LOCUS       NZ_CP145183            19705 bp    DNA     circular CON 05-APR-2024
DEFINITION  Raoultella ornithinolytica strain CAV1958 plasmid pKPC_CAV1958-20,
            complete sequence.
ACCESSION   NZ_CP145183
VERSION     NZ_CP145183.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22875475
            Assembly: GCF_036791365.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 19705)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Tn5403 promotes plasmid rearrangements and degradation of the
            Klebsiella pneumoniae carbapenemase (KPC) transposon Tn4401
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19705)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2024) Biological Sciences, University of Adelaide,
            Victoria Drive, Adelaide 5005, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x and 106x
            Sequencing Technology             :: PacBio and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036791365.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:54:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,757
            CDSs (total)                      :: 5,633
            Genes (coding)                    :: 5,467
            CDSs (with protein)               :: 5,467
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 54 of 166
            Pseudo Genes (incomplete)         :: 127 of 166
            Pseudo Genes (internal stop)      :: 25 of 166
            Pseudo Genes (multiple problems)  :: 36 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145183.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19705
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="CAV1958"
                     /isolation_source="Hospital waste water"
                     /db_xref="taxon:54291"
                     /plasmid="pKPC_CAV1958-20"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2014-03-01"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            join(18684..19705,1..4)
                     /gene="istA"
                     /locus_tag="LMZ25_RS28605"
                     /old_locus_tag="LMZ25_28605"
     CDS             join(18684..19705,1..4)
                     /gene="istA"
                     /locus_tag="LMZ25_RS28605"
                     /old_locus_tag="LMZ25_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            1..780
                     /gene="istB"
                     /locus_tag="LMZ25_RS28610"
                     /old_locus_tag="LMZ25_28610"
     CDS             1..780
                     /gene="istB"
                     /locus_tag="LMZ25_RS28610"
                     /old_locus_tag="LMZ25_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            1167..2048
                     /locus_tag="LMZ25_RS28615"
                     /old_locus_tag="LMZ25_28615"
     CDS             1167..2048
                     /locus_tag="LMZ25_RS28615"
                     /old_locus_tag="LMZ25_28615"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="WP_004152396.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     gene            complement(2298..>3263)
                     /locus_tag="LMZ25_RS28620"
                     /old_locus_tag="LMZ25_28620"
                     /pseudo=""
     CDS             complement(2298..>3263)
                     /locus_tag="LMZ25_RS28620"
                     /old_locus_tag="LMZ25_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(3297..6194)
                     /locus_tag="LMZ25_RS28625"
                     /old_locus_tag="LMZ25_28625"
     CDS             complement(3297..6194)
                     /locus_tag="LMZ25_RS28625"
                     /old_locus_tag="LMZ25_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            6289..6894
                     /locus_tag="LMZ25_RS28630"
                     /old_locus_tag="LMZ25_28630"
