LOCUS       NZ_CP145178             9383 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain CAVp50 plasmid pCAVP50-9383, complete
            sequence.
ACCESSION   NZ_CP145178
VERSION     NZ_CP145178.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09205472
            Assembly: GCF_036787225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Tn5403 promotes plasmid rearrangements and degradation of the
            Klebsiella pneumoniae carbapenemase (KPC) transposon Tn4401
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2024) Biological Sciences, University of Adelaide,
            Victoria Drive, Adelaide 5005, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x and 104x
            Sequencing Technology             :: PacBio and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036787225.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 16:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,608
            CDSs (total)                      :: 5,483
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 67 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 20 of 163
            Pseudo Genes (multiple problems)  :: 19 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CAVp50"
                     /isolation_source="Peri-rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCAVP50-9383"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2014-07-01"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(119..232)
                     /locus_tag="DKD22_RS27990"
                     /old_locus_tag="DKD22_27990"
     CDS             complement(119..232)
                     /locus_tag="DKD22_RS27990"
                     /old_locus_tag="DKD22_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_223178531.1"
                     /translation="MQEPPFQPYCCACSGNCSLGSNPERHDKSPLAAATGN"
                     /name="replication initiation protein"
     gene            888..1037
                     /locus_tag="DKD22_RS27995"
                     /old_locus_tag="DKD22_27995"
     CDS             888..1037
                     /locus_tag="DKD22_RS27995"
                     /old_locus_tag="DKD22_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_187416765.1"
                     /translation="MPSEHDEQRDATDTQLPGEYDLFDLMAQITEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1517..>1585
                     /locus_tag="DKD22_RS28000"
                     /old_locus_tag="DKD22_28000"
                     /pseudo=""
     CDS             1517..>1585
                     /locus_tag="DKD22_RS28000"
                     /old_locus_tag="DKD22_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1619..4516)
                     /locus_tag="DKD22_RS28005"
                     /old_locus_tag="DKD22_28005"
     CDS             complement(1619..4516)
                     /locus_tag="DKD22_RS28005"
                     /old_locus_tag="DKD22_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_064175833.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDIQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4611..5216
                     /locus_tag="DKD22_RS28010"
                     /old_locus_tag="DKD22_28010"
     CDS             4611..5216
                     /locus_tag="DKD22_RS28010"
                     /old_locus_tag="DKD22_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            <5237..5377
                     /locus_tag="DKD22_RS28015"
                     /old_locus_tag="DKD22_28015"
                     /pseudo=""
     CDS             <5237..5377
                     /locus_tag="DKD22_RS28015"
