LOCUS       NZ_CP145177            12072 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain CAVp50 plasmid pKPC_CAVP50, complete
            sequence.
ACCESSION   NZ_CP145177
VERSION     NZ_CP145177.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09205472
            Assembly: GCF_036787225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Tn5403 promotes plasmid rearrangements and degradation of the
            Klebsiella pneumoniae carbapenemase (KPC) transposon Tn4401
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2024) Biological Sciences, University of Adelaide,
            Victoria Drive, Adelaide 5005, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133x and 104x
            Sequencing Technology             :: PacBio and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036787225.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 16:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,608
            CDSs (total)                      :: 5,483
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 67 of 163
            Pseudo Genes (incomplete)         :: 96 of 163
            Pseudo Genes (internal stop)      :: 20 of 163
            Pseudo Genes (multiple problems)  :: 19 of 163
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145177.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12072
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CAVp50"
                     /isolation_source="Peri-rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPC_CAVP50"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2014-07-01"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            join(11051..12072,1..4)
                     /gene="istA"
                     /locus_tag="DKD22_RS27955"
                     /old_locus_tag="DKD22_27955"
     CDS             join(11051..12072,1..4)
                     /gene="istA"
                     /locus_tag="DKD22_RS27955"
                     /old_locus_tag="DKD22_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="WP_004199214.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            1..780
                     /gene="istB"
                     /locus_tag="DKD22_RS27960"
                     /old_locus_tag="DKD22_27960"
     CDS             1..780
                     /gene="istB"
                     /locus_tag="DKD22_RS27960"
                     /old_locus_tag="DKD22_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="WP_004152394.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            1167..2048
                     /locus_tag="DKD22_RS27965"
                     /old_locus_tag="DKD22_27965"
     CDS             1167..2048
                     /locus_tag="DKD22_RS27965"
                     /old_locus_tag="DKD22_27965"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="WP_004152396.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     gene            complement(2298..>3263)
