LOCUS       NZ_CP144241            11194 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Acinetobacter baumannii strain SRM25 plasmid pSRM25.1, complete
            sequence.
ACCESSION   NZ_CP144241
VERSION     NZ_CP144241.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36374880
            Assembly: GCF_038987955.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Li,M., Xu,A. and Wang,M.
  TITLE     Acinetobacter baumannii CG2 and ST164Pas/ST1418Ox population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11194)
  AUTHORS   Li,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Department of Neurology, The Second
            Affiliated Hospital of Nanchang University, NO 1. Minde Road,
            Nanchang, Jiangxi 330006, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038987955.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 04:03:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,035
            CDSs (total)                      :: 3,939
            Genes (coding)                    :: 3,826
            CDSs (with protein)               :: 3,826
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 38 of 113
            Pseudo Genes (incomplete)         :: 68 of 113
            Pseudo Genes (internal stop)      :: 14 of 113
            Pseudo Genes (multiple problems)  :: 7 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144241.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="SRM25"
                     /isolation_source="Nanchang"
                     /host="patient"
                     /db_xref="taxon:470"
                     /plasmid="pSRM25.1"
                     /geo_loc_name="China"
                     /lat_lon="28.6829 N 115.8582 E"
                     /collection_date="2021-12-20"
                     /collected_by="Jiangxi Provincial People's Hospital"
     gene            complement(1..210)
                     /locus_tag="V3O01_RS20115"
                     /old_locus_tag="V3O01_20120"
     CDS             complement(1..210)
                     /locus_tag="V3O01_RS20115"
                     /old_locus_tag="V3O01_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069474.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCEYFS
                     HCKRPSYYIDIFDNAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(203..505)
                     /locus_tag="V3O01_RS20120"
                     /old_locus_tag="V3O01_20125"
     CDS             complement(203..505)
                     /locus_tag="V3O01_RS20120"
                     /old_locus_tag="V3O01_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001129974.1"
                     /translation="MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(498..854)
                     /locus_tag="V3O01_RS20125"
                     /old_locus_tag="V3O01_20130"
     CDS             complement(498..854)
                     /locus_tag="V3O01_RS20125"
                     /old_locus_tag="V3O01_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000269904.1"
                     /translation="MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSNKKRFYTEMLAIADEQ
                     YALHLSTLGDQSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1420..1719)
                     /locus_tag="V3O01_RS20130"
                     /old_locus_tag="V3O01_20135"
     CDS             complement(1420..1719)
                     /locus_tag="V3O01_RS20130"
                     /old_locus_tag="V3O01_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            complement(1929..2447)
                     /locus_tag="V3O01_RS20135"
