LOCUS       NZ_CP144706             5840 bp    DNA     circular CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain B18235 plasmid pB18235_4, complete
            sequence.
ACCESSION   NZ_CP144706
VERSION     NZ_CP144706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39434441
            Assembly: GCF_036687385.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: B18235
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036687385.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:43:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,646
            CDSs (total)                      :: 5,522
            Genes (coding)                    :: 5,405
            CDSs (with protein)               :: 5,405
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 47 of 117
            Pseudo Genes (incomplete)         :: 72 of 117
            Pseudo Genes (internal stop)      :: 15 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18235"
                     /isolation_source="River estuary"
                     /db_xref="taxon:573"
                     /plasmid="pB18235_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            317..508
                     /locus_tag="VXE08_RS28095"
                     /old_locus_tag="VXE08_28095"
     CDS             317..508
                     /locus_tag="VXE08_RS28095"
                     /old_locus_tag="VXE08_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            508..909
                     /locus_tag="VXE08_RS28100"
                     /old_locus_tag="VXE08_28100"
     CDS             508..909
                     /locus_tag="VXE08_RS28100"
                     /old_locus_tag="VXE08_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714819.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADHSEQVISHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1658..2203)
                     /locus_tag="VXE08_RS28105"
                     /old_locus_tag="VXE08_28105"
     CDS             complement(1658..2203)
                     /locus_tag="VXE08_RS28105"
                     /old_locus_tag="VXE08_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048981308.1"
                     /translation="MPLPERMKPARVTRKQMKELISQLNGILDHIDNGMDENNEELKQM
                     MADWNAKVVMPCGFSDFRDFSSWTSALDFTRMALNQEKYLDDFTWTELNDVINFIRKAE
                     GSESDHSYALQLLEINFKANPLDLIYHPDCWFNDEALFHARLTTEEIGGYLMKKSGRWL
                     NDAPDIQLVYAIPLDVYD"
                     /name="hypothetical protein"
     gene            2578..2970
                     /locus_tag="VXE08_RS28110"
                     /old_locus_tag="VXE08_28110"
     CDS             2578..2970
                     /locus_tag="VXE08_RS28110"
                     /old_locus_tag="VXE08_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048981310.1"
                     /translation="MDLIITVRVLRAQALALLLPVMWLISLIVLVGRKSGHQMSRIFAV
                     MVMVGIWRIFGGRFTLCPLKKAQQLQQLSVASMVSAVLLPVMAEVRVGRGHRDSLSLCI
                     TGRWDTGKTDHPGLVITNILPALLWQ"
                     /name="hypothetical protein"
     gene            2883..4901
                     /locus_tag="VXE08_RS28115"
                     /old_locus_tag="VXE08_28115"
     CDS             2883..4901
                     /locus_tag="VXE08_RS28115"
