LOCUS       NZ_CP144709             4510 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain B18235 plasmid pB18235_7, complete
            sequence.
ACCESSION   NZ_CP144709
VERSION     NZ_CP144709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39434441
            Assembly: GCF_036687385.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: B18235
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036687385.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:43:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,646
            CDSs (total)                      :: 5,522
            Genes (coding)                    :: 5,405
            CDSs (with protein)               :: 5,405
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 47 of 117
            Pseudo Genes (incomplete)         :: 72 of 117
            Pseudo Genes (internal stop)      :: 15 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18235"
                     /isolation_source="River estuary"
                     /db_xref="taxon:573"
                     /plasmid="pB18235_7"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            412..858
                     /locus_tag="VXE08_RS28205"
                     /old_locus_tag="VXE08_28205"
     CDS             412..858
                     /locus_tag="VXE08_RS28205"
                     /old_locus_tag="VXE08_28205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125450400.1"
                     /translation="MNDKSGFSFMKIGAGAKVTGLRMKRNVMVVDDLSHVESFVTVGER
                     GEVINLDADGNELHTPDSYREKLKIERGELQASLEKAKEEINSIDNDITSQKIKNQLSA
                     ALSQPMDAEGEWNTKRILQGVLDLSKQVGCGVLTAAITHYLGVS"
                     /name="hypothetical protein"
     gene            complement(1400..1642)
                     /locus_tag="VXE08_RS28210"
                     /old_locus_tag="VXE08_28210"
     CDS             complement(1400..1642)
                     /locus_tag="VXE08_RS28210"
                     /old_locus_tag="VXE08_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_071993219.1"
                     /translation="MALVRSGNKTKIFEADPKFNPVFDTQHKPGDTCTYEGIYKCTGCG
                     KEVTIAGGKTLPPQNHHQHSPGQGDIRWQLFVYAE"
                     /name="protein L"
     gene            1825..2145
                     /locus_tag="VXE08_RS28215"
                     /old_locus_tag="VXE08_28215"
     CDS             1825..2145
                     /locus_tag="VXE08_RS28215"
                     /old_locus_tag="VXE08_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_072198282.1"
                     /translation="MQQNHDSDDDCRIQFRDDWLYQFYKYGKANRKIPATISVSLGRKL
                     DMLNAADKIGDLRSPPGNRFENLNPPLTGYSSIRVNDKYRLIFKWGENGVEDLYLDPHD
                     YR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2349..2705
                     /locus_tag="VXE08_RS28220"
                     /old_locus_tag="VXE08_28220"
     CDS             2349..2705
                     /locus_tag="VXE08_RS28220"
                     /old_locus_tag="VXE08_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_032413514.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIERVTPAHEIIER
                     KHNAESLEKKHAVA"
                     /name="HigA family addiction module antitoxin"
     gene            3534..3725
