LOCUS       NZ_CP145160             8756 bp    DNA     circular CON 05-APR-2024
DEFINITION  Raoultella ornithinolytica strain CAVp363 plasmid pCAVP363-8756,
            complete sequence.
ACCESSION   NZ_CP145160
VERSION     NZ_CP145160.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22837213
            Assembly: GCF_036786065.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Tn5403 promotes plasmid rearrangements and degradation of the
            Klebsiella pneumoniae carbapenemase (KPC) transposon Tn4401
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Sheppard,A.E., Barry,K.E., Parikh,H.I., Vegesana,K., Sebra,R.,
            George,S., Sanderson,N.D., Stoesser,N., Eyre,D.W., Crook,D.W.,
            Walker,A.S. and Mathers,A.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2024) Biological Sciences, University of Adelaide,
            Victoria Drive, Adelaide 5005, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 18x and 84x
            Sequencing Technology             :: Oxford Nanopore and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036786065.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:59:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,742
            CDSs (total)                      :: 5,620
            Genes (coding)                    :: 5,447
            CDSs (with protein)               :: 5,447
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 55 of 173
            Pseudo Genes (incomplete)         :: 129 of 173
            Pseudo Genes (internal stop)      :: 24 of 173
            Pseudo Genes (multiple problems)  :: 33 of 173
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP145160.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="CAVp363"
                     /host="Homo sapiens"
                     /db_xref="taxon:54291"
                     /plasmid="pCAVP363-8756"
                     /geo_loc_name="USA:Virginia"
                     /lat_lon="38.14 N 78.45 W"
                     /collection_date="2016-12-01"
                     /collected_by="Clinical Microbiology University of Virginia
                     Health System"
     gene            complement(join(8178..8756,1..399))
                     /locus_tag="LMH34_RS28600"
                     /old_locus_tag="LMH34_28600"
     CDS             complement(join(8178..8756,1..399))
                     /locus_tag="LMH34_RS28600"
                     /old_locus_tag="LMH34_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="WP_102016631.1"
                     /translation="MARNATNKLLQKAKKLKSDEFYTQYCDIENELQYYKSQFSGKSVY
                     CNCDDPRVSNFFKYFSVNFHELGLEKLIASCYVERDVDIFSNGVVKNGFYYEYRGESKE
                     GCVLDDIKPSYFDGDGDFRSSESIELLKKSDVVVTNPPFSLFREYLEQLIKYEKKFLII
                     ANVNSITYKEVFKLIKDNKLWLGVHLGRGVSGFIVPEHYELYGTEARIDASGNRIISPN
                     NCLWLTNLDTFIRHEDIPLTKKYFGNEAEYPKYDNFDAINVNKTKDIPSDYKGVMGVPI
                     TFLHKFNPDQFELIKFRKGMDGKDLSINGKCPYFRILIKNKRLQ"
                     /name="adenine-specific methyltransferase EcoRI family
                     protein"
     gene            complement(437..1270)
                     /locus_tag="LMH34_RS28605"
                     /old_locus_tag="LMH34_28605"
     CDS             complement(437..1270)
                     /locus_tag="LMH34_RS28605"
                     /old_locus_tag="LMH34_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196747.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000287 - magnesium ion binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcoRI family type II restriction endonuclease"
                     /protein_id="WP_102016632.1"
                     /translation="MSNKNQSNRLTDQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKDA
                     LSKEYPQLSFRYRDSIRKTEINESLKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDHDEW
                     RVVLVTEAKHQGKDIINIKNGVLVGKSSDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYILFLEGSNFLTENILITRPDGRVVNLEYKSGMLNRLDRLTAANYGMPINSNLCVNKF
                     VKHKDKNIMLQAASIYTQGNGEKWDSKIMFEIMFDISTTSLRVLGRDLFDQLTSK"
                     /name="EcoRI family type II restriction endonuclease"
     gene            complement(1706..1921)
                     /locus_tag="LMH34_RS28610"
                     /old_locus_tag="LMH34_28610"
     CDS             complement(1706..1921)
                     /locus_tag="LMH34_RS28610"
                     /old_locus_tag="LMH34_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1928..2425)
                     /locus_tag="LMH34_RS28615"
                     /old_locus_tag="LMH34_28615"
     CDS             complement(1928..2425)
                     /locus_tag="LMH34_RS28615"
                     /old_locus_tag="LMH34_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2466..2894)
                     /locus_tag="LMH34_RS28620"
                     /old_locus_tag="LMH34_28620"
                     /pseudo=""
     CDS             complement(<2466..2894)
                     /locus_tag="LMH34_RS28620"
                     /old_locus_tag="LMH34_28620"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3003..3608)
                     /locus_tag="LMH34_RS28625"
                     /old_locus_tag="LMH34_28625"
     CDS             complement(3003..3608)
                     /locus_tag="LMH34_RS28625"
                     /old_locus_tag="LMH34_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            3703..6600
                     /locus_tag="LMH34_RS28630"
                     /old_locus_tag="LMH34_28630"
     CDS             3703..6600
                     /locus_tag="LMH34_RS28630"
                     /old_locus_tag="LMH34_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(6597..6782)
                     /locus_tag="LMH34_RS28635"
                     /old_locus_tag="LMH34_28635"
     CDS             complement(6597..6782)
                     /locus_tag="LMH34_RS28635"
                     /old_locus_tag="LMH34_28635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338482669.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQREGASVLQGKPEEVRELID
                     ASPYVGSVPAEGEMTP"
                     /name="hypothetical protein"
     gene            complement(6772..7113)
                     /locus_tag="LMH34_RS28640"
                     /old_locus_tag="LMH34_28640"
     CDS             complement(6772..7113)
                     /locus_tag="LMH34_RS28640"
                     /old_locus_tag="LMH34_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7743..7892
                     /locus_tag="LMH34_RS28645"
                     /old_locus_tag="LMH34_28645"
     CDS             7743..7892
                     /locus_tag="LMH34_RS28645"
                     /old_locus_tag="LMH34_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_102016630.1"
                     /translation="MHEPPVQSDCCASSGNYRLESNPERHDKAPLAAAIGRQNVREMYR
                     EFLK"
                     /name="replication initiation protein"
     Typing          complement(7208..7381)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          7305..7902
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.917"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1591..2972)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.566"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6779..7092)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.3500000000000001e-58"
