LOCUS       NZ_CP144315            10141 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CRE-60 plasmid pKP60-2, complete
            sequence.
ACCESSION   NZ_CP144315
VERSION     NZ_CP144315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39712905
            Assembly: GCF_036542205.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Tran,T.T.D.
  TITLE     Hypervirulent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Tran,T.T.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2024) Institute of Microbiology and Immunology,
            National Yang Ming Chiao Tung University, Linong St, Taipei 112,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. Oct 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 22.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 23:07:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,007
            CDSs (total)                      :: 5,883
            Genes (coding)                    :: 5,658
            CDSs (with protein)               :: 5,658
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 140 of 225
            Pseudo Genes (incomplete)         :: 92 of 225
            Pseudo Genes (internal stop)      :: 24 of 225
            Pseudo Genes (multiple problems)  :: 31 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-60"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP60-2"
                     /geo_loc_name="Taiwan"
                     /lat_lon="25.04112423 N 121.51916648 E"
                     /collection_date="2017"
                     /collected_by="Yu-Tsung Huang"
     gene            complement(join(8511..10141,1..117))
                     /locus_tag="V3C89_RS01240"
                     /old_locus_tag="V3C89_01240"
                     /pseudo=""
     CDS             complement(join(8511..10141,1..117))
                     /locus_tag="V3C89_RS01240"
                     /old_locus_tag="V3C89_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            444..689
                     /locus_tag="V3C89_RS01245"
                     /old_locus_tag="V3C89_01245"
     CDS             444..689
                     /locus_tag="V3C89_RS01245"
                     /old_locus_tag="V3C89_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            963..1334
                     /gene="tolA"
                     /locus_tag="V3C89_RS01250"
                     /old_locus_tag="V3C89_01250"
     CDS             963..1334
                     /gene="tolA"
                     /locus_tag="V3C89_RS01250"
                     /old_locus_tag="V3C89_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1331..1549
                     /locus_tag="V3C89_RS01255"
                     /old_locus_tag="V3C89_01255"
     CDS             1331..1549
                     /locus_tag="V3C89_RS01255"
                     /old_locus_tag="V3C89_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            1891..2169
                     /locus_tag="V3C89_RS01260"
                     /old_locus_tag="V3C89_01260"
     CDS             1891..2169
                     /locus_tag="V3C89_RS01260"
                     /old_locus_tag="V3C89_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2157..2471
                     /locus_tag="V3C89_RS01265"
                     /old_locus_tag="V3C89_01265"
     CDS             2157..2471
                     /locus_tag="V3C89_RS01265"
                     /old_locus_tag="V3C89_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2635..3063
                     /locus_tag="V3C89_RS01270"
                     /old_locus_tag="V3C89_01270"
     CDS             2635..3063
                     /locus_tag="V3C89_RS01270"
                     /old_locus_tag="V3C89_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3089..3268
                     /locus_tag="V3C89_RS01275"
                     /old_locus_tag="V3C89_01275"
     CDS             3089..3268
                     /locus_tag="V3C89_RS01275"