                     /old_locus_tag="DKD22_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5377..5778
                     /locus_tag="DKD22_RS28020"
                     /old_locus_tag="DKD22_28020"
     CDS             5377..5778
                     /locus_tag="DKD22_RS28020"
                     /old_locus_tag="DKD22_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644821.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHLSGQVSEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6518..6763)
                     /locus_tag="DKD22_RS28025"
                     /old_locus_tag="DKD22_28025"
     CDS             complement(6518..6763)
                     /locus_tag="DKD22_RS28025"
                     /old_locus_tag="DKD22_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023284667.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKSLPKAVKQQVRERV
                     LRAIQDERESRLAKKMRREKYEAAEDTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7090..8775
                     /locus_tag="DKD22_RS28030"
                     /old_locus_tag="DKD22_28030"
     CDS             7090..8775
                     /locus_tag="DKD22_RS28030"
                     /old_locus_tag="DKD22_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_023284666.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPNMMSK
                     IMTSLPADTVTETPVSTLPLEQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNGRITSTEKTIPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWVGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8785..9042
                     /locus_tag="DKD22_RS28035"
                     /old_locus_tag="DKD22_28035"
     CDS             8785..9042
                     /locus_tag="DKD22_RS28035"
                     /old_locus_tag="DKD22_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_022644819.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9127..9276
                     /locus_tag="DKD22_RS28040"
                     /old_locus_tag="DKD22_28040"
     CDS             9127..9276
                     /locus_tag="DKD22_RS28040"
                     /old_locus_tag="DKD22_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_029497523.1"
                     /translation="MKKVKAIFLFILIVSGFLIVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2090..9383
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          680..895
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.258"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          131..316
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="6.160000000000001e-73"
ORIGIN
        1 gcaagtttct gaaccgtggt aacagtcttg tgcgagacat gtcaccaaat ctgtccttcc
       61 agtgtagccg cagtcaggcc accacttcaa gaactcttcg aaacatatct cgcacatttc
      121 agttaccagt ggctgctgcc agtggcgatt tatcgtgtct ttccgggttg gacccaagag
      181 aacagttacc ggaacaggcg cagcagtagg gctgaaaggg gggttcctgc atacagcccg
      241 gcccggagcg aactgcctaa gttggtccga gataccagaa gacgtgagct atgagaaagc
      301 gccacgtgtc ccgtaaggga gaaaggcgga aaggtatccg gtaaccgata tggcgggaac
      361 gtgggggcgc aggagggagc gattcaccgg aaacggtgat gatctttaag ccacgtcggg
      421 tttcgtcacc gctggcggat tcatggttga gccttacggc tcccacagat gcacaaaaaa
      481 agtgtctgtt atgtgaactc cgtcaggggg gcggagccta tggaaaaagc gcctgcggcg
      541 cggccttttc gtcctttctt tttttgcgca catgttctgt gatgcgttat cccctgattc
      601 tgtggataag gtatgagtct gttgtgtgag cgtaggcaac gcccgccgca gccgaacggc
      661 cggagcgtga gcgaccgagt gagcgaggag gcgtcatttc cccggaaccg gcattcagca
      721 cttacgcgtt ccggccacat ttttctcctg ccacatgaag cacttccctg gtaaccacgc
      781 tcatgccaac ataggaagcc agtacacact ccgctagcgc tacgtgactg gttcagggct