                     /old_locus_tag="V3O01_20140"
     CDS             complement(1929..2447)
                     /locus_tag="V3O01_RS20135"
                     /old_locus_tag="V3O01_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000839337.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPVQKQSVKETVKVDSPTSAEVLLLRQQIDLLKNQLDDAKNRESFYQNQIETMQRLL
                     EAPKTNTTQSDDPGSTQAESTVEEKEDVIAMPPKESEVIENKRIPVPEHIEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2525..3460)
                     /gene="repM"
                     /locus_tag="V3O01_RS20140"
                     /old_locus_tag="V3O01_20145"
     CDS             complement(2525..3460)
                     /gene="repM"
                     /locus_tag="V3O01_RS20140"
                     /old_locus_tag="V3O01_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001292329.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLREASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YESQSGIIFLRFTQDIVPLITRLEENFTKYELQQVSRLSSSYAIRLYELLIQWRSAGKT
                     PLFDLSIFRQQLGVKPHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFTFKQKKMSNLPIKNKRDPDTIDIFSKMTDAQRHLFSHKLSELPEMGKYSHGTESYPQ
                     FAVRIAEMLQNPEKFKELYPYLQKVGFKAA"
                     /name="repM"
     gene            complement(4057..5226)
                     /locus_tag="V3O01_RS20145"
                     /old_locus_tag="V3O01_20150"
     CDS             complement(4057..5226)
                     /locus_tag="V3O01_RS20145"
                     /old_locus_tag="V3O01_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            5531..6292
                     /locus_tag="V3O01_RS20150"
                     /old_locus_tag="V3O01_20155"
     CDS             5531..6292
                     /locus_tag="V3O01_RS20150"
                     /old_locus_tag="V3O01_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            6409..6708
                     /locus_tag="V3O01_RS20155"
                     /old_locus_tag="V3O01_20160"
     CDS             6409..6708
                     /locus_tag="V3O01_RS20155"
                     /old_locus_tag="V3O01_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000358348.1"
                     /translation="MDKKIEEKTKDINEKIQKLDLAIQELIETTDNFFINDKYYGIECA
                     FILDHALNIKMHFFNKYPAFAPPHLRGVENYDRIIHIRENNPEDKLEQISPLKD"
                     /name="hypothetical protein"
     gene            6976..7263
                     /locus_tag="V3O01_RS20160"
                     /old_locus_tag="V3O01_20165"
     CDS             6976..7263
                     /locus_tag="V3O01_RS20160"
                     /old_locus_tag="V3O01_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438827.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            7250..7564
                     /locus_tag="V3O01_RS20165"
                     /old_locus_tag="V3O01_20170"
     CDS             7250..7564
                     /locus_tag="V3O01_RS20165"
                     /old_locus_tag="V3O01_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            7692..10103
                     /gene="znuD2"
                     /locus_tag="V3O01_RS20170"
                     /old_locus_tag="V3O01_20175"
     CDS             7692..10103
                     /gene="znuD2"
                     /locus_tag="V3O01_RS20170"
                     /old_locus_tag="V3O01_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            10408..10869
                     /locus_tag="V3O01_RS20175"
                     /old_locus_tag="V3O01_20180"
     CDS             10408..10869
                     /locus_tag="V3O01_RS20175"
