LOCUS       NZ_CP144254            11149 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter baumannii strain YFY27 plasmid pYFY27.2, complete
            sequence.
ACCESSION   NZ_CP144254
VERSION     NZ_CP144254.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36374919
            Assembly: GCF_038995905.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Li,M., Xu,A. and Wang,M.
  TITLE     Acinetobacter baumannii CG2 and ST164Pas/ST1418Ox population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Li,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Department of Neurology, The Second
            Affiliated Hospital of Nanchang University, NO 1. Minde Road,
            Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038995905.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 04:21:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,893
            CDSs (total)                      :: 3,796
            Genes (coding)                    :: 3,719
            CDSs (with protein)               :: 3,719
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 23 of 77
            Pseudo Genes (incomplete)         :: 46 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 7 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144254.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="YFY27"
                     /isolation_source="Nanchang"
                     /host="patient"
                     /db_xref="taxon:470"
                     /plasmid="pYFY27.2"
                     /geo_loc_name="China"
                     /lat_lon="28.6829 N 115.8582 E"
                     /collection_date="2021-10-10"
                     /collected_by="The First Affiliated Hospital of Nanchang
                     University"
     gene            1..462
                     /locus_tag="V3O03_RS19405"
                     /old_locus_tag="V3O03_19405"
     CDS             1..462
                     /locus_tag="V3O03_RS19405"
                     /old_locus_tag="V3O03_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(788..997)
                     /locus_tag="V3O03_RS19410"
                     /old_locus_tag="V3O03_19410"
     CDS             complement(788..997)
                     /locus_tag="V3O03_RS19410"
                     /old_locus_tag="V3O03_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(990..1292)
                     /locus_tag="V3O03_RS19415"
                     /old_locus_tag="V3O03_19415"
     CDS             complement(990..1292)
                     /locus_tag="V3O03_RS19415"
                     /old_locus_tag="V3O03_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1285..1641)
                     /locus_tag="V3O03_RS19420"
                     /old_locus_tag="V3O03_19420"
     CDS             complement(1285..1641)
                     /locus_tag="V3O03_RS19420"
                     /old_locus_tag="V3O03_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2206..2505)
                     /locus_tag="V3O03_RS19425"
                     /old_locus_tag="V3O03_19425"
     CDS             complement(2206..2505)
                     /locus_tag="V3O03_RS19425"
                     /old_locus_tag="V3O03_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(2622..3383)
                     /locus_tag="V3O03_RS19430"
                     /old_locus_tag="V3O03_19430"