                     /locus_tag="VXE08_RS28225"
                     /old_locus_tag="VXE08_28225"
     CDS             3534..3725
                     /locus_tag="VXE08_RS28225"
                     /old_locus_tag="VXE08_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3725..4135
                     /locus_tag="VXE08_RS28230"
                     /old_locus_tag="VXE08_28230"
     CDS             3725..4135
                     /locus_tag="VXE08_RS28230"
                     /old_locus_tag="VXE08_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060578860.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKIIKVID
                     TSELIRVFGSLDGVIDTVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKARDEH
                     IDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3006..3415)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgtggtga gtcgtccggc gacttgcata acgtgattgc cggccgtcga cgaacccgat
       61 tcctgaacgt gctgaaaacc gtttttccgg ctgcgccggc aacgcttcac ggccacgcaa
      121 taaattgcgt ccccgttcac cgttttcagt ctggtttggt gcgggttttg agttgtctgg
      181 cctgaattca ggctgaaatc cgccaccagt cagtctggtg cataacaatt catgcccgta
      241 aaacgccctg gagcgatttt gaggcgtttt cagggggtaa gacgtaggat ttaatggatt
      301 acgccccaga atcgtttcta gagcgtttga gcggtgcgcg taatgaggcg ttatggtaaa
      361 taattgctcg tgaaaacgtt attaacattt ttatcttatg aggtgatgtg agtgaacgat
      421 aaatcaggct ttagttttat gaagatcggt gcaggtgcaa aagtgactgg tttaagaatg
      481 aaaaggaatg tgatggttgt tgatgatctg tctcacgtag agagctttgt tactgttggt
      541 gagcgaggcg aagttattaa tctcgatgcg gatgggaacg aactacacac accagacagt
      601 tatcgtgaaa agttaaaaat agaacgcggt gaactccagg cttccttgga gaaagccaag
      661 gaggaaatca actcaataga taacgatata acttcgcaaa agattaagaa ccagttgagt
      721 gctgcactat ctcaaccaat ggatgcagaa ggtgagtgga acactaagag aatcctacaa
      781 ggggttttag atttatctaa acaagtgggc tgtggtgtac tcacggctgc gatcacgcat
      841 tatcttggag ttagttaata aactacctat gcccccatgg tgggtataca aaaaccatgg
      901 ggagcggagc gaccttcgtc tagctctttt cttgtgtgtc tcatcttgca acggattcga
      961 tttgtgtggg gttagtgtca gacttcttgg ggtcagtttg gatatagaga attattgtac
     1021 ggtaagcctg tgttttgaac aggcttacct ctcgcaatga tgtactcccg cgctatacgc
     1081 cgtttcccgt gaattttcct taatcggata acctaaatgc gatacagaaa gagcggtgac
     1141 ttaaaagcga tcgtagctga ggtaaaatca atcccttacg catcaatatt ggttaaataa
     1201 tgcgcttaac gtacaaaagt ttccgatatc caaactgacc cctatttggc ggcttttctt
     1261 attttcttga ttttcccgca tctgaacgtt tctctcggac tcttccatta ttattacgat
     1321 ctcgtggctg agttccgcct cctggtttag ggacgacgac atgcgggtta agtggtctac
     1381 ggtctgccat tttcatacct cactctgcat aaacaaatag ttgccagcgg atgtccccct
     1441 gtccgggact atgttgatga tgattttgtg gtggaagtgt tttccctcct gcgatagtta
     1501 cttctttacc gcaacctgtg catttgtaaa ttccttcata cgtgcacgta tcgccaggct
     1561 tgtgttgtgt atcaaaaact gggttaaatt tcggatctgc ttcaaaaatc ttggttttat
     1621 ttcctgagcg aacaagcgcc atatgacctc caactattct ttttttgcac agtagcactg
     1681 tgcttttgtg cattttatat cattatggtt ttttgcacaa gtgcacagtg ctattgtgca
     1741 tttctggcgt ttgttgtact atggtggtgg taaaaggtga tctagaactt gccaagttgc
     1801 ggcaacattt gaggtaaaag aaaaatgcag caaaatcatg atagtgatga tgattgccgc
     1861 attcagttcc gagacgattg gctataccag ttttataaat acggtaaagc caatcgaaaa
