LOCUS       NZ_CP144237            12747 bp    DNA     linear   CON 05-APR-2024
DEFINITION  Klebsiella pneumoniae strain CSMH-KP2 plasmid pColRNA_CSMHKP2,
            complete sequence.
ACCESSION   NZ_CP144237
VERSION     NZ_CP144237.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39600918
            Assembly: GCF_036606455.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Lai,Y.-C. and Lee,Y.-L.
  TITLE     In vivo conversion of KPC2 to KPC33 in K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   Teng,R.-H., Tang,H.-L. and Chen,B.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Microbiology and Immunology, Chung Shan
            Medical University, Jianguo North Road, Taichung 402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036606455.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:44:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,979
            CDSs (total)                      :: 5,856
            Genes (coding)                    :: 5,684
            CDSs (with protein)               :: 5,684
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 56 of 172
            Pseudo Genes (incomplete)         :: 117 of 172
            Pseudo Genes (internal stop)      :: 19 of 172
            Pseudo Genes (multiple problems)  :: 17 of 172
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144237.1.
            Bacteria and source DNA available from YICHYI LAI.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CSMH-KP2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNA_CSMHKP2"
                     /geo_loc_name="Taiwan:Taichung"
                     /lat_lon="23.3 N 120.9 E"
                     /collection_date="2023"
                     /collected_by="Chung Shan Medical University Hospital"
     gene            complement(99..830)
                     /gene="mobC"
                     /locus_tag="V1F70_RS29825"
                     /old_locus_tag="V1F70_29825"
     CDS             complement(99..830)
                     /gene="mobC"
                     /locus_tag="V1F70_RS29825"
                     /old_locus_tag="V1F70_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(830..2794)
                     /locus_tag="V1F70_RS29830"
                     /old_locus_tag="V1F70_29830"
     CDS             complement(830..2794)
                     /locus_tag="V1F70_RS29830"
                     /old_locus_tag="V1F70_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4278..4397)
                     /locus_tag="V1F70_RS29835"
                     /old_locus_tag="V1F70_29835"
     CDS             complement(4278..4397)
                     /locus_tag="V1F70_RS29835"
                     /old_locus_tag="V1F70_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_194315302.1"
                     /translation="MGVITGFLLAACQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4398..5095)
                     /locus_tag="V1F70_RS29840"
                     /old_locus_tag="V1F70_29840"
     CDS             complement(join(4398..4846,4846..5095))
                     /locus_tag="V1F70_RS29840"
                     /old_locus_tag="V1F70_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(5289..5546)
                     /locus_tag="V1F70_RS29845"
                     /old_locus_tag="V1F70_29845"
     CDS             complement(5289..5546)
                     /locus_tag="V1F70_RS29845"
                     /old_locus_tag="V1F70_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5556..7241)
                     /locus_tag="V1F70_RS29850"
                     /old_locus_tag="V1F70_29850"
     CDS             complement(5556..7241)
                     /locus_tag="V1F70_RS29850"
                     /old_locus_tag="V1F70_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(7346..8833)
                     /gene="ltrA"
                     /locus_tag="V1F70_RS29855"
