LOCUS       NZ_CP144141            10060 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Klebsiella pneumoniae strain KP8022 plasmid pKP8022-3, complete
            sequence.
ACCESSION   NZ_CP144141
VERSION     NZ_CP144141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39683583
            Assembly: GCF_036429575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Sequence characteristics of the clinical Klebsiella pnenmoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2024) Faculty of Graduate Studies, Hangzhou
            Medical University, Xi hu district, Hangzhou 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/07/2024 23:12:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,283
            CDSs (with protein)               :: 5,283
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 45 of 132
            Pseudo Genes (incomplete)         :: 85 of 132
            Pseudo Genes (internal stop)      :: 16 of 132
            Pseudo Genes (multiple problems)  :: 14 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP144141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP8022"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP8022-3"
                     /geo_loc_name="China"
                     /lat_lon="29.86 N 121.56 E"
                     /collection_date="2023-06-18"
                     /collected_by="zhangxiaofan"
     gene            complement(join(10036..10060,1..155))
                     /locus_tag="V2W15_RS27630"
                     /old_locus_tag="V2W15_27630"
     CDS             complement(join(10036..10060,1..155))
                     /locus_tag="V2W15_RS27630"
                     /old_locus_tag="V2W15_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(181..609)
                     /locus_tag="V2W15_RS27635"
                     /old_locus_tag="V2W15_27635"
     CDS             complement(181..609)
                     /locus_tag="V2W15_RS27635"
                     /old_locus_tag="V2W15_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(773..1087)
                     /locus_tag="V2W15_RS27640"
                     /old_locus_tag="V2W15_27640"
     CDS             complement(773..1087)
                     /locus_tag="V2W15_RS27640"
                     /old_locus_tag="V2W15_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1075..1353)
                     /locus_tag="V2W15_RS27645"
                     /old_locus_tag="V2W15_27645"
     CDS             complement(1075..1353)
                     /locus_tag="V2W15_RS27645"
                     /old_locus_tag="V2W15_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1528..1893)
                     /locus_tag="V2W15_RS27650"
                     /old_locus_tag="V2W15_27650"
     CDS             complement(1528..1893)
                     /locus_tag="V2W15_RS27650"
                     /old_locus_tag="V2W15_27650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(1890..2261)
                     /gene="tolA"
                     /locus_tag="V2W15_RS27655"
                     /old_locus_tag="V2W15_27655"
     CDS             complement(1890..2261)
                     /gene="tolA"
                     /locus_tag="V2W15_RS27655"
                     /old_locus_tag="V2W15_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2535..2780)
                     /locus_tag="V2W15_RS27660"
                     /old_locus_tag="V2W15_27660"
     CDS             complement(2535..2780)
                     /locus_tag="V2W15_RS27660"
                     /old_locus_tag="V2W15_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3107..4792
                     /locus_tag="V2W15_RS27665"
                     /old_locus_tag="V2W15_27665"
     CDS             3107..4792
                     /locus_tag="V2W15_RS27665"
                     /old_locus_tag="V2W15_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4802..5059
                     /locus_tag="V2W15_RS27670"
                     /old_locus_tag="V2W15_27670"
     CDS             4802..5059
                     /locus_tag="V2W15_RS27670"
                     /old_locus_tag="V2W15_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5144..5293
                     /locus_tag="V2W15_RS27675"
                     /old_locus_tag="V2W15_27675"
     CDS             5144..5293
                     /locus_tag="V2W15_RS27675"
                     /old_locus_tag="V2W15_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6777..8741
                     /locus_tag="V2W15_RS27680"
                     /old_locus_tag="V2W15_27680"
     CDS             6777..8741
                     /locus_tag="V2W15_RS27680"
                     /old_locus_tag="V2W15_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8741..9472
                     /gene="mobC"
                     /locus_tag="V2W15_RS27685"
                     /old_locus_tag="V2W15_27685"
     CDS             8741..9472
                     /gene="mobC"
                     /locus_tag="V2W15_RS27685"
                     /old_locus_tag="V2W15_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9479..10009
                     /locus_tag="V2W15_RS27690"
                     /old_locus_tag="V2W15_27690"
     CDS             9479..10009
                     /locus_tag="V2W15_RS27690"
                     /old_locus_tag="V2W15_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9792
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5397..5975)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8742..9469
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atcacctgcc agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta
       61 tccatgtcca gctcgttcag cttttccagc aacagcagcg actgatcctt aatgaattta
      121 gccatattga gggcagtttt ttcctgcttc gtcatgttct ccattccctt cggtaagttc
      181 tcagctaaac cagccggacc agaagcgttt tttcttctgt gtcagttctg ttgtcaatct
      241 gtccacttcg gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg
      301 ggatgtttta tcttccagca tcagggtgac agcctgccgg agatcggcca gttccttttg
      361 cattgccaga agctgttcct gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc
      421 gggaatttgt ttctcaggga tattcacatt tccgtataca cggatcagtt ctgacacatc
      481 cacgacaggg ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt ttacatgatt
      541 gtatagcgtt cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt
      601 tttcgccact gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta
      661 aatgtatcgc tgaattttac ccgtttttcc gttcaggatg tggttttctg acgcgctgcg
      721 cttgtcaaac cggcgacggc cacgcaatga attgcgcccc cgtcatcggt tattagaggc
      781 tgtctgcccc cggatacccg gattctttct gatgcctgac ggtgacgatc acaatctggt
      841 ccatttcccg gtcgtggcgg tagagcatca catagccact gtcgccaaac ccgatcacca
      901 gttccttata ttccagcggc agaaacggca ccgggcggcc aatgtctggc aatgttttca
      961 gttgctggat agcccggacg atcacctcac ctccttttct ggctgccagc cgatttttgg
     1021 ttttgagaaa gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct
     1081 gtggcatggc ggagcatcct gctcgttctc agttccccag gtacttatcc aggcttccgc
     1141 ttcttccgca gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc
     1201 ccgcaggtac tggctgcgct tctcttcccg gtcgatgtat tcggtgattg cctccagcat
     1261 tagcgcgtgc gctgagcggt gccggtcatc ggccagcgtt ttcagccggt ccttaagttc
     1321 ttcatcgagc ctgatggatg ttgctgatga cataaaagcc cctttgtagt catatgtatt
     1381 acacatgact actttaatcc gtggcggcgc tatttgccag aaatacggct tttgctgccc
     1441 atttttcagc ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc atccaggact
     1501 attgcagcct gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat tgagcttttg
     1561 ggtggtgact ggtcgcggat cctgtaagcc gtcatttttc agactgaatt caaaatttat
     1621 aacctgtcgc attgccgggt gccctgcggg atatagccag tgcctcattg ccgtcagcgt
     1681 ttcccgaaaa aaaaggtgac agccggtaag cgacacgttg tccactcgtc catcgtccag
     1741 aatatcaaac gtggcggtca cctgaccttc gatgcgtttt gctgcggcct gtaccgggta
     1801 ggttggctgc agtgtggtta cgggttttag tggtatcgca tgtaccggag tgaggaacat
     1861 ggccgccagc actgtcagag taaaagcttt catggtctga aatccagagg ggcgtttttg
     1921 aatatttgcc aggtattttc atccggtgcc ggcggaattt tggcgtgggt aacggctgat
     1981 attgctgctt tacagagttt cggatctcct ccttcagcaa tggcgctgtt cagagtgcca
     2041 tcacgttgta tggatatccg cacggtgcac atttttcccg cataggcctc tggttcaaac
     2101 atctgcattt caacagcggt cttgatggcc gcagcgtagc tactgatttt agatgactta
     2161 tccagagtat aaattttcgg cggctgggtg gtggaatgct tacaggccgt caggagtaag
     2221 gggctgcaca atgccagcag gataaagggt attaaacgca tcgggttgat gacctgtcag
     2281 taaaaaatgt tacatgtata tttgtcactc tttacggcca cgttcattta cataaacacg
     2341 gcttatcgta tcgtttttac accaaacggc ccgccatgca gttgcgtgca taggcatgca
     2401 gcgggttaca atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt
     2461 tatcccgtag ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt
     2521 atggtaaata ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc
     2581 tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca
     2641 cgaagcaccc tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc
     2701 aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga
     2761 gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac
     2821 cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa
     2881 gtgtgatgaa tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg
     2941 cgatctgctt acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca
     3001 tatattgaca cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt
     3061 atgttggctt taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga
     3121 cggtcgaggt ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt
     3181 gaacggtacg tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
     3241 gccgggctgg ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga
     3301 gtttggtaac ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg
     3361 tagctctggt ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc
     3421 tactccgggg gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt
     3481 tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat
     3541 ctacggtgtg ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat
     3601 gacgtcatta ccggccgatg cggtgacgga gactccggca agtactttac cactggacca
     3661 ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat
     3721 tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg
     3781 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa
     3841 aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg
     3901 tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
     3961 gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
     4021 acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga
     4081 ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat
     4141 tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc
     4201 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac
     4261 ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa
     4321 ggatattcag aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa
     4381 tgaatcctta tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa
     4441 ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa
     4501 ggggactaaa gactacggcc atgattatca tccagtacct aaaacagaag acataaaggg
     4561 acttggagag ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg
     4621 ggcccgatgg tatggcgata aaaaacgtaa aatttatgaa tgggattccc agcacggtga
     4681 gcttgaaggg tatcgtgcca gtgatggcga acacctaggc gcatttgatc ccaaaacggg
     4741 taagcaggtt aaagggccag atccaaaacg gaacattaaa aaatatcttt gagaggtaaa
     4801 tatggggctt aaattacgtt tgacatggtt tgataagaaa actgaagagt ttaaagggga
     4861 agagtattct aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt
     4921 aaaggataat attaataatg gtgagtttga tttgaagaaa gaatgggttc ctttactaca
     4981 gccatttttt aaaaatcaaa taaaacctga tgtggataat tgttatatag cttttgatta
     5041 tcgggacggt aactggtaac aaaaaattat caaggatgat ttgaaatact gacttccggt