                     /old_locus_tag="V3O01_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     Typing          complement(2526..3460)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4061..5148)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaagctatt tcgtaatatt ttatccaata agcattgtca aaaatatcaa tgtaatagct
       61 tggcctctta caatgtgaaa aatattcaca gcataaatct ctaataattt caatttcttc
      121 taggctatcg gcagcattga ctaaaaatat aaacttgtct aagttaacaa gtaatttagt
      181 actatttttc aaaggtttat tattagacat tgaaccctat gtgttttcct gttggaagct
      241 ctacatcaat acgaagtttt ccacccattg cttcaacata ctttttcatc gttgaaattt
      301 taagatcatg accacgattt tcgattgctg ataatgacgg ctgtttaatt ccaagggttg
      361 cagctagttc tttctgggaa atttctaact cttctcggat aaggtgaagc tgactttcta
      421 aaagtaattc atcagccatt ttttgaatac gagcttgact ctctggagag cgactagcca
      481 ataattcttg taagctctta gccattagat tgatctccta aagttgagag atgaagtgcg
      541 tattgctcat ctgcaattgc tagcatctct gtataaaaac gctttttatt actcttatca
      601 ccaatacaca gcacaatcgc ttgtcttagg ggatcgaatg caaaaaatgc ccttaacggt
      661 ttaccacgat gctgaacacg tagttccttc atattggtaa atttagaatc gtagacggta
      721 tctactaaag gacgacctaa actaggccct tgctgttgta gaacaaccaa tgcagctaag
      781 actttctctt gtgttgcttc atcttgctgt tcaagccatt cattaaacag atccgttgta
      841 atgactgtcc acatattaca atcacaatat agattacagt ctatatatta aatctgattg
      901 aatgactttg caaatggcaa taaaaaatcc cgacgtcatg tcgggatttt tcggtatgga
      961 gcgcataggt agggctgact acctgatggt catggtgcct gactgattat tattgttgtt
     1021 ttaagtcatt gaacatcctg tccatataga tcgatcataa gagcttcatc gggggataga
     1081 tacagacaat ttcgtgaaac gctgtaagcc aaaacgtaca aaaaataatc aaaatttatc
     1141 ccagtggtta tccacagaaa tagtggattc ttttgggtaa aaaagcttat aaactctatg
     1201 gaattgctat ttttgattat tttttaaaca attttattgg caagaagtat caaaattgag
     1261 cataaaaaaa ccgacttgtt tctaagtcgg ttgaacgagg tcgttccaga gggtatgact
     1321 caatgtaagc tactgatctt aaagtaatct taaagatcca tttcgcataa tatccattat
     1381 gttaaatagt aggggactct aagtacaaga ttagaaaatt tagtctttaa gaggagaaat
     1441 ttgttctagc ttatcttcag gattattttc tctaatatgt ataattcgat cataattttc
     1501 tactcctcgt aagtgaggtg gtgcaaatgc tggatacttg ttgaaaaagt gcattttaat
     1561 atttagtgca tggtctaaaa taaaagcaca ttcaattcca tagtacttat cattaataaa
     1621 aaaattgtct gtagtttcta ttagttcttg aatagctaaa tccaattttt gaattttttc
     1681 attaatatct ttagttttct cttctatctt tttgtccatt ggtatcacct atagaattaa
     1741 gtttccctaa aattaacata atacacctta tacgaaattc tttgtaaaaa gcattttaaa
     1801 acaatgtctt atggatgtaa aaacagccta agcagtcacg ctcaggctgt tttagtgatt
     1861 ttttatgttt cacgatcaat tattgatcat tgttccacgg ttttgattag tctgattaaa
     1921 tagtcctatc atccataggg cttgaaaaaa cggctccaga agcccctttt ttctggttct
     1981 ggctcaatat gttcagggac tggaatacgc ttattctcga taacctctga ctccttagga
     2041 ggcatagcta tgacgtcctc tttttcttct actgtggatt ctgcctgtgt tgatccagga
     2101 tcatcagatt gtgttgtatt ggtttttgga gcttccaata ggcgttgcat agtttcaatt
     2161 tgattttgat agaagctttc tctatttttt gcatcatcta gctgattttt tagtaggtct
     2221 atctgctgtc taaggagtaa aacttctgcc gaagttggac tgtctacttt tacagtttct
     2281 tttacactct gtttttgtac tggctcacca aatacccgga tcgcttctgc aagatcaatc
     2341 ttattgtcag aatttttact taaaatacct ttctttatat tgttgtaaat ggtttgtcga
     2401 ttaatattgt aaagcttaga aagctcaaga actgtaaggc ttttcatgtg taaatctctg
     2461 cctaatcttg taaaaaatta gtgtctatta gactgtctaa tagacaaatc agcaagtcta
     2521 tcttctatgc agctttgaag cctacttttt ggagataagg atagagttct ttaaactttt
     2581 ctgggttttg aagcatttct gcaatgcgta cagcgaattg cggatagctt tctgtaccat