     CDS             6289..6894
                     /locus_tag="LMZ25_RS28630"
                     /old_locus_tag="LMZ25_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            7003..>7431
                     /locus_tag="LMZ25_RS28635"
                     /old_locus_tag="LMZ25_28635"
                     /pseudo=""
     CDS             7003..>7431
                     /locus_tag="LMZ25_RS28635"
                     /old_locus_tag="LMZ25_28635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7472..7969
                     /locus_tag="LMZ25_RS28640"
                     /old_locus_tag="LMZ25_28640"
     CDS             7472..7969
                     /locus_tag="LMZ25_RS28640"
                     /old_locus_tag="LMZ25_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            7976..8191
                     /locus_tag="LMZ25_RS28645"
                     /old_locus_tag="LMZ25_28645"
     CDS             7976..8191
                     /locus_tag="LMZ25_RS28645"
                     /old_locus_tag="LMZ25_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            8627..9460
                     /locus_tag="LMZ25_RS28650"
                     /old_locus_tag="LMZ25_28650"
     CDS             8627..9460
                     /locus_tag="LMZ25_RS28650"
                     /old_locus_tag="LMZ25_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196747.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcoRI family type II restriction endonuclease"
                     /protein_id="WP_102016632.1"
                     /translation="MSNKNQSNRLTDQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKDA
                     LSKEYPQLSFRYRDSIRKTEINESLKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDHDEW
                     RVVLVTEAKHQGKDIINIKNGVLVGKSSDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYILFLEGSNFLTENILITRPDGRVVNLEYKSGMLNRLDRLTAANYGMPINSNLCVNKF
                     VKHKDKNIMLQAASIYTQGNGEKWDSKIMFEIMFDISTTSLRVLGRDLFDQLTSK"
                     /name="EcoRI family type II restriction endonuclease"
     gene            9498..10475
                     /locus_tag="LMZ25_RS28655"
                     /old_locus_tag="LMZ25_28655"
     CDS             9498..10475
                     /locus_tag="LMZ25_RS28655"
                     /old_locus_tag="LMZ25_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="WP_102016631.1"
                     /translation="MARNATNKLLQKAKKLKSDEFYTQYCDIENELQYYKSQFSGKSVY
                     CNCDDPRVSNFFKYFSVNFHELGLEKLIASCYVERDVDIFSNGVVKNGFYYEYRGESKE
                     GCVLDDIKPSYFDGDGDFRSSESIELLKKSDVVVTNPPFSLFREYLEQLIKYEKKFLII
                     ANVNSITYKEVFKLIKDNKLWLGVHLGRGVSGFIVPEHYELYGTEARIDASGNRIISPN
                     NCLWLTNLDTFIRHEDIPLTKKYFGNEAEYPKYDNFDAINVNKTKDIPSDYKGVMGVPI
                     TFLHKFNPDQFELIKFRKGMDGKDLSINGKCPYFRILIKNKRLQ"
                     /name="adenine-specific methyltransferase EcoRI family
                     protein"
     gene            complement(10761..10910)
                     /locus_tag="LMZ25_RS28660"
                     /old_locus_tag="LMZ25_28660"
     CDS             complement(10761..10910)
                     /locus_tag="LMZ25_RS28660"
                     /old_locus_tag="LMZ25_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_102016630.1"
                     /translation="MHEPPVQSDCCASSGNYRLESNPERHDKAPLAAAIGRQNVREMYR
                     EFLK"
                     /name="replication initiation protein"
     gene            11540..11881
                     /locus_tag="LMZ25_RS28665"
                     /old_locus_tag="LMZ25_28665"
     CDS             11540..11881
                     /locus_tag="LMZ25_RS28665"
                     /old_locus_tag="LMZ25_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            11871..12056
