LOCUS       NZ_CP143944             9119 bp    DNA     circular CON 08-FEB-2024
DEFINITION  Bacteroides thetaiotaomicron strain DFI.6.40 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP143944
VERSION     NZ_CP143944.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22167535
            Assembly: GCF_036419675.1
KEYWORDS    RefSeq.
SOURCE      Bacteroides thetaiotaomicron
  ORGANISM  Bacteroides thetaiotaomicron
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 9119)
  AUTHORS   Lin,H., Sundararajan,A. and Pamer,E.G.
  TITLE     17 beneficial commensal bacteria to re-establish healthy gut
            microbiome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9119)
  AUTHORS   Lin,H., Sundararajan,A. and Pamer,E.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2024) Duchossois Family Institute, University of
            Chicago, 900 E 57th St., Chicago, IL 60637, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-JUL-2023
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION + Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/07/2024 12:25:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,522
            CDSs (total)                      :: 5,425
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 43 of 71
            Pseudo Genes (internal stop)      :: 16 of 71
            Pseudo Genes (multiple problems)  :: 11 of 71
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143944.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9119
                     /organism="Bacteroides thetaiotaomicron"
                     /mol_type="genomic DNA"
                     /strain="DFI.6.40"
                     /isolation_source="fecal sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:818"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA:Chicago"
     gene            106..1131
                     /locus_tag="V3C11_RS27525"
                     /old_locus_tag="V3C11_27525"
     CDS             106..1131
                     /locus_tag="V3C11_RS27525"
                     /old_locus_tag="V3C11_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004295509.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1168..1398)
                     /locus_tag="V3C11_RS27530"
                     /old_locus_tag="V3C11_27530"
     CDS             complement(1168..1398)
                     /locus_tag="V3C11_RS27530"
                     /old_locus_tag="V3C11_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295510.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="hypothetical protein"
     gene            complement(1418..1870)
                     /gene="mbpC"
                     /locus_tag="V3C11_RS27535"
                     /old_locus_tag="V3C11_27535"
     CDS             complement(1418..1870)
                     /gene="mbpC"
                     /locus_tag="V3C11_RS27535"
                     /old_locus_tag="V3C11_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_004295511.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="mbpC"
     gene            complement(1827..2621)
                     /gene="mbpB"
                     /locus_tag="V3C11_RS27540"