     2641 gagaatactt tcccatttca ggaagttctg acagtttgtg ggaaaacaga tggcgttgag
     2701 catctgtcat ttttgagaaa atatctatag tgtctgggtc acgcttattt tttattggca
     2761 gattactcat ttttttctgt ttgaaagtga atgaaaaacc tgaaatagaa cgcccttttt
     2821 tatgttgctc atatttagct gttatatcgg ttaactcatt tacctgctta agagcaaaat
     2881 ctaaaacata tgttttaaag ttactcattg ttttgtattg atgaggtttg acaccaagtt
     2941 gttgtctaaa gatagataga tcaaaaagtg gcgtttttcc ggcagatctc cattgaatta
     3001 atagctcgta tagccgaata gcatatgagc tacttaacct agaaacctgc tgcaattcat
     3061 atttagtgaa attttcttca agtcgagtta tgagtggaac gatatcttgg gtgaatcgta
     3121 ggaaaataat tcctgattga gattcatacc caattttgtc gacccaacga caatgaaagc
     3181 tacgatcctt acctgtctta ggattaatgt catggtatgt gacatagcga tcaaataagc
     3241 ttttagatgc ctctctgagt acggtataag cggtatgttt ctcgacatta aaagtattta
     3301 tataactact tgcatgtact tctaaaagac tattttcagt tatcccatgt ccagtttctc
     3361 gagcttcagc aatggctaat aggatcagtc tttgttcaac tgtatctaag gtataacttg
     3421 cttgaattag agcattatcc tttacgatta attcactcat ttctaataat aaatttataa
     3481 ttaaactagg ttgtacaata gactatttca attgagttgt aaattaactt aattatgggt
     3541 cgataaacct agctttaagg tcgataaacc tagttttatg ggtcgataaa cctagtttta
     3601 tgggtcgata aacctagttt tatgggtcga taaacctagt tttattaact atgaaactat
     3661 tataaataaa ggtttacagc ttatttaaaa gcatttaaaa gattaaaaat aattaaaagc
     3721 ccaatgaatg aaaaattgcc ctatgttttc gctacgctca aactctattg aacttcgctt
     3781 acgctcagtt cgaaggggca atttttgggt taatctcgtg ataacttcaa aaaagcaaaa
     3841 gcaactcgga attcgcttcg ctcataagtt ttttatactc gcttcgctcg acctagatgc
     3901 aaattaaatc tgttcgcacc ctccgggatg ctctgtatct cagtctcagc agcaaagggg
     3961 agtttgtaat cagggaaaaa gcatggcaaa aattttaatt gtgtctgttc gctcgtagac
     4021 actcgctctg taaactttgg ataaaaccaa ctgagcttaa aaaccagggc caccgtaatc
     4081 ataatcatca gctttaggtt gtactggttc tgattgagtg cgtttgggag gcgtattttg
     4141 tatcggtaat acgtattcag gaagtgaatg cacctcgaac tcaaaacatg gggtactgtt
     4201 acgtcttgag tcttctagtt tttcattcat ttcgtagatg gaccaagctt cttgtttaag
     4261 tatagggcct tggtttttga taatttcatt caaatcattt tttagtttgt ccttatttgt
     4321 tgcattcttg attaagtaat tcaggtaata catgacttcc aatgatgaaa atgggttgtt
     4381 ttcagcacta taaaaatatt catgttgttg atctaattgc ttttggcgtt cttcgagttc
     4441 tcgttcttgc tggttgtgtt ctctgatttt ttggatttgg gcgagatatt ccgctgtcgc
     4501 attttgttgt ccaacgccta gttttggctc tggaaggtat agcgtgctgt ggatctcgtt
     4561 atatttttca tagctgtctg ctgaaacctg atttttgatg gtgatgtctt ggccatccag
     4621 tgtgtaggtg acggtcgggc agtgctgtgc aagggcatca ttcaaatgat tttcccattg
     4681 ttttcggata tagatcaaaa aagtggaata gtcttcatgg ccatcttgaa gttggatttt
     4741 tttggcaccg cctttggctg gatcttgagg gttatagcgt ttgaagtact gttctgcgct
     4801 gcgttcaatg ccgtcatccg cttttagcga gaacatcaga tgacaatgag gctgatcttc
     4861 gccactgata cgtgatttta cgttgtgtat ggcgtatgag tgcggatatt gctgtggaac
     4921 tatatgtttg tctaaaaacg tctctatgag cgttttacgc tgttctgacg tgaactcatt
     4981 cggtagggca atttcatact ccatgtaggc cttggcattg gctcgttcat acgtatctgc
     5041 tgcctgccaa aaatcaatgg gatcttttac aaaggcaggc atacatcgac caggttcaat
     5101 atgttctatg gtctcattgc tggttttgcg gatgccttca tactgcatca gccgggaaat
     5161 gtagagataa tggccggtcg catagccctt atttttgctt ttgacgctga agtggtagct
     5221 ggccatgttc acctctgctg ggttgtgggt tcggttttta tgcagtgaaa cggaatgaaa
     5281 accgcccgga tttgaaatgc ctgtatggct gaaaaatccg gttgaagggg gtcaaggggg
     5341 aagcgcccca ttgcgtacta gtttctccga tgaggagaaa ctgtcaagtg cgaattattt
     5401 aatataaaaa taaaatttct tgaaaagaat ttttattttt atattaaata attattttca
     5461 gactgccctc tttaaatatg gtaaaaccat atttaaagag gttgcagttc tgaaaatttg