                     /locus_tag="DKD22_RS27970"
                     /old_locus_tag="DKD22_27970"
                     /pseudo=""
     CDS             complement(2298..>3263)
                     /locus_tag="DKD22_RS27970"
                     /old_locus_tag="DKD22_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(3297..>6038)
                     /locus_tag="DKD22_RS27975"
                     /old_locus_tag="DKD22_27975"
                     /pseudo=""
     CDS             complement(3297..>6038)
                     /locus_tag="DKD22_RS27975"
                     /old_locus_tag="DKD22_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(6090..7805)
                     /gene="tnpR"
                     /locus_tag="DKD22_RS27980"
                     /old_locus_tag="DKD22_27980"
     CDS             complement(6090..7805)
                     /gene="tnpR"
                     /locus_tag="DKD22_RS27980"
                     /old_locus_tag="DKD22_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="WP_004152391.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            7915..10944
                     /gene="tnpA"
                     /locus_tag="DKD22_RS27985"
                     /old_locus_tag="DKD22_27985"
     CDS             7915..10944
                     /gene="tnpA"
                     /locus_tag="DKD22_RS27985"
                     /old_locus_tag="DKD22_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="WP_004152392.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     ARG             1168..2045
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 atgaacctcc agcatgagcg gatcgacgcc ctgtgccagc aactcaaact cgaacgggtg
       61 gcaacgcatt accccgtcct ggcgcagcag gcggtgcgcg aggacttcag cttcaccgac
      121 ttcctggaac acctgttgcg gcatgaagcg gggtgtcgcc agcagcgcag ccgcgaactg
      181 ctgacgcgga tggcttgctt cccgggcatc aagaccctgg aggactatga cttcagcttc
      241 aaccccggcg tgcccaaggc actggtgcag gagctgggca gtctggcgtt tgtcgaacgg
      301 gccgagaacg tggtgctgat cggaccctct ggcatcggca agacccacct cgccatcgcc
      361 ttgggttaca aggccgcgca agccggggtc aagacacgct tcatcacggc agccgacctg
      421 atgctgcaac tgagcacggc gcagcgacaa gggcggctga aggaatactt caaccgcgcc
      481 atcctcggtc cacggctgct ggtgatcgat gagatcggct acctgccttt cggacgcgag
      541 gaagcgaacc tgttctttca ggtggtggcg aagcgctatg agaaaggggc gatgatcctg
      601 acctcgaacc tgccgttcag ccagtgggcc gacgcgtttg ccggcgatac caccctgaca
      661 gccgcgatgc tggatcggct gctgcaccat gcccatatcc tgaccctgag cggcgaaagc
      721 taccgcttga aggacaagag gaaggcggga gtggtcagga aaaattccaa acccgaatga
      781 tccaggtggg tcagtattac tttggtgatt caggggtaaa gtgggtcagt tttcagttgg
      841 tgttgacacc ggcgtaccct cggtgctatc ttcgcgcccc aatagtcggg gcttggccag