                     /old_locus_tag="V3C11_27540"
     CDS             complement(1827..2621)
                     /gene="mbpB"
                     /locus_tag="V3C11_RS27540"
                     /old_locus_tag="V3C11_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_007560590.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="mbpB"
     gene            complement(2618..2917)
                     /gene="mbpA"
                     /locus_tag="V3C11_RS27545"
                     /old_locus_tag="V3C11_27545"
     CDS             complement(2618..2917)
                     /gene="mbpA"
                     /locus_tag="V3C11_RS27545"
                     /old_locus_tag="V3C11_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_004327437.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="mbpA"
     gene            complement(3251..3592)
                     /locus_tag="V3C11_RS27550"
                     /old_locus_tag="V3C11_27550"
     CDS             complement(3251..3592)
                     /locus_tag="V3C11_RS27550"
                     /old_locus_tag="V3C11_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin RelE"
                     /protein_id="WP_008782577.1"
                     /translation="MSFEVKTTPHFEREAKILAKRYKSFKADMKDFVESLEKNPMQGDE
                     LSPGIRKIRLAIVSKGKGKSGGARVITYTICASESEGRVYLVDVYDKSDFSTVSVSILK
                     KIISEQGII"
                     /name="toxin RelE"
     gene            complement(3582..3815)
                     /locus_tag="V3C11_RS27555"
                     /old_locus_tag="V3C11_27555"
     CDS             complement(3582..3815)
                     /locus_tag="V3C11_RS27555"
                     /old_locus_tag="V3C11_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005783253.1"
                     /translation="MQVIPLSKFRTNQTATLLRAIQGESVFLTSRIGDFKLVPVSVEEK
                     IATRIREGLNEVKRIEAGEQPAKSANAFLDEL"
                     /name="hypothetical protein"
     gene            4230..4628
                     /locus_tag="V3C11_RS27560"
                     /old_locus_tag="V3C11_27560"
     CDS             4230..4628
                     /locus_tag="V3C11_RS27560"
                     /old_locus_tag="V3C11_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005783251.1"
                     /translation="MKPTTYINWDGLKDIPFFYCDTKEDEENKDFDIYYQGRLVLHDYN
                     HCGHYLYTAAVLFSRIKNKTADWVNLRNLWILRDCVRENYNHGIGVDDIIFGENFDGEN
                     LDTLTPLTKKRFDYLCKRIKELDPYATI"
                     /name="hypothetical protein"
     gene            4643..5629
                     /locus_tag="V3C11_RS27565"
                     /old_locus_tag="V3C11_27565"
     CDS             4643..5629
                     /locus_tag="V3C11_RS27565"
                     /old_locus_tag="V3C11_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782578.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_227198504.1"
                     /translation="MARTIINNKQVFQSYVLTTAKYDFSPYEKRIMYRLIELAQKEIEG
                     IKLKDNLRKIAPTNFGREIIMPVSDILKDEQDKHYTIAKAAFRSLSEKHIEYEDDEVWS
                     YTPIITAPEIKKNSGSVKFYVFNNIWRCLLDFTKGFKKYELITAMKFKSAYAMRFYELM