     5521 gcggcagatg atggaaatgg aaaaagcaaa aaagcgtggt cgacctgctc agttgttaca
     5581 gattgctgag ttacatgcat ttgtagagtt tttagaacag caagaacagc tttctgatct
     5641 tcaatctcaa gtgttaaagg ctttaaatag tgttgattgt aattttgagg ggctaactca
     5701 gacagatcag gttctggtca aagaggcttt aaaaccttat cgtgaacatc tgaagttgaa
     5761 attgctgttt gaagagttga ataatctgcc tcttaaaacc gagtatgaac agaagttttt
     5821 agatctgtat gaactttttc aaaagaatgc actagatcag atggaactaa atattttaaa
     5881 aaccttggcg acacggtatc tgaattttaa agcgcaaaag cttgaataca gtgatttaga
     5941 attgtatttg agtcagctac agaagaagga tgctgggaaa aaacgcaaag ctgaaaatca
     6001 acgtaaattt gaattaggtg gtgctgttct tgtcgcattt aagaaactca atattgatat
     6061 tagcaatgat actcctcaac agattaccaa tcggattgtg aatacgacaa agtttcataa
     6121 tgaagtacgt aaatctttaa tttttaaaga tgttaagaca tatgaaaatg aatattttaa
     6181 agcaaataaa ctttttattc aggtgttaga aggattacat acctggcaaa agggcggaga
     6241 attattgtca gtaattgaaa ttaaaaaggc cttagaaaaa ggcgaagagt agtttattta
     6301 gcagggattt tagagaagtt ggacatttct attttgggct tacattggaa tttcgcataa
     6361 ggtgtattat gttaatttta gggaaactta attctatagg tgataccaat ggacaaaaag
     6421 atagaagaga aaactaaaga tattaatgaa aaaattcaaa aattggattt agctattcaa
     6481 gaactaatag aaactacaga caattttttt attaatgata agtactatgg aattgaatgt
     6541 gcttttattt tagaccatgc actaaatatt aaaatgcact ttttcaacaa gtatccagca
     6601 tttgcaccac ctcacttacg aggagtagaa aattatgatc gaattataca tattagagaa
     6661 aataatcctg aagataagct agaacaaatt tctcctctta aagactaaat tttctaatct
     6721 tgtacttaga gtcccctact atttaacata atggatatta tacgaagtac acttgtatat
     6781 taatttaaat atcaactact taataaaaat ataaaaatcc aaaaatgccc aaaaagccga
     6841 ctttgaaaca agtcggcttt tttatgatcg attttgatag tttttgccaa taaatctgac
     6901 taaaaattac gcttctcaac ttgagtgata caatttcgtg atacattatt gtatatacaa
     6961 atttgaatat tttttatgga acagtatttt gaatgggatg aggctaagaa tcgaaagaat
     7021 cagaaaaagc atgatatctc tttcgaaact gcaagccttg tttttgaaga tccattacgg
     7081 atctcaatcc aagacagata tactaatggt gaagaacgtt ggcaaaccat tggacgagtt
     7141 aaaggcgtac taatgttatt ggtggctcac accatttttg atgaagatga ctgtgaaatc
     7201 atccgaatca ttagtgcaag gcaagtcact aaagcggagc gaaacaaata tgagcatggt
     7261 tagatactca cacaaagagc tgaatgagaa atttggtgag aagcaagatg ctgaaattca
     7321 acgcttgctt gctaaaggca cggttcctga tgatcagcta gatctatcag atattcctga
     7381 aattacagac tggagcaatg ctgtacgcca aaatcaattt taccgcccag taaagcaaca
     7441 aacttcaatt cggttagatg ctgatgtact tgcttggttc aaagcacaag gcaaaggtta
     7501 tcaaacacgt atgaatgaaa ttttacgaga tgctatgcta aaagaattaa aaaatcatca
     7561 ataagaatgg gagcttaggc tcccattttt gacttcattt ttagggggga ttaccattca
     7621 aaaatttaaa tagtagattt tataaatttt taagtgttat attataatat aacattaaat
     7681 ttggttgttt tatgctaaat aaaagtaaac tatttttagc tttaattact ttaggggcct
     7741 cgaaaatttt attggcagct gaagggcctg ttactacctt aaatacaata gtattaactg
     7801 cacaatctga tgagttaggg tctgagctgt taggcaaaag tctaaatgtt agtaatcagt
     7861 ttattgatac tagtaaactt aaacagcgtt cgacaactct aggggatgcc ttgggtacgg
     7921 aattaggaat tcattctaat caatatggag gtggagcatc tactcctatt attcgtgggc
     7981 aggaaggaaa acgtattaaa gttttacaga ataatgctga cgtgttggat atgtctaata
     8041 tgtctccaga tcacgctgtc acagttgaac caagtttagc taagtctatt gaaattatac
     8101 gtggtgcaag tacccttttg tatagttcaa atagtgcggc aggggtagtc aatgtcattg
     8161 actataaaat tccaacccaa atgcctcaag acggattaga gggtaatact acattacgtt
     8221 ttaatactgg cagtaatgag aagttgacca cagcaggtgt aacagttggc ttaagcccac
     8281 atgtagcttt acgggcagaa gggttatatc gtaatgctgg taactataaa actcctcatt