      901 gacttcctga ggccgtccgt aacgtggatg ccgaggtcag gcgaggtggc cgacccatga
      961 acgccgacct gattcgtttt tcaatagcgc tggacgttgt ggtgccaggg acttaccaac
     1021 ccgatgtgtg cccatccggg gcagttacag ccgttacagc ctctggagag ggagcggctt
     1081 gccgctcggt gataatccca gctgtagcgg cctgattaca tccggccgct acacctagct
     1141 ccaccttcaa acaaggaata tcgttgatgt cactgtatcg ccgtctagtt ctgctgtctt
     1201 gtctctcatg gccgctggct ggcttttctg ccaccgcgct gaccaacctc gtcgcggaac
     1261 cattcgctaa actcgaacag gactttggcg gctccatcgg tgtgtacgcg atggataccg
     1321 gctcaggcgc aactgtaagt taccgcgctg aggagcgctt cccactgtgc agctcattca
     1381 agggctttct tgctgccgct gtgctggctc gcagccagca gcaggccggc ttgctggaca
     1441 cacccatccg ttacggcaaa aatgcgctgg ttccgtggtc acccatctcg gaaaaatatc
     1501 tgacaacagg catgacggtg gcggagctgt ccgcggccgc cgtgcaatac agtgataacg
     1561 ccgccgccaa tttgttgctg aaggagttgg gcggcccggc cgggctgacg gccttcatgc
     1621 gctctatcgg cgataccacg ttccgtctgg accgctggga gctggagctg aactccgcca
     1681 tcccaggcga tgcgcgcgat acctcatcgc cgcgcgccgt gacggaaagc ttacaaaaac
     1741 tgacactggg ctctgcactg gctgcgccgc agcggcagca gtttgttgat tggctaaagg
     1801 gaaacacgac cggcaaccac cgcatccgcg cggcggtgcc ggcagactgg gcagtcggag
     1861 acaaaaccgg aacctgcgga gtgtatggca cggcaaatga ctatgccgtc gtctggccca
     1921 ctgggcgcgc acctattgtg ttggccgtct acacccgggc gcctaacaag gatgacaagt
     1981 acagcgaggc cgtcatcgcc gctgcggcta gactcgcgct cgagggattg ggcgtcaacg
     2041 ggcagtaagg ctctgaaaat catctattgg cccaccaccg ccgcccttgc gggcggcatg
     2101 gattaccaac cactgtcaca tttaggctag gagtctgcgc ggcagagccg tgtgaccggt
     2161 tttctgtaga gcactgacga tggcggcggc gctctctgca attggcaagg cgtcggcgcc
     2221 aaggatacca atcttgcggc gcgcggcgtg ttatgacgac tggggtgcat ttgagccgcc
     2281 ccatttaacc ttcgccctca cagatacgcc attcgcctca gatttagcgc catgcagacg
     2341 agcttccact cggcttgcac cttgtccagg cccctcatgc tgaactgacg caatcccatc
     2401 accgccttga tccaaccatt cggagcctcc acgatcgact tgcgccggcg gtaagctgca
     2461 tcgccttgct ccgttttcaa tttcgccgca atcgccgccg tatgcggatg ggtcttggca
     2521 ttgaccttgg catcttcacg tccctcgcgg ccgagggcaa cgatgacatc gccgtggtga
     2581 tcggcgacct ttgccagaac agcctcacta cggaatcccg catccgccag cgtctgggcc
     2641 ggcatttctc cggtgttggc ctgaactgct gccagcatgc ccagcagcgc ctgactgtcc
     2701 gcggcgcagt tggtcaactc cgccgccacg atgatctggt gctcggcatc gaccgctgtg
     2761 tacccgttgt agctctgctc ggagccacca ccggcgtgtt tcatgatccg gctgtccgga
     2821 tcggtgaagc tttcctgatc acggtcatcc ggcacaccaa actcgcgttt gtacgagcca
     2881 ccgcccttgt ccgagccatc cggatggcga ggccggcggc catcgtcttc gctgcgcccc
     2941 cgggcctggt ccgcttcacg ctggcgcgct tccaggcgcg cctttgccgc ctggatcgcc
     3001 tccaggcgct tctcgcggcg agaaatctcg gcaggaatgt ccagctccgg ctcgttacgc
     3061 tcctggtcgt cggtagcctt ggcgcgatca agcagcgcct tgatctcgca atgcaattcg