     CDS             complement(2622..3383)
                     /locus_tag="V3O03_RS19430"
                     /old_locus_tag="V3O03_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            3688..4857
                     /locus_tag="V3O03_RS19435"
                     /old_locus_tag="V3O03_19435"
     CDS             3688..4857
                     /locus_tag="V3O03_RS19435"
                     /old_locus_tag="V3O03_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            5410..6345
                     /gene="repM"
                     /locus_tag="V3O03_RS19440"
                     /old_locus_tag="V3O03_19440"
     CDS             5410..6345
                     /gene="repM"
                     /locus_tag="V3O03_RS19440"
                     /old_locus_tag="V3O03_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            6423..6941
                     /locus_tag="V3O03_RS19445"
                     /old_locus_tag="V3O03_19445"
     CDS             6423..6941
                     /locus_tag="V3O03_RS19445"
                     /old_locus_tag="V3O03_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            7151..7450
                     /locus_tag="V3O03_RS19450"
                     /old_locus_tag="V3O03_19450"
     CDS             7151..7450
                     /locus_tag="V3O03_RS19450"
                     /old_locus_tag="V3O03_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            7718..8005
                     /locus_tag="V3O03_RS19455"
                     /old_locus_tag="V3O03_19455"
     CDS             7718..8005
                     /locus_tag="V3O03_RS19455"
                     /old_locus_tag="V3O03_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            7992..8306
                     /locus_tag="V3O03_RS19460"
                     /old_locus_tag="V3O03_19460"
     CDS             7992..8306
                     /locus_tag="V3O03_RS19460"
                     /old_locus_tag="V3O03_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            8434..10845
                     /gene="znuD2"
                     /locus_tag="V3O03_RS19465"
                     /old_locus_tag="V3O03_19465"
     CDS             8434..10845
                     /gene="znuD2"
                     /locus_tag="V3O03_RS19465"
                     /old_locus_tag="V3O03_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     Typing          5411..6345
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3767..4854
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaattag cagaagctct cttacttcga agtgatcaac aaaaaaaaat tatttcacta
       61 aaacaacgta tcaatgcaaa tgtattggta caagatggtg atcaaccatc tgaagatcca
      121 aatgaactac taaaacaagt attttcttta attcaagaat ttcaaaaatt aagttatgca
      181 attcatgaaa ctaatgcttt aacaaaatta aatgatggcc gttctctact tgctttattg
      241 actttgcgtg atgaatttgt agaacagcac aaaaccctaa ctgcagcaat aagtaataca
      301 tctcgtgaat ctgatcgtta tagtacgcgg gaaataaagt ggcataaagt tattccagtt
      361 tcatcattac aaaaacaagc tgatgatatt agtcttaaat tacgtgattt aaacgtgtta
      421 attcagtcga ctaattggaa aattgatttg attgaagcct aatagtcctt tagatataat
      481 caagttgaac atactgggcg agtacaagat cccttacttc cacaacatag gattgtaaat
      541 aaagtgtagt ggcttgcaaa gcaagtggat aagcttaaaa ttttaagtac aaatcaatcc