                     /old_locus_tag="VXE08_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_309491503.1"
                     /translation="MHNGQMGYWENRSSGAGNNEHTTRAFVAVGPSEAEKAARAEKVAQ
                     EKQQAEEAAKAFAAKTAAASAAAEKERQNAISAAAAAGQHQTVPDARNNLNQATAEASR
                     LKTVADNALNTAKNKRKEAIDAVPVATQAEKKYQDLQQSIKGLTLNNNGQYGTQKWEVI
                     SSNKEHDHWGYRFYPSGITKAQVDAAQNDAVNKRNAATSLASQATAAEQASLQASAAYN
                     AAETRRQAAQAALASAEQAAAAERKRHEAEAAAAAAAEKKRQADAAAKAAEEARAIAEK
                     AKALQARRTAADKLKSSEIQAVRGIPATAAPFAIPLTWSTASRGSFTLAADAAASLGAF
                     ISEALATLRVAVVANPVALTIAGLVLSKSVGVGSDMVPGRDISSMMPGDAFGLPDTAAL
                     NKAADQKTSVSMPVRGRLVMNDSGILDVQLVKTNTAGAVKVARAVLDKETGYWGYTLPA
                     VADVPAQTIFVSPADAPGANGPLTLSGPVPLPERILHTGDQISAPQATDKTVTPVADDL
                     DFDDIILVFPPESGLKPLYVMYRSPRNMPGTVSGKGQSVGNNWMGGASTGDGAPVPSQI
                     ADKLRGKTFGSFDSFRRAFWKAVADDSALSKQFSEADINQMKAGRAPTADFLESVGKRV
                     KIELHHEKEISQGGAVMDVDNIKALTPKNHIETHKGK"
                     /name="S-type pyocin domain-containing protein"
     gene            4903..5160
                     /locus_tag="VXE08_RS28120"
                     /old_locus_tag="VXE08_28120"
     CDS             4903..5160
                     /locus_tag="VXE08_RS28120"
                     /old_locus_tag="VXE08_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_032413518.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKIGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            5268..5423
                     /locus_tag="VXE08_RS28125"
                     /old_locus_tag="VXE08_28125"
     CDS             5268..5423
                     /locus_tag="VXE08_RS28125"
                     /old_locus_tag="VXE08_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(5628..5741)
                     /locus_tag="VXE08_RS28130"
                     /old_locus_tag="VXE08_28130"
     CDS             complement(5628..5741)
                     /locus_tag="VXE08_RS28130"
                     /old_locus_tag="VXE08_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049026061.1"
                     /translation="MHEPPVQSDCCALSGNCHLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(2..196)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.919"
                     /coverage="99.0"
                     /evalue="6.82e-71"
     Typing          complement(5629..5840)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.714"
                     /coverage="92.0"
                     /evalue="3.04e-95"
ORIGIN
        1 tggcaggaac aggagagcgc acgagggagc catcaggggg aaacgcctgg tatctttata
       61 gtcctgtcgg gtttcgccac cactgatttg agcgtcaaat tctgtgatgc ttgtcagggg
      121 ggcggagcct atggaaaaac ggccgctggg cggcctcttc ttttccgcct cccttgatcg
      181 ctcggttttc tcgagctttt ataagaacgg tcttgccgct cgccgcagcc gaacgaccgg
      241 agcgtagcga ctgagtgagc gaggaagcgg aaaagagatt gttcttacac tgagcactga
      301 cgctctgagg cttcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat
      361 ccagaatcag tctctgctcc tgctggagaa gcttaacgag cttgatctcg atgtagaggc
      421 cgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag
      481 actggatgca ttacaggatg gtaactgatg gccagagtgt ctattagtga ggctgcccga
      541 ctgacgggta agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc
      601 tgtagcggtg aacgcaatgc aaaaatggta gatacttcag agcttttacg tgtctttggt
      661 ccctttgaac aaccgaaggg tgaacaccat tctggacagg tctctgaaca gcgtgataca
      721 gcctctgctg accatagtga acaggtgatt agtcatctga aacaggagat tgaacacctg
      781 aacaccttac ttttgtccaa ggattcgcac attgacagcc tgaaacaggc catgctcctt
      841 cttgaaaatc accaggcaaa attaactgaa tccccggcgc catggtggaa attctggaaa
      901 aaatcctgac cccgtcatgc ttccgttttc ctctccggcc atcccgggga gttcctgaac
      961 cttcccaaaa atcgccagat ctctgaacgc tcctcacgac taaaaatacc cacacactac
     1021 gcaaaattaa aattttgcag ataactcctt aatatcagtt agttagatgt tattaaatta
     1081 cgctgtatta cattctgtaa ggtaaatcca agtcgtcggc gattcgcgta cgacttgtcg
     1141 atgactcgtc atagagtcat cgacgactca cttaagacta tcagtaactt atgacgactt
     1201 gactctagag tcgagtcgtc attttggttt ttttttgacc ttggcgactc tagagtcaag
     1261 tcacgagtcg aatcgccggt gaatcgtgtg gtgagtcgtc cggtgacttg ccataacgtg
     1321 attgccggcc gtcgacgaac ccgattcctg aacgtgatga aaaccggttt tccggctgcg
     1381 ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatagga
     1441 agaacgccgg aaacaggact taccctggct gaatggtgtg aattttcgcg ctatgcactt
     1501 gcgtgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg
     1561 tggtttgttg cctgttttgc cggtttcccg tcggaaacgc cctgaggccg ttttagcggt
     1621 gcgtacaatt aagggattat ggtaaatggt gacttggtta atcataaaca tccaggggga
     1681 tggcatacac cagttggata tccggtgcat cgttgagcca tcgacctgat tttttcatta
     1741 agtagccacc aatttcttcg gtagtcagcc gggcatgaaa caatgcttca tcattgaacc
     1801 agcagtcagg gtgatagatg agatccagtg ggtttgcctt gaaattaatc tccagtaact
     1861 ggagtgcata gctgtggtca gattcactac cttctgcttt gcgtatgaag ttgataacgt
     1921 cgttcagttc tgtccatgta aagtcatcga ggtatttttc ctgattaagc gccatcctgg
     1981 tgaaatctag tgcacttgtc caggatgaaa aatcacgaaa atcagagaac ccgcagggca
     2041 tgacgacctt tgcattccag tctgccatca tctgttttag ttcttcattg ttttcgtcca
     2101 tcccgttatc gatgtgatcg agaatgccat tcagctgact gatcagttct ttcatctgtt
     2161 tacgggtgac ccgtgcgggt ttcattcgct caggtaatgg catcagaaat actctctttt
     2221 ttatcgtgca ctcgggggaa ctgagtaatc tatcgggcta tgaatcagag cacaaatcgt
     2281 ttttttttct ttctgatgat ccgcattctg cctcagattt acctctgtcg taacttttaa
     2341 ccgattgaaa ataaataaaa atattgttga cgtctgcata ttgacagagc tggcatgtca
     2401 agagtagtat caaactactg tatataaaca catgttttta tatacagtag tttggcgtgg
     2461 cagagaacta ataacagcct ccggataact gtcctgaagc aataaaccgc acgccggatt
     2521 tcagaaaagt ggaaaacaaa aaaaacaaaa aaaacaaaag aaggaaatgt gtgatgggtg
     2581 gatttaatta taacggtgag ggtgctacga gctcaggcct tagctctcct tctgccagtg
     2641 atgtggctaa tcagtttaat agttttggtg ggacgcaaat ccgggcatca gatgtctcga
     2701 atattcgcag tgatggtaat ggtgggtata tggcggatat tcgggggcag attcacactg
     2761 tgtcctctga agaaggctca acaacttcaa caactgtcgg tggcttcgat ggtgtccgca
     2821 gttcttctac cggtaatggc ggaagtcagg gtgggcaggg gccacaggga cagtctctcg
     2881 ttatgcataa cgggcagatg ggatactggg aaaaccgatc atccggggct ggtaataacg
     2941 aacatactac ccgcgctttt gtggcagtag gcccatcaga agcagaaaaa gctgcacgag
     3001 cggagaaggt agctcaggaa aaacagcaag cagaagaggc tgcaaaagca ttcgcagcga
     3061 aaacagcagc ggcatccgcc gctgctgaaa aagaacgtca gaatgcgatt tctgctgcgg
     3121 ctgctgcagg tcaacatcaa actgttcctg atgcgcgtaa taatctgaat caggcgactg