     3121 tcctcggccg gcttcatgcg cttatagctc atcgccttgt ggcggctggc gttggctttt
     3181 accttggtgc cgtcgaccgc gatcgtgcca agcttcacca gcccacattc gcgcgccagt
     3241 tgcaccacct gaacgaacaa attggggtcg gttccggctg agggcgaaat gacaccctaa
     3301 gctttcggtt ccttgggcca aagatattcg ccagtcagta gaatgtgcgc ccagcccaat
     3361 ggggatatgt ggggaagaaa ttcaggggga acatccaacc cttcgttccg ccgctccgtg
     3421 acggcatgac caagatggac ggtattccag taaatgatca ccgcagtcaa taaattgagc
     3481 ccagcgattc ggtagtgctg cccctctgtc gtgcgatcgc gaatttcccc ctgcctcccg
     3541 atacggagcg catttttgag cgcatggtgg gcctctccct tgttaagacc gatctgagca
     3601 cgccgctgaa tgtccgtatc caggatccac tcaataatga aaagggtccg ttcaatacga
     3661 ccaacttcac gaagcgcaac tgcaaggttg ttttgtcgtg ggtaagaagc gagcttgcgc
     3721 aggagttggc tgggcctgat tttgccagcg gtcatcgtcg cggcacaacg gaaaatatca
     3781 ggccagttcg caacgataag atcctcccgg gcttttccac ctaccaactt gcgtaactcc
     3841 ctgggggtcg tatcgggatt aaatacgtac aaccgcttcg atggcagatc cctgattcgc
     3901 agaacgagat tgtagccgag caggctactg gctccgaaca aatggtcggt gaatcctgct
     3961 gtatcggcat actgttcgcg aacatggcga ccgacctcgt tcatcagtag tccatcgaga
     4021 atatacggtg cctcgctcac ggtcgccggg atcgactgac aagcgaatgg cgcgaactgg
     4081 tcgcttacgt gagtatacgc tttgaggccg ggaacagaac catatttggc attgaccatg
     4141 ttcatggctt cgccatgccg cgctgtcggg aaaaactgac catcgctcga tgctgacgtg
     4201 cccatccccc agacgcgtga catcggcagt ttaccctgcg cggccaccac aattgccaat
     4261 gcctggttca tggcttcgct ttcaacatgc cagcgggcaa ggcgtgagag ctgccagtaa
     4321 tcatgcgtgt ttgtagcttc cgccatctta cgcaggccca gattgagccc ttcagcgagc
     4381 aggacgttga gcagaccgat ccggtcgcga catggagccc cggttctcag atgggtaaac
     4441 gcatctgtga aaccaagggc tgcatcaact tcaagcagca tgtcggtaat ccgaacggac
     4501 ggcattcggc gatacagatc cagtatgagt gcctcggcac catccggcac gtctgctgtc
     4561 aacctgtcga tccgcaacgt tccatcttct atgctaccgt gcggaatagt gccgttacgg
     4621 gcagcccggg ccagccgctt aagagcgatc gtgagtcgcg cctttctgtc tgccagccaa
     4681 tcctgtgggt tggaaggcac ggccagtttt gcattttcct gcgccgcgat catcggcacc
     4741 agtacctgct tgaggtcacc atagcggcgc gaatgagcga gccagacatc tccggaacga
     4801 aaagcatccc ggaggtgaaa gagtacagcc acttcccaaa gacgggtatc tcctttttcc
     4861 tgagctcgta aatgacggtt ccatttggag ctgggccgca ggaaacgcct ttctggcgat
     4921 gcaacacctt tcatctctcc gatcgacaaa gctgctgcta ccaatggtcc ggcgaccggc
     4981 gcggcttcga gcttcagaca gcgcaacatg cggggcgcat aacgacgaaa gcgatggtat
     5041 ccctgcccga catatgcaag aggctcatcg gctagcgtgt tgctgagttg agtccctgtc
     5101 gctaccagtt gagcgagccg gtcccatgca accgaactgg cgacagccat ctccagcggg
     5161 gttccgtcac tgcgggcctc aagcaacgaa gctcccagcg cggtgaaggt acggatcgta
     5221 tccgtgagtg tggctttaga gccggaaatt gtttcgtcat gctggcgctt cgcttcccgc
     5281 caggtttttc ctacgatcct gtcatgggtt tcgactatgg catcagcaat cgccgcttcc