      841 gcgccccgaa acccgctaac aacctctcag ctactggata tcaccccatg ccatctgaac
      901 atgatgaaca acgggatgct actgatacgc agcttcccgg cgaatatgac ctttttgatt
      961 taatggcgca gattactgaa gagaacctgc atgcccctgt ggattctggg ccgcctgtcg
     1021 gccgggaagc gttatagctt tttcgcttgc ccgcgtacgt ggtaatacgc cggtattaca
     1081 ttttatttct ttattttcat gtaattacag gtgattgtaa tacgttgcgt attacataaa
     1141 taaacggaga tttcagtttt taccttttaa ttcatgcagt tgcgatgaaa atcgcggctg
     1201 cgttaggtgt atgaccattt aaggggtaaa aaattatcat tctggccgcg tgatctgcgc
     1261 aggttaagga gcatcggcga cgaaaaagcg tctttaactg cttcgttcat gcttgcatga
     1321 acggatgatt tttccagact acaggtaaac tacaggaaga ctacaaaagt tgatgattta
     1381 atactgattt ttagtgcgat gactacagat aaactacaaa ataactacag gattacctca
     1441 gatccgttct ctgaaccatt cgtacatccg acccccttac cggtttacac tgagcactga
     1501 tgctctgagg gcccttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
     1561 ccagaatcag tctctgctcc tgctgggggt cggttccggc tgagggcgaa atgacaccct
     1621 aagctttcgg ttccttgggc caaagatatt cgccagtcag tagaatgtgc gcccagccca
     1681 atggggatat gtggggaaga aattcagggg gaacatccaa cccttcgttc cgccgctccg
     1741 tgacggcatg accaagatgg acggtattcc agtaaatgat caccgcagtc aataaattga
     1801 gcccagcgat tcggtagtgc tgcccctctg tcgtgcgatc gcgaatttcc ccctgcctcc
     1861 cgatacggag cgcatttttg agcgcatggt gggcctctcc cttgttaaga ccgatctgag
     1921 cacgccgctg aatgtccgta tccaggatcc actcaataat gaaaagggtc cgttcaatac
     1981 gaccaacttc acgaagcgca actgcaaggt tgttttgtcg tgggtaagaa gcgagcttgc
     2041 gcaggagttg gctgggcctg attttgccag cggtcatcgt cgcggcacaa cggaaaatat
     2101 caggccagtt cgcaacgata agatcctccc gggcttttcc acctaccaac ttgcgtaact
     2161 ccctgggggt cgtatcggga ttaaatacgt acaaccgctt cgatggcaga tccctgattc
     2221 gcagaacgag attgtagccg agcaggctac tggctccgaa caaatggtcg gtgaatcctg
     2281 ctgtatcggc atactgttcg cgaacatggc gaccgacctc gttcatcagt agtccatcga
     2341 gaatatacgg tgcctcgctc acggtcgccg ggatcgactg acaagcgaat ggcgcgaact
     2401 ggtcgcttac gtgagtatac gctttgaggc cgggaacaga accatatttg gcattgacca
     2461 tgttcatggc ttcgccatgc cgcgctgtcg ggaaaaactg accatcgctc gatgctgacg
     2521 tgcccatccc ccagacgcgt gacatcggca gtttaccctg cgcggccacc acaattgcca
     2581 atgcctggtt catggcttcg ctttcaacat gccagcgggc aaggcgtgag agctgccagt
     2641 aatcatgcgt gtttgtagct tccgccatct tacgcaggcc cagattgagc ccttcagcga
     2701 gcaggacgtt gagcagaccg atccggtcgc gacatggagc cccggttctc agatgggtaa
     2761 acgcatctgt gaaaccaagg gctgcatcaa cttcaagcag catgtcggta atccgaacgg
     2821 acggcattcg gcgatacaga tccagtatga gtgcctcggc accatccggc acgtctgctg
     2881 tcaacctgtc gatccgcaac gttccatctt ctatgctacc gtgcggaata gtgccgttac
     2941 gggcagcccg ggccagccgc ttaagagcga tcgtgagtcg cgcctttctg tctgccagcc
     3001 aatcctgtgg gttggaaggc acggccagtt ttgcattttc ctgcgccgcg atcatcggca
     3061 ccagtacctg cttgaggtca ccatagcggc gcgaatgagc gagccagaca tctccggaac
     3121 gaaaagcatc ccggaggtga aagagtacag ccacttccca aagacgggta tctccttttt
     3181 cctgagctcg taaatgacgg ttccatttgg agctgggccg caggaaacgc ctttctggcg
     3241 atgcaacacc tttcatctct ccgatcgaca aagctgctgc taccaatggt ccggcgaccg
     3301 gcgcggcttc gagcttcaga cagcgcaaca tgcggggcgc ataacgacga aagcgatggt
     3361 atccctgccc gacatatgca agaggctcat cggctagcgt gttgctgagt tgagtccctg
     3421 tcgctaccag ttgagcgagc cggtcccatg caaccgaact ggcgacagcc atctccagcg
     3481 gggttccgtc actgcgggcc tcaagcaacg aagctcccag cgcggtgaag gtacggatcg
     3541 tatccgtgag tgtggcttta gagccggaaa ttgtttcgtc atgctggcgc ttcgcttccc
     3601 gccaggtttt tcctacgatc ctgtcatggg tttcgactat ggcatcagca atcgccgctt