     3181 ctgaagcatc acgcctgaaa acagtggcag ataatgccct taatacagcg aagaataagc
     3241 gaaaggaagc tatcgatgcc gtaccagtag caactcaggc tgagaaaaaa tatcaggatc
     3301 tgcagcaaag cattaaaggc ctcactctaa ataataacgg ccaatacggt acccaaaaat
     3361 gggaggttat cagttcgaat aaagagcatg accactgggg ataccggttc tatccatctg
     3421 ggatcacaaa agctcaggtt gatgcagcgc agaatgatgc ggtgaacaaa cgaaatgctg
     3481 ctaccagtct tgccagtcag gcaacggccg ctgagcaggc tagtctgcag gcatcagctg
     3541 cctataatgc tgcagaaacg cgccgtcagg ctgcccaggc tgcattagcg tccgcagaac
     3601 aagccgccgc tgctgaacgt aaacgccatg aggcggaagc agcagccgcc gctgcggcag
     3661 aaaaaaaacg acaggctgat gcagcagcga aagccgctga ggaggccaga gccattgctg
     3721 aaaaagcaaa agcgctgcag gcccgtcgta cagccgcaga taaactgaaa tcttcagaga
     3781 ttcaggctgt caggggtatc ccggctacag ctgcaccttt tgctattcct ctgacctggt
     3841 caactgcgtc acgtggaagt tttactctgg cggctgatgc tgcggccagc ctgggagctt
     3901 tcatcagcga ggctctggcg acgttacgtg tcgctgtggt ggctaacccg gtagcattaa
     3961 ccattgccgg tcttgtgtta tcgaaatcag ttggcgtcgg tagtgatatg gtgcccggtc
     4021 gggatatcag ttcgatgatg cctggcgatg cgtttggtct gcctgatacc gcggcattga
     4081 ataaagcagc agatcagaaa acatctgtca gtatgccggt tcgtggccgt ctggttatga
     4141 atgatagcgg cattctggat gttcagctgg tcaaaaccaa tactgccggt gccgtgaagg
     4201 tcgctcgtgc tgtactggat aaggaaaccg gctactgggg ctatactctt cccgctgtcg
     4261 cagatgttcc ggcgcaaact atttttgtca gcccggctga cgcacctggt gcaaacggac
     4321 cactgacgct aagcgggccg gtaccactac cggagcgtat tctgcatacc ggtgatcaga
     4381 tttcggcgcc acaggctaca gataaaaccg tgacgccggt agcggatgac ctcgattttg
     4441 acgatattat tctggtgttt cctccggaat ccggcctaaa accgctgtat gtgatgtacc
     4501 gtagccctcg taacatgccc ggtacagtga gcggcaaagg gcagagcgtt ggcaataact
     4561 ggatgggggg ggccagtacc ggggatggtg ctcctgttcc ttcccagatt gcggataaat
     4621 tacgcgggaa gactttcggt agttttgatt ctttccgtcg ggcgttctgg aaagcggttg
     4681 ctgacgattc tgcactgagc aagcaattta gtgaagctga tattaatcag atgaaagctg
     4741 gaagggcacc tactgcggac tttttagaat cagtagggaa acgtgttaaa attgaattac
     4801 accacgaaaa agaaatatct caaggagggg ctgttatgga tgtcgataac attaaggcgc
     4861 taacacctaa aaatcatatt gagacacata aaggaaaata aaatggctgg taataaaatt
     4921 tcagatttta cggaagaaga atttactgct tttgttagta aaataatcgc tgctgatttt
     4981 ccaactgaca aagagaacga tgatgctatt tattcgttcg cccaactaac agagcatcct
     5041 gctggttgga acctcatctt caagcctaag ataggcgaag attcttcggc gaaagggatt
     5101 gttaaaaccg taaaggaatg gcgagctgct aacggtaagc caggttttaa aaaagcgtga
     5161 ttgaatttgc gggttcaagt tatgagcccg ctcatttgta actctggatt tgaagaataa
     5221 ttactagata tccatttgcc tagaataata aaaataaaag gtgatatatg aaaaaattac
     5281 ttttcctagg ggttgttacc gtttctgtgg ttctggcagc atgtcaggtc aataatgttc
     5341 gagacaccgg aggtggggct gtttcacctt catcgacggt gaccggagtc acgcttgata
     5401 gcggtacgaa cggcaatccg taaaggatct tcttgagatc ccatttggat cgtcgtaatc
     5461 tcttgctctg taaacgaaaa aaccgccttg gcgggcggtt ttttcgaagg ttcgaggagt
     5521 tggcgctctt tgaaccgagg taactggctt agaggagcgc agtaaccaaa ttcgttcttt
     5581 cagtttagcc ttaactggca cataacttca agactaactc ctctaaatca gttaccagtg
     5641 gctgctgcca gtggcgcttt tgcatgcctt tccgggttgg actcaagatg acagttaccg
     5701 gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     5761 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     5821 aatgacaccg gtaaaccgaa
//