     5341 cactccacaa cacagacggc aaggatcgcc cagcggcggt ccgaagtgat gtcacgcaaa
     5401 ccgtcggtga agtagcgttc accctgccga cgcagccggg caatgcgatg ggcaggtatg
     5461 ctggccaaag cactatgatt gatattcagg gtacgcagaa attcgagcct gtcgagcaaa
     5521 cggttagcag cagccgagtt gttaccaacc tcgaagttgc gaagccagat gaaacgactg
     5581 atattgccgg cgagcatttc actcagaagt ttgtccaggt gatcgcgaac atccgctgtt
     5641 aaattttccg caatccgcgt ttcaatccgc cgctcagcgg cgaccagagc atccgcgcac
     5701 aagcgctcga ttgtcgatac tgcgggcaga atggtggaag tttcccgaca ccgcacaata
     5761 aaacgatgag caagatcctc gtttgatctg gcatcttcgg cctggccgaa agtccactcc
     5821 cgcagatcac gggcaccacg gcccgtgaag gtcttgtagc cgtaaatttc gcgcagcgtg
     5881 tccatgtgct gctgacgggt ttggcgccgt gtggcataag tgagaagcgc atcagccgga
     5941 actccaagct gagcaccgac gaaggaaagg acttcacgcg ggatcatctc accaggagcc
     6001 agtgcacggc ccggatatcg taaggcacaa agttgcaggg gttctaagcg ggaatcccag
     6061 aaaattccgt cattccgtat gatgaaaatt cagtcgactc ctcgcttgac gtgacgagcg
     6121 ccaaaagcct gccgcatctg cctggcgcgc tcgagctcat cgccatgcaa atacatcgac
     6181 gtcgtggcga tcgacgcatg gcgtaggttg tcgcgcaccg cggtgagctg agctccccgg
     6241 gccaacgcat ggctggcatg actgtgacgc atccaatgag gcgtggcacg gcgcagcttt
     6301 tcggcggcga ccggatgatc ggcctcaatg gcctcagcgg cttgcgtaaa gaatcttcgc
     6361 aacacgcacc aaaggtgagg gccggtgatg ccggcattac cgtcttcccc aagactgccc
     6421 accaggggcg ttttcggatt ccaccgttcc cggctgactg gcagttgacg ctgtgccaag
     6481 tattggtcca gcgccgtata cgcgagcggc ggcaaggcca ccttacctgg tttgccacct
     6541 ttgccaacca aatgcagcca gtggtcaccg tgctcgtcga ggcggacatt acccaggacg
     6601 gcgccgacca gttcgctagc gcgcaggccc gtggcatagc cgaagtcgag catgaagcgc
     6661 aggcgttgtg ctgccggctc ggaccagccg taggaccatt ccaggccgtc tgcaatggcg
     6721 cgcagcagta gccattctcc ctcggtaaag cctcgcgccg tatccagtgc gggccgcaag
     6781 gcgtgaccgc gtaccttgac gccggcgaaa gggttggcca gcacatagcg ctgctcgatg
     6841 agccaccgga acagcgccga gagcaccgtc agcgcatacg ctgccgagcg tgcggacaag
     6901 ccgcccgaga aggggcgcca ttccaccgaa tcgcgtggtc gaggtggccc cacccaacgc
     6961 tcgcgcgggg tcgggtgccg gacaaaggcc cggtacgcga ttgcatcgtc cgtggtcagg
     7021 gatgatagcg cccgtccgcg ttcgacgatg gcccacagga ttagccgcgc agcctccttg
     7081 cgataggcgc gctgtgtggc gacagtctcg tgcagcgcca gccaggcctg cacggcctcg
     7141 tagtcgttgg tcgcattcag cgtgcaagcg ttgcgcgggg cccggaaatt gccctgcgag
     7201 ccgtcgactt cgttcggcag acgcaactgc tcccacggca cgatgccgct gggctgaata
     7261 atcgggatca gggcccgggc gcgctcggtg agctgcggat gtgcggcaaa gaaagcctcg
     7321 acctgccggg cgcctgtcat gccgagtccc tcgatggctt tccaccagcc acgccggcgg
     7381 ggaatgcgca cggtcagcgc cgagagggtg tggataccgt acgcctgcag tgccctgacg
     7441 gtcctcggag acagccactg gtcgatgctg tcgctgatct ggggcttcgg cgccggcagc
     7501 gtgggcaaga gccggatcgc gcgttcgacg gcacccgcgc gcccctgtcg ctcttcgggg
     7561 gcgtgctgga acacgtcggc cagatccggc cggtggcggc tgttggcgaa aacgatgagc