      601 atcacttggc ataactcaaa tctttgtgca aacaaagtta cacgttactt ataactacca
      661 tgtactgaca gtatgttcac ctcaattact aaaaataaag aaatgagcaa gcatcagctc
      721 aactacattt ctatttttaa ttcatttcgg catttaacat aaaatctctt atgcgaatct
      781 agaaggatta agctatttcg taatatttta tccaataagc attgtcaaaa atatcaatgt
      841 aatagcttgg cctcttacaa tgtgaaaaat attcacagca taaatctcta ataatttcaa
      901 tttcttctag gctatcggca gcattgacta aaaatataaa cttgtctaag ttaacaagta
      961 atttagtact atttttcaaa ggtttattat tagacattga accctatgtg ttttcctgtt
     1021 ggaagctcta catcaatacg aagttttcca cccattgctt caacatactt tttcatcgtt
     1081 gaaattttaa gatcatgacc acgattttcg attgctgata atgacggctg tttaattcca
     1141 agggttgcag ctagttcttt ctgggaaatt tctaactctt ctcggataag gtgaagctga
     1201 ctttctaaaa gtaattcatc agccattttt tgaatacgag cttgactctc tggagagcga
     1261 ctagccaata attcttgtaa gctcttagcc attagattga tctcctaaag ttgagagatg
     1321 aagtgcgtat tgctcatctg caattgctag catctctgta taaaaacgct ttttattact
     1381 cttatcacca atacacagca caatcgcttg tcttagggga tcgaatgcaa aaaatgccct
     1441 taacggttta ccacgatgct gaacacgtag ttccttcata ttggtaaatt tagaatcgta
     1501 gacggtatct actaaaggac gacctaaact aggcccttgc tgttgtagaa caaccaatgc
     1561 agctaagact ttctcttgtg ttgcttcatc ttgctgttca agccattcat taaacagatc
     1621 cgttgtaatg actgtccaca tattacaatc acaatataga ttacagtcta tatattaaat
     1681 ctgattgaat gactttgcaa atggcaataa aaaatcccga cgtcatgtcg ggatttttcg
     1741 gtatggagcg cataggtagg gctgactacc tgatggtcat ggtgcctgac tgattattat
     1801 tgttgtttta agtcattgaa catcctgtcc atatagatcg atcataagag cttcatcggg
     1861 ggatagatac agacaatttc gtgaaacgct gtaagccaaa acgtacaaaa aataatcaaa
     1921 atttatccca gtggttatcc acagaaatag tggattcttt tgggtaaaaa agcttataaa
     1981 ctctatggaa ttgctatttt tgattatttt ttaaacaatt ttattggcaa gaagtatcaa
     2041 aatcgatcat aaaaaagccg acttgtttca aagtcggctt tttgggcatt tttggatttt
     2101 tatattttta ttaagtagtt gatatttaaa ttaatataca agtgtacttc gtataatatc
     2161 cattatgtta aatagtaggg gactctaagt acaagattag aaaatttagt ctttaagagg
     2221 agaaatttgt tctagcttat cttcaggatt attttctcta atatgtataa ttcgatcata
     2281 attttctact cctcgtaagt gaggtggtgc aaatgctgga tacttgttga aaaagtgcat
     2341 tttaatattt agtgcatggt ctaaaataaa agcacattca attccatagt acttatcatt
     2401 aataaaaaaa ttgtctgtag tttctattag ttcttgaata gctaaatcca atttttgaat
     2461 tttttcatta atatctttag ttttctcttc tatctttttg tccattggta tcacctatag
     2521 aattaagttt ccctaaaatt aacataatac accttatgcg aaattccaat gtaagcccaa
     2581 aatagaaatg tccaacttct ctaaaatccc tgctaaataa actactcttc gcctttttct
     2641 aaggcctttt taatttcaat tactgacaat aattctccgc ccttttgcca ggtatgtaat
     2701 ccttctaaca cctgaataaa aagtttattt gctttaaaat attcattttc atatgtctta
     2761 acatctttaa aaattaaaga tttacgtact tcattatgaa actttgtcgt attcacaatc
     2821 cgattggtaa tctgttgagg agtatcattg ctaatatcaa tattgagttt cttaaatgcg
     2881 acaagaacag caccacctaa ttcaaattta cgttgatttt cagctttgcg ttttttccca
     2941 gcatccttct tctgtagctg actcaaatac aattctaaat cactgtattc aagcttttgc
     3001 gctttaaaat tcagataccg tgtcgccaag gtttttaaaa tatttagttc catctgatct
     3061 agtgcattct tttgaaaaag ttcatacaga tctaaaaact tctgttcata ctcggtttta
     3121 agaggcagat tattcaactc ttcaaacagc aatttcaact tcagatgttc acgataaggt
     3181 tttaaagcct ctttgaccag aacctgatct gtctgagtta gcccctcaaa attacaatca
