgc atgttgcgct gtctgaagct cgaagccgcg
     1921 ccggtcgccg gaccattggt agcagcagct ttgtcgatcg gagagatgaa aggtgttgca
     1981 tcgccagaaa ggcgtttcct gcggcccagc tccaaatgga accgtcattt acgagctcag
     2041 gaaaaaggag atacccgtct ttgggaagtg gctgtactct ttcacctccg ggatgctttt
     2101 cgttccggag atgtctggct cgctcattcg cgccgctatg gtgacctcaa gcaggtactg
     2161 gtgccgatga tcgcggcgca ggaaaatgca aaactggccg tgccttccaa cccacaggat
     2221 tggctggcag acagaaaggc gcgactcacg atcgctctta agcggctggc ccgggctgcc
     2281 cgtaacggca ctattccgca cggtagcata gaagatggaa cgttgcggat cgacaggttg
     2341 acagcagacg tgccggatgg tgccgaggca ctcatactgg atctgtatcg ccgaatgccg
     2401 tccgttcgga ttaccgacat gctgcttgaa gttgatgcag cccttggttt cacagatgcg
     2461 tttacccatc tgagaaccgg ggctccatgt cgcgaccgga tcggtctgct caacgtcctg
     2521 ctcgctgaag ggctcaatct gggcctgcgt aagatggcgg aagctacaaa cacgcatgat
     2581 tactggcagc tctcacgcct tgcccgctgg catgttgaaa gcgaagccat gaaccaggca
     2641 ttggcaattg tggtggccgc gcagggtaaa ctgccgatgt cacgcgtctg ggggatgggc
     2701 acgtcagcat cgagcgatgg tcagtttttc ccgacagcgc ggcatggcga agccatgaac
     2761 atggtcaatg ccaaatatgg ttctgttccc ggcctcaaag cgtatactca cgtaagcgac
     2821 cagttcgcgc cattcgcttg tcagtcgatc ccggcgaccg tgagcgaggc accgtatatt
     2881 ctcgatggac tactgatgaa cgaggtcggt cgccatgttc gcgaacagta tgccgataca
     2941 gcaggattca ccgaccattt gttcggagcc agtagcctgc tcggctacaa tctcgttctg
     3001 cgaatcaggg atctgccatc gaagcggttg tacgtattta atcccgatac gacccccagg
     3061 gagttacgca agttggtagg tggaaaagcc cgggaggatc ttatcgttgc gaactggcct
     3121 gatattttcc gttgtgccgc gacgatgacc gctggcaaaa tcaggcccag ccaactcctg
     3181 cgcaagctcg cttcttaccc acgacaaaac aaccttgcag ttgcgcttcg tgaagttggt
     3241 cgtattgaac ggaccctttt cattattgag tggatcctgg atacggacat gcagcggcgt
     3301 gctcagatcg gtcttaacaa gggagaggcc caccatgcgc tcaaaaatgc gctccgtatc
     3361 gggaggcagg gggaaattcg cgatcgcacg acagaggggc agcactaccg aatcgctggg
     3421 ctcaatttat tgactgcggt gatcatttac tggaataccg tccatcttgg tcatgccgtc
     3481 acggagcggc ggaacgaagg gttggatgtt ccccctgaat ttcttcccca catatcccca
     3541 ttgggctggg cgcacattct actgactggc gaatatcttt ggcccaagga accgaaagct
     3601 tagggtgtca tttcgccctc agccggaacc gaccccatta agggattatg gtaaatggtg
     3661 acttggttaa tcataaacat cctgggggat ggcatacacc agttggatat ccggtgcatc
     3721 gttgagccat cgacctgatt ttttcattaa gtagccacca atttcttcgg tagtcagccg
     3781 ggcatgaaac aatgcttcat cattgaacca gcagtcaggg tgatagatga gatccagtgg
     3841 gtttgccttg aaattaatct ccagtaactg gagtgcatag ctgtggtcag atgcactacc
     3901 ttctgctttg cgtatgaagt tgataacgtc gttcagttct gtccatgtaa agtcatcgag
     3961 gtatttttcc tgattaagcg ccatcctggt gaaatctagt gcacttgtcc aggatgaaaa
     4021 atcacgaaaa tcagagaacc cgcagggcat gacgaccttt gcattccagt ctgccatcat
     4081 ctgttttagt tcttcattgt tttcgtccat cccgttatcg atgtgatcga gaatgccatt
     4141 cagctgactg atcagttctt tcatctgttt acgggtgacc cgtgcgggtt tcattcgctc
     4201 aggtaatggc atcagaaata ctctcttttt tatcgtgcac tcgggggaac tgagtaatct
     4261 atcgggctat gaatcagagc acaaatcgtt ttttttcttt ctgatgatcc gcattctgcc
     4321 tcagatttac ctctgccgta acttttaacc gattgaaaat aaataaaaat attgttgacg
     4381 tctgcatatt gacagagctg gcatgtcaag agtagtatca aactactgta tataaacaca
     4441 tgtttttata tacactagtt tggcgtggca gagaactaat aacagcctcc ggataactgt
     4501 cctgaagcaa taaaccgcac gccggattga atcgccacgg ataatctaga cacttccgag
     4561 ccgttgataa tactggtttt catattctgt cggtgacatc tgttcgctag aaccatgccg
     4621 atgcttataa gaaaaaccca aaagaacagc aaagcattca tctttcaaat gcagatgtaa
     4681 ttgggcagtg tattaaagat gtccaaacaa cagagactgg gttacgcagt ctttaccctg
     4741 gttataatga tttctcagat ccacggaaaa ctgcattggt agatatgggg tttaaccttg
     4801 gtgttccaaa gcttaagaag gaatttgtta agtttaatga tgcagtaaat cgtggtgatt