                     /locus_tag="LMZ25_RS28670"
                     /old_locus_tag="LMZ25_28670"
     CDS             11871..12056
                     /locus_tag="LMZ25_RS28670"
                     /old_locus_tag="LMZ25_28670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338482669.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVGSVPAEGEMTP"
                     /name="hypothetical protein"
     gene            complement(12053..>13660)
                     /locus_tag="LMZ25_RS28675"
                     /old_locus_tag="LMZ25_28675"
                     /pseudo=""
     CDS             complement(12053..>13660)
                     /locus_tag="LMZ25_RS28675"
                     /old_locus_tag="LMZ25_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330913.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(13723..15438)
                     /gene="tnpR"
                     /locus_tag="LMZ25_RS28680"
                     /old_locus_tag="LMZ25_28680"
     CDS             complement(13723..15438)
                     /gene="tnpR"
                     /locus_tag="LMZ25_RS28680"
                     /old_locus_tag="LMZ25_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            15548..18577
                     /gene="tnpA"
                     /locus_tag="LMZ25_RS28685"
                     /old_locus_tag="LMZ25_28685"
     CDS             15548..18577
                     /gene="tnpA"
                     /locus_tag="LMZ25_RS28685"
                     /old_locus_tag="LMZ25_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     ARG             1168..2045
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          11273..11446
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(10752..11349)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.917"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6926..8307
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.566"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11562..11875
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.36e-58"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tggacgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gctcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagt
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attggggtcg gttccggctg agggcgaaat gacaccctaa
     3301 gctttcggtt ccttgggcca aagatattcg ccagtcagta gaatgtgcgc ccagcccaat
     3361 ggggatatgt ggggaagaaa ttcaggggga acatccaacc cttcgttccg ccgctccgtg
     3421 acggcatgac caagatggac ggtattccag taaatgatca ccgcagtcaa taaattgagc
     3481 ccagcgattc ggtagtgctg cccctctgtc gtgcgatcgc gaatttcccc ctgcctcccg
     3541 atacggagcg catttttgag cgcatggtgg gcctctccct tgttaagacc gatctgagca
     3601 cgccgctgca tgtccgtatc caggatccac tcaataatga aaagggtccg ttcaatacga
     3661 ccaacttcac gaagcgcaac tgcaaggttg ttttgtcgtg ggtaagaagc gagcttgcgc
     3721 aggagttggc tgggcctgat tttgccagcg gtcatcgtcg cggcacaacg gaaaatatca
     3781 ggccagttcg caacgataag atcctcccgg gcttttccac ctaccaactt gcgtaactcc
     3841 ctgggggtcg tatcgggatt aaatacgtac aaccgcttcg atggcagatc cctgattcgc
     3901 agaacgagat tgtagccgag caggctactg gctccgaaca aatggtcggt gaatcctgct
     3961 gtatcggcat actgttcgcg aacatggcga ccgacctcgt tcatcagtag tccatcgaga
     4021 atatacggtg cctcgctcac ggtcgccggg atcgactgac aagcgaatgg cgcgaactgg
     4081 tcgcttacgt gagtatacgc tttgaggccg ggaacagaac catatttggc attgaccatg
     4141 ttcatggctt cgccatgccg cgctgtcggg aaaaactgac catcgctcga tgctgacgtg
     4201 cccatccccc agacgcgtga catcggcagt ttaccctgcg cggccaccac aattgccaat
     4261 gcctggttca tggcttcgct ttcaacatgc cagcgggcaa ggcgtgagag ctgccagtaa
     4321 tcatgcgtgt ttgtagcttc cgccatctta cgcaggccca gattgagccc ttcagcgagc
     4381 aggacgttga gcagaccgat ccggtcgcga catggagccc cggttctcag atgggtaaac
     4441 gcatctgtga aaccaagggc tgcatcaact tcaagcagca tgtcggtaat ccgaacggac