                     /old_locus_tag="V3C89_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3296..3826)
                     /locus_tag="V3C89_RS01280"
                     /old_locus_tag="V3C89_01280"
     CDS             complement(3296..3826)
                     /locus_tag="V3C89_RS01280"
                     /old_locus_tag="V3C89_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3833..4564)
                     /gene="mobC"
                     /locus_tag="V3C89_RS01285"
                     /old_locus_tag="V3C89_01285"
     CDS             complement(3833..4564)
                     /gene="mobC"
                     /locus_tag="V3C89_RS01285"
                     /old_locus_tag="V3C89_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_032448355.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4564..5475)
                     /locus_tag="V3C89_RS01290"
                     /old_locus_tag="V3C89_01290"
     CDS             complement(4564..5475)
                     /locus_tag="V3C89_RS01290"
                     /old_locus_tag="V3C89_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_074421349.1"
                     /translation="MADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVK
                     GLITKLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQII
                     IARDEFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLP
                     ADFVKTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDS
                     FIQKEARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDP
                     LAEYQSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5495..6526)
                     /locus_tag="V3C89_RS01295"
                     /old_locus_tag="V3C89_01295"
     CDS             complement(5495..6526)
                     /locus_tag="V3C89_RS01295"
                     /old_locus_tag="V3C89_01295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_331708831.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLRPVRPEESSG
                     CRI"
                     /name="hypothetical protein"
     gene            complement(8010..8159)
                     /locus_tag="V3C89_RS01300"
                     /old_locus_tag="V3C89_01300"
     CDS             complement(8010..8159)
                     /locus_tag="V3C89_RS01300"
                     /old_locus_tag="V3C89_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8244..8501)
                     /locus_tag="V3C89_RS01305"
                     /old_locus_tag="V3C89_01305"
     CDS             complement(8244..8501)
                     /locus_tag="V3C89_RS01305"
                     /old_locus_tag="V3C89_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             3511..10141
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7328..7907
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.77"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3837..4564)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.74e-141"
ORIGIN
        1 accaccacct gatgaagggc cacctttacc agacgtaccg ttcacattcc cactggcctg
       61 accaccatta tgtcccagac ctgaattacc cggacctcga ccgtctccac cactcatatc
      121 gttttcctct ttgacattta aaacaataaa ttaaagccaa tatactgtac atatacacag
      181 tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt agttctcatc
      241 ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt tcccgaaaat
      301 ttctcacggc aacaaatttt attatttgag atgtacatca cacttacatt tcaggtggta
      361 tagacacatc tgaaagctaa ctggtatgag gggaggaccc gcggttaaac tgttcaggaa
      421 acctgagacg cccggaggcc accatgacac acctgaaact gactcccgct gaagaaaagc
      481 gcctagcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg aacagaacgc
      541 tgccaaaggc ggtaaaacag caggtgcggg aaagggtgct tcgcgccatt caggacgaac
      601 gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggcggcg gaaaccacct
      661 tcacctggca gccgctgcgc cgccggtgag gggtatttac cataaaaccc gttatcagca
      721 ccgcccgaga agccctcaga gcgtttccgg cggctacggg ataaaacagg caacaaatca