     4861 ggaatgccgc agctgatgag agtcatcgaa caataaaaca aagaggtgac aaaaggaata
     4921 aagataccta cgttcagttg cgctataatc ggtagtggta taaatttgtt gctcccatcc
     4981 atttaagatg ggagcaagga ttttttttaa gcttattaac aataatgcat ttaatgtctt
     5041 ctatgaggta aggttatgat gcggttttgg gcttttggtt ttttatgttt tttatgtgtt
     5101 ggtgaggcat ctgctgacgt taaagttttt tttgctccaa aatctacaac atatgaaacc
     5161 aacacatctg ctgagattaa aaaatattta gaagatctag ttggtggtaa agttgtggat
     5221 ttaagtgaca accctaaagc ggtaaataaa tctctgggtg agtatctgaa aaaaataaag
     5281 ggaaagactt ctgttttttt tggtcgcaat gaaatgagca agtataatgg gaccgtttgt
     5341 aagatatcca ttacgacagg aaatactgcc ccgtcagatc ctatcttaac tcaggtgttt
     5401 acacttggaa cgttggctga taataactca gaaccttgtc gtgaattaaa taacaaacta
     5461 atgtctgtag ttagtaggga tgatgtcttt aaatatccgg cagtagttaa agattatggg
     5521 atgctatcta tggacatgat attttatttt aataataatt aatttgtttt attttggtgg
     5581 tgtatcatga acggcggaat aattttattt cttattgcaa tgccattgct agtctcatgt
     5641 caagcaaact atatcagaga tgttcaaggg ggatcggtgg ccccctcggc atctactacg
     5701 ataagtggga ctagctctta gtaacggatc ttcttgtgat cctttatcgt tcatcgtaat
     5761 ctcttgctct gtaaacgaaa aaaccacccg gcaaggtggt tttttcgaag gatcggaaac
     5821 cggcaagttt ctgaaccgag gtaactggct agttagagga gcgcagtcac caaaactgtt
     5881 cgtttagttt agccttaacc ggcgcataac ttcaagacta actcctctaa atcagttacc
     5941 aatggctgct gccagtggcg cttttgcatg tcttaccggg ttggactcaa gacgatagtt
     6001 accggataag gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     6061 gcgaactgcc ttcccggaac tgagtgtctg gcgtggaatg agataaacgc ggccataaca
     6121 gcggaatgac accggtaaac cgaaggcagg aacaggagag cgcacgaggg agccatcagg
     6181 gggaaacgcc tggtatcttt aaagtcctgt cgggtttcgc caccgctgat ttgagcgtca
     6241 tatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcaact ccgtggcttt
     6301 cttttgttcg ctctggctat ctgtgctgtc gtcttatgcc cttatacgtg agttgtcacc
     6361 gctcgccgca gccgaacggc aggagcgtag cgactgagtg agcgaggaag cggaaaagag
     6421 accggtttaa catcgagtac tgacgctctg aggcctttat gacaaaacaa cagacgaccg
     6481 cgctgaacat ggctaaattt atccagaatc agtccctgct cctgctgggg aagcttaacg
     6541 agcttgatct cgatgctgag gctgaccttt gtgagaaact ccatgatgat gccgagcatc
     6601 ttttccgtac actgtcctcg agactggatg cattacagga tggtaactga tggccagagt
     6661 gtctattagt gaagctgcac ggcttgttaa ggttagtcgc cccacaattt acaagatgct
     6721 taacagtggg aagctaagtt acacctctgt tgtaaagcaa ggtaaggata ttaaagttat
     6781 tgatacatca gagcttatta gagtttttgg ttctcttgat ggtgtaatcg acagtgtaaa
     6841 atatgatgta aatattgacg ctaagtctac agaggttaac aaccagggtt tacatgactt
     6901 acagcataga attgctttgc ttgaagctga aaacgatggc cttaaaggcg ctgttaaagc
     6961 gcgcgatgaa catatcgatt cccttcgcca ggctatgcag cttctggagc acaagcacgg
     7021 acattcttca caggatcatt ctccgtggtg gaaattctgg aaaaaatcct gatcgcggca
     7081 ttcttcagtt ttgctctccg gccattccgg ggagttcctg aaccttccca aaaatcgcca
     7141 gatttctgaa cgctcctcac cactaaaaat acccacaccc cacgcaaaat taaaattttg
     7201 aagatttgta tttaatatca gttggttacg ttaaggagat ttcgtttttt aaattttatt
     7261 tatatagaac cgactctatg acgagtcgtc atagagtccc cggcgagtcg tcatagagtc
     7321 ggggacgact caggactata aaaacaatga gttaataacg acccgactct agacccgact
     7381 ccccgtttgg ttgtttttta gtcgtggcga ctctaggtac gactctagtg aagagtcgcc
     7441 ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacg
     7501 aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcctcacgg
     7561 ccacgcaata aattacgtcc ccgttcaccg ttttcggagg agaagaacgt cagaaacagg
     7621 actttccctg gctgcacggc gtgaatttcc gcgctatgca cttgcgcgca tactcatgca
     7681 tgtcgtaaaa acagagccag cgcgtttctg gcaggttttc gggtggtttg ttgcctgttt
     7741 tgccggtttc ctgccagaaa cgccatgagg ccgttttagc ggtgcgtaca attaaggggt
     7801 cggttccggc tgagggcgaa atg
//