                     /old_locus_tag="V1F70_29855"
     CDS             complement(7346..8833)
                     /gene="ltrA"
                     /locus_tag="V1F70_RS29855"
                     /old_locus_tag="V1F70_29855"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9478..9723
                     /locus_tag="V1F70_RS29860"
                     /old_locus_tag="V1F70_29860"
     CDS             9478..9723
                     /locus_tag="V1F70_RS29860"
                     /old_locus_tag="V1F70_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9997..10368
                     /gene="tolA"
                     /locus_tag="V1F70_RS29865"
                     /old_locus_tag="V1F70_29865"
     CDS             9997..10368
                     /gene="tolA"
                     /locus_tag="V1F70_RS29865"
                     /old_locus_tag="V1F70_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            10365..10730
                     /locus_tag="V1F70_RS29870"
                     /old_locus_tag="V1F70_29870"
     CDS             10365..10730
                     /locus_tag="V1F70_RS29870"
                     /old_locus_tag="V1F70_29870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            10905..11183
                     /locus_tag="V1F70_RS29875"
                     /old_locus_tag="V1F70_29875"
     CDS             10905..11183
                     /locus_tag="V1F70_RS29875"
                     /old_locus_tag="V1F70_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            11171..11485
                     /locus_tag="V1F70_RS29880"
                     /old_locus_tag="V1F70_29880"
     CDS             11171..11485
                     /locus_tag="V1F70_RS29880"
                     /old_locus_tag="V1F70_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            11649..12077
                     /locus_tag="V1F70_RS29885"
                     /old_locus_tag="V1F70_29885"
     CDS             11649..12077
                     /locus_tag="V1F70_RS29885"
                     /old_locus_tag="V1F70_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            12103..12282
                     /locus_tag="V1F70_RS29890"
                     /old_locus_tag="V1F70_29890"
     CDS             12103..12282
                     /locus_tag="V1F70_RS29890"
                     /old_locus_tag="V1F70_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(12309..>12747)
                     /locus_tag="V1F70_RS29895"
                     /old_locus_tag="V1F70_29895"
     CDS             complement(12309..>12747)
                     /locus_tag="V1F70_RS29895"
                     /old_locus_tag="V1F70_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338459813.1"
                     /translation="VLAWQTGAYVFCFVDRAQHLAMMKSIGMYDERLNSDEKATYFFTG
                     ENPQWERDKPTGMKLCDYLYRKVERFAIENGVQVVDAGKCSPEQINAMIEVVSERHGES
                     AVNEKLKAELMREYNSRYFNVPVPELPEDKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             556..12241
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3597..4175
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(103..830)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 accatgtctt ccagatgctc gctggttggc ataatgcagc gctcgccgtc tttgtcttcc
       61 acgtcctcaa taaataccat gtacagcgcc ataatccctc actccgttgt cataaattta
      121 aagcacgcga acagcctctc ccggtccagc tccaccgttt tgcgggcctc gccggtctgt
      181 ggattcacga tttcatccac cgccagaatc gcccgctgta cccggacaag cgtcggccgg
      241 tcagggcaga tgtaccagat ttcatcgatg ccgttcgccc ggcggttgaa cagatacccg
      301 gacaccactt cctgataccg cctgcggctc ttgacggtgc gctctacctc aaatgccacc
      361 gttctgccgt ccagctccgc cagcgcgtcc ggcactttca tccctttcag ggccatacgg
      421 tgcaggtacc gccagccggt ggccccccgg ccctccatgg agaggcgcag ggactgcacg
      481 gccagccggt gcggcagctg cgccgccgac acccttgacg gctgaaacgg aataacgtcc