ORIGIN
        1 tttttttagc agttctatac tttcagaact gcgaaaatca ccatctccat caaaataaga
       61 tggtttaata tcatctaaaa cacacccctc cttactttcc ccacggtatt cataataaaa
      121 accgttctta accactccgt tgctaaaaat atccacatct ctttcaacat agcaagatgc
      181 tattaacttt tccaagccaa gctcatgaaa atttactgag aaatatttaa aaaaattact
      241 cactctagga tcgtcacaat tgcaataaac agatttccca gaaaattggg atttgtagta
      301 ttgaagttca ttttcaatat cacaatactg agtgtaaaat tcatcgcttt tcaacttttt
      361 agctttttgc agtaatttgt ttgttgcatt tctagccatt taaaaaatac cttacatatc
      421 aatactttat tacacatcac ttagatgtaa gctgatcaaa caagtctcga cccaacactc
      481 tgagcgaagt tgtagaaata tcaaacatta tttcaaacat aatttttgaa tcccattttt
      541 ctccattccc ttgagtatat atagacgctg cttgtagcat aatgttttta tctttatgtt
      601 ttacaaactt gttaacacaa agattactat ttataggcat cccgtaatta gctgcagtca
      661 gtcggtctaa cctatttaac atgccagact tatattcaag attaacaacc cttccatccg
      721 gtcttgttat taaaatattt tccgttaaaa agttagaacc ttctaaaaaa agtatatacg
      781 gaaagtgact ctcagaaagc ataaaatttg ctatttctga tatgttttta tgagaccttt
      841 cgatagcatt accagcagcc attaaatctt ggtcgctgga tttcccaacc aatacaccat
      901 tttttatatt tattatatct ttaccttgat gtttggcttc cgtaacaagc acaactctcc
      961 attcatcatg gtcgtcttta acctctacaa ttccaccatc aggcttgatg ctggaatttg
     1021 aaacaaaaag agtccctcct agatcagggt cgattttttt taaggattca tttatttccg
     1081 ttttccttat actatctcta tatcgaaaag ataattgagg atattctttg ctaagagcat
     1141 cctttactaa ttttgaaact tcgccaaccg cgagatcatg agcttttgca taatccccaa
     1201 aaatcccaat tacaccttga gataacttat gttgatcagt taatctattt gactggttct
     1261 tgttagacat gtatcaatct ttccactgtt attttatata tttacgtatt atcgataggt
     1321 taccatataa ttaatttacc ataacatctg ttacccgcac cgcgccggag gcactcaggg
     1381 cgtttctgat ggctgacggg caaaaggtgg tggcaagtac accgaaaacc accagaaacg
     1441 cacctgattt attttacgca tatgcatcac ttattcacat cactgcatag ccggtgattt
     1501 cggttaaaaa agactctctg tgtgctgaaa tcgcagaaaa tggccatgat ccccgtcacg
     1561 ctcaggccac ctgagacggg cgtttatcag tctccatact ccctgtcacc atgagataat
     1621 gtgcgttgcc gttccggtac cagatctgct gtcttctctg gttccttttc cgttctttgc
     1681 ccgtatcgct gttgctcaat ctcattcagc ctgtcgataa ccggcgcagc tgctcgctca
     1741 aactctgcac ctgcctgctc aagcccgaca cgcgctcgct cagcgccgcg ttctcccgtt
     1801 gcataagacc agacatcttc cgccattcct cgaaggcgct ctcccactcg tccagccttc
     1861 tcgagtagtc ctgctgtagc tgctctaacg cgctcagcaa ttgtttttcc agttccgtca
     1921 ttctgctcta ctcccgtacc agcttcaccg cgttccgcgt cttgttgtcc accgtccggc
     1981 ccgtttcggc tttcatcccc tccggcagca ccagaaaccg tccgttgcgg ttctccagat
     2041 aggtcacgcc ccacgtctgc atctgcagtt tcgacagcgt atctctctgc tgcctgattt
     2101 ccgccaggtt cgccgctatc cggttcccct ggtaccacag caccccgctc aggctggcga
     2161 acagcagccc tgacaccatg gtgatggtca gccacgtccg cccgaccatt cgcagcacgc
     2221 tcagccggtt cgactgcatc gccgcgttca tcccctgttc gtgggcgctg atggcgtcgt
     2281 tgattctctt cgcgcttgcg ttcagttccg atttgacaac gttctcgtgc ttgctgaatg
     2341 cggctttcag cctctcttcg gtaagctgct gctgtgcttt cgatttctcc tcgaagtccc
     2401 tcgaggccgt taaaagactg ctcataaatg gctcccctca gtcggatatt tcgccccccg
     2461 tccgggtcgg caatgctgat actgtttttt gtggttctga ccacctcaaa accggccgct
     2521 gtgagcgccc ctgtgacgtc atcacgcgtt ttcagctctc ctgatgaggc taacatcagc
     2581 aaaccccgtg taatcgtctc tgcggcttcc tgcttcgttt ctggcagtcc tgacggcgtg
     2641 accagcgccc gccggttctc cgggtcgttc ggatcatgta gccccagcct gccgttgacc
     2701 actgtctgcc acgcatcgat gcgcggccgg tcggcacgat cgtaatacgg ctggaggcgt
     2761 ttccccgtca gtaattcggt attggggatc agaaaattca gctcaagacg acccttgtct
     2821 gcgtgttcca cccacagaat gctgtactga tctttatcga gtccgggcat cagaacccgc