     7621 tggcggcgga tatgccccag catccctcgc gctgattgac cggcggcctt gcgtgccgcc
     7681 aggtagtgtt caacggccgc gcgagagctg agtcccgcgt accagccgcg caaggcagcc
     7741 agcgaatccg catcgggaaa gccgactggg gtagaactcg aagccgtagt ctggcccgtt
     7801 ttcatatgga caggttgcga ttcgaaaagc gagtaacgcg ataataacga ttatttcagt
     7861 agaatcgaaa atgccccatg tttctacgaa acctcaagat agcaagaacg ggccatgagc
     7921 aagaagaaca agctactcag cgtcctatcc gacgccgaac aggaggcctt gtatggcctg
     7981 ccggagttcg atgatgcgca gcagctggaa tatctggctg tgacagaaac cgagttggcg
     8041 cttgccaaca gccggcccag tcttcatgcc aaggtctatt gcctcttgca gatcggctac
     8101 ttcaaggcca agcatgcctt cttcagtttc gactgggacg aagtcgagga cgattgcgct
     8161 ttcgtgctga gccgctattt ccacggcgag gtgttcgaac ccaaggcgat caccaagcat
     8221 gaacgctaca cccagcgcga gcagatcgcc aaactattcg gctactggcc gtggtcggcc
     8281 gccttcctgc cgcagctcga gcagcaggcc gcacggaccg tgcggcgtga cgtaacgccg
     8341 gggttcgtcg ccgccgagct gatcgtctgg ctcaatgagc acaagattat ccggcccggc
     8401 tatacgacct tgcaagagtt ggtcagcgaa acgctatccg ccgagcgccg gcgcctgggc
     8461 aatctgctgg agcaagcgtt ggacgaatct gccaaggccg cgctgagcca gcttctggtc
     8521 cgtgatgaca ccctgtcgca actggcggcg ctcaagcagg acgccaagaa tttcggctgg
     8581 cgccagatgg tccgcgagcg cgaaaagcgc gccacgctgg agccgctgca cgcaatcgcc
     8641 aagacactgc tgcctaaact cgacgtctcg cagcagaacc tgctgtacta cgcgagcctg
     8701 gcaaacttct acaccgtcca cgacctgcgc aatctgaagg ccgatcagac ccaactctac
     8761 ctgctgtgct atgcctgggt acgctaccgg cagctctccg acaacctggt cgatgcgatg
     8821 gcgtaccaca tgaagcagtt ggaggacgaa agcagcgctg gtgccaaaca atcctttgcc
     8881 gacgagcagg tgcgccgtca gcaggaaacg ccgcgggtag gccgcctgct gtcactctac
     8941 gttgatgata gcgtggccga tcccacgccc ttcggcgagg tgcggcagcg cgcctacaag
     9001 atcatgcctc gcgatacgct gcaaaccacg gcgcagcgta tgagcgtgaa gccggcgagc
     9061 aagctggcct tgcagtggca ggcggtggac gggctggccg accgcatgcg ccgccatctt
     9121 cgtccactgt acgttgcgct cgacctctcc ggctccaatc cagacagtcc atggcttgcc
     9181 gcgctggcct gggccaaagg tgtttttgcc aagcagcagc ggctgtcgca acggccactc
     9241 gacgagtggc ccgcggcgac acttccgaaa cgcttgcggc cgtacctgct gaccttcgac
     9301 gccgacggta aaccaaccgg cctgcacgct gaccgctacg agttctggct gtaccgccag
     9361 atcaggaagc gtctccaatc gggtgaactc tacctcgacg acagcctgca acaccggcac
     9421 ttctcagatg aactggttga gatggacagg aagatcgatg tgctcgcaaa aatggagatc
     9481 cccttcctgc agcaacccgt ccatgcccaa ctcgatgcgc tgactgccga actgcgcacg
     9541 cagtggctgg cgttcaaccg cgagctgaaa cagggcaagc tgacgcacct cgaatacaac
     9601 aaggacacgc agaaactgac ctggcgcaag cccaagagcg agaatgagaa agcgcgcgaa
     9661 aaggcattct acgagaagct tccgttctgc gacgtggccg acgtgtttcg cttcgtcaac
     9721 gacgagtgca agtttctgtc ggcgttcacg cccatgcagc cgcgctacgc gaagaaggtc
     9781 gccgacgctg acagcctgat ggcggtcatc atcgcgcaag cgatgaacca cggcaaccac
