LOCUS       NZ_CP144708             4865 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain B18235 plasmid pB18235_6, complete
            sequence.
ACCESSION   NZ_CP144708
VERSION     NZ_CP144708.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39434441
            Assembly: GCF_036687385.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: B18235
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036687385.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:43:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,646
            CDSs (total)                      :: 5,522
            Genes (coding)                    :: 5,405
            CDSs (with protein)               :: 5,405
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 47 of 117
            Pseudo Genes (incomplete)         :: 72 of 117
            Pseudo Genes (internal stop)      :: 15 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144708.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18235"
                     /isolation_source="River estuary"
                     /db_xref="taxon:573"
                     /plasmid="pB18235_6"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            729..953
                     /locus_tag="VXE08_RS28170"
                     /old_locus_tag="VXE08_28170"
     CDS             729..953
                     /locus_tag="VXE08_RS28170"
                     /old_locus_tag="VXE08_28170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            955..1599
                     /locus_tag="VXE08_RS28175"
                     /old_locus_tag="VXE08_28175"
     CDS             955..1599
                     /locus_tag="VXE08_RS28175"
                     /old_locus_tag="VXE08_28175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            1599..2075
                     /locus_tag="VXE08_RS28180"
                     /old_locus_tag="VXE08_28180"
     CDS             1599..2075
                     /locus_tag="VXE08_RS28180"
                     /old_locus_tag="VXE08_28180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(2282..2395)
                     /locus_tag="VXE08_RS28185"
                     /old_locus_tag="VXE08_28185"
     CDS             complement(2282..2395)
                     /locus_tag="VXE08_RS28185"
                     /old_locus_tag="VXE08_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            2811..3002
                     /locus_tag="VXE08_RS28190"
                     /old_locus_tag="VXE08_28190"
     CDS             2811..3002
                     /locus_tag="VXE08_RS28190"
                     /old_locus_tag="VXE08_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3002..3424
                     /locus_tag="VXE08_RS28195"
                     /old_locus_tag="VXE08_28195"
     CDS             3002..3424
                     /locus_tag="VXE08_RS28195"
                     /old_locus_tag="VXE08_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3607..4799)
                     /locus_tag="VXE08_RS28200"
                     /old_locus_tag="VXE08_28200"
     CDS             complement(join(3607..4523,4523..4799))
                     /locus_tag="VXE08_RS28200"
                     /old_locus_tag="VXE08_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612052.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISKpn18 family transposase"
                     /protein_id="WP_085955245.1"
                     /translation="MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLTE
                     SVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELFKKSFGLLRTGN
                     KVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGR
                     TYGSRRLSAALRAQGHDVGRHRCRTLMKACGLKACWRRKFAHTTDSKHHLPVAENVLNR
                     QFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMA
                     ISVRQPAPGLLVHSDRGSQYASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKM
                     ERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAAQLPACA"
                     /name="IS3-like element ISKpn18 family transposase"
     Typing          complement(2283..2690)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 gtattttata tcagtagttt acgttctata aatttcgttt ttttaatttt atttatacag
       61 atccgactct atgacgactc gtcatagagt ccccggcgag tcgtcataga gtcggtaacg
      121 actcaggact ataaaaacaa taagttaaca acgacccgac tctagacccg actccccgtt
      181 tggttgtttt ttagacgtga cgactctggg tacgactcta gtgaagagtc gccggtgaat
      241 cgtgtggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacccga
      301 ttcctgaacg tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca
      361 ataaattgcg tccccgttca ccgttttcgg atgggaagaa cgccggaaac aggacttacc
      421 ctggctgaat ggcgtgaatt ttcgcgctat gcacttgcgt gcatactcat gcatgccgta
      481 aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgccta ttttaccggt
      541 ttcccgtcag aaacgccctg aggccgtttt agcggtgcgt acaattttgg tgttatgtta
      601 aatcatggcc acaaaattcc ataaagtgac caatgtaatg ttaaatgagc attgtggatg
      661 aaagatgttg ttttatggta ttatttttta tgttctaaac ttaaatttat ttctatcggg
      721 gtattataat gagcaataaa gattctttag aaagaaaagt aacaccaaga aaaaatgaac