     3241 acactattta aagcctttaa cacttgagat tgaagatcag aaagctgttc ttgctgttct
     3301 aaaaactcta caaatgcatg taactcagca atctgtaaca actgagcagg tcgaccacgc
     3361 ttttttgctt tttccatttc catcatctgc cgccaaattt tcagaactgc aacctcttta
     3421 aatatggttt taccatattt aaagagggca gtctgaaaat aattatttaa tataaaaata
     3481 aaaattcttt tcaagaaatt ttatttttat attaaataat tcgcacttga cagtttctcc
     3541 tcatcggaga aactagtacg caatggggcg cttccccctt gacccccttc aaccggattt
     3601 ttcagccata caggcatttc aaatccgggc ggttttcatt ccgtttcact gcataaaaac
     3661 cgaacccaca acccagcaga ggtgaacatg gccagctacc acttcagcgt caaaagcaaa
     3721 aataagggct atgcgaccgg ccattatctc tacatttccc ggctgatgca gtatgaaggc
     3781 atccgcaaaa ccagcaatga gaccatagaa catattgaac ctggtcgatg tatgcctgcc
     3841 tttgtaaaag atcccattga tttttggcag gcagcagata cgtatgaacg agccaatgcc
     3901 aaggcctaca tggagtatga aattgcccta ccgaatgagt tcacgtcaga acagcgtaaa
     3961 acgctcatag agacgttttt agacaaacat atagttccac agcaatatcc gcactcatac
     4021 gccatacaca acgtaaaatc acgtatcagt ggcgaagatc agcctcattg tcatctgatg
     4081 ttctcgctaa aagcggatga cggcattgaa cgcagcgcag aacagtactt caaacgctat
     4141 aaccctcaag atccagccaa aggcggtgcc aaaaaaatcc aacttcaaga tggccatgaa
     4201 gactattcca cttttttgat ctatatccga aaacaatggg aaaatcattt gaatgatgcc
     4261 cttgcacagc actgcccgac cgtcacctac acactggatg gccaagacat caccatcaaa
     4321 aatcaggttt cagcagacag ctatgaaaaa tataacgaga tccacagcac gctatacctt
     4381 ccagagccaa aactaggcgt tggacaacaa aatgcgacag cggaatatct cgcccaaatc
     4441 caaaaaatca gagaacacaa ccagcaagaa cgagaactcg aagaacgcca aaagcaatta
     4501 gatcaacaac atgaatattt ttatagtgct gaaaacaacc cattttcatc attggaagtc
     4561 atgtattacc tgaattactt aatcaagaat gcaacaaata aggacaaact aaaaaatgat
     4621 ttgaatgaaa ttatcaaaaa ccaaggccct atacttaaac aagaagcttg gtccatctac
     4681 gaaatgaatg aaaaactaga agactcaaga cgtaacagta ccccatgttt tgagttcgag
     4741 gtgcattcac ttcctgaata cgtattaccg atacaaaata cgcctcccaa acgcactcaa
     4801 tcagaaccag tacaacctaa agctgatgat tatgattacg gtggccctgg tttttaagct
     4861 cagttggttt tatccaaagt ttacagagcg agtgtctacg agcgaacaga cacaattaaa
     4921 atttttgcca tgctttttcc ctgattacaa actccccttt gctgctgaga ctgagataca
     4981 gagcatcccg gagggtgcga acagatttaa tttgcatcta ggtcgagcga agcgagtata
     5041 aaaaacttat gagcgaagcg aattccgagt tgcttttgct tttttgaagt tatcacgaga
     5101 ttaacccaaa aattgcccct tcgaactgag cgtaagcgaa gttcaataga gtttgagcgt
     5161 agcgaaaaca tagggcaatt tttcattcat tgggctttta attattttta atcttttaaa
     5221 tgcttttaaa taagctgtaa acctttattt ataatagttt catagttaat aaaactaggt
     5281 ttatcgaccc ataaaactag gtttatcgac cttaaagcta ggtttatcga cccataatta
     5341 agttaattta caactcaatt gaaatagtct attgtacaac ctagtttaat tataaattta
     5401 ttattagaaa tgagtgaatt aatcgtaaag gataatgctc taattcaagc aagttatacc
     5461 ttagatacag ttgaacaaag actgatccta ttagccattg ctgaagctcg agaaactgga
     5521 catgggataa ctgaaaatag tcttttagaa gtacatgcaa gtagttatat aaatactttt
     5581 aatgtcgaga aacataccgc ttataccgta ctcagagagg catctaaaag cttatttgat
     5641 cgctatgtca cataccatga cattaatcct aagacaggta aggatcgtag ctttcattgt
     5701 cgttgggtcg acaaaattgg gtatgaatct caatcaggaa ttattttcct acgattcacc
     5761 caagatatcg ttccactcat aactcgactt gaagaaaatt tcactaaata tgaattgcag
     5821 caggtttcta ggttaagtag ctcatatgct attcggctat acgagctatt aattcaatgg