     9841 gtcatggcgc gcaccagcga catcccgtac cacgtgctgg atagcggcta ccagcagtac
     9901 ctgcgccagg caacgctgca cgcggccaac gactgcatca gcaacgccat cgccacgcta
     9961 ccgatcttcc catactactc gttcgacctc gaaacgctgt acggtgccgt cgacgggcag
    10021 aaattcagcg tcgaacggcc gaccgtgaag gcgcgctatt cgcgcaagta cttcgggcgc
    10081 ggcaagggcg tggtcgccta cacgctgctg tgcaaccaca tcccgctcaa cggctacctg
    10141 atcggcgcgc acgactacga ggcccaccac gtgttcgaca tctggtatcg caacacgtcg
    10201 gacatcatgc cgaccgcgat caccggcgac atgcacagcg tcaacaaggc caacttcgcc
    10261 atcctgcatt ggtttggccg gcggttcgag ccgcgcttca ccgacctcaa cgcgcaattg
    10321 aaagaactct actgcgctga cgatccagca cagtatcagg cgtgcctgat tcggccagtc
    10381 gggaaaatcg actgcgatct catccatcgc gagaagccga acatcgaccg gatcgtcgcc
    10441 acactcgggc tgaaggaaat gacgcagggc acgctgatcc gcaagctgtg cacctacacc
    10501 acgacgaacc cgacgcggcg cgcgatcttc gagttcgaca agctcatccg cagcatctac
    10561 acgcttcgct acctgcgcga tccgcaactg gaacgcaacg tgcaccgctc gcaaaatcgc
    10621 ctcgagtcct accatcagct acgctcggcc attgcccagg tcggcggtaa gaaggaactg
    10681 accggtcgca ccgacattga aatcgagatc agcaaccagt gcgcacggct gatcgccaac
    10741 acgatcattt tctacaactc ggcaatcttg tcgcggctgg tgaccaagta cgaggcggcc
    10801 ggcaacagca aggcgctggc actcatcaca aaaatttcgc ctgcggcatg gcggcacatc
    10861 ctgctcaacg ggcactacac ctttcaaagc agcggcaaga cgatcgatct cgatgcgatc
    10921 gtcgcaggcc tggaactgga gtgacggaat tttcagcggt tccgtgttag cagcagtgta
    10981 aaactgaccc accccagcga agtaaaactg atccacctgg gctagtttgg cggccttttc
    11041 gaggccgtcg atgttgaccc aggagcaaac cgtggaaatc agagttcttg cccgacaggg
    11101 ccactccatc cggcacatcg cccgcacgtt gggggtctcg cgcaataccg tgcgccgcta
    11161 cctccgcgat ccgtcggtag cgcgatacca gccccgtgaa ccacgcccca ccaaactggg
    11221 gccatttgaa tcttatctgc ggcaacgggt cgagcaggcc caccccatct ggctgcctgc
    11281 caccgtcctg aatcgggaaa tccgcgctca agggtatggc ggcggtctct cgttattgcg
    11341 cgccttcctc gccacgctca agcccgcccg ccgcgaagca ggtccggccg tccgtttcga
    11401 gaccgagccc ggacggcagt tgcaggctga tttcgtggtc ttgcgccggg ccagatcccc
    11461 catgtcggcc ttcgtcgcca cgctgggata cagccgcatg acgtttgtca ccttcgtacc
    11521 ggacgaatcc ttcgagtccg tgcgggactc cctgttgctg gcttttgact atctgggcgg
    11581 tgtgccccgc gaggtgttgt tcgacaacat gaagaccgtg gtgctggaac gcgatgccta
    11641 cggtgacggc aagcaccgtt ttcaccccgg cttgcttcaa ctggccgatg acctggggtt
    11701 ccggatccgg ctgtgtcgcc cgtatcgggc ccagaccaaa ggcaaggtcg agcgcttcaa
    11761 tcgctacttc cgcgagagct tctacaaccc gttgctgacc cggatgaagg gcacggggct
    11821 gctgctcgat tgtgctgctg ccaaccgacg cgtccgtgac tggttggcag acgaggcgaa
    11881 tgtgcgggtc cacgccaccc tgaacgagcg ccccatcgac cgttggcggc aggagcggga
    11941 acacctgcaa cccttgccat cccgtgtgcg gcgcgacgag gcccccctgc tggataacag
    12001 tctccgcccg gtaccactgg aatctctcca gcacccactg tctgtctatg acgcaattgg
    12061 ggaggcctgc cg
//