                     SGQTKPLFVPLEGPDGLRERFYLQGKYEKVNDFRRKVIDVAKKELDESSPYSFVAKEEK
                     AGKKIIGWTFFPVFYENREDPALQEQARMAKVTARLQLENNVYDYLKFSFDFKSDEINK
                     NKKTLIEGQNRIPDFMGFLGELKKGARLAENPKGYVIGAIKRKLKGI"
                     /name="replication initiation protein"
     gene            5698..6351
                     /locus_tag="V3C11_RS27570"
                     /old_locus_tag="V3C11_27570"
     CDS             5698..6351
                     /locus_tag="V3C11_RS27570"
                     /old_locus_tag="V3C11_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149886708.1"
                     /translation="MKKFLLSLATAFSFVLFLGSCTNEEDINNNGYSDKEKAKIETLMN
                     LFDSYGWELDTTVSIEQRNKELLEMDYEKTKSFLEYMSNGIEFDNFEPTQQSEDNAPKA
                     LSNTRSTMTFPIYGSHSSAVASSQTTMILSYDGPKPSSVTIQSTSVSSNPATTWTPDEY
                     GSFNFSGNKCDNIKATGMIKYGSIYKHKYEMVGWCSKNSSGIVDDGKITGFHAI"
                     /name="hypothetical protein"
     gene            complement(6393..6842)
                     /gene="mbpC"
                     /locus_tag="V3C11_RS27575"
                     /old_locus_tag="V3C11_27575"
     CDS             complement(6393..6842)
                     /gene="mbpC"
                     /locus_tag="V3C11_RS27575"
                     /old_locus_tag="V3C11_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpC"
                     /protein_id="WP_005783248.1"
                     /translation="METSSNPKTEDYTYKQGQYSPRKRKDTTPKVNPQLAVEIVFQELK
                     RVEAYTKRIEDATAKKVEIDRGSLESAENRLKNVLVDFERQGNRMKNGSYVDKKVSMYS
                     ILCAFFSLLFACLMCYLWVDAAKDRDNYKRYYEYFQEMDKQKSGE"
                     /name="mbpC"
     gene            complement(6799..7593)
                     /gene="mbpB"
                     /locus_tag="V3C11_RS27580"
                     /old_locus_tag="V3C11_27580"
     CDS             complement(6799..7593)
                     /gene="mbpB"
                     /locus_tag="V3C11_RS27580"
                     /old_locus_tag="V3C11_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpB"
                     /protein_id="WP_005783247.1"
                     /translation="MIAKGKSISHGTAALEYDLAKEINGEAAATEIHRHELFGCTGEEM
                     VQEMKPYFVDFPNVKNNCLRFEVSPSVEESAGMTDADWAKLGNDFMQRMGLMNHQYVIV
                     KHSGTEKNSRQAHLHILANRVSLSGELYKDNWIGKRATEAANSIARERNLVQSKDIGKA
                     NREEIKQAMDSVLTRMQGFDLAGFSRELGKLGFKVREARASTGKLNGYYVEARSGTEYK
                     ASEIGKGYTLAHIEKTQKKLKYNSISRNYGNILKPKDGGLHL"
                     /name="mbpB"
     gene            complement(7590..7889)
                     /gene="mbpA"
                     /locus_tag="V3C11_RS27585"
                     /old_locus_tag="V3C11_27585"
     CDS             complement(7590..7889)
                     /gene="mbpA"
                     /locus_tag="V3C11_RS27585"
                     /old_locus_tag="V3C11_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbpA"
                     /protein_id="WP_005783246.1"
                     /translation="MEVNRTEKITFRCTALEKAALAEQAARCGISTSEYCRTLALGGRP