     3661 cccactccac aacacagacg gcaaggatcg cccagcggcg gtccgaagtg atgtcacgca
     3721 aaccgtcggt gaagtagcgt tcaccctgcc gacgcagccg ggcaatgcga tgggcaggta
     3781 tgctggccaa agcactatga ttgatattca gggtacgcag aaattcgagc ctgtcgagca
     3841 aacggttagc agcagccgag ttgttaccaa cctcgaagtt gcgaagccag atgaaacgac
     3901 tgatattgcc ggcgagcatt tcactcagaa gtttgtccag gtgatcgcga acatccgctg
     3961 ttaaattttc cgcaatccgc gtttcaatcc gccgctcagc ggcgaccaga gcatccgcgc
     4021 acaagcgctc gattgtcgat actgcgggca gaatggtgga agtttcccga caccgcacaa
     4081 taaaacgatg agcaagatcc tcgtttgatc tggcatcttc ggcctggccg aaagtccact
     4141 cccgcagatc acgggcacca cggcccgtga aggtcttgta gccgtaaatt tcgcgcagcg
     4201 tgtccatgtg ctgctgacgg gtttggcgcc gtgtggcata agtgagaagc gcatcagccg
     4261 gaactccaag ctgagcaccg acgaaggaaa ggacttcacg cgggatcatc tcaccaggag
     4321 ccagtgcacg gcccggatat cgtaaggcac aaagttgcag ggcaaagcca atcctgtttt
     4381 ccggtctgcg gcgctgccta atgttttcca ggtcatcatc gcccagcgtg tagaacttca
     4441 gtagcgacag ttcgtccgtg ggcagatcga acagcgctgc tcgctgccgt tcggtgaaaa
     4501 tatggcgtcg tgacatacaa attcgtccct tttgaagtat agtctgtttt ggacaacagc
     4561 cagcccatat aaatcagggc gttccgatac aaaaatccag gagggttcaa ttgggacatc
     4621 gtgccgccat ttactgccgg gtttcaacag cggatcagtc ttgtgaacgc caggaatttg
     4681 atctgcgagc cttcgccggc cgtgccggct acgacgtggt gggaatattt aaggaaacag
     4741 gttcaggaac taaactcgac cgggccgagc gaaagaaagt cctggcgctt gcccagtcca
     4801 gacaaattga tgcaatcctg gtcactgagc tttcccggtg ggggcgctcg acgctcgatc
     4861 tgctcaatac gctacgtgaa ctggagaact ggaaggtttc cgtgatagcc atgaatggaa
     4921 tggcgttcga tctttcgtcg ccgtatggac gaatgctggc gacgtttctt tccggcattg
     4981 cggagtttga gcgggatctc atcagcgagc gggtcaagtc aggccttgct gttgcgaagg
     5041 cacgtggtaa gaggcttggt cgtcaggccg gagtgcgacc aaaatcagac cgacttttgc
     5101 ctaaggtggt tgctatgagg gccgagggac gcagctatcg ctggatcgca cgcgagctcg
     5161 gtatcagcaa gaataccgtc gctgacatcg tgcaacgaca cagagctaac gcttagggtg
     5221 tcatttcgcc ctcagccgga accgacccct gctggagaag cttaacgagc ttgatctcga
     5281 tgttgaggcg gacctttgtg agaaactcca cgatgatgcg gaacatcttt tccgtacact
     5341 gtcctcgaga ctgaatgcat cacaggatgg taactgatgg ccagagtatc tattagtgag
     5401 gctgccagac tgacgggtaa gagtagaaca acgctgcaca ggttaattaa agcaggtgac
     5461 ttgtccacct gtagcggtga acgtaatgca aaaatggtag atacttcaga gcttttgcgt
     5521 gtcttcggtc cctttgagca acctaagggt gaacaccttt ctggacaggt ctctgaacag
     5581 cgtgatacag cctctgctga ccagaatgaa caggtgattc gtcatctgaa acaggagatt
     5641 gaacacctga acaccttact tttgtccaag gattcgcaca ttgacagcct gaaacaggcc
     5701 atgctccttc ttgaaaatca ccaggcaaaa ttaactgaat ccccggcgcc atggtggaaa
     5761 ttctggaaga aatcctgatc ctgccatggt tgagttttcc tctccggcca ttccggggag
     5821 ttcctgaacc ttcccagata tcgccagatc cgggaacact cctcaccacg aaaaataccc
     5881 acacaccacg caaaattaaa attttgcaga taactcctta ttatcagttg gttaggtttt
     5941 attaaattac gctgtattac cttctgtaag gtaaatccaa gtcgtcggcg attcgcgtac
     6001 gacttgccga cgactcgtca tagagtcatc gacgactcac tccatactat cagtaactta
     6061 tgacgacttg actctagagt tgagtcgttc atttggtgtt tttttgacct tggtgactct
     6121 agagtcaagt cacgagtcga atcgccggtg aatcgtgtgc taagtcgtcc ggtgacttgc
     6181 catcacgtca ttgccggctg tcgctgaacc ggattcctga acgtgctgaa aacgggtttt
     6241 ccggctgcgc cggcaacgcc tcacggccac gcaataaatt gcgtccccgt tcaccgtttt
     6301 cgggtaagaa gaacatcgga aacaggactt accccgactg aatggcgtga atttccgcgc
     6361 tatgcacttg cgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg
     6421 gttttcgggt ggtttgttgc ctgttttgcc ggttttccgt cagaaacgcg ctgagggtgc
     6481 ctgaggcggt gcgggcaacg gatgttatgg taaatcgtca acgccggcgc tgcggctgcc