      541 gtcagcggct cgtcctcgtc gaacgagaac gccaccccgt gcggcgtcag cccccacacg
      601 ctgacctgac ggccgtcaac cgggtatttc gcccggacca gcagcccttt cgccaccaga
      661 ctgtccagga agcggtaaac cggctgtacc gccgcaatcc ccagccgtcg cgcaaccagc
      721 tccgccgtgg aacacagctc ttcccgcagc cagcgcaggg tcagccgtgt tttctccgcg
      781 ttccgcgcct gccgcttcgc gtggctgacg ttgtactgct ccagtgtcat cagtaaagcc
      841 ccgcaaaatc atcatccgct tttggtccgc cgccgtcggc cggaggcagg tactccgcca
      901 gcgggtccgt tttcgccagc accgggaagg ttttcagcga aatttcccgg cgctcaaccc
      961 gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc gcgaacccgt
     1021 caggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc tggatgaagc
     1081 tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg cgctccacgt
     1141 ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg ctggccgcgc
     1201 gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa tcagcgggca
     1261 gatcctgccc gcactgaccg aagtcgccca gcgactggtg cgcgagcagc aggttgcagt
     1321 cccggctgcg cagcgtcccc agcgcgttca gcacgtactt cgacagcaga tatttaatct
     1381 catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca atgatgattt
     1441 gcgcgcagcg ggcaaacagc atcttctgca cccggatcac cgcctcatcg tccatcgagc
     1501 cgatgacgta caggcagccg ccgccgttga ttatccccgc cacatcgata ccgctgtccg
     1561 tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc ttcacgtcct
     1621 ttttcagggc ctccggcaac gcgtacgccg ccgccagcac ctgctgaatg tttgccccct
     1681 gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg tagtggtccg
     1741 ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc agggcgtact
     1801 ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga tccagcaggg
     1861 tgaagtccgg gcagtgcgcc cggaagacgt gcggcgccca cgcgtcccct ttggggtcga
     1921 agtacaccac agcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg gtggccatcg
     1981 cgctcttgcc gctcccgggc aggccgagga tctgaatatt ggtcttgcgc caggtatcac
     2041 gagggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc tgctgtttcc
     2101 gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc ccgaaatgca
     2161 ggtggcggac gtctgtcatt tctccggtcc gctggctgct gcgccggctc tcctgattca
     2221 gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc atcaagcagc aggaacgcca
     2281 gcagccgcgc cagaaggacg ccggcgacaa gccctccggc acaccaggct atcccgaaca
     2341 ggttagccgg ctgcagcagg taataatctg cgttcgcata gaggtccggg cgcaggacgg
     2401 tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg gccagaccgg
     2461 cagcgccggt cagccagtac gcgtagttcc agacatgtcg ccgtgccctg accatccgcc
     2521 cgggtgaatc cagcgaggcc agcaccgaaa atggcacagt ccggttatta tcgcgcttat
     2581 agcgatcggc atactcccgc cgcgccttat ggctcagccc cacaaaacgc attcggccgg
     2641 cccagagttc agccagaccg aaaaacaggc cgataaccag aaaaaaaccg gccagactgt
     2701 aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc tccgggatat
     2761 gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc agacgacggg
     2821 tttaccggcg tgatgcgcag cccccgctgc acaagccgcg gacatgcccc aaggcatggc
     2881 ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc agcctctttc
     2941 cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca gcgtgtgaga
     3001 ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc agagttcgcc
     3061 atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg tcaggtgcgt
     3121 ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc gggtgaattt
     3181 ctggcgtggc cggcgtccag ccggacagag ccagaaagag gggaaaagac cgtgcgtcgg
     3241 tggcgtccag ccagcgtaag caaggtcttt tcccctgaag tcagccacgc cccagcgggg
     3301 atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac gtaaaatgtt
     3361 cagcgaaaaa ccgacatggt tcacctatcc tgataattga tcgtcaggga atagaaagac
     3421 ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca ggtgccgggg
     3481 aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc
     3541 tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag
     3601 gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt
     3661 tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca accatgaatc
     3721 tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg gtaggtcgct
     3781 ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc gccgttctcc
     3841 cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc tcggtgtagg
     3901 tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac tgctgcgcct
     3961 tatccggtaa ctgttcactt gagtccaacc cgggaaagca cgcaaaacgc cactggcagc
     4021 agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg
     4081 aagtgtgcgc caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa
     4141 agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc
     4201 ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga
     4261 agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt
     4321 gccaccgttc cgccctgaac atcccggata taatttgcct gacatgcggc cagaagaaag
     4381 ccggtaatga ctccaattta ctgatagtgt tttatgttca gatagtgccc gatgactttg
     4441 tcatgcagct ccaccgattt tgagaacgac agggacttcc tgcccagcct tgccagatgc
     4501 tgtctcagat tcaggttgtg tcgctcaatt cgctgcgtgt aacgcttact gatgacgtgc
     4561 agttttccct tcagacgtga ctcatacatc ggccagccgt ccgtcatcca taccaccacg
     4621 tcaaaaactg acagcaggct cagaagacgc tccagtgtgg ccatagtgcg ttcaccgaaa
     4681 acgtgggcta ccaccgccct gcgtatcctg tcatacgcgt aaaacagcca gcgctgacgt
     4741 gattttgcac cgacgtagcc ccattgctcg tccatctccg cacagacaat gacatcactg
     4801 cccggctgta tccgggaggt tactgactgc ggcctgagtt ttttaaatgg cgcaaaatgg
     4861 tgttgaggcc cacgcccatt agtcgtgcgg tggcacggca accgacgccg ttcatagcca
     4921 tatcaatgat tttctggtgt gtaccgggct gagaagcggc ataagtgaag gtgagctgcc
     4981 atgttttacg gcagtgagag cagagataac gttgatgccc ggcagtactt ttgccattac
     5041 gcatcacgcc ttcagtagcg gaacaggagg gacagcagac ggagactgaa gccacgggag
     5101 cacctcaaaa acaccattat acactaaatc agcaagttgg tagcatcacc aagaaagcca
     5161 gcaacaatca atataaaaag aaaaatagtc tttacctttt tcatatactt tccctgtatt
     5221 taaagactcc ggcattcatc cctgaacacc ggaagtcagt atttcaaatc atccttgata
     5281 attttttgtt accagttacc gtcccgataa tcaaaagcta tataacaatt atccacatca
     5341 ggttttattt gatttttaaa aaatggctgt agtaaaggaa cccattcttt cttcaaatca
     5401 aactcaccat tattaatatt atcctttaaa ggcattccca agctttcaat gacagaacca
     5461 tcgtcaccaa agtctttaga atactcttcc cctttaaact cttcagtttt cttatcaaac
     5521 catgtcaaac gtaatttaag ccccatattt acctctcaaa gatatttttt aatgttccgt
     5581 tttggatctg gccctttaac ctgcttaccc gttttgggat caaatgcgcc taggtgttcg
     5641 ccatcactgg cacgataccc ttcaagctca ccgtgctggg aatcccattc ataaatttta
     5701 cgttttttat cgccatacca tcgggcccgc ttaccaccac caccttgttt aggtgtttta
     5761 ggatcacctc gctttaactc tccaagtccc tttatgtctt ctgttttagg tactggatga
     5821 taatcatggc cgtagtcttt agtccccttg cgaggcttgt tcttttcctc atttaactta
     5881 ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc cccggtaagt
     5941 gcagccttat ctctggataa ggattcattc attgcgttta aagatgcctg agccgctttt
     6001 aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc ctgccacccg
     6061 gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc ctctgcctct
     6121 tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggagcgc cttctctgtc
     6181 gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt cggcatccag ctcagctttc
     6241 gctttatcat agtctctttt cagcccctct tccggatgag cggcgtccca tgcctgctgg
     6301 cgacgctttt cttcctcctg acgctgtttt acctgtgccg gggtaagaac atctgtcacc
     6361 gacacataaa ccggcttctg tccggtctct ttcgggaaac gaataacggc ctcacgggag
     6421 ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt tttcagcaac aatgcctttt
     6481 ggcggggctt tcgctgtcgg aacacctttc ggtacgctca cctgcagaga cgggagaccc
     6541 ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac cacagggaca
     6601 ctcattggcc gacctgcgac aaccgcaata tgctgccgct catccttcac cacatccaca