                     KERYTEEERELFREIARLKGTLQRLNNYFGGRQYREVFEENQALINELKKILSR"
                     /name="mbpA"
     gene            complement(8081..8278)
                     /locus_tag="V3C11_RS27590"
                     /old_locus_tag="V3C11_27590"
     CDS             complement(8081..8278)
                     /locus_tag="V3C11_RS27590"
                     /old_locus_tag="V3C11_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295503.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(8289..8564)
                     /locus_tag="V3C11_RS27595"
                     /old_locus_tag="V3C11_27595"
     CDS             complement(8289..8564)
                     /locus_tag="V3C11_RS27595"
                     /old_locus_tag="V3C11_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="WP_004327436.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(8561..8812)
                     /locus_tag="V3C11_RS27600"
                     /old_locus_tag="V3C11_27600"
     CDS             complement(8561..8812)
                     /locus_tag="V3C11_RS27600"
                     /old_locus_tag="V3C11_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004295507.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     Typing          complement(6803..7593)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.591"
                     /coverage="100.0"
                     /evalue="2.35e-171"
ORIGIN
        1 ctttgggagt ttaaaagtaa attagtcccc acactttggg agttttaccc ctcttttttt
       61 gttttaatgt ggggattgtg tatatttgca gcaaatcaat cgtttatgaa aaagaaacta
      121 cctattacca agaataaaga tgttgttgtt tcatgggtat atacatggtc aaaacagcaa
      181 gacatgtcca tacacgaaca aagaatagtt cttcgcatac tggaagcgtg ccaagctgaa
      241 ctaaagggag taaaactgaa agattatgca ggcacgaaac gcaagtttga gcatggactt
      301 tgggatgttg atgcacaaat gcatgtatct gacgttattt tttccggacg tgattacaat
      361 gaaatcatag ccgcactcga ttctttggca ggacgttttt ttacttacga agatgacgag
      421 gaatggtgga agtgcggatt tatatctaac ccaaaatata aaaaacgcac tggcattata
      481 acctttcgtg tgtctaacga cctttgggat gtgtttacca agttcgccaa ggggtacaga
      541 gaatttgagt taaacaaggc tcttgcgcta cctactgggt attcccttag gttttacatg
      601 ttaatgagtg ggcaggtgta ccctttggat atatcccttg aaaacttgaa agaccgtctt
      661 ggcatacctg cggacaaata caaggacaag aacggaaaag acagaataga tcattttgag
      721 gaaagagttt tgaaaccagc aaaggctgcg cttgatgaga gctgcccata cactttcaac
      781 tacgtgaaag tgcgtgaaaa cccaaacaac aaacgtagca aggtgaccgg gtttaggttc
      841 tacccggtct atcaaccaca gttcagagat gaagaactgg aagggaaaga attgcaagca
      901 aaggttacag cacgatatca gatagacagc catgtgtacg agtacctccg ttattcctgc
      961 ggcttcacat cggaagagat aaaccggaac aaagagacat tcataacggc acaagaaaag
     1021 ataaccgacc ttatcggaga actggctctc ctaaacggaa aatcacgaga aaagaacaat
     1081 ccgaaagggt ggattataaa cgcccttaaa gggaaaatca aggataagta acaagattac
     1141 ccggctgaaa cactccgggt aatcttgtca aatcagagga acgatttcca tttctacacc
     1201 aagttctttc atctgctgtt gtatttcctc gctgcttagt ttctctctcg ccagtgcaag
     1261 cccatgaaag gcgtcccagc ctgccggctt cgaaacgata ctaccgttta ggacggcatg
     1321 ggtgatttcg ccgccggact tcgcaccctt gttcagtatg aagaagaacc ggggttcttc