     4501 ggcattcggc gatacagatc cagtatgagt gcctcggcac catccggcac gtctgctgtc
     4561 aacctgtcga tccgcaacgt tccatcttct atgctaccgt gcggaatagt gccgttacgg
     4621 gcagcccggg ccagccgctt aagagcgatc gtgagtcgcg cctttctgtc tgccagccaa
     4681 tcctgtgggt tggaaggcac ggccagtttt gcattttcct gcgccgcgat catcggcacc
     4741 agtacctgct tgaggtcacc atagcggcgc gaatgagcga gccagacatc tccggaacga
     4801 aaagcatccc ggaggtgaaa gagtacagcc acttcccaaa gacgggtatc tcctttttcc
     4861 tgagctcgta aatgacggtt ccatttggag ctgggccgca ggaaacgcct ttctggcgat
     4921 gcaacacctt tcatctctcc gatcgacaaa gctgctgcta ccaatggtcc ggcgaccggc
     4981 gcggcttcga gcttcagaca gcgcaacatg cggggcgcat aacgacgaaa gcgatggtat
     5041 ccctgcccga catatgcaag aggctcatcg gctagcgtgt tgctgagttg agtccctgtc
     5101 gctaccagtt gagcgagccg gtcccatgca accgaactgg cgacagccat ctccagcggg
     5161 gttccgtcac tgcgggcctc aagcaacgaa gctcccagcg cggtgaaggt acggatcgta
     5221 tccgtgagtg tggctttaga gccggaaatt gtttcgtcat gctggcgctt cgcttcccgc
     5281 caggtttttc ctacgatcct gtcatgggtt tcgactatgg catcagcaat cgccgcttcc
     5341 cactccacaa cacagacggc aaggatcgcc cagcggcggt ccgaagtgat gtcacgcaaa
     5401 ccgtcggtga agtagcgttc accctgccga cgcagccggg caatgcgatg ggcaggtatg
     5461 ctggccaaag cactatgatt gatattcagg gtacgcagaa attcgagcct gtcgagcaaa
     5521 cggttagcag cagccgagtt gttaccaacc tcgaagttgc gaagccagat gaaacgactg
     5581 atattgccgg cgagcatttc actcagaagt ttgtccaggt gatcgcgaac atccgctgtt
     5641 aaattttccg caatccgcgt ttcaatccgc cgctcagcgg cgaccagagc atccgcgcac
     5701 aagcgctcga ttgtcgatac tgcgggcaga atggtggaag tttcccgaca ccgcacaata
     5761 aaacgatgag caagatcctc gtttgatctg gcatcttcgg cctggccgaa agtccactcc
     5821 cgcagatcac gggcaccacg gcccgtgaag gtcttgtagc cgtaaatttc gcgcagcgtg
     5881 tccatgtgct gctgacgggt ttggcgccgt gtggcataag tgagaagcgc atcagccgga
     5941 actccaagct gagcaccgac gaaggaaagg acttcacgcg ggatcatctc accaggagcc
     6001 agtgcacggc ccggatatcg taaggcacaa agttgcaggg caaagccaat cctgttttcc
     6061 ggtctgcggc gctgcctaat gttttccagg tcatcatcgc ccagcgtgta gaacttcagt
     6121 agcgacagtt cgtccgtggg cagatcgaac agcgctgctc gctgccgttc ggtgaaaata
     6181 tggcgtcgtg acatacaaat tcgtcccttt tgaagtatag tctgttttgg acaacagcca
     6241 gcccatataa atcagggcgt tccgatacaa aaatccagga gggttcaatt gggacatcgt
     6301 gccgccattt actgccgggt ttcaacagcg gatcagtctt gtgaacgcca ggaatttgat
     6361 ctgcgagcct tcgccggccg tgccggctac gacgtggtgg gaatatttaa ggaaacaggt
     6421 tcaggaacta aactcgaccg ggccgagcga aagaaagtcc tggcgcttgc ccagtccaga
     6481 caaattgatg caatcctggt cactgagctt tcccggtggg ggcgctcgac gctcgatctg
     6541 ctcaatacgc tacgtgaact ggagaactgg aaggtttccg tgatagccat gaatggaatg
     6601 gcgttcgatc tttcgtcgcc gtatggacga atgctggcga cgtttctttc cggcattgcg
     6661 gagtttgagc gggatctcat cagcgagcgg gtcaagtcag gccttgctgt tgcgaaggca
     6721 cgtggtaaga ggcttggtcg tcaggccgga gtgcgaccaa aatcagaccg acttttgcct
     6781 aaggtggttg cgatgagggc cgagggacgc agctatcgct ggatcgcacg cgagctcggt
     6841 atcagcaaga ataccgtcgc tgacatcgtg caacgacaca gagctaacgc ttagggtgtc
     6901 atttcgccct cagccggaac cgacccctat gtgaaaaaat atacctcggg ggtgctgtca
     6961 ttcgcggagg cggacctgcc gccgggtcag cgtgaaaaac tgatggccag ctttgagcgg
     7021 gttctgatgc ccggactcga taaagatcag tacagcattc tgtgggtgga acacgcagac
     7081 aagggtcgtc ttgagctgaa ttttctgatc cccaataccg aattactgac ggggaaacgc
     7141 ctccagccgt attacgatcg tgccgaccgg ccgcgcatcg atgcgtggca gacagtggtc
     7201 aacggcaggc tggggctaca tgatccgaac gacccggaga accggcgggc gctggtcacg
     7261 ccgtcaggac tgccagaaac gaagcaggaa gccgcagaga cgattacacg gggtttgctg
     7321 atgttagcct catcaggaga gctgaaaacg cgtgatgacg tcacaggggc gctcacagcg
     7381 gccggttttg aggtggtcag aaccacaaaa aacagtatca gcattgccga cccggacggg
     7441 gggcgaaata tccgactgag gggagccatt tatgagcagt cttttaacgg cctcgaggga
     7501 cttcgaggag aaatcgaaag cacagcagca gcttaccgaa gagaggctga aagccgcatt