      781 cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc ctatgcacgc
      841 aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt atgtcaatga
      901 acgtggccgt aaagagtggc aaatatacat gtaatatttt ttactgacag gtcatcaacc
      961 cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc ctgacggcct
     1021 gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca tctaaaatca
     1081 gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa ccagaggcct
     1141 atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact ctgaacagcg
     1201 ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc gttacccacg
     1261 ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac gcccctctgg
     1321 atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc tcactccggt
     1381 acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg tacaggccgc
     1441 agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg atggacgagt
     1501 ggacaacgtg tcgcttaccg gctgtcactt ttttttcggg aaacgctgac ggcaatgagg
     1561 cactggctat atcccgcagg gcacccggca atgcgacagg ttataaattt tgaattcagt
     1621 ctgaaaaatg acggcttaca ggatccgcga ccagccacca ccaaaaagtt caatgatgat
     1681 ggttgccgcc agtaacaggt cctttaaccc gcaggctgca atagtcctgg atgatgagtg
     1741 agcctgtagc agccctgatt agtggaagcg aagctgaaaa atggggaagc gaagctgaaa
     1801 aatgggtagc aaaagccgta tttctggcaa atagcgccgc cacggattaa agtagtcata
     1861 tgtattacac atgactacaa aggggctttt atgtcatcag caacatccat caggctcgat
     1921 gaagaactta aggaccggct gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg
     1981 ctaatgctgg aggcaatcac cgaatacatc gaccgggaag agaagcgcag ccagtacctg
     2041 cgggacggac aagccgcatg gcagcactat caggaaacgg gtctgcacct tactgcggaa
     2101 gaagcagaag cctggataag tacctgggga actgagaacg agcaggatgc tccgccatgc
     2161 cacaggtaac gatttccgcg ctggcacagc gagatttaca acgcctccag gactttctca
     2221 aaaccaaaaa tcggctggca gccagaaaag ccggtgaggt gatcgtccgg gctatccagc
     2281 aactgaaaac attgccagac attggccgcc cggtgccatt tctgccgctg gaatatcagg
     2341 aactggtgat cgggtttggc gacagtggct atgtgatgct ctaccgccac gaccgggaaa
     2401 tggaccggat tgtgatcgtc accgtcaggc atcagaaaga atccgggtat ccgggggcag
     2461 acagcctcta ataaccgatg acgggggcgc aattcattgc gtggccgtcg ccggtttgac
     2521 aagcacagcg cgtcagaaaa ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac
     2581 atttaccgtg agaaattaca catctttcac acccgataaa atcaggggta tacagtggcg
     2641 aaaacattgc tcaacctttc acaggcagca caagccgcgg gcattaccag aagaacgcta
     2701 tacaatcatg taaaacaggg aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc
     2761 gtggatgtgt cagaactgat ccgtgtatac ggaaatgtga atatccctga gaaacaaatt
     2821 cccggcattt cacaccgtga aaatacacaa aaaaatttcc cacaggaaca gcttctggca
     2881 atgcaaaagg aactggccga tctccggcag gctgtcaccc tgatgctgga agataaaaca
     2941 tcccgagaag aagaacgccg ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg
     3001 gacagattga caacagaact gacacagaag aaaaaacgct tctggtccgg ctggtttagc
     3061 tgagaactta ccgaagggaa tggagaacat gacgaagcag gaaaaaaccg ccctcaatat
     3121 ggctaaattc attaaggacc agtcgctgct gttgctcgag aaactgaacg agctggatat
     3181 ggataccccg gccgacatgt gcgagcggct gcacgaggat gcggaaacac tctggcaggt
     3241 gatgcaggat aagctcgggg aagaataaca gcaccggcat ggccggtgct ggcgtttatt
     3301 ttttgccgaa cggccacctc cggcgggcgg gtttatcctc cggcagttct ggcaccggaa
     3361 cgttaaaata gcggctgtta tattcccgca tcagctccgc tttcagcttc tcgttcaccg
     3421 cgctttcccc gtaccgctca ctcacgacgt caatcatcgc cccgatctgc tccggggtgt
     3481 atttcccggc gtccagtacc tgcacgccgt tctcgatggc gaactgctca accttgcgat
     3541 aaagataatc acacagcttc attccggccg gtttctcacg ttcccacgcc ggatttttgc
     3601 cggtgaagta atacgtggct ttgtcatcgc tgttcagccg ttcgtgatac atgccgataa
     3661 acttcatcat tgccagatgc tgggcctgat cttcaaacct gaatacatat gcaccggtct
     3721 gccaggccat gaccgccatg tcttccagat gctcgctggt tggcataatg cagcgctcgc
     3781 cgtctttgtc ttccacgtcc tcaataaata ccatgtacag cgccataatc cctcactccg
     3841 ttgtcataaa tttaaagcat gcgaacagcc tctcccggtc cagctccgcc gttttgcggg