     5881 agatctgccg gaaaaacgcc actttttgat ctatctatct ttagacaaca acttggtgtc
     5941 aaacctcatc aatacaaaac aatgagtaac tttaaaacat atgttttaga ttttgctctt
     6001 aagcaggtaa atgagttaac cgatataaca gctaaatatg agcaacataa aaaagggcgt
     6061 tctatttcag gtttttcatt cactttcaaa cagaaaaaaa tgagtaatct gccaataaaa
     6121 aataagcgtg acccagacac tatagatatt ttctcaaaaa tgacagatgc tcaacgccat
     6181 ctgttttccc acaaactgtc agaacttcct gaaatgggaa agtattctca tggtacagaa
     6241 agctatccgc aattcgctgt acgcattgca gaaatgcttc aaaacccaga aaagtttaaa
     6301 gaactctatc cttatctcca aaaagtaggc ttcaaagctg catagaagat agacttgctg
     6361 atttgtctat tagacagtct aatagacact aattttttac aagattaggc agagatttac
     6421 acatgaaaag ccttacagtt cttgagcttt ctaagcttta caatattaat cgacaaacca
     6481 tttacaacaa tataaagaaa ggtattttaa gtaaaaattc tgacaataag attgatcttg
     6541 cagaagcgat ccgggtattt ggtgagccag tacaaaaaca gagtgtaaaa gaaactgtaa
     6601 aagtagacag tccaacttcg gcagaagttt tactccttag acagcagata gacctactaa
     6661 aaaatcagct agatgatgca aaaaatagag aaagcttcta tcaaaatcaa attgaaacta
     6721 tgcaacgcct attggaagct ccaaaaacca atacaacaca atctgatgat cctggatcaa
     6781 cacaggcaga atccacagta gaagaaaaag aggacgtcat agctatgcct cctaaggagt
     6841 cagaggttat cgagaataag cgtattccag tccctgaaca tattgagcca gaaccagaaa
     6901 aaaggggctt ctggagccgt tttttcaagc cctatggatg ataggactat ttaatcagac
     6961 taatcaaaac cgtggaacaa tgatcaataa ttgatcgtga aacataaaaa atcactaaaa
     7021 cagcctgagc gtgactgctt aggctgtttt tacatccata agacattgtt ttaaaatgct
     7081 ttttacaaag aatttcgtat aaggtgtatt atgttaattt tagggaaact taattctata
     7141 ggtgatacca atggacaaaa agatagaaga gaaaactaaa gatattaatg aaaaaattca
     7201 aaaattggat ttagctattc aagaactaat agaaactaca gacaattttt ttattaatga
     7261 taagtactat ggaattgaat gtgcttttat tttagaccat gcactaaata ttaaaatgca
     7321 ctttttcaac aagtatccag catttgcacc acctcactta cgaggagtag aaaattatga
     7381 tcgaattata catattagag aaaataatcc tgaagataag ctagaacaaa tttctcctct
     7441 taaagactaa attttctaat cttgtactta gagtccccta ctatttaaca taatggatat
     7501 tatacgaagt acacttgtat attaatttaa atatcaacta cttaataaaa atataaaaat
     7561 ccaaaaatgc ccaaaaagcc gactttgaaa caagtcggct tttttatgat cgattttgat
     7621 agtttttgcc aataaatctg actaaaaatt acgcttctca acttgagtga tacaatttcg
     7681 tgatacatta ttgtatatac aaatttgaat attttttatg gaacagtatt ttgaatggga
     7741 tgaggctaag aatcgaaaga atcagaaaaa gcatgatatc tctttcgaaa ctgcaagcct
     7801 tgtttttgaa gatccattac ggatctcaat ccaagacaga tatactaatg gtgaagaacg
     7861 ttggcaaacc attggacgag ttaaaggcgt actaatgtta ttggtggctc acaccatttt
     7921 tgatgaagat gactgtgaaa tcatccgaat cattagtgca aggcaagtca ctaaagcgga
     7981 gcgaaacaaa tatgagcatg gttagatact cacacaaaga gctgaatgag aaatttggtg
     8041 agaagcaaga tgctgaaatt caacgcttgc ttgctaaagg cacggttcct gatgatcagc
     8101 tagatctatc agatattcct gaaattacag actggagcaa tgctgtacgc caaaatcaat
     8161 tttaccgccc agtaaagcaa caaacttcaa ttcggttaga tgctgatgta cttgcttggt
     8221 tcaaagcaca aggcaaaggt tatcaaacac gtatgaatga aattttacga gatgctatgc
     8281 taaaagaatt aaaaaatcat caataagaat gggagcttag gctcccattt ttgacttcat
     8341 ttttaggggg gattaccatt caaaaattta aatagtagat tttataaatt tttaagtgtt
     8401 atattataat ataacattaa atttggttgt tttatgctaa ataaaagtaa actattttta
     8461 gctttaatta ctttaggggc ctcgaaaatt ttattggcag ctgaagggcc tgttactacc