     7561 cagcaagcac gagaacgttg tcaaatcgga actgaacgca agcgcgaaga gaatcaacga
     7621 cgccatcagc gcccacgaac aggggatgaa cgcggcgatg cagtcgaacc ggctgagcgt
     7681 gctgcgaatg gtcgggcgga cgtggctgac catcaccatg gtgtcagggc tgctgttcgc
     7741 cagcctgagc ggggtgctgt ggtaccaggg gaaccggata gcggcgaacc tggcggaaat
     7801 caggcagcag agagatacgc tgtcgaaact gcagatgcag acgtggggcg tgacctatct
     7861 ggagaaccgc aacggacggt ttctggtgct gccggagggg atgaaagccg aaacgggccg
     7921 gacggtggac aacaagacgc ggaacgcggt gaagctggta cgggagtaga gcagaatgac
     7981 ggaactggaa aaacaattgc tgagcgcgtt agagcagcta cagcaggact actcgagaag
     8041 gctggacgag tgggagagcg ccttcgagga atggcggaag atgtctggtc ttatgcaacg
     8101 ggagaacgcg gcgctgagcg agcgcgtgtc gggcttgagc aggcaggtgc agagtttgag
     8161 cgagcagctg cgccggttat cgacaggctg aatgagattg agcaacagcg atacgggcaa
     8221 agaacggaaa aggaaccaga gaagacagca gatctggtac cggaacggca acgcacatta
     8281 tctcatggtg acagggagta tggagactga taaacgcccg tctcaggtgg cctgagcgtg
     8341 acggggatca tggccatttt ctgcgatttc agcacacaga gagtcttttt taaccgaaat
     8401 caccggctat gcagtgatgt gaataagtga tgcatatgcg taaaataaat caggtgcgtt
     8461 tctggtggtt ttcggtgtac ttgccaccac cttttgcccg tcagccatca gaaacgccct
     8521 gagtgcctcc ggcgcggtgc gggtaacaga tgttatggta aattaattat atggtaacct
     8581 atcgataata cgtaaatata taaaataaca gtggaaagat tgatacatgt ctaacaagaa
     8641 ccagtcaaat agattaactg atcaacataa gttatctcaa ggtgtaattg ggatttttgg
     8701 ggattatgca aaagctcatg atctcgcggt tggcgaagtt tcaaaattag taaaggatgc
     8761 tcttagcaaa gaatatcctc aattatcttt tcgatataga gatagtataa ggaaaacgga
     8821 aataaatgaa tccttaaaaa aaatcgaccc tgatctagga gggactcttt ttgtttcaaa
     8881 ttccagcatc aagcctgatg gtggaattgt agaggttaaa gacgaccatg atgaatggag
     8941 agttgtgctt gttacggaag ccaaacatca aggtaaagat ataataaata taaaaaatgg
     9001 tgtattggtt gggaaatcca gcgaccaaga tttaatggct gctggtaatg ctatcgaaag
     9061 gtctcataaa aacatatcag aaatagcaaa ttttatgctt tctgagagtc actttccgta
     9121 tatacttttt ttagaaggtt ctaacttttt aacggaaaat attttaataa caagaccgga
     9181 tggaagggtt gttaatcttg aatataagtc tggcatgtta aataggttag accgactgac
     9241 tgcagctaat tacgggatgc ctataaatag taatctttgt gttaacaagt ttgtaaaaca
     9301 taaagataaa aacattatgc tacaagcagc gtctatatat actcaaggga atggagaaaa
     9361 atgggattca aaaattatgt ttgaaataat gtttgatatt tctacaactt cgctcagagt
     9421 gttgggtcga gacttgtttg atcagcttac atctaagtga tgtgtaataa agtattgata
     9481 tgtaaggtat tttttaaatg gctagaaatg caacaaacaa attactgcaa aaagctaaaa
     9541 agttgaaaag cgatgaattt tacactcagt attgtgatat tgaaaatgaa cttcaatact
     9601 acaaatccca attttctggg aaatctgttt attgcaattg tgacgatcct agagtgagta
     9661 atttttttaa atatttctca gtaaattttc atgagcttgg cttggaaaag ttaatagcat
     9721 cttgctatgt tgaaagagat gtggatattt ttagcaacgg agtggttaag aacggttttt
     9781 attatgaata ccgtggggaa agtaaggagg ggtgtgtttt agatgatatt aaaccatctt
     9841 attttgatgg agatggtgat tttcgcagtt ctgaaagtat agaactgcta aaaaaatcag
     9901 atgttgttgt tacgaatccg ccgttctcgt tgtttagaga gtatcttgaa caattaatta
     9961 agtatgagaa gaagtttctt ataattgcca atgttaattc aataacatat aaagaagtgt
    10021 ttaaactcat taaggacaat aaactttggc ttggagttca tctcgggagg ggggtttccg
    10081 gatttatagt tcctgaacat tatgaattat atgggaccga ggcaaggatt gatgcgagtg
    10141 gaaatagaat tatttcacca aataactgtc tatggctgac aaacttggat acttttatcc
    10201 gtcatgaaga tatacctctt accaaaaaat attttggtaa tgaagctgag tatcctaaat
    10261 atgataattt tgatgctata aatgtaaata aaacaaaaga catcccatca gattacaagg
    10321 gggttatggg agtgcctata acattcctac ataaatttaa tcctgatcag tttgaattga
    10381 taaaatttag aaagggaatg gacggcaaag atttatctat taatgggaag tgcccatact
    10441 tcaggattct aataaaaaac aaaaggttac agtagtgatt aatgtgggct tatgcggtag
    10501 agctttatag taaaagccga attatttatt aaaagctgcc tttggtctca ttgtaacaac
    10561 gtgatgtgta gggttttgtt aggtttttct tgagatcatt agctggtcgt cgtaatcttc