     6661 acccgttgtc tgacacgaac cgtcgcctgg tccagtggta aagtacttgc cggagtctcc
     6721 gtcaccgcat cggccggtaa tgacgtcata atttttgaca tcattttcgg atcatctttt
     6781 gcaatctcag aaggcagcac accgtagatc gcaatccccc acagaccaaa cttaaacggc
     6841 cccttcaggg cagccagcac atcagcaacg gcggccgaca acgcatcgcc ggaaactgat
     6901 aaagccagtc cttcagcccc cggagtagcc agggcaggca gaccgaaggc catcgcagtg
     6961 gccgaagact gtccgccacc agagctaccg ctatgattgc cgctgttacc accattacca
     7021 ccgggtttac tcccgccgtt accaaactcc cccggattat agttatgaat atctccattc
     7081 ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc acctgatgaa
     7141 gggccacctt taccagacgt accgttcaca ttcccactgg cctgaccacc attatgtccc
     7201 agacctgaat tacccggacc tcgaccgtct ccaccactca taccgttttc ctctttgaca
     7261 tttaaaacaa taaattaaag ccaacatgtc gcggtaggga tacccgttac cggatacccc
     7321 ccgcacagat cccggcgtgc gcgatttacg caccgggctc ctgcctcggg tgtctggcgg
     7381 tgaaccgctc cacaggccat ggatgaagaa cccgaaccct tggtagccat gcggctgcca
     7441 gtttgtttgc tttcgtccag gtcgtatcat ccttctggct cctgcgcctg agcgcccggc
     7501 gccagaggtt tgttacgtgt gtcctgaact tctgcatggt ggggaagttg cccggtaccg
     7561 agtgatagtt caggtatccc tgaaccactc tcctgagcca ttttccctgt tcggggattg
     7621 agtaatgcca gcgccttcgc agaccgtctt tgatggcttt cagagttgcc gtcatccgat
     7681 cccggcgggt ctttcgtatc agcatgaacc tgccgttgcg atctttcccg ctgatgtgcg
     7741 tgaacccgag gaagttgaac gtttctggtt tgccttttcc cctgatggca cggttttcgg
     7801 cagcgaagcg gccgaactcc atcagacggg ttttctccgg gtgaaccgtg agtccgaact
     7861 ccctcagtct gcgctgcatg gctatacgga agcgccgggc atcgtatcgt ttgtcgaacc
     7921 cgatgacgat gtcatcggcg tatctgacca ttaccacatt gcctgtggca tagcgacgtc
     7981 gccactgatg cgcccacaga tcgaagacgt agtggaggta tatgtttgcc agcagcggtg
     8041 agatgaccgc accctgtggg gtgccttcct ccgttgctcg ccattgaccc tcctccgacg
     8101 tcccggctgt gagccactta cgtatgagcc tgattaccct ccggtcgccg atccgatgct
     8161 ctgtgaacct gatcagccat tcgtggctca ccctgtcgaa gaactgactg atgtcggcat
     8221 ccagtaccca gtttacgtta gtgcgtacca gccctgtggc cagtgcgtcc agtgcatcgt
     8281 gctggcttcg cccgggtctg aacccgtatg agaaccccat aaagtcgttt tcatagactg
     8341 cgttcaggat tttcaccagc gcatactgga cgatcttgtc ctccagcgag gcgatgccga
     8401 gcgggcgttg ttttccatcc gcttttggga tgtagtgacg cctgccgggc tgcgccctgt
     8461 agctgccctg atgtagcctc cggtgcagat ctgttatgtt gttcttcatg tttccggcgt
     8521 agtccatcca cctgatgcca tccactccgg cggccgcttt cctgctcagg gagaggaatg
     8581 cggcttccag tgcttcgact gtcagcaggt ggaacaatgc tgtaaaccgt tctttcttcc
     8641 gctgcttcgc agcttcccgc acgcgtgaca gcctctgtga catgctttcc cggctctgtg
     8701 tccggcgcat gtgtggctgt tccgcgttcc ccttggcccc gctccttcgc tccactgact
     8761 ccgctccttt cgggttgttc gcctgcttcg ccgctactat gagcgagtcc gacttctcct
     8821 ctccgtacat caccggctat gactcctcgt cttcccggtg cgggccatct ccgacactgg
     8881 cagatggtca gaggggagat ctcccggttc ccgcgtagag atcgtattga catgccaggg
     8941 tctcagaccc cgccgggtcc atgtggcact cgcagtatcg caccctatga tgttgccttc
     9001 cgttaacagt acaacgtcgg cacccggtaa tttaatatac atttcgtggc tcaatggctg
     9061 gcctgtcaac acccctgtca acgcttcgcc ccatacctcg cggtatgcaa cgcatgactc
     9121 ggggaccttg tggattgctg gtccttcaat ggtcggggac tttcacccct tgatctctaa
     9181 ccggtctccc ggcgcacact gtacatatac acagtataat tattacctta ctcctcccgt
     9241 tttcaggtgt caatatatga gattagttct catcttcagg cacaaaaaag cgcccggagc
     9301 cacctgtaag cagatcgcgt cttttcccga aaatttctca tggcaacaaa ttttattatt
     9361 taagacattc atcacactta catttcagat ggtatagaca catctgaaag ctaactggca
     9421 tgaggagagg acctgcggtt aaactgttcg ggaaacctga gacgcccgga ggccactatg
     9481 acgcacctga aactgactcc cgctgaagaa aagcgcctgg cggacgcctt tgtcgctcag
     9541 gcactggcca tgggcctgaa agggaacaaa acgctgccaa aggcggtaaa acaacaggtg
     9601 cgggaaaggg tgcttcgtgc cattcaggac gaacgtgaag ccaggcgggc gcaggaaatg
     9661 cgtcgggaga aatacgaggc tacggaaacc accttcacct ggcagccaca gcgccgccgg