     8521 ttaaatacaa tagtattaac tgcacaatct gatgagttag ggtctgagct gttaggcaaa
     8581 agtctaaatg ttagtaatca gtttattgat actagtaaac ttaaacagcg ttcgacaact
     8641 ctaggggatg ccttgggtac ggaattagga attcattcta atcaatatgg aggtggagca
     8701 tctactccta ttattcgtgg gcaggaagga aaacgtatta aagttttaca gaataatgct
     8761 gacgtgttgg atatgtctaa tatgtctcca gatcacgctg tcacagttga accaagttta
     8821 gctaagtcta ttgaaattat acgtggtgca agtacccttt tgtatagttc aaatagtgcg
     8881 gcaggggtag tcaatgtcat tgactataaa attccaaccc aaatgcctca agacggatta
     8941 gagggtaata ctacattacg ttttaatact ggcagtaatg agaagttgac cacagcaggt
     9001 gtaacagttg gcttaagccc acatgtagct ttacgggcag aagggttata tcgtaatgct
     9061 ggtaactata aaactcctca ttaccagtct agctcttata attctttaga agatcttgaa
     9121 aaccaaaaca gtatttataa aaatttaaaa tatttaccag aaagctgggc agagtcgaga
     9181 gtaggtacgc ttggattatc ctggatagac gataatactt atttaggagt ttcttataca
     9241 catcgtcatg atgaatatgg tttaccagcc catagccatt tatatgaagg ttgtggtgcc
     9301 tctgcaatcg gtatcgattc acgaattagt ggattaaaaa attatctttt atattatcct
     9361 caattgatgg atgagcaaga tattaattac ataaatccac gtcctgattg tcatcagcat
     9421 aatcatattc atgaaactaa tttctctcat aatgctcctt atattgattt aaatactcga
     9481 cgttatgatg tgcgtggaga attcactcaa ccatttaccg gtattgacaa aattcgtacc
     9541 tcattgagtt atatagatta ttttcacaat gaactcgagg gagataagat tactaacttt
     9601 tttaaaaata caggaaaagt aggccgcata gagttatcac atcagccatt aggggaatta
     9661 accggtatct tggggctaca atatttggaa caggataata gtgctttatc accagtccat
     9721 tctcaagaag gccataccac ctatctagat aatcagcagc tattaaatcg aaatgtcact
     9781 aaaaacttta gtgtatttgg cctagaaaaa tataactgga acgactttac tttcgaacta
     9841 ggtgctcgta tagaaaagca gaaagttagt atggattatg atatagaaaa aattaaagat
     9901 tcaatgaaac cttggcctaa taaatataac agtccttatg tagaaaaaaa taataaaatc
     9961 agggctcaaa atttaaaatc tattttagaa gctgttcaac ctaacaaaga aacagctttt
    10021 tcttatgcag gtacagtcca ctggagattt gctccaaatt atatcttatc attaacaggt
    10081 acacatcaag aaagattacc taatgcacaa gaaatgtata ctcatggtat gcatctcgct
    10141 actaattctt ttgaaatagg taaccgcttt ctaagaaaag agaaatccaa taatttagaa
    10201 atcagtctgg cctataagga tgatttactt gattatcaaa tcagtactta ctattatgat
    10261 tttgataact atatatattt acagacactc aatgaagttt taggaactac aaaagttaga
    10321 gatcagcata cactacgtat taatcattat agccagtctg ctgctaattt ttatggatta
    10381 gagggcaaca tagggtatca atttaattct gtttatcacg gctctctttt tggagattat
    10441 gtgaaaggcc gtttaacaaa tttacctgat gctgtaattg catacgatat ctggaataga
    10501 gaaccgactt tagctcccca aaaggatcgt tacactccta gattacctcc tgctcgtttg
    10561 ggtacacgac taaaagctga ttttgatgaa tcgcttaaag gtgaaataga atattatcga
    10621 gtctttaaac aagataatat ttctaaattt gagcaggtta catcaggtta taatatgctt
    10681 aatatgactt tagcatataa aaataagcta tctcacacag aatatgattt attctttaaa
    10741 gcgaataatt tacttgatca gaaagtctat gcccatgaaa catttttgcc atatattccc
    10801 caaattggaa gaaattttag tttaggctta aatcttaatt tctagataag atactgtaat
    10861 cttctaagaa aatttcttag aagatttttc ttataaaatg attagattaa taattaaggt
    10921 aagcgtccgc tgaatcccat acctattttt taattcgatt taggtttcca gcagtgtggc
    10981 agtaactctt caatctgggt cactttatgt gtcggcagcc ttttcagcac tgaatagcca
    11041 ccgattttgt agacaccttt tagctagata aaaagcaatt tttttgatct ttaaagaatg
    11101 aggatataag ctgtttaaaa cttaattcta attggttaat aggtgaaaa
//