    10621 agcaccagaa acgaaaaaac caccctggca ggtggttttt cgaaggttag ataatcctgg
    10681 cagattctct aaccgtggta acagtcttgt gcgagacatg taaccaaatt cgtccttcca
    10741 gtgtagcctc agttaggcca ctacttcaag aactctcgat acatctctcg cacattctgt
    10801 ctaccaatgg ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga ctcaagtcga
    10861 tagttaccgg aagaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc
    10921 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
    10981 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagct
    11041 accaggggga aacgcctggg atctttatag tcctgtcggg tttcgccacc actgatttga
    11101 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttggcgc
    11161 ggccttatcg tttccctgtt aagttccttc ctagcatcta ccaggaaatt tccgccccgt
    11221 tcgtaagccc gcaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
    11281 gaagcggaat atatcctgta tcgcatattc cgctgacgcg ccggtgccac ctttttcctc
    11341 cttccacatg aagcacttca ctgatatccg catccgtgcc aacatagtaa gccagtatac
    11401 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct aaaagccact
    11461 gacgcgcctg cggcttgtcc aaccccgcgc cgaccgggaa aggctttccc gtccgtcacg
    11521 gcgttatcag gaggcgattc tggcagataa acgcaacaag atgctcacga tgtgggtgac
    11581 ggaagacgaa caccggcggt tgcttgagcg gtgcgacgga aaacaactgg cggcgtggat
    11641 gcgccagacc tgccttgatg agaaaccgac acgcgccggc aggcttccct cgatttcgcc
    11701 cgcgctgctg cgtcagcttg ctggcatggg caataacctg aaccagatcg cccggaaagt
    11761 gaacgccggt ggtgccggtc atgaccgggt gcagatcgtg gcagcgctga tggcgatcga
    11821 tgccggtctg gaacggttgc ggcatgccgt actggaaaaa gggccggacg atgattgtta
    11881 aattccatcc ccggggtcgc ggcgggggcg ccggtcctgt ggattatctt ctgggtaaag
    11941 accggcagcg tgaaggtgcc agcgtcctgc aggggaagcc ggaagaagtc cgggagctga
    12001 ttgatgcgtc gccgtatgtg gggtcggttc cggctgaggg cgaaatgaca ccctaagctt
    12061 tcggttcctt gggccaaaga tattcgccag tcagtagaat gtgcgcccag cccaatgggg
    12121 atatgtgggg aagaaattca gggggaacat ccaacccttc gttccgccgc tccgtgacgg
    12181 catgaccaag atggacggta ttccagtaaa tgatcaccgc agtcaataaa ttgagcccag
    12241 cgattcggta gtgctgcccc tctgtcgtgc gatcgcgaat ttccccctgc ctcccgatac
    12301 ggagcgcatt tttgagcgca tggtgggcct ctcccttgtt aagaccgatc tgagcacgcc
    12361 gctgcatgtc cgtatccagg atccactcaa taatgaaaag ggtccgttca atacgaccaa
    12421 cttcacgaag cgcaactgca aggttgtttt gtcgtgggta agaagcgagc ttgcgcagga
    12481 gttggctggg cctgattttg ccagcggtca tcgtcgcggc acaacggaaa atatcaggcc
    12541 agttcgcaac gataagatcc tcccgggctt ttccacctac caacttgcgt aactccctgg
    12601 gggtcgtatc gggattaaat acgtacaacc gcttcgatgg cagatccctg attcgcagaa
    12661 cgagattgta gccgagcagg ctactggctc cgaacaaatg gtcggtgaat cctgctgtat
    12721 cggcatactg ttcgcgaaca tggcgaccga cctcgttcat cagtagtcca tcgagaatat
    12781 acggtgcctc gctcacggtc gccgggatcg actgacaagc gaatggcgcg aactggtcgc
    12841 ttacgtgagt atacgctttg aggccgggaa cagaaccata tttggcattg accatgttca
    12901 tggcttcgcc atgccgcgct gtcgggaaaa actgaccatc gctcgatgct gacgtgccca
    12961 tcccccagac gcgtgacatc ggcagtttac cctgcgcggc caccacaatt gccaatgcct
    13021 ggttcatggc ttcgctttca acatgccagc gggcaaggcg tgagagctgc cagtaatcat
    13081 gcgtgtttgt agcttccgcc atcttacgca ggcccagatt gagcccttca gcgagcagga
    13141 cgttgagcag accgatccgg tcgcgacatg gagccccggt tctcagatgg gtaaacgcat
    13201 ctgtgaaacc aagggctgca tcaacttcaa gcagcatgtc ggtaatccga acggacggca
    13261 ttcggcgata cagatccagt atgagtgcct cggcaccatc cggcacgtct gctgtcaacc
    13321 tgtcgatccg caacgttcca tcttctatgc taccgtgcgg aatagtgccg ttacgggcag
    13381 cccgggccag ccgcttaaga gcgatcgtga gtcgcgcctt tctgtctgcc agccaatcct
    13441 gtgggttgga aggcacggcc agttttgcat tttcctgcgc cgcgatcatc ggcaccagta
    13501 cctgcttgag gtcaccatag cggcgcgaat gagcgagcca gacatctccg gaacgaaaag
    13561 catcccggag gtgaaagagt acagccactt cccaaagacg ggtatctcct ttttcctgag