     9721 taaggggtat ttaccataac gtcgcattgc ccgcaccgct cagacggtct gagggcgttt
     9781 ccggcggcta cgggataaaa caggcaacaa accacccgga aaaccgccgg aaacgcgccc
     9841 ggtaaattgt aacccgctgc atgcctatgc acgcaactgc atggcgggcc gtttggtgta
     9901 aaaacgatac gataagccgt gtttatgtaa atgaacgtgg ccgtaaagag tgacaaatat
     9961 acatgtaaca ttttttactg acaggtcatc aacccgatgc gtttaatacc ctttatcctg
    10021 ctggcattgt gcagcccctt actcctgacg gcctgtaagc attccaccac ccagccgccg
    10081 aaaatttata ctctggataa gtcatctaaa atcagtagct acgctgcggc catcaagacc
    10141 gctgttgaaa tgcagatgtt tgaaccagag gcctatgcgg gaaaaatgtg caccgtgcgg
    10201 atatccatac aacgtgatgg cactctgaac agcgccattg ctgaaggagg agatccgaaa
    10261 ctctgtaaag cagcaatatc agccgttacc cacgccaaaa ttccgccggc accggatgaa
    10321 aatacctggc aaatattcaa aaacgcccct ctggatttca gaccatgaaa gcttttactc
    10381 tgacagtgct ggcggccatg ttcctcactc cggtacatgc gataccacta aaacccgtaa
    10441 ccacactgca gccaacctac ccggtacagg ccgcagcaaa acgcatcgaa ggtcaggtga
    10501 ccgccacgtt tgatattctg gacgatggac gagtggacaa cgtgtcgctt accggctgtc
    10561 accttttttt tcgggaaacg ctgacggcaa tgaggcactg gctatatccc gcagggcacc
    10621 cggcaatgcg acaggttata aattttgaat tcagtctgaa aaatgacggc ttacaggatc
    10681 cgcgaccagt caccacccaa aagctcaatg atgatggttg ccgccagtaa caggtccttt
    10741 aacccgcagg ctgcaatagt cctggatgat gagtgagcct gtagcagccc tgattagtgg
    10801 aagcgaagct gaaaaatggg cagcaaaagc cgtatttctg gcaaatagcg ccgccacgga
    10861 ttaaagtagt catgtgtaat acatatgact acaaaggggc ttttatgtca tcagcaacat
    10921 ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac gctggccgat gaccggcacc
    10981 gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata catcgaccgg gaagagaagc
    11041 gcagccagta cctgcgggac ggacaggccg catggcagca ctatcaggaa acgggtctgc
    11101 accttactgc ggaagaagcg gaagcctgga taagtacctg gggaactgag aacgagcagg
    11161 atgctccgcc atgccacagg taacgatttc cgcgctggca cagcgcgatt tacaacgcct
    11221 tcaggacttt ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg aggtgatcgt
    11281 ccgggctatc cagcaactga aaacattgcc agacattggc cgcccggtgc cgtttctgcc
    11341 gctggaatat aaggaactgg tgatcgggtt tggcgacagt ggctatgtga tgctctaccg
    11401 ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc aggcatcaga aagaatccgg
    11461 gtatccgggg gcagacagcc tctaataacc gatgacgggg gcgcaattca ttgcgtggcc
    11521 gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga aaaacgggta
    11581 aaattcagcg atacatttac cgtgagaaat tacacacctt tcacacccga taaaatcagg
    11641 ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc gcgggcatta
    11701 ccagaagaac gctatacaat catgtaaaac agggaaaggt taccgtgtca cgtgacggga
    11761 aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat gtgaatatcc
    11821 ctgagaaaca aattcccggc atttcacacc gtgaaaatac acaaaaaaat ttcccacagg
    11881 aacagcttct ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc accctgatgc
    11941 tggaagataa aacatcccgg gaagaagaac gccgccagca cgatgatgag cgccggaaac
    12001 tccaggccga agtggacaga ttgacaacag aactgacaca gaagaaaaaa cgcttctggt
    12061 ccggctggtt tagctgagaa cttaccgaag ggaatggaga acatgacgaa gcaggaaaaa
    12121 actgccctca atatggctaa attcattaag gatcagtcgc tgctgttgct ggaaaagctg
    12181 aacgagctgg acatggatac cccggccgac atgtgcgaac ggcttcacga ggatgcggaa
    12241 acactctggc aggtgatgcg ggataagctc ggggaagaat aacagcaccg gccatgccgg
    12301 tgctggcgtt attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt
    12361 tcaggcaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc
    12421 ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgttaatc
    12481 tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc
    12541 tccacctttc gatagaggta atcacacagc ttcattcctg tgggtttgtc ccgttcccat
    12601 tgcggatttt caccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca
    12661 tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca
    12721 tacgccccgg tctgccaggc gagcacc
//