     1921 attccagcta cgattagtgt ttcattaggt cgcaagctgg atatgctgaa tgctgctgat
     1981 aaaattggag atttacggtc tcccccgggg aaccgatttg aaaatctcaa ccctccattg
     2041 acagggtatt catctataag ggtgaatgat aagtaccgct tgatatttaa gtggggtgag
     2101 aacggagtcg aggatttgta tctcgatcca catgattacc gataggtaac agaccggaac
     2161 tgttatctta caaaacaagt aggtgaggca tttcctcacc tacgttaaaa cgtcatcaca
     2221 aataatgatt aataaagtaa cgcgacgtaa ctatatttaa gataattact aatcgataca
     2281 gctggtaaca gaagaaatac ttatcttcct catctaacag agagaagcga ccaattcagg
     2341 agtaaagcat gtcccaagca accagaaaac catcgactgt aggagatatt attctttatg
     2401 aatatctcga gccaagtggt ttaaaaatca atgacttggc tgaaatgtta aacgtgcata
     2461 gaaattctgc aagtgctttg gttaacaata ataggaaact gtctaccgat atggcgtttc
     2521 ggttagctaa agcattcgat actaccgcta agttctggtt aaatatacag cagtctgttg
     2581 atttgtggga ggttacgagc agcccacgcc ttcaggaaga aatagaaaga gtcactcccg
     2641 ctcatgaaat tatagaaagg aagcacaatg ctgaatcttt ggaaaaaaaa catgcggtcg
     2701 catgattttt ttaaaaaaat ttaaccaaac tagaagaagg cctctgtaat agtgtgcctt
     2761 ctttttttgt aaccgctaaa ggtcatcatc attttctttt acgagcactt gagatccggt
     2821 ttcggtacgc gtaatctctt gctctgtaaa cggaaaaacc gccttgcagg gcggtttttc
     2881 cgaaggttca aacacttgca ggtgattgaa ccaaggtaac tggcttggag gagcgcagtc
     2941 accaaaactt gttctttcag tttagcctta acaggcgcat gacttcaaga ctaactcctc
     3001 taaatcagtt accagtggct gctgccagtg gcgcttttgc atgtcttacc gggttggact
     3061 caagacgata gttaccggat aagacgcagc agtcggactg aacggggggt tcgtgcatac
     3121 agtccagctt ggagcgaact gccttcccgg aactgagtgt ctggcgtgga atgagataaa
     3181 cgcggccata acagcggaat gacaccggta aaccgaatgg caggaacagg agagcgcacg
     3241 agggagccat cagggggaaa cgcctgatat ctttatagtc ctgtcgggtt tcgccaccac
     3301 tgatttgagc gtcaatttct atgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     3361 cgctaagcgg cctttccttc tgcccatcac gcgtccgctc ggttttctcg agcatttata
     3421 cgcatagtcc taccgctcgc cgcagccgaa cggcaggagc gtagcgactg agtgagcgag
     3481 gaagcggaaa agagactggt tttacactga gcactgacgc tctgaggctt cttatgacga
     3541 aacaacagaa aacagcgctg aacatggcta aattcatcca gaatcagtct ctgctcctgc
     3601 tggagaagct taacgagctt gatcttgatg cagaggccga cctttgtgag aaactccacg
     3661 atgatgcgga gcatcttttc cgtacactgt cctcgagact ggatgaatta caggatggta
     3721 actgatggcc agagtgtcta ttagtgaagc ggcacggctt gttaaggtta gtcgccccac
     3781 aatttacaag atgattaaca gtgggaagtt aagttacacc tctgttgtaa agcacgggaa
     3841 gatcattaaa gttattgata cttcagagct tattagagtt tttggttctc ttgatggtgt
     3901 catcgatact gtaaaaagtg acgcaggatc tacaggtgtt aacagcgttg gtttacatga
     3961 tttacaacat agaattgctt tactagaagc tgaaaacgat ggtcttaaag gggctgttaa
     4021 ggctcgagat gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaagca
     4081 cgaaccttct tcaccacccg attccccgtg gtggaaattc tggaaaaaat cctgactccg
     4141 tcatgcttcc gttttcctct ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg
     4201 ccagatctct gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaaaatt
     4261 ttgaagataa ttcattaata tcagttagtt gtgggttatt acattacgca gtattacttt
     4321 ctgtaaggtg aatccaagtc gtcggcgatt cgtgtacgac ttgccgacga ctcgtcatag
     4381 agtcatcgac gactcactta agactatcag taacttatga cgacttgact ctagagtcga
     4441 atcgtcattt tggtgttttt ttgaccttgg cgactctaga gtcaagtcac gagtcgaatc
     4501 gccggtgaat
//