    13621 ctcgtaaatg acggttccat ttggagctgg gccgcaggaa acgcctttct ggggttctaa
    13681 gcgggaatcc cagaaaattc cgtcattccg tatgatgaaa attcagtcga ctcctcgctt
    13741 gacgtgacga gcgccaaaag cctgccgcat ctgcctggcg cgctcgagct catcgccatg
    13801 caaatacatc gacgtcgtgg cgatcgacgc atggcgtagg ttgtcgcgca ccgcggtgag
    13861 ctgagctccc cgggccaacg catggctggc atgactgtga cgcatccaat gaggcgtggc
    13921 acggcgcagc ttttcggcgg cgaccggatg atcggcctca atggcctcag cggcttgcgt
    13981 aaagaatctt cgcaacacgc accaaaggtg agggccggtg atgccggcat taccgtcttc
    14041 cccaagactg cccaccaggg gcgttttcgg attccaccgt tcccggctga ctggcagttg
    14101 acgctgtgcc aagtattggt ccagcgccgt atacgcgagc ggcggcaagg ccaccttacc
    14161 tggtttgcca cctttgccaa ccaaatgcag ccagtggtca ccgtgctcgt cgaggcggac
    14221 attacccagg acggcgccga ccagttcgct agcgcgcagg cccgtggcat agccgaagtc
    14281 gagcatgaag cgcaggcgtt gtgctgccgg ctcggaccag ccgtaggacc attccaggcc
    14341 gtctgcaatg gcgcgcagca gtagccattc tccctcggta aagcctcgcg ccgtatccag
    14401 tgcgggccgc aaggcgtgac cgcgtacctt gacgccggcg aaagggttgg ccagcacata
    14461 gcgctgctcg atgagccacc ggaacagcgc cgagagcacc gtcagcgcat acgctgccga
    14521 gcgtgcggac aagccgcccg agaaggggcg ccattccacc gaatcgcgtg gtcgaggtgg
    14581 ccccacccaa cgctcgcgcg gggtcgggtg ccggacaaag gcccggtacg cgattgcatc
    14641 gtccgtggtc agggatgata gcgcccgtcc gcgttcgacg atggcccaca ggattagccg
    14701 cgcagcctcc ttgcgatagg cgcgctgtgt ggcgacagtc tcgtgcagcg ccagccaggc
    14761 ctgcacggcc tcgtagtcgt tggtcgcatt cagcgtgcaa gcgttgcgcg gggcccggaa
    14821 attgccctgc gagccgtcga cttcgttcgg cagacgcaac tgctcccacg gcacgatgcc
    14881 gctgggctga ataatcggga tcagggcccg ggcgcgctcg gtgagctgcg gatgtgcggc
    14941 aaagaaagcc tcgacctgcc gggcgcctgt catgccgagt ccctcgatgg ctttccacca
    15001 gccacgccgg cggggaatgc gcacggtcag cgccgagagg gtgtggatac cgtacgcctg
    15061 cagtgccctg acggtcctcg gagacagcca ctggtcgatg ctgtcgctga tctggggctt
    15121 cggcgccggc agcgtgggca agagccggat cgcgcgttcg acggcacccg cgcgcccctg
    15181 tcgctcttcg ggggcgtgct ggaacacgtc ggccagatcc ggccggtggc ggctgttggc
    15241 gaaaacgatg agctggcggc ggatatgccc cagcatccct cgcgctgatt gaccggcggc
    15301 cttgcgtgcc gccaggtagt gttcaacggc cgcgcgagag ctgagtcccg cgtaccagcc
    15361 gcgcaaggca gccagcgaat ccgcatcggg aaagccgact ggggtagaac tcgaagccgt
    15421 agtctggccc gttttcatat ggacaggttg cgattcgaaa agcgagtaac gcgataataa
    15481 cgattatttc agtagaatcg aaaatgcccc atgtttctac gaaacctcaa gatagcaaga
    15541 acgggccatg agcaagaaga acaagctact cagcgtccta tccgacgccg aacaggaggc
    15601 cttgtatggc ctgccggagt tcgatgatgc gcagcagctg gaatatctgg ctgtgacaga
    15661 aaccgagttg gcgcttgcca acagccggcc cagtcttcat gccaaggtct attgcctctt
    15721 gcagatcggc tacttcaagg ccaagcatgc cttcttcagt ttcgactggg acgaagtcga
    15781 ggacgattgc gctttcgtgc tgagccgcta tttccacggc gaggtgttcg aacccaaggc
    15841 gatcaccaag catgaacgct acacccagcg cgagcagatc gccaaactat tcggctactg
    15901 gccgtggtcg gccgccttcc tgccgcagct cgagcagcag gccgcacgga ccgtgcggcg
    15961 tgacgtaacg ccggggttcg tcgccgccga gctgatcgtc tggctcaatg agcacaagat
    16021 tatccggccc ggctatacga ccttgcaaga gttggtcagc gaaacgctat ccgccgagcg
    16081 ccggcgcctg ggcaatctgc tggagcaagc gttggacgaa tctgccaagg ccgcgctgag
    16141 ccagcttctg gtccgtgatg acaccctgtc gcaactggcg gcgctcaagc aggacgccaa
    16201 gaatttcggc tggcgccaga tggtccgcga gcgcgaaaag cgcgccacgc tggagccgct
    16261 gcacgcaatc gccaagacac tgctgcctaa actcgacgtc tcgcagcaga acctgctgta
    16321 ctacgcgagc ctggcaaact tctacaccgt ccacgacctg cgcaatctga aggccgatca
    16381 gacccaactc tacctgctgt gctatgcctg ggtacgctac cggcagctct ccgacaacct
    16441 ggtcgatgcg atggcgtacc acatgaagca gttggaggac gaaagcagcg ctggtgccaa
    16501 acaatccttt gccgacgagc aggtgcgccg tcagcaggaa acgccgcggg taggccgcct
    16561 gctgtcactc tacgttgatg atagcgtggc cgatcccacg cccttcggcg aggtgcggca
    16621 gcgcgcctac aagatcatgc ctcgcgatac gctgcaaacc acggcgcagc gtatgagcgt