     5101 gttcagggat gaatgccgga gtctttaaat acagggaaag tatatgaaaa aggtaaagac
     5161 tatttttctt tttatattga ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta
     5221 tatccgggat gttcagggcg gaacggtggc accatcgtcc tcctctgaac tgacggggat
     5281 cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaata
     5341 ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt
     5401 tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct
     5461 tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa
     5521 tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg tgctttcccg
     5581 ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggagtt
     5641 cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac cagtgtgtga
     5701 gctatgagaa agcgccacac ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg
     5761 gcagggtcgg aacaggagag cgcaagaggg agcgacctac cggaaacggt ggggatcttt
     5821 aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca
     5881 gatgcaccga aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa
     5941 aacgccaccg gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg
     6001 aatctgtggg taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt
     6061 agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg
     6121 cgctgatgtg ggatattgtc ccctgattaa gtctttctat tccctgacga tcaattatca
     6181 ggataggtga accatgtcgg tttttcgctg aacattttac gttaacctat ctctgttgct
     6241 tttttctcct tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga cttcagggga
     6301 aaagaccttg cttacgctgg ctggacgcca ccgacgcacg gtcttttccc ctctttctgg
     6361 ctctgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc
     6421 ctgaccaccc acaaaaatcc acggtgctgg acgcacctga cgaagcagca aagctgctcc
     6481 tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg
     6541 aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc
     6601 tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg
     6661 aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt
     6721 gcagcggggg ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt
     6781 ttaatacgga ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc
     6841 cgtgggtcag ctggctgtac cgggagtatt acagtctggc cggttttttt ctggttatcg
     6901 gcctgttttt cggtctggct gaactctggg ccggccgaat gcgttttgtg gggctgagcc
     6961 ataaggcgcg gcgggagtat gccgatcgct ataagcgcga taataaccgg actgtgccat
     7021 tttcggtgct ggcctcgctg gattcacccg ggcggatggt cagggcacgg cgacatgtct
     7081 ggaactacgc gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg
     7141 tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg
     7201 cagattatta cctgctgcag ccggctaacc tgttcgggat agcctggtgt gccggagggc
     7261 ttgtcgccgg cgtccttctg gcgcggctgc tggcgttcct gctgcttgat ggtcattttg
     7321 cggccgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc
     7381 ggaccggaga aatgacagac gtccgccacc tgcatttcgg ggagccggta ccggttaacg
     7441 cgctggccga tttttccacg gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg
     7501 atgagcaggg ccagccggtg ctggtccctc gtgatacctg gcgcaagacc aatattcaga
     7561 tcctcggcct gcccgggagc ggcaagagcg cgatggccac caacgcgctc attcgctgcg
     7621 tacgcgattt cggggacgct gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc
     7681 acgtcttccg ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg
     7741 cgcagctgaa tctgttccgc gatctcgacc agtacgccct gaagaatctg ctggttgccg
     7801 ggtttaacct gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag
     7861 cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg
     7921 cggcgtacgc gttgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg
     7981 agaacgtggc cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg gcggggataa
     8041 tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg
     8101 tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga
     8161 ggtggccgca cgcgagcatc atgctcgatg agattaaata tctgctgtcg aagtacgtgc
     8221 tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc
     8281 tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac
     8341 tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg
     8401 cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg
     8461 cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt
     8521 ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg
     8581 tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa
     8641 ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg
     8701 gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg agcagtacaa
     8761 cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga ccctgcgctg
     8821 gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc
     8881 ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc tggtccgggc
     8941 gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc
     9001 gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc cgtcaagggt
     9061 gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct ccatggaggg
     9121 ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc
     9181 ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc gcaccgtcaa
     9241 gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg
     9301 catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg tacagcgggc
     9361 gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga
     9421 gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat
     9481 ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat
     9541 gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt
     9601 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     9661 cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg
     9721 ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg
     9781 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     9841 taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct
     9901 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact
     9961 gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg
    10021 gcatggccgg tgctgttatt cttccccgag cttatcccgc
//