     2881 tcaaagctgg ccatcagttt ttcacgctga cccggcggca ggtccgcctc cgcgaatgac
     2941 agcacccccg aggtatattt tttcacatag gggtcggttc cggctgaggg cgaaatgaca
     3001 ccctaagcgt tagctctgtg tcgttgcacg atgtcagcga cggtattctt gctgataccg
     3061 agctcgcgtg cgatccagcg atagctgcgt ccctcggccc tcatcgcaac caccttaggc
     3121 aaaagtcggt ctgattttgg tcgcactccg gcctgacgac caagcctctt accacgtgcc
     3181 ttcgcaacag caaggcctga cttgacccgc tcgctgatga gatcccgctc aaactccgca
     3241 atgccggaaa gaaacgtcgc cagcattcgt ccatacggcg acgaaagatc gaacgccatt
     3301 ccattcatgg ctatcacgga aaccttccag ttctccagtt cacgtagcgt attgagcaga
     3361 tcgagcgtcg agcgccccca ccgggaaagc tcagtgacca ggattgcatc aatttgtctg
     3421 gactgggcaa gcgccaggac tttctttcgc tcggcccggt cgagtttagt tcctgaacct
     3481 gtttccttaa atattcccac cacgtcgtag ccggcacggc cggcgaaggc tcgcagatca
     3541 aattcctggc gttcacaaga ctgatccgct gttgaaaccc ggcagtaaat ggcggcacga
     3601 tgtcccaatt gaaccctcct ggatttttgt atcggaacgc cctgatttat atgggctggc
     3661 tgttgtccaa aacagactat acttcaaaag ggacgaattt gtatgtcacg acgccatatt
     3721 ttcaccgaac ggcagcgagc agcgctgttc gatctgccca cggacgaact gtcgctactg
     3781 aagttctaca cgctgggcga tgatgacctg gaaaacatta ggcagcgccg cagaccggaa
     3841 aacaggattg gctttgccct gcaactttgt gccttacgat atccgggccg tgcactggct
     3901 cctggtgaga tgatcccgcg tgaagtcctt tccttcgtcg gtgctcagct tggagttccg
     3961 gctgatgcgc ttctcactta tgccacacgg cgccaaaccc gtcagcagca catggacacg
     4021 ctgcgcgaaa tttacggcta caagaccttc acgggccgtg gtgcccgtga tctgcgggag
     4081 tggactttcg gccaggccga agatgccaga tcaaacgagg atcttgctca tcgttttatt
     4141 gtgcggtgtc gggaaacttc caccattctg cccgcagtat cgacaatcga gcgcttgtgc
     4201 gcggatgctc tggtcgccgc tgagcggcgg attgaaacgc ggattgcgga aaatttaaca
     4261 gcggatgttc gcgatcacct ggacaaactt ctgagtgaaa tgctcgccgg caatatcagt
     4321 cgtttcatct ggcttcgcaa cttcgaggtt ggtaacaact cggctgctgc taaccgtttg
     4381 ctcgacaggc tcgaatttct gcgtaccctg aatatcaatc atagtgcttt ggccagcata
     4441 cctgcccatc gcattgcccg gctgcgtcgg cagggtgaac gctacttcac cgacggtttg
     4501 cgtgacatca cttcggaccg ccgctgggcg atccttgccg tctgtgttgt ggagtgggaa
     4561 gcggcgattg ctgatgccat agtcgaaacc catgacagga tcgtaggaaa aacctggcgg
     4621 gaagcgaagc gccagcatga cgaaacaatt tccggctcta aagccacact cacggatacg
     4681 atccgtacct tcaccgcgct gggagcttcg ttgcttgagg cccgcagtga cggaaccccg
     4741 ctggagatgg ctgtcgccag ttcggttgca tgggaccggc tcgctcaact ggtagcgaca
     4801 gggactcaac tcagcaacac gctagccgat gagcctcttg catatgtcgg gcagggatac
     4861 catcgctttc gtcgttatgc gccccgcatg ttgcgctgtc tgaagctcga agccgcgccg
     4921 gtcgccggac cattggtagc agcagctttg tcgatcggag agatgaaagg tgttgcatcg
     4981 ccagaaaggc gtttcctgcg gcccagctcc aaatggaacc gtcatttacg agctcaggaa
     5041 aaaggagata cccgtctttg ggaagtggct gtactctttc acctccggga tgcttttcgt
     5101 tccggagatg tctggctcgc tcattcgcgc cgctatggtg acctcaagca ggtactggtg
     5161 ccgatgatcg cggcgcagga aaatgcaaaa ctggccgtgc cttccaaccc acaggattgg
     5221 ctggcagaca gaaaggcgcg actcacgatc gctcttaagc ggctggcccg ggctgcccgt
     5281 aacggcacta ttccgcacgg tagcatagaa gatggaacgt tgcggatcga caggttgaca
     5341 gcagacgtgc cggatggtgc cgaggcactc atactggatc tgtatcgccg aatgccgtcc
     5401 gttcggatta ccgacatgct gcttgaagtt gatgcagccc ttggtttcac agatgcgttt
     5461 acccatctga gaaccggggc tccatgtcgc gaccggatcg gtctgctcaa cgtcctgctc
     5521 gctgaagggc tcaatctggg cctgcgtaag atggcggaag ctacaaacac gcatgattac
     5581 tggcagctct cacgccttgc ccgctggcat gttgaaagcg aagccatgaa ccaggcattg
     5641 gcaattgtgg tggccgcgca gggtaaactg ccgatgtcac gcgtctgggg gatgggcacg
     5701 tcagcatcga gcgatggtca gtttttcccg acagcgcggc atggcgaagc catgaacatg
     5761 gtcaatgcca aatatggttc tgttcccggc ctcaaagcgt atactcacgt aagcgaccag
