LOCUS       NZ_CP144256            11193 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter baumannii strain YFY3 plasmid pYFY3.1, complete
            sequence.
ACCESSION   NZ_CP144256
VERSION     NZ_CP144256.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36374922
            Assembly: GCF_038998165.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Li,M., Xu,A. and Wang,M.
  TITLE     Acinetobacter baumannii CG2 and ST164Pas/ST1418Ox population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   Li,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Department of Neurology, The Second
            Affiliated Hospital of Nanchang University, NO 1. Minde Road,
            Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038998165.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 03:59:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,872
            CDSs (total)                      :: 3,778
            Genes (coding)                    :: 3,693
            CDSs (with protein)               :: 3,693
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 28 of 85
            Pseudo Genes (incomplete)         :: 50 of 85
            Pseudo Genes (internal stop)      :: 13 of 85
            Pseudo Genes (multiple problems)  :: 6 of 85
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144256.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="YFY3"
                     /isolation_source="Nanchang"
                     /host="patient"
                     /db_xref="taxon:470"
                     /plasmid="pYFY3.1"
                     /geo_loc_name="China"
                     /lat_lon="28.6829 N 115.8582 E"
                     /collection_date="2021-10-08"
                     /collected_by="The First Affiliated Hospital of Nanchang
                     University"
     gene            complement(1..210)
                     /locus_tag="V3O07_RS19300"
                     /old_locus_tag="V3O07_19300"
     CDS             complement(1..210)
                     /locus_tag="V3O07_RS19300"
                     /old_locus_tag="V3O07_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(203..505)
                     /locus_tag="V3O07_RS19305"
                     /old_locus_tag="V3O07_19305"
     CDS             complement(203..505)
                     /locus_tag="V3O07_RS19305"
                     /old_locus_tag="V3O07_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(498..854)
                     /locus_tag="V3O07_RS19310"
                     /old_locus_tag="V3O07_19310"
     CDS             complement(498..854)
                     /locus_tag="V3O07_RS19310"
                     /old_locus_tag="V3O07_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1419..1718)
                     /locus_tag="V3O07_RS19315"
                     /old_locus_tag="V3O07_19315"
     CDS             complement(1419..1718)
                     /locus_tag="V3O07_RS19315"
                     /old_locus_tag="V3O07_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(1928..2446)
                     /locus_tag="V3O07_RS19320"
                     /old_locus_tag="V3O07_19320"
     CDS             complement(1928..2446)
                     /locus_tag="V3O07_RS19320"
                     /old_locus_tag="V3O07_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2524..3459)
                     /gene="repM"