     1381 gtgttccgtt gttcccataa ttcgtaaagt tagatgttta tttattcccc ttttgttccc
     1441 ttgcctgctc ttggtagtat tcataatact gcttgtagtt gtcccggtct ttggctgcat
     1501 ccgtccaaag atagcacatg aaacaagcga acaataggga gataacagca caaaggattg
     1561 agtagaacga aattcgtttg tccacataac cgccgttttt cattctgtac ccctgccgtt
     1621 caaagtccgc caacacgttt tttaggcgat tttcggcact ttcaaggctt ttcccgtcta
     1681 tttgtacctt tcgggctgtt gcgtcctcta tgcgcttcgt atagacttca acacgcttta
     1741 gttcttgata caccagttct accgcaagct gcgggtttac ccttggcgtt gtatcttttc
     1801 ttttccgggg gctgtactgt ccctgcttaa cgttggaagc tgccgcttcc gggtgtgagt
     1861 ttgtttccat gagatatgtt cattgagttg cacttcagtt tgctttgtgt ccgctcgata
     1921 tgggcgagag tgtagccttt ccctatctcg cttgccttat actccgtacc gcttcgggct
     1981 gtgacatagt agccgttaag tttcccggtg cttgccctgg cttcccggac tttaaaacct
     2041 ctcttaccaa gttcttcctt gaatttggta aagtcaaagc cctgcatctt cttcaatacg
     2101 ccgtccatgg cttccttgat ttccgctttg ttgactttgc caatgtcctg cgactgtaca
     2161 aagttccgtt ccttggctat ggcattggcg gcttccgttg cctttttccc tatccagttg
     2221 tcccggtaca gttccccgga aagggaaacc cggtttgcca gtatgtgcag gtgggcttgc
     2281 tctttcttgc tctccgtacc gctgtgccgg atgatgatgt actggtggtt cgccaacccc
     2341 atgcgctgca tgaagtcgtt accaagttcc gcccagtccg catccgtaaa ggtggcgctt
     2401 tcctctatcg aggggctgac ctcgaaacgt aggcagttgt tttttacgtt cggaaaatca
     2461 atgtgatacg gtttcatttc ctgcaccatt tccgcaccag tacacccata aagttcatgc
     2521 cgggctatct cggtggctac tgcctgcccg tttatctctt tggcgaggtc gtactccaat
     2581 gccgccacac catgtgatat gcttttccct ttccctatca tcgtgagagt atttttttga
     2641 gctctgtaat tagtgcccgg ttctcctcga acacttcccg gtactggcga ccaccgaagt
     2701 agttgttcag ccgttggagc gttcctttca actgggctat gtcccggaga agctgccgtt
     2761 cctcctcggt gtacctctcc cttgggcgcc ccccaaggga caaactccgg cagtattccg
     2821 atacgcttag actgcaccgg gctgcctgct ccgcaagtgc gtccttttca agttcggtac
     2881 accggaatgt aatcttttcg ttccgtctta gttccatgcc acaaaggtaa tcttaaaatc
     2941 tttttgagca aagcgagaaa agcaagaatt gtccgacaag gacacttctt gctatatata
     3001 cataccgcat gggaatacac acagcactaa cacactgcat agccacacat tttttttctt
     3061 ttttccctgc cattcccaca acgaaacacg gagagcggtc aagggttcga gcggaaaaaa
     3121 ccgcacagcg aagctgttca ggattttttc agatccgaac ttgcgaagcc cttgacggct
     3181 tcggagtgtg gagttacctt tgcagactga aaagagaaaa aacactgttc gcttcactaa
     3241 cgctgactgg tcaaatgata ccctgttcag atatgatttt tttaagaata gatacactta
     3301 cggtagagaa gtctgactta tcatagacat ccacaagata aacacgacct tcactctccg
     3361 atgcacaaat cgtgtatgtg ataacacgtg ctcccccaga cttacccttc cccttagaaa
     3421 caatggcaag tcttatttta cggataccag ggctcagttc atccccctgc atggggttct
     3481 tttccaatga ctcgacaaaa tccttcatgt cagctttaaa agacttatac ctttttgcaa
     3541 ggattttcgc ttccctctca aaatgtgggg tggtctttac ttcaaagctc atcaagaaat
     3601 gcgtttgcgc tcttggctgg ctgttcacca gcttcgattc gtttcacttc attcaagccc
     3661 tctcttattc gagtggctat tttttcttct acggacactg gcaccagttt aaaatcaccg
     3721 atacgtgaag tcagaaagac ggattctccc tgaatagcac gaagaagggt cgctgtctga
     3781 ttcgtccgaa atttgctcaa tggaattact tgcatatatc ttaaactttt ttgattctga
     3841 tacaaatgta accattgtta ccgatattgt aacaaaatta acatacttta acacgcagga