     6541 aggtgaaggt gtcttccgcc gcctcatact tctcccggcg cattttcttc gccagcctgg
     6601 attcacgttc gtcctgaatg gcgcgaagca ccctttcccg cacctgctgt tttaccgcct
     6661 ttggcagtga tttattccct ttcaggccca tggccagcgc ctgggcgaca aaggcgtccg
     6721 ccagacgctt ctcttcagcg ggggtgagtt tcaggtgtgt catggtggcc tccgggtgtc
     6781 tctgggttcc tgaacagctt aattggtagt cctctcctca ttccagtccg ctttcagata
     6841 tgcatgtact tacataaaag caagtgtgat gaatatcgca aataatgaaa tttgttgccg
     6901 ggggaaactt tcgggaaaag acgcaacctg ctgacaggcg gctctggccg cttttttgtg
     6961 cctgaagatg agaactaatc tcatatattg acacctgaaa acagcaaggg taaggtaata
     7021 attatactgt tcatatatac agtatattgg ttttaattta ttgttttaaa tgtcaaagag
     7081 gataacgata tgagtggcgg agacggacga ggtccgggta attcaggtct gggacacaat
     7141 ggtggtcagg ccagtggaaa tgtgaacggt acgtcaggta aaggtggccc ttcatctggt
     7201 ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacacc taacggggat
     7261 attcataact ataacccggg tgagtttggt aacggcggga gtaaacccgg tggtaatggc
     7321 ggtaacagcg gcaatcatag cgggagctct ggtggcggac agtcttcagc caccgcgatg
     7381 gccttcggtc tgccagccct tgccactccg ggggctgagg gactggcttt atccgtctct
     7441 ggcgatgcgt tgtcggccgc tgttgctgat gtgctggccg ccctgaaagg tccgtttaag
     7501 tttggtctgt gggggatagc gatctacggt gtgctgcctt ctgagattgc aaaagatgat
     7561 ccgaacatga tgtcaaaaat tatgacgtca ttaccggccg atacggtgac ggagactccg
     7621 gtaagtactt taccactgga gcaggcgacg gttcgtgtca gacaacgggt tgtggatgtg
     7681 gtgaaggatg agcggcagca tattgcggtt gtcgcgggtc ggccaatgag tgttcctgtg
     7741 gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg
     7801 tctctgcagg tgagcgtacc gaaaggtgtt ccggccgcga aagccccgcc aaaaggcatt
     7861 gttgctgaaa aaggtgattc acgtccggct ggttttacgg ccggtggtaa ctcccgtgag
     7921 gccgttattc gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat
     7981 gttcttaccc cggcacaggt aaagcagcgt caggaggaag aaaagcgtcg tcagcaggca
     8041 tgggacgccg ctcacccgga agaggggctg aaaagagagt atgataaagc gaaagctgag
     8101 ctggatgccg aagataaaaa tattgcgacc ttaaacggta ggattacatc gacagaaaaa
     8161 accattcctg gtgcaagaac tgctgttcag gaagccgata aaaaggtgaa agaagccgag
     8221 gcaaataaag atgattttgt gacttacaac ccgcctcatg aatatggctc cgggtggcag
     8281 gaccaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcagct
     8341 caggcatctt taaacgcaat gaatgaatcc ttatccagag ataaggctgc gctttccggg
     8401 gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taaattaaat
     8461 gaggaaaaga aaaaacctcg caagggaact aaagattacg gccatgatta tcatccagcc
     8521 ccgaaaactg aagacataaa ggggctaggt gacctcaaaa aaggtacacc taaaacacca
     8581 atgcagggag gtggaggtag gcgtaaacgc tgggttggtg ataaaggtcg taagatttat
     8641 gaatgggatt cccagcacgg tgagcttgaa gggtaccgcg ccagtgatgg cgaacacctc
     8701 ggcgcattcg atccaaaaac gggtaagcag gttaaagggc cggatccaaa aagaaacatt
     8761 aaaaaatatc tttgagaggt aagtatggga cttaaattag atttaacctg gtttgataag
     8821 aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgatga tggttctgtc
     8881 attgaaagtc tggggatgcc tttaaaggat aatattaaca atggttgttt tgatgtgaaa
     8941 aatgagtggg tttcattatt gcaaccctac tttaaacata aaatcaatct ttctgatagt
     9001 tcatattttg tttcatttga ttatcgggat ggcaactggt aataaaaatt tatcaaggat
     9061 gagttgaaat acagacttcc ggtattcagg gatgaatgct ggagtcttta aatacaggga
     9121 aagtatatga aaaaggtaaa ggctattttt ctttttatat tgatagtttc tggctttctt
     9181 attgtcgcat gtcaggcaaa ctatatcagg gatgttcagg gcgggacggt ggcaccatca
     9241 tcctcctctg agctgacggg aattgcggtt cagtagaaaa gatcaaagga tcttcttgag
     9301 atcctttttt tctgcgcgta atctgctgcc tgtaaacgaa aaaaccaccc ggcaaggtgg
     9361 tttttcgaag gttcagcaaa ccg
//