                     /locus_tag="V3O07_RS19325"
                     /old_locus_tag="V3O07_19325"
     CDS             complement(2524..3459)
                     /gene="repM"
                     /locus_tag="V3O07_RS19325"
                     /old_locus_tag="V3O07_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            complement(4056..5225)
                     /locus_tag="V3O07_RS19330"
                     /old_locus_tag="V3O07_19330"
     CDS             complement(4056..5225)
                     /locus_tag="V3O07_RS19330"
                     /old_locus_tag="V3O07_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            5530..6291
                     /locus_tag="V3O07_RS19335"
                     /old_locus_tag="V3O07_19335"
     CDS             5530..6291
                     /locus_tag="V3O07_RS19335"
                     /old_locus_tag="V3O07_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            6408..6707
                     /locus_tag="V3O07_RS19340"
                     /old_locus_tag="V3O07_19340"
     CDS             6408..6707
                     /locus_tag="V3O07_RS19340"
                     /old_locus_tag="V3O07_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            6975..7262
                     /locus_tag="V3O07_RS19345"
                     /old_locus_tag="V3O07_19345"
     CDS             6975..7262
                     /locus_tag="V3O07_RS19345"
                     /old_locus_tag="V3O07_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            7249..7563
                     /locus_tag="V3O07_RS19350"
                     /old_locus_tag="V3O07_19350"
     CDS             7249..7563
                     /locus_tag="V3O07_RS19350"
                     /old_locus_tag="V3O07_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            7691..10102
                     /gene="znuD2"
                     /locus_tag="V3O07_RS19355"
                     /old_locus_tag="V3O07_19355"
     CDS             7691..10102
                     /gene="znuD2"
                     /locus_tag="V3O07_RS19355"
                     /old_locus_tag="V3O07_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            10407..10868
                     /locus_tag="V3O07_RS19360"
                     /old_locus_tag="V3O07_19360"
     CDS             10407..10868
                     /locus_tag="V3O07_RS19360"
                     /old_locus_tag="V3O07_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     Typing          complement(2525..3459)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4060..5147)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaagctatt tcgtaatatt ttatccaata agcattgtca aaaatatcaa tgtaatagct
       61 tggcctctta caatgtgaaa aatattcaca gcataaatct ctaataattt caatttcttc
      121 taggctatcg gcagcattga ctaaaaatat aaacttgtct aagttaacaa gtaatttagt
      181 actatttttc aaaggtttat tattagacat tgaaccctat gtgttttcct gttggaagct
      241 ctacatcaat acgaagtttt ccacccattg cttcaacata ctttttcatc gttgaaattt
      301 taagatcatg accacgattt tcgattgctg ataatgacgg ctgtttaatt ccaagggttg
      361 cagctagttc tttctgggaa atttctaact cttctcggat aaggtgaagc tgactttcta
      421 aaagtaattc atcagccatt ttttgaatac gagcttgact ctctggagag cgactagcca
      481 ataattcttg taagctctta gccattagat tgatctccta aagttgagag atgaagtgcg