     5821 ttcgcgccat tcgcttgtca gtcgatcccg gcgaccgtga gcgaggcacc gtatattctc
     5881 gatggactac tgatgaacga ggtcggtcgc catgttcgcg aacagtatgc cgatacagca
     5941 ggattcaccg accatttgtt cggagccagt agcctgctcg gctacaatct cgttctgcga
     6001 atcagggatc tgccatcgaa gcggttgtac gtatttaatc ccgatacgac ccccagggag
     6061 ttacgcaagt tggtaggtgg aaaagcccgg gaggatctta tcgttgcgaa ctggcctgat
     6121 attttccgtt gtgccgcgac gatgaccgct ggcaaaatca ggcccagcca actcctgcgc
     6181 aagctcgctt cttacccacg acaaaacaac cttgcagttg cgcttcgtga agttggtcgt
     6241 attgaacgga cccttttcat tattgagtgg atcctggata cggacatgca gcggcgtgct
     6301 cagatcggtc ttaacaaggg agaggcccac catgcgctca aaaatgcgct ccgtatcggg
     6361 aggcaggggg aaattcgcga tcgcacgaca gaggggcagc actaccgaat cgctgggctc
     6421 aatttattga ctgcggtgat catttactgg aataccgtcc atcttggtca tgccgtcacg
     6481 gagcggcgga acgaagggtt ggatgttccc cctgaatttc ttccccacat atccccattg
     6541 ggctgggcgc acattctact gactggcgaa tatctttggc ccaaggaacc gaaagcttag
     6601 ggtgtcattt cgccctcagc cggaaccgac cccacatacg gcgacgcatc aatcagctcc
     6661 cggacttctt ccggcttccc ctgcaggacg ctggcacctt cacgctgccg gtctttaccc
     6721 agaagataat ccacaggacc ggcgcccccg ccgcgacccc ggggatggaa tttaacaatc
     6781 atcgtccggc cctttttcca gtacggcatg ccgcaaccgt tccagaccgg catcgatcgc
     6841 catcagcgct gccacgatct gcacccggtc atgaccggca ccaccggcgt tcactttccg
     6901 ggcgatctgg ttcaggttat tgcccatgcc agcaagctga cgcagcagcg cgggcgaaat
     6961 cgagggaagc ctgccggcgc gtgtcggttt ctcatcaagg caggtctggc gcatccacgc
     7021 cgccagttgt tttccgtcgc accgctcaag caaccgccgg tgttcgtctt ccgtcaccca
     7081 catcgtgagc atcttgttgc gtttatctgc cagaatcgcc tcctgataac gccgtgacgg
     7141 acgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcagtggctt
     7201 ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
     7261 gcttactatg ttggcacgga tgcggatatc agtgaagtgc ttcatgtgga aggaggaaaa
     7321 aggtggcacc ggcgcgtcag cggaatatgc gatacaggat atattccgct tcctcgctca
     7381 ctgactcgct acgctcggtc gttcggctgc ggcgagcggt gcgggcttac gaacggggcg
     7441 gaaatttcct ggtagatgct aggaaggaac ttaacaggga aacgataagg ccgcgccaaa
     7501 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     7561 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
     7621 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     7681 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     7741 gtatgcacga accccccgtt cagtccgact gctgcgcctc ttccggtaac tatcgacttg
     7801 agtccaaccc ggaaagacac gacaaagcgc cactggcagc agccattggt agacagaatg
     7861 tgcgagagat gtatcgagag ttcttgaagt agtggcctaa ctgaggctac actggaagga
     7921 cgaatttggt tacatgtctc gcacaagact gttaccacgg ttagagaatc tgccaggatt
     7981 atctaacctt cgaaaaacca cctgccaggg tggttttttc gtttctggtg ctgaagatta
     8041 cgacgaccag ctaatgatct caagaaaaac ctaacaaaac cctacacatc acgttgttac
     8101 aatgagacca aaggcagctt ttaataaata attcggcttt tactataaag ctctaccgca
     8161 taagcccaca ttaatcacta ctgtaacctt ttgtttttta ttagaatcct gaagtatggg
     8221 cacttcccat taatagataa atctttgccg tccattccct ttctaaattt tatcaattca
     8281 aactgatcag gattaaattt atgtaggaat gttataggca ctcccataac ccccttgtaa
     8341 tctgatggga tgtcttttgt tttatttaca tttatagcat caaaattatc atatttagga
     8401 tactcagctt cattaccaaa atattttttg gtaagaggta tatcttcatg acggataaaa
     8461 gtatccaagt ttgtcagcca tagacagtta tttggtgaaa taattctatt tccactcgca
     8521 tcaatccttg cctcggtccc atataattca taatgttcag gaactataaa tccggaaacc
     8581 cccctcccga gatgaactcc aagccaaagt ttattgtcct taatgagttt aaacacttct
     8641 ttatatgtta ttgaattaac attggcaatt ataagaaact tcttctcata cttaattaat
     8701 tgttcaagat actctctaaa caacgagaac ggcggattcg taacaacaac atctga
//