     3901 cctcgccggt ctgcgggttc acgatttcat ccaccgccag aatcgcccgc tgtacccgga
     3961 caagcgtcgg ccggtcaggg cagatgtacc agatttcatc gatgccgttc gccttacggt
     4021 tgaacagata cccggacacc acttcctgat accggcgacg gctcttgacg gtacgttcca
     4081 cctcaaacgc caccgttctg ccgtccagct ccgccagcgc gtccggcacc ttcatccctt
     4141 tcagggcaat acggtgcagg taccgccagc cggtggcccc ccgggcctcc atggcgaggc
     4201 gcagggactg cacggccagc cggtgcggca gctgcgccgc cgacacccgc gacggctgaa
     4261 acggaataat gtccgtcagc ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg
     4321 tcagccccca cacgctgacc tgacggccgt caaccgggta tttcgcccgg accagcagcc
     4381 ctttcgccac caggctgtcc aggaagcggt aaaccggctg taccgccgca atccccagcc
     4441 gttgcgcaac cagctccgcc gtggaacaca gttcttcccg cagccagcgc agggtcagcc
     4501 gtgttttctc cgcgttccgt gcctgccgct tcgcgtggct gacgttgtat cgctccagtg
     4561 ccatcagtac agccccgcaa aatcatcatc acccggccgc tgccgcccgg cctccgactg
     4621 gtactccgcc agcgggtccg ttttcgccag caccgggaag gttttcagcg gaatttcccg
     4681 gcgatcaaca cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc cggtcatcac
     4741 cgcgaacccg tcaggcagat gttgcagggt gttcacgtca aacagcggcc gcgcctcctt
     4801 ctggatgaag ctgtccccgc tgatatgctc cacgttcccc gcctcccggc tggcccggcg
     4861 gcgctccacg tccacccgga tctccccggt ctgtccggcc gcccactgag cggactcctg
     4921 gctggccgcc cggtagaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa
     4981 atcagcgggc agatcctgcc cgcactggcc gaagtcgccc agcgactggt gcgcgagcag
     5041 caggttgcag tcccggctgc gcagcgtccc cagcgcgttc agcacgtact tcgacagcag
     5101 atatttaatc tcatcgagca tgatgctcgc gtgcggccac caccggaact cgtcccgcgc
     5161 aatgatgatt tgcgcgcagc gggcaaacag catcttctgc acccggatca ccgcctcatc
     5221 gtccatcgag ccgatgacgt acaggcagcc gccgccgttg attatccccg ccacgtcgat
     5281 gccgctgtcc gtctgaagga cgctcaggtc ggccacgttc tccagcttgg tgatcagccc
     5341 cttcacgtcc tttttcaggg cctccggcag tgcgtacgcc gccgccagca cctgctgaat
     5401 atttgccccc tgcggaaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg
     5461 gtagtggtcc gccacgtcgc cggtctcact caggttaaat ccggcaacca gaagattctt
     5521 cagggcgtac tggtcgaaga tcgcggaaca gattcagctg cgccggtttc cccggccgca
     5581 gatccagcag ggtgaagtcc gggcagtgcg cccggaagac gtgcggcgcc cacgcgtccc
     5641 ctttcgggtc gaagtacacc accgcgtccc cgaaatcgcg tacgcagcga atgagcgcgt
     5701 tggtggccat cacgctcttg ccgctcccgg gcaggccgag gatctgaatg ttggtcttgc
     5761 gccaggtatc acgcgggacc agcaccggct ggccctgctc atccttaccc agaaacaccg
     5821 cctgctgttt ccgggcctgc tccgtggaaa aatcggccag cgcgttaacc ggtaccggct
     5881 ccccgaaatg caggtggcgg acgtcggtca tttctccggt ccgctggctg ctgcgccggc
     5941 tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc cgcaaaatgc ccgtcaagca
     6001 gcaggaacgc cagcagccgc gccaggagga cgcctgcgac aagacctccg gcacaccagg
     6061 caatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgttcgcg tagaggtccg
     6121 gacgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc ccccccagaa
     6181 gggccagacc ggcagcgccg gtcagccagt atgcgtagtt ccagacatgt cgccgtgccc
     6241 tgaccatccg cccgggcgaa tccagcgagg ccagcaccga aaatggcgca gtccggttat
     6301 tatcgcgctt atagcgatcg gcatactccc gccgctcctt atgactcagc cccacgaatc
     6361 gcatccgccc ggcccagagt tctgccagac cgaaaaacag gccgataacc agaaaaaaac
     6421 cggccagact gtaatactcc cggtacagcc agctgaccca cgggtaaatc tgccgggcca
     6481 gctccgggat atgccactgc caggcaagcg aatccgtatt aaacatgcaa tctctctcct
     6541 tcagacgacg ggtttaccgg cgtgttgtgc agcccctgct gcacaagccg cggacatgcc
     6601 ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc accgcggagg
     6661 ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac gagcacgccc
     6721 cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt ccggcccccg
     6781 tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc tttgctgctt
     6841 cgtcaggtgc gtccagcacc gtggattttg tgggtggtca ggcgtgagcc tgacgggggc
     6901 cgggtgaatt tctggcgtga ccggcgtcca gccggacaga gccagaaaga gggggaaaga
     6961 cagtgcgtcg gtggcgtcca gccagcgtaa gcaggggatt tccccctgaa gtcagtcacg