      541 tattgctcat ctgcaattgc tagcatctct gtataaaaac gctttttatt actcttatca
      601 ccaatacaca gcacaatcgc ttgtcttagg ggatcgaatg caaaaaatgc ccttaacggt
      661 ttaccacgat gctgaacacg tagttccttc atattggtaa atttagaatc gtagacggta
      721 tctactaaag gacgacctaa actaggccct tgctgttgta gaacaaccaa tgcagctaag
      781 actttctctt gtgttgcttc atcttgctgt tcaagccatt cattaaacag atccgttgta
      841 atgactgtcc acatattaca atcacaatat agattacagt ctatatatta aatctgattg
      901 aatgactttg caaatggcaa taaaaaatcc cgacgtcatg tcgggatttt tcggtatgga
      961 gcgcataggt agggctgact acctgatggt catggtgcct gactgattat tattgttgtt
     1021 ttaagtcatt gaacatcctg tccatataga tcgatcataa gagcttcatc gggggataga
     1081 tacagacaat ttcgtgaaac gctgtaagcc aaaacgtaca aaaaataatc aaaatttatc
     1141 ccagtggtta tccacagaaa tagtggattc ttttgggtaa aaaagcttat aaactctatg
     1201 gaattgctat ttttgattat tttttaaaca attttattgg caagaagtat caaaatcgat
     1261 cataaaaaag ccgacttgtt tcaaagtcgg ctttttgggc atttttggat ttttatattt
     1321 ttattaagta gttgatattt aaattaatat acaagtgtac ttcgtataat atccattatg
     1381 ttaaatagta ggggactcta agtacaagat tagaaaattt agtctttaag aggagaaatt
     1441 tgttctagct tatcttcagg attattttct ctaatatgta taattcgatc ataattttct
     1501 actcctcgta agtgaggtgg tgcaaatgct ggatacttgt tgaaaaagtg cattttaata
     1561 tttagtgcat ggtctaaaat aaaagcacat tcaattccat agtacttatc attaataaaa
     1621 aaattgtctg tagtttctat tagttcttga atagctaaat ccaatttttg aattttttca
     1681 ttaatatctt tagttttctc ttctatcttt ttgtccattg gtatcaccta tagaattaag
     1741 tttccctaaa attaacataa tacaccttat acgaaattct ttgtaaaaag cattttaaaa
     1801 caatgtctta tggatgtaaa aacagcctaa gcagtcacgc tcaggctgtt ttagtgattt
     1861 tttatgtttc acgatcaatt attgatcatt gttccacggt tttgattagt ctgattaaat
     1921 agtcctatca tccatagggc ttgaaaaaac ggctccagaa gccccttttt tctggttctg
     1981 gctcaatatg ttcagggact ggaatacgct tattctcgat aacctctgac tccttaggag
     2041 gcatagctat gacgtcctct ttttcttcta ctgtggattc tgcctgtgtt gatccaggat
     2101 catcagattg tgttgtattg gtttttggag cttccaatag gcgttgcata gtttcaattt
     2161 gattttgata gaagctttct ctattttttg catcatctag ctgatttttt agtaggtcta
     2221 tctgctgtct aaggagtaaa acttctgccg aagttggact gtctactttt acagtttctt
     2281 ttacactctg tttttgtact ggctcaccaa atacccggat cgcttctgca agatcaatct
     2341 tattgtcaga atttttactt aaaatacctt tctttatatt gttgtaaatg gtttgtcgat
     2401 taatattgta aagcttagaa agctcaagaa ctgtaaggct tttcatgtgt aaatctctgc
     2461 ctaatcttgt aaaaaattag tgtctattag actgtctaat agacaaatca gcaagtctat
     2521 cttctatgca gctttgaagc ctactttttg gagataagga tagagttctt taaacttttc
     2581 tgggttttga agcatttctg caatgcgtac agcgaattgc ggatagcttt ctgtaccatg
     2641 agaatacttt cccatttcag gaagttctga cagtttgtgg gaaaacagat ggcgttgagc
     2701 atctgtcatt tttgagaaaa tatctatagt gtctgggtca cgcttatttt ttattggcag
     2761 attactcatt tttttctgtt tgaaagtgaa tgaaaaacct gaaatagaac gccctttttt
     2821 atgttgctca tatttagctg ttatatcggt taactcattt acctgcttaa gagcaaaatc
     2881 taaaacatat gttttaaagt tactcattgt tttgtattga tgaggtttga caccaagttg
     2941 ttgtctaaag atagatagat caaaaagtgg cgtttttccg gcagatctcc attgaattaa
     3001 tagctcgtat agccgaatag catatgagct acttaaccta gaaacctgct gcaattcata
     3061 tttagtgaaa ttttcttcaa gtcgagttat gagtggaacg atatcttggg tgaatcgtag
     3121 gaaaataatt cctgattgag attcataccc aattttgtcg acccaacgac aatgaaagct
     3181 acgatcctta cctgtcttag gattaatgtc atggtatgtg acatagcgat caaataagct