     3901 aaaagcttca ataaccgcac cccccttacg agggggcttt gtctttgttt tccctattgt
     3961 ttttcgtccg ttttttccga aagttgggaa gcaatctcaa ccttatgagc ttgtttctat
     4021 cttaacatcg actatctatg tcctatcctc gcgcgcgcgc attatgcgag aaaaacaccc
     4081 tctaaatacg acatttttga tactttaata cgacattttt gatacttttt tgaaggggta
     4141 aaaacgacat ttttgatact ttatgcgtct atccattgta ttagtcgttt ttaataatta
     4201 cctttgctta aacttttaaa tcagcactta tgaaaccaac tacttacatt aattgggacg
     4261 gtttgaaaga tattccgttc ttctactgcg atacaaagga agatgaagaa aacaaagact
     4321 tcgatatata ctatcaaggg aggctggtgc tgcatgacta caaccattgc gggcactacc
     4381 tctacacagc tgctgtgctg ttcagcagaa tcaagaacaa aacggcagac tgggtgaacc
     4441 tgcgaaacct ttggattctg cgtgattgtg tgcgtgagaa ctacaatcac ggcatagggg
     4501 tggacgacat tatctttgga gaaaattttg atggcgagaa cctcgataca ctcacaccac
     4561 ttacaaagaa acgatttgat tatttgtgta agcggattaa ggaacttgac ccatatgcaa
     4621 caatctaaaa aaggcagcat ctatggcaag aactataata aacaataagc aagtctttca
     4681 atcttatgtt ctaactactg ctaaatatga tttttcgcca tatgaaaagc gcataatgta
     4741 tagactaatt gagcttgctc aaaaagaaat tgaagggata aaattaaaag ataacctccg
     4801 aaaaattgct ccaacaaatt tcggcagaga aataatcatg cctgtatcgg acatactgaa
     4861 agacgaacaa gacaagcact acactattgc caaagctgca ttcagaagcc tatctgaaaa
     4921 gcatatagag tacgaagatg atgaagtatg gtcatacact cctatcataa ctgcacctga
     4981 aataaagaag aatagtggct ctgtaaagtt ctatgttttc aataatattt ggaggtgctt
     5041 gcttgatttt accaaaggat ttaagaagta tgaacttatt accgccatga aatttaaaag
     5101 tgcttatgct atgcggttct atgaactaat gtctggacaa acaaagccct tatttgtgcc
     5161 attagaagga cctgatgggc ttcgtgaacg attctatttg caaggaaagt atgaaaaagt
     5221 aaacgacttt cgcagaaagg ttatcgatgt agccaaaaaa gaactcgacg aatcttctcc
     5281 ttattcgttc gtagctaagg aggaaaaagc tggaaaaaaa attattggct ggactttctt
     5341 ccctgtattt tatgaaaaca gagaagaccc tgcgctgcag gaacaagcgc gcatggctaa
     5401 agttacagca cggctccagc tggaaaacaa cgtatatgac taccttaaat tctcctttga
     5461 cttcaagtct gatgaaatca acaaaaacaa gaaaaccctt atcgagggac aaaaccgtat
     5521 tcccgatttt atggggttct tgggagaatt gaagaaaggc gcacgactag cagaaaaccc
     5581 caaaggatat gttatcggcg ctattaagag aaaactaaaa ggaatctaac agaaagagga
     5641 caacataaca aataatttct taaatttgcc atatctaaac tttagatgta tactaatatg
     5701 aaaaagtttt tattatcatt agctactgct ttttcttttg tgttattctt agggtcatgt
     5761 accaatgaag aggacataaa caacaacggt tattccgata aagaaaaagc taaaatcgaa
     5821 acattgatga atttgttcga ttcttatggt tgggaacttg acacgactgt tagtatagaa
     5881 cagcgtaaca aagaactttt agaaatggat tacgaaaaga ctaaaagttt cttggagtac
     5941 atgagtaatg gtattgagtt tgataatttt gaaccaactc aacaaagtga agacaatgca
     6001 ccaaaagcct taagcaacac aagatctact atgacctttc cgatatatgg tagtcatagt
     6061 agtgctgtcg cttcatcgca aaccactatg attttatctt atgacggtcc aaaaccttcc
     6121 agtgtgacga tacaaagtac atcagtatct tcaaaccctg caacaacttg gactcctgat
     6181 gaatacggtt cttttaactt ttcaggtaac aaatgtgata atataaaagc cacaggaatg
     6241 attaaatatg gcagtatcta taaacacaaa tacgaaatgg tagggtggtg ttcaaaaaat
     6301 agttcaggta ttgttgatga cggaaaaata actggttttc atgcaatata gccaaatgat