    16681 gaagccggcg agcaagctgg ccttgcagtg gcaggcggtg gacgggctgg ccgaccgcat
    16741 gcgccgccat cttcgtccac tgtacgttgc gctcgacctc tccggctcca atccagacag
    16801 tccatggctt gccgcgctgg cctgggccaa aggtgttttt gccaagcagc agcggctgtc
    16861 gcaacggcca ctcgacgagt ggcccgcggc gacacttccg aaacgcttgc ggccgtacct
    16921 gctgaccttc gacgccgacg gtaaaccaac cggcctgcac gctgaccgct acgagttctg
    16981 gctgtaccgc cagatcagga agcgtctcca atcgggtgaa ctctacctcg acgacagcct
    17041 gcaacaccgg cacttctcag atgaactggt tgagatggac aggaagatcg atgtgctcgc
    17101 aaaaatggag atccccttcc tgcagcaacc cgtccatgcc caactcgatg cgctgactgc
    17161 cgaactgcgc acgcagtggc tggcgttcaa ccgcgagctg aaacagggca agctgacgca
    17221 cctcgaatac aacaaggaca cgcagaaact gacctggcgc aagcccaaga gcgagaatga
    17281 gaaagcgcgc gaaaaggcat tctacgagaa gcttccgttc tgcgacgtgg ccgacgtgtt
    17341 tcgcttcgtc aacgacgagt gcaagtttct gtcggcgttc acgcccatgc agccgcgcta
    17401 cgcgaagaag gtcgccgacg ctgacagcct gatggcggtc atcatcgcgc aagcgatgaa
    17461 ccacggcaac cacgtcatgg cgcgcaccag cgacatcccg taccacgtgc tggatagcgg
    17521 ctaccagcag tacctgcgcc aggcaacgct gcacgcggcc aacgactgca tcagcaacgc
    17581 catcgccacg ctaccgatct tcccatacta ctcgttcgac ctcgaaacgc tgtacggtgc
    17641 cgtcgacggg cagaaattca gcgtcgaacg gccgaccgtg aaggcgcgct attcgcgcaa
    17701 gtacttcggg cgcggcaagg gcgtggtcgc ctacacgctg ctgtgcaacc acatcccgct
    17761 caacggctac ctgatcggcg cgcacgacta cgaggcccac cacgtgttcg acatctggta
    17821 tcgcaacacg tcggacatca tgccgaccgc gatcaccggc gacatgcaca gcgtcaacaa
    17881 ggccaacttc gccatcctgc attggtttgg ccggcggttc gagccgcgct tcaccgacct
    17941 caacgcgcaa ttgaaagaac tctactgcgc tgacgatcca gcacagtatc aggcgtgcct
    18001 gattcggcca gtcgggaaaa tcgactgcga tctcatccat cgcgagaagc cgaacatcga
    18061 ccggatcgtc gccacactcg ggctgaagga aatgacgcag ggcacgctga tccgcaagct
    18121 gtgcacctac accacgacga acccgacgcg gcgcgcgatc ttcgagttcg acaagctcat
    18181 ccgcagcatc tacacgcttc gctacctgcg cgatccgcaa ctggaacgca acgtgcaccg
    18241 ctcgcaaaat cgcctcgagt cctaccatca gctacgctcg gccattgccc aggtcggcgg
    18301 taagaaggaa ctgaccggtc gcaccgacat tgaaatcgag atcagcaacc agtgcgcacg
    18361 gctgatcgcc aacacgatca ttttctacaa ctcggcaatc ttgtcgcggc tggtgaccaa
    18421 gtacgaggcg gccggcaaca gcaaggcgct ggcactcatc acaaaaattt cgcctgcggc
    18481 atggcggcac atcctgctca acgggcacta cacctttcaa agcagcggca agacgatcga
    18541 tctcgatgcg atcgtcgcag gcctggaact ggagtgacgg aattttcagc ggttccgtgt
    18601 tagcagcagt gtaaaactga cccaccccag cgaagtaaaa ctgatccacc tgggctagtt
    18661 tggcggcctt ttcgaggccg tcgatgttga cccaggagca aaccgtggaa atcagagttc
    18721 ttgcccgaca gggccactcc atccggcaca tcgcccgcac gttgggggtc tcgcgcaata
    18781 ccgtgcgccg ctacctccgc gatccgtcgg tagcgcgata ccagccccgt gaaccacgcc
    18841 ccaccaaact ggggccattt gaatcttatc tgcggcaacg ggtcgagcag gcccacccca
    18901 tctggctgcc tgccaccgtc ctgaatcggg aaatccgcgc tcaagggtat ggcggcggtc
    18961 tctcgttatt gcgcgccttc ctcgccacgc tcaagcccgc ccgccgcgaa gcaggtccgg
    19021 ccgtccgttt cgagaccgag cccggacggc agttgcaggc tgatttcgtg gtcttgcgcc
    19081 gggccagatc ccccatgtcg gccttcgtcg ccacgctggg atacagccgc atgacgtttg
    19141 tcaccttcgt accggacgaa tccttcgagt ccgtgcggga ctccctgttg ctggcttttg
    19201 actatctggg cggtgtgccc cgcgaggtgt tgttcgacaa catgaagacc gtggtgctgg
    19261 aacgcgatgc ctacggtgac ggcaagcacc gttttcaccc cggcttgctt caactggccg
    19321 atgacctggg gttccggatc cggctgtgtc gcccgtatcg ggcccagacc aaaggcaagg
    19381 tcgagcgctt caatcgctac ttccgcgaga gcttctacaa cccgttgctg acccggatga
    19441 agggcacggg gctgctgctc gattgtgctg ctgccaaccg acgcgtccgt gactggttgg
    19501 cagacgaggc gaatgtgcgg gtccacgcca ccctgaacga gcgccccatc gaccgttggc
    19561 ggcaggagcg ggaacacctg caacccttgc catcccgtgt gcggcgcgac gaggcccccc
    19621 tgctggataa cagtctccgc ccggtaccac tggaatctct ccagcaccca ctgtctgtct
    19681 atgacgcaat tggggaggcc tgccg
//