     3241 tttagatgcc tctctgagta cggtataagc ggtatgtttc tcgacattaa aagtatttat
     3301 ataactactt gcatgtactt ctaaaagact attttcagtt atcccatgtc cagtttctcg
     3361 agcttcagca atggctaata ggatcagtct ttgttcaact gtatctaagg tataacttgc
     3421 ttgaattaga gcattatcct ttacgattaa ttcactcatt tctaataata aatttataat
     3481 taaactaggt tgtacaatag actatttcaa ttgagttgta aattaactta attatgggtc
     3541 gataaaccta gctttaaggt cgataaacct agttttatgg gtcgataaac ctagttttat
     3601 gggtcgataa acctagtttt atgggtcgat aaacctagtt ttattaacta tgaaactatt
     3661 ataaataaag gtttacagct tatttaaaag catttaaaag attaaaaata attaaaagcc
     3721 caatgaatga aaaattgccc tatgttttcg ctacgctcaa actctattga acttcgctta
     3781 cgctcagttc gaaggggcaa tttttgggtt aatctcgtga taacttcaaa aaagcaaaag
     3841 caactcggaa ttcgcttcgc tcataagttt tttatactcg cttcgctcga cctagatgca
     3901 aattaaatct gttcgcaccc tccgggatgc tctgtatctc agtctcagca gcaaagggga
     3961 gtttgtaatc agggaaaaag catggcaaaa attttaattg tgtctgttcg ctcgtagaca
     4021 ctcgctctgt aaactttgga taaaaccaac tgagcttaaa aaccagggcc accgtaatca
     4081 taatcatcag ctttaggttg tactggttct gattgagtgc gtttgggagg cgtattttgt
     4141 atcggtaata cgtattcagg aagtgaatgc acctcgaact caaaacatgg ggtactgtta
     4201 cgtcttgagt cttctagttt ttcattcatt tcgtagatgg accaagcttc ttgtttaagt
     4261 atagggcctt ggtttttgat aatttcattc aaatcatttt ttagtttgtc cttatttgtt
     4321 gcattcttga ttaagtaatt caggtaatac atgacttcca atgatgaaaa tgggttgttt
     4381 tcagcactat aaaaatattc atgttgttga tctaattgct tttggcgttc ttcgagttct
     4441 cgttcttgct ggttgtgttc tctgattttt tggatttggg cgagatattc cgctgtcgca
     4501 ttttgttgtc caacgcctag ttttggctct ggaaggtata gcgtgctgtg gatctcgtta
     4561 tatttttcat agctgtctgc tgaaacctga tttttgatgg tgatgtcttg gccatccagt
     4621 gtgtaggtga cggtcgggca gtgctgtgca agggcatcat tcaaatgatt ttcccattgt
     4681 tttcggatat agatcaaaaa agtggaatag tcttcatggc catcttgaag ttggattttt
     4741 ttggcaccgc ctttggctgg atcttgaggg ttatagcgtt tgaagtactg ttctgcgctg
     4801 cgttcaatgc cgtcatccgc ttttagcgag aacatcagat gacaatgagg ctgatcttcg
     4861 ccactgatac gtgattttac gttgtgtatg gcgtatgagt gcggatattg ctgtggaact
     4921 atatgtttgt ctaaaaacgt ctctatgagc gttttacgct gttctgacgt gaactcattc
     4981 ggtagggcaa tttcatactc catgtaggcc ttggcattgg ctcgttcata cgtatctgct
     5041 gcctgccaaa aatcaatggg atcttttaca aaggcaggca tacatcgacc aggttcaata
     5101 tgttctatgg tctcattgct ggttttgcgg atgccttcat actgcatcag ccgggaaatg
     5161 tagagataat ggccggtcgc atagccctta tttttgcttt tgacgctgaa gtggtagctg
     5221 gccatgttca cctctgctgg gttgtgggtt cggtttttat gcagtgaaac ggaatgaaaa
     5281 ccgcccggat ttgaaatgcc tgtatggctg aaaaatccgg ttgaaggggg tcaaggggga
     5341 agcgccccat tgcgtactag tttctccgat gaggagaaac tgtcaagtgc gaattattta
     5401 atataaaaat aaaatttctt gaaaagaatt tttattttta tattaaataa ttattttcag
     5461 actgccctct ttaaatatgg taaaaccata tttaaagagg ttgcagttct gaaaatttgg
     5521 cggcagatga tggaaatgga aaaagcaaaa aagcgtggtc gacctgctca gttgttacag
     5581 attgctgagt tacatgcatt tgtagagttt ttagaacagc aagaacagct ttctgatctt
     5641 caatctcaag tgttaaaggc tttaaatagt gttgattgta attttgaggg gctaactcag
     5701 acagatcagg ttctggtcaa agaggcttta aaaccttatc gtgaacatct gaagttgaaa
     5761 ttgctgtttg aagagttgaa taatctgcct cttaaaaccg agtatgaaca gaagttttta
     5821 gatctgtatg aactttttca aaagaatgca ctagatcaga tggaactaaa tattttaaaa