     7021 ccccagcggg gatgacgtgc atttctccgc aaggagaaaa aagcaacaga gataggttaa
     7081 cgtaaaatat tcagcagaaa accgacagac ttcacctatc tgaataattt atcggcaggg
     7141 aagagaaagg cggtaacacg gagcaatatg tcacatccac gcgtccggtg accgtcttgc
     7201 aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgcgccgg ccgtgagact
     7261 gcggcgggcg ctgcgaactc tcgcaaagtt acccacagat tccgtggata agcagggaac
     7321 gagcatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag gctccgccct
     7381 cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc
     7441 aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc caccgtttcc
     7501 ggtaggtcgc tccctcttgc gctctcctgt tcctgaccct gccgtttacc ggatacctgt
     7561 ccgccgttct cccttgcggg aagtgtggcg ctttctcata gctcacacac tggtatctcg
     7621 gcccggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc gttcagcccg
     7681 actgctgcgc cttatccggt aactgttcac ttgagtccaa cccgggaaag cacgcaaaac
     7741 gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg
     7801 aggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct
     7861 gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat
     7921 caaaccacct ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa
     7981 aggatctcaa gaagatcctt tgatcttttc tactgaaccg cgatccccgt cagttcagag
     8041 gaggacgatg gtgccaccgt tccgccctga acatcccgga tataatttgc ctgacatgcg
     8101 gccagaagaa agccagcaac aatcaatata aaaagaaaaa tagtctttac ctttttcata
     8161 tactttccct gtatttaaag actccggcat tcatccctga acaccggaag tcagtatttc
     8221 aaatcatcct tgataatttt ttgttaccag ttaccgtccc gataatcaaa agctatataa
     8281 caattatcca catcaggttt tatttgattt ttaaaaaatg gctgtagtaa aggaacccat
     8341 tctttcttca aatcaaactc accattatta atattatcct ttaaaggcat tcccaagctt
     8401 tcaatgacag aaccatcgtc accaaagtct ttagaatact cttccccttt aaactcttca
     8461 gttttcttat caaaccatgt caaacgtaat ttaagcccca tatttacctc tcaaagatat
     8521 tttttaatgt tccgttttgg atctggccct ttaacctgct tacccgtttt gggatcaaat
     8581 gccccgaggt gttcgccatc actggcacga tacccttcaa gctcaccgtg ctgggaatcc
     8641 cattcataaa ttttacgttt tttatcgcca taccatcggg cccgcttacc accaccacct
     8701 tgtttaggtg ttttaggatc acctcgcttt aactctccaa gtccctttat gtcttctgtt
     8761 ttaggtactg gatgataatc atggccgtag tctttagtcc ccttgcgagg cttgttcttt
     8821 tcctcattta acttattttc tgcatccttc gcttttttct ccttttgttt ccggctctcc
     8881 atcgccccgg taagtgcagc cttatctctg gataaggatt cattcattgc gtttaaagat
     8941 gcctgagccg cttttaattt ctcattctga ttctgaatat ccttatcaag atagcgaacc
     9001 tgatcctgcc acccggagcc atattcatga ggcgggttat aagtcacaaa atcatcttta
     9061 tttgcctctg cctcttttac ctttttatcg gcttcctgaa cagcagccct tgcaccgggg
     9121 agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg caatattttt atcttcggca
     9181 tccagctcag ctttcgcttt atcatagtct cttttcagcc cctcttccgg atgagcggcg
     9241 tcccatgcct gctggcgacg cttttcttcc tcctgacgct gttttacctg tgccggggta
     9301 agaacatctg tcaccgacac ataaaccggc ttctgtccgg tctctttcgg gaaacgaata
     9361 acggcctcac gggagttacc accggctgta aaaccagccg gacgtgaatc acctttttca
     9421 gcaacaatgc cttttggcgg ggctttcgct gtcggaacac ctttcggtac gctcacctgc
     9481 agagacggga gacccggaat cgacacactg aatacccccg gacgttttgt cggtttcgca
     9541 tccaccacag ggacactcat tggccgacct gcgacaaccg caatatgctg ccgctcatcc
     9601 ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg cctggtccag tggtaaagta
     9661 cttgccggag tctccgtcac cgcatcggcc ggtaatgacg tcataatttt tgacatcatt
     9721 ttcggatcat cttttgcaat ctcagaaggc agcacaccgt agatcgcaat cccccacaga
     9781 ccaaacttaa acggcccctt cagggcagcc agcacatcag caacggcggc cgacaacgca
     9841 tcgccggaaa ctgataaagc cagtccttca gcccccggag tagccagggc aggcagaccg
     9901 aaggccatcg cagtggccga agactgtccg ccaccagagc taccgctatg attgccgctg
     9961 ttaccaccat taccaccggg tttactcccg ccgttaccaa actcccccgg attatagtta
    10021 tgaatatctc cattcggcgt atgcgtcgta ccccagcccg gcccgctgtt tggatccgta
    10081 ccaccacctg atgaagggcc acctttacca gacgtaccgt tcacattccc actggcctga
    10141 c
//