      781 ctgtattccc gatggctcta agagttggga tagctgaaga tatttgtttt ttagagttag
      841 tggactttcc tgaaaaagaa tcaggaaggg ttttttattc catagctcta accaaaaaac
      901 atgcaaagaa ccttatcgat ggattgaata aattcgttga gtcaggtgat tagtatgaaa
      961 tacattaacc actgcgatag tgaagattat tatattacta atgatagcat tgatctcggt
     1021 gttcgttatg atgtttctga ttataataag ttagagctaa atgatttaag agatccggat
     1081 ttgtttaaag ttttgggtcg agcatttgtt gttgttgaga attctgtttg ggagtcttgg
     1141 aatgatgatg ttaaaacatt agattgctct ggtgacggtt atgagtcaat tttaaaacat
     1201 tacgaatcat tagaattatc aggtgaattc ttattgtttt ttaaaaaaat aagaagtgcc
     1261 atttctgttt ataatagaga atacgagttc tgcactggtg acctagaacc atctttgcag
     1321 gctattgttc agttgattag gtatgcccct gaacttgctg caaaaaaaga cgctcagatt
     1381 tactttgata aaaaatcaga gtgttttggg cttatatata aacaaactaa gaaaagcaaa
     1441 ggcacgctaa atttaatagt gaaaacaaat aacgagatta ttttttcctt tgttagaaaa
     1501 gtttctggag tggttaaggt tacaggtagg gcatatattg gcgatagcct tgataactcc
     1561 attgctatca aatcattatt taaaatgatg gagtggtgat gactgtgtta tcaactttaa
     1621 ataacctcaa atcaatccct tgtattaatg catttacggg cagagcaaat aattcatatt
     1681 tatggcgtta tgttatggaa gatcataaag atgagtttgg cgctacaact attaagtgtt
     1741 tttatcttag ggaggaaatt tcagaggatt atgtgtctat ttatcattca aataaagatt
     1801 attatgaagg aatggtaagg gatgtacata aaagaatccc tgtagggaaa accaaacctg
     1861 ccatatatct ctcatttgat gcagcggaag tgatcgagca agttaacaaa ccagataaat
     1921 gtattgatat aaaggacgaa ggttaccctc acttgggttt gtggtattta tttgatatta
     1981 atgatgaatt tactttgatt gaggtgacta caactatttt ggaattatcc tctttttttc
     2041 aaagaaatga aggtggcgtg gttgttcctc tttagcgaaa accgtcttga cgtcatcaag
     2101 tgtgacctag tactcttctt gaaaaagaaa aaccgccttt gcggggcggt ttttcttagg
     2161 ttctaagagt ttgcggctct atgaaccaag gtaactggct agttagagga gcgcagtaac
     2221 caaaatcgtt ctttcagttt agccttaact ggcacataac ttcaagacta actcctctaa
     2281 atcagttacc agtggctgct gccagtggcg cttcgtcgtg tctttccggg ttggactcaa
     2341 gttgatagtt accggaaaag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     2401 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc
     2461 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2521 gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2581 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggccgc
     2641 tgtgcggcct ctccttctcc ccatcacgcg atcgctcggt tttctcgagc ttttatacgc
     2701 atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt gagcgaggaa
     2761 gcggaaaaga gaacatccct acactgagca ctgacgctct gaggcctctt atgacgaaac
     2821 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg
     2881 agaagcttaa cgagcttgat cttgatgtag aggccgacct ttgtgagaaa ctccacgatg
     2941 atgcggagca tcttttccgt acactgtctt cgggactgga tgaattacag gatggaaact
     3001 gatggccaga gtgtctatta gtgaagcggc tcggcttgtt aaggttagtc gccccacaat
     3061 ttacaagatg attaacagtg ggaaactaag ttacacctct gttgtaaagc atgggaaaaa
     3121 tactaaagtt gttgatacat cagagcttat tagggttttt ggctcgcttt atggtggggg
     3181 cgacactgta aaacatgatg taaaaggtga cgcaggcgct acaggggtta acatctctgg
     3241 tttacatgat ttacagcata aaattgcttt gcttgaagct gaaaacgatg gtcttaaagg
     3301 ggctgttaaa gctcgtgatg aacatatcga ttctcttcgc caggctatgc agcttctgga
     3361 acacaagctc gaaccctctc cacaggttca ttctccgtgg tggaaattct ggaagaaatc
     3421 ctgaccccgt catgcttcag ttttcctctc cggccattcc ggggagttcc tgaaccttcc
     3481 caaaaatcgc cagatctctg aacgctcctc accactaaaa atatccacac cccacgcaaa
     3541 attaaaattt tgcagatttg tatgttgtgg tcaagttttt ttggacacgc cgatcagatg
     3601 gtagggttat gcacaggcgg gcaactgggc ggcagggctg gccagcgttt cgtactgtgc
     3661 gggtgatcgg tagccaagtt ttgaatgcaa tcgggtactg ttataaaagt taacgatgta
     3721 gtcggtgatg tcttgtttgg cttctgtatg gttggcatac cgagtctgcc agacacgctc
     3781 cattttcaaa ttcagaaaga accgctccat cacggcattg tcccagcaat tacccttgcg
     3841 gctcatgctg agtaccagac catggttttc aagtaattgc cggtgttcag cactggcata
     3901 ttgactgccc cggtccgaat gaaccaataa acccggtgcc ggttggcgaa ccgaaatagc
     3961 catgctcaac gcatcgcaca ccagctgtgc aggcatggtc ggtgccatgg accaacccac
     4021 gatgcgacgt gaatacagat ccagcacagc ggccagatat agccaaccgc tgtcggtccg
     4081 aatgtacgtc acatcgcaca cccaggccgt attgggtgct tcaggctcaa actgacggtt
     4141 gagcacgttc tctgccaccg gcaaatggtg tttgctatcg gtggtatgag cgaacttgcg
     4201 acgccaacag gccttgagtc cacaggcttt catcaaggta cgacagcgat gacgtccaac
     4261 atcatggccc tgggcacgca gggctgcact caaacgacga ctgccatagg tgcgaccact
     4321 ggcaagaaaa accgcctgag catgcacgct gtcctggcaa ataggtttgg gtgtattgac
     4381 tctgcgacgc gctgcgtagt aaccagagcg actgatgtcc aacgcctgac agacctgctt
     4441 gatcggggcc tgcttcgcta actcagtgac aacatcgtgg atcactttat ttcccgtgcg
     4501 aagaaggccg aagctttttt taataattca ttgtctgatt ttagtcgcct gacttcggct
     4561 tccagttctc gaatacgttg ctgctcagcc gttaacggtt taccaatccc cggcctgccg
     4621 gctgactcgt catccagttg gtgtacccag cgtcggacca cactttcggt cagcttcatt
     4681 tcgcggcaga cttggctgac gctgacgcct tgctcgcgca ccatgcgcgc cacctgtaac
     4741 ttgaacgctg gatcaaaggt tcggcgattc ttggttgtgt cggttgtctt gtcggtcatc
     4801 tcttcatgtt ccttgtcggg gggaaaacac ccgatcaaag tgtccattta aatttgacca
     4861 caaca
//