     5881 accttggcga cacggtatct gaattttaaa gcgcaaaagc ttgaatacag tgatttagaa
     5941 ttgtatttga gtcagctaca gaagaaggat gctgggaaaa aacgcaaagc tgaaaatcaa
     6001 cgtaaatttg aattaggtgg tgctgttctt gtcgcattta agaaactcaa tattgatatt
     6061 agcaatgata ctcctcaaca gattaccaat cggattgtga atacgacaaa gtttcataat
     6121 gaagtacgta aatctttaat ttttaaagat gttaagacat atgaaaatga atattttaaa
     6181 gcaaataaac tttttattca ggtgttagaa ggattacata cctggcaaaa gggcggagaa
     6241 ttattgtcag taattgaaat taaaaaggcc ttagaaaaag gcgaagagta gtttatttag
     6301 cagggatttt agagaagttg gacatttcta ttttgggctt acattggaat ttcgcataag
     6361 gtgtattatg ttaattttag ggaaacttaa ttctataggt gataccaatg gacaaaaaga
     6421 tagaagagaa aactaaagat attaatgaaa aaattcaaaa attggattta gctattcaag
     6481 aactaataga aactacagac aattttttta ttaatgataa gtactatgga attgaatgtg
     6541 cttttatttt agaccatgca ctaaatatta aaatgcactt tttcaacaag tatccagcat
     6601 ttgcaccacc tcacttacga ggagtagaaa attatgatcg aattatacat attagagaaa
     6661 ataatcctga agataagcta gaacaaattt ctcctcttaa agactaaatt ttctaatctt
     6721 gtacttagag tcccctacta tttaacataa tggatattat acgaagtaca cttgtatatt
     6781 aatttaaata tcaactactt aataaaaata taaaaatcca aaaatgccca aaaagccgac
     6841 tttgaaacaa gtcggctttt ttatgatcga ttttgatagt ttttgccaat aaatctgact
     6901 aaaaattacg cttctcaact tgagtgatac aatttcgtga tacattattg tatatacaaa
     6961 tttgaatatt ttttatggaa cagtattttg aatgggatga ggctaagaat cgaaagaatc
     7021 agaaaaagca tgatatctct ttcgaaactg caagccttgt ttttgaagat ccattacgga
     7081 tctcaatcca agacagatat actaatggtg aagaacgttg gcaaaccatt ggacgagtta
     7141 aaggcgtact aatgttattg gtggctcaca ccatttttga tgaagatgac tgtgaaatca
     7201 tccgaatcat tagtgcaagg caagtcacta aagcggagcg aaacaaatat gagcatggtt
     7261 agatactcac acaaagagct gaatgagaaa tttggtgaga agcaagatgc tgaaattcaa
     7321 cgcttgcttg ctaaaggcac ggttcctgat gatcagctag atctatcaga tattcctgaa
     7381 attacagact ggagcaatgc tgtacgccaa aatcaatttt accgcccagt aaagcaacaa
     7441 acttcaattc ggttagatgc tgatgtactt gcttggttca aagcacaagg caaaggttat
     7501 caaacacgta tgaatgaaat tttacgagat gctatgctaa aagaattaaa aaatcatcaa
     7561 taagaatggg agcttaggct cccatttttg acttcatttt taggggggat taccattcaa
     7621 aaatttaaat agtagatttt ataaattttt aagtgttata ttataatata acattaaatt
     7681 tggttgtttt atgctaaata aaagtaaact atttttagct ttaattactt taggggcctc
     7741 gaaaatttta ttggcagctg aagggcctgt tactacctta aatacaatag tattaactgc
     7801 acaatctgat gagttagggt ctgagctgtt aggcaaaagt ctaaatgtta gtaatcagtt
     7861 tattgatact agtaaactta aacagcgttc gacaactcta ggggatgcct tgggtacgga
     7921 attaggaatt cattctaatc aatatggagg tggagcatct actcctatta ttcgtgggca
     7981 ggaaggaaaa cgtattaaag ttttacagaa taatgctgac gtgttggata tgtctaatat
     8041 gtctccagat cacgctgtca cagttgaacc aagtttagct aagtctattg aaattatacg
     8101 tggtgcaagt acccttttgt atagttcaaa tagtgcggca ggggtagtca atgtcattga
     8161 ctataaaatt ccaacccaaa tgcctcaaga cggattagag ggtaatacta cattacgttt
     8221 taatactggc agtaatgaga agttgaccac agcaggtgta acagttggct taagcccaca
     8281 tgtagcttta cgggcagaag ggttatatcg taatgctggt aactataaaa ctcctcatta
     8341 ccagtctagc tcttataatt ctttagaaga tcttgaaaac caaaacagta tttataaaaa
     8401 tttaaaatat ttaccagaaa gctgggcaga gtcgagagta ggtacgcttg gattatcctg
     8461 gatagacgat aatacttatt taggagtttc ttatacacat cgtcatgatg aatatggttt