     8341 accagtctag ctcttataat tctttagaag atcttgaaaa ccaaaacagt atttataaaa
     8401 atttaaaata tttaccagaa agctgggcag agtcgagagt aggtacgctt ggattatcct
     8461 ggatagacga taatacttat ttaggagttt cttatacaca tcgtcatgat gaatatggtt
     8521 taccagccca tagccattta tatgaaggtt gtggtgcctc tgcaatcggt atcgattcac
     8581 gaattagtgg attaaaaaat tatcttttat attatcctca attgatggat gagcaagata
     8641 ttaattacat aaatccacgt cctgattgtc atcagcataa tcatattcat gaaactaatt
     8701 tctctcataa tgctccttat attgatttaa atactcgacg ttatgatgtg cgtggagaat
     8761 tcactcaacc atttaccggt attgacaaaa ttcgtacctc attgagttat atagattatt
     8821 ttcacaatga actcgaggga gataagatta ctaacttttt taaaaataca ggaaaagtag
     8881 gccgcataga gttatcacat cagccattag gggaattaac cggtatcttg gggctacaat
     8941 atttggaaca ggataatagt gctttatcac cagtccattc tcaagaaggc cataccacct
     9001 atctagataa tcagcagcta ttaaatcgaa atgtcactaa aaactttagt gtatttggcc
     9061 tagaaaaata taactggaac gactttactt tcgaactagg tgctcgtata gaaaagcaga
     9121 aagttagtat ggattatgat atagaaaaaa ttaaagattc aatgaaacct tggcctaata
     9181 aatataacag tccttatgta gaaaaaaata ataaaatcag ggctcaaaat ttaaaatcta
     9241 ttttagaagc tgttcaacct aacaaagaaa cagctttttc ttatgcaggt acagtccact
     9301 ggagatttgc tccaaattat atcttatcat taacaggtac acatcaagaa agattaccta
     9361 atgcacaaga aatgtatact catggtatgc atctcgctac taattctttt gaaataggta
     9421 accgctttct aagaaaagag aaatccaata atttagaaat cagtctggcc tataaggatg
     9481 atttacttga ttatcaaatc agtacttact attatgattt tgataactat atatatttac
     9541 agacactcaa tgaagtttta ggaactacaa aagttagaga tcagcataca ctacgtatta
     9601 atcattatag ccagtctgct gctaattttt atggattaga gggcaacata gggtatcaat
     9661 ttaattctgt ttatcacggc tctctttttg gagattatgt gaaaggccgt ttaacaaatt
     9721 tacctgatgc tgtaattgca tacgatatct ggaatagaga accgacttta gctccccaaa
     9781 aggatcgtta cactcctaga ttacctcctg ctcgtttggg tacacgacta aaagctgatt
     9841 ttgatgaatc gcttaaaggt gaaatagaat attatcgagt ctttaaacaa gataatattt
     9901 ctaaatttga gcaggttaca tcaggttata atatgcttaa tatgacttta gcatataaaa
     9961 ataagctatc tcacacagaa tatgatttat tctttaaagc gaataattta cttgatcaga
    10021 aagtctatgc ccatgaaaca tttttgccat atattcccca aattggaaga aattttagtt
    10081 taggcttaaa tcttaatttc tagataagat actgtaatct tctaagaaaa tttcttagaa
    10141 gatttttctt ataaaatgat tagattaata attaaggtaa gcgtccgctg aatcccatac
    10201 ctatttttta attcgattta ggtttccagc agtgtggcag taactcttca atctgggtca
    10261 ctttatgtgt cggcagcctt ttcagcactg aatagccacc gattttgtag acacctttta
    10321 gctagataaa aagcaatttt tttgatcttt aaagaatgag gatataagct gtttaaaact
    10381 taattctaat tggttaatag gtgaaaaatg aaattagcag aagctctctt acttcgaagt
    10441 gatcaacaaa aaaaaattat ttcactaaaa caacgtatca atgcaaatgt attggtacaa
    10501 gatggtgatc aaccatctga agatccaaat gaactactaa aacaagtatt ttctttaatt
    10561 caagaatttc aaaaattaag ttatgcaatt catgaaacta atgctttaac aaaattaaat
    10621 gatggccgtt ctctacttgc tttattgact ttgcgtgatg aatttgtaga acagcacaaa
    10681 accctaactg cagcaataag taatacatct cgtgaatctg atcgttatag tacgcgggaa
    10741 ataaagtggc ataaagttat tccagtttca tcattacaaa aacaagctga tgatattagt
    10801 cttaaattac gtgatttaaa cgtgttaatt cagtcgacta attggaaaat tgatttgatt
    10861 gaagcctaat agtcctttag atataatcaa gttgaacata ctgggcgagt acaagatccc
    10921 ttacttccac aacataggat tgtaaataaa gtgtagtggc ttgcaaagca agtggataag
    10981 cttaaaattt taagtacaaa tcaatccatc acttggcata actcaaatct ttgtgcaaac
    11041 aaagttacac gttacttata actaccatgt actgacagta tgttcacctc aattactaaa
    11101 aataaagaaa tgagcaagca tcagctcaac tacatttcta tttttaattc atttcggcat
    11161 ttaacataaa atctcttatg cgaatctaga agga
//