     8521 accagcccat agccatttat atgaaggttg tggtgcctct gcaatcggta tcgattcacg
     8581 aattagtgga ttaaaaaatt atcttttata ttatcctcaa ttgatggatg agcaagatat
     8641 taattacata aatccacgtc ctgattgtca tcagcataat catattcatg aaactaattt
     8701 ctctcataat gctccttata ttgatttaaa tactcgacgt tatgatgtgc gtggagaatt
     8761 cactcaacca tttaccggta ttgacaaaat tcgtacctca ttgagttata tagattattt
     8821 tcacaatgaa ctcgagggag ataagattac taactttttt aaaaatacag gaaaagtagg
     8881 ccgcatagag ttatcacatc agccattagg ggaattaacc ggtatcttgg ggctacaata
     8941 tttggaacag gataatagtg ctttatcacc agtccattct caagaaggcc ataccaccta
     9001 tctagataat cagcagctat taaatcgaaa tgtcactaaa aactttagtg tatttggcct
     9061 agaaaaatat aactggaacg actttacttt cgaactaggt gctcgtatag aaaagcagaa
     9121 agttagtatg gattatgata tagaaaaaat taaagattca atgaaacctt ggcctaataa
     9181 atataacagt ccttatgtag aaaaaaataa taaaatcagg gctcaaaatt taaaatctat
     9241 tttagaagct gttcaaccta acaaagaaac agctttttct tatgcaggta cagtccactg
     9301 gagatttgct ccaaattata tcttatcatt aacaggtaca catcaagaaa gattacctaa
     9361 tgcacaagaa atgtatactc atggtatgca tctcgctact aattcttttg aaataggtaa
     9421 ccgctttcta agaaaagaga aatccaataa tttagaaatc agtctggcct ataaggatga
     9481 tttacttgat tatcaaatca gtacttacta ttatgatttt gataactata tatatttaca
     9541 gacactcaat gaagttttag gaactacaaa agttagagat cagcatacac tacgtattaa
     9601 tcattatagc cagtctgctg ctaattttta tggattagag ggcaacatag ggtatcaatt
     9661 taattctgtt tatcacggct ctctttttgg agattatgtg aaaggccgtt taacaaattt
     9721 acctgatgct gtaattgcat acgatatctg gaatagagaa ccgactttag ctccccaaaa
     9781 ggatcgttac actcctagat tacctcctgc tcgtttgggt acacgactaa aagctgattt
     9841 tgatgaatcg cttaaaggtg aaatagaata ttatcgagtc tttaaacaag ataatatttc
     9901 taaatttgag caggttacat caggttataa tatgcttaat atgactttag catataaaaa
     9961 taagctatct cacacagaat atgatttatt ctttaaagcg aataatttac ttgatcagaa
    10021 agtctatgcc catgaaacat ttttgccata tattccccaa attggaagaa attttagttt
    10081 aggcttaaat cttaatttct agataagata ctgtaatctt ctaagaaaat ttcttagaag
    10141 atttttctta taaaatgatt agattaataa ttaaggtaag cgtccgctga atcccatacc
    10201 tattttttaa ttcgatttag gtttccagca gtgtggcagt aactcttcaa tctgggtcac
    10261 tttatgtgtc ggcagccttt tcagcactga atagccaccg attttgtaga caccttttag
    10321 ctagataaaa agcaattttt ttgatcttta aagaatgagg atataagctg tttaaaactt
    10381 aattctaatt ggttaatagg tgaaaaatga aattagcaga agctctctta cttcgaagtg
    10441 atcaacaaaa aaaaattatt tcactaaaac aacgtatcaa tgcaaatgta ttggtacaag
    10501 atggtgatca accatctgaa gatccaaatg aactactaaa acaagtattt tctttaattc
    10561 aagaatttca aaaattaagt tatgcaattc atgaaactaa tgctttaaca aaattaaatg
    10621 atggccgttc tctacttgct ttattgactt tgcgtgatga atttgtagaa cagcacaaaa
    10681 ccctaactgc agcaataagt aatacatctc gtgaatctga tcgttatagt acgcgggaaa
    10741 taaagtggca taaagttatt ccagtttcat cattacaaaa acaagctgat gatattagtc
    10801 ttaaattacg tgatttaaac gtgttaattc agtcgactaa ttggaaaatt gatttgattg
    10861 aagcctaata gtcctttaga tataatcaag ttgaacatac tgggcgagta caagatccct
    10921 tacttccaca acataggatt gtaaataaag tgtagtggct tgcaaagcaa gtggataagc
    10981 ttaaaatttt aagtacaaat caatccatca cttggcataa ctcaaatctt tgtgcaaaca
    11041 aagttacacg ttacttataa ctaccatgta ctgacagtat gttcacctca attactaaaa
    11101 ataaagaaat gagcaagcat cagctcaact acatttctat ttttaattca tttcggcatt
    11161 taacataaaa tctcttatgc gaatctagaa gga
//