     6361 aaaatatatt ataaacaaaa aagcggagaa tactactctc cgcttttttg tttatccatt
     6421 tcttgaaaat actcataata cctcttgtaa ttgtccctgt ccttggctgc atccacccac
     6481 agataacaca tcagacaggc aaacaatagg gagaaaaaag cacaaaggat tgagtacatt
     6541 gaaactttct tgtccacata gctgccgttt ttcattctgt ttccctgccg ttcaaagtcc
     6601 accaatacgt ttttcaggcg attttcggca ctttcaaggc ttcccctatc tatttccacc
     6661 ttcttggctg ttgcgtcctc tatgcgcttt gtataggctt ccacgcgctt caattcttga
     6721 aacacaattt ctaccgcaag ctgcgggttt actttggggg tggtgtcctt tctctttcgg
     6781 gggctgtact gtccctgctt ataggtgtag tcctccgtct ttgggtttga ggatgtttcc
     6841 ataattccta ctgattgagt tatacttcaa ctttttctgt gtcttctcga tgtgggcaag
     6901 cgtatagcct ttccctatct cgcttgcctt gtattccgtt ccgctccggg cttccacata
     6961 gtagccgtta agtttgccgg tactcgcacg ggcttccctc accttgaaac ccagttttcc
     7021 gagttcacgg ctgaatccgg caaggtcaaa cccctgcatc cttgtaagga cactgtccat
     7081 agcctgtttt atctcttccc tgttggcttt gcctatgtcc tttgactgta ccaaattccg
     7141 ttctctggct atgctgttgg cggcttctgt ggctctcttc cctatccaat tgtctttgta
     7201 gagttccccc gataacgaca cacggtttgc cagtatatgc aggtgggctt gtctgctgtt
     7261 cttctccgtt ccgctgtgct tcacgatgac gtactggtga ttcatcaagc ccatgcgctg
     7321 catgaagtcg ttgccgagct tcgcccagtc cgcatcggtc atgcctgcac tttcttccac
     7381 tgaggggcta acctcaaacc gaaggcagtt attctttacg ttcggaaaat caacaaagta
     7441 aggtttcatc tcctgcacca tctcttcgcc tgtgcagccg aaaagctcat ggcggtgtat
     7501 ctcggtggct gctgcctccc cgtttatctc tttggcaagg tcgtactcca gtgctgccgt
     7561 gccatgagat atgctctttc cttttgctat catcgtgaaa gtatcttttt aagttcattg
     7621 attagtgcct ggttctcctc gaacacttcc ctgtactggc gcccaccgaa gtagttgttc
     7681 agacgctgga gtgtcccttt cagtcgggct atctcccgaa acagttccct ttcttcttcc
     7741 gtgtacctct ctttgggtcg tccgcccagt gcgagcgtac ggcagtattc cgatgtgctg
     7801 ataccgcatc gggctgcctg ttccgcaagt gccgcctttt caagtgccgt gcagcggaat
     7861 gttatcttct ccgttcgatt tacttccatg tcgcaaaggt aatcttaaaa tccttttgag
     7921 caaagcgaga aaggcaagaa tgtccgacaa ggacacttct tgctatatat acacatgccg
     7981 catgggaata catccacgcc aaccgcccag caaaaagccc cggcgtgtta gaaaatcccc
     8041 cacagcctaa caaattctac cccatttcta cccaagggga ttacacgccg cagaagtcat
     8101 ccgggaacat gtcttccagt tcgtccaaat caatggtttc ttcttcggac acctccaccc
     8161 attcaatgtt ttctttccct cttatcgcac atgagtacat ctcggcggcg gcaagagaag
     8221 agaacacctc gtgcataggc tcgccgttct ctaaccagta aacacaatat tcgaccataa
     8281 cagttttatc atttatcgtt gtaatgcccc tctacctcca agatgtagac ctctaccact
     8341 tcctcgtaaa tgtcatagat tatccggtca tgggcggtaa tgtgtcgaga ccatgtaata
     8401 tccccaccgc cccttaacgc ttcgggatgc ccaagcccag tcttagggtg ctctaacaat
     8461 tctttgtaga ttttcttata cttcttgaac aaattggggt ttgatttctt ccatttggca
     8521 atcaccttat ccaccccgtc ggaaacccta attgtgtaca tcatagacta tcgaaataag
     8581 cgtcaatatc ctcactgctt ttgagggtaa cacactttcc ggatttgatt tcttcccttg
     8641 ccttgtcaat ccttgcttgc agctccgggg ttatcatcaa atcttcacga ccaactttta
     8701 ccaaagcgta aatctcgttt ttcctgcgaa ttagtacctg ctcgccattg tcagctttgg
     8761 tgaaagacgc tgcgaggttg ttacggtatt ctcttactga taatgcttcc atatctgttt
     8821 gcttttaaat tcagcacaaa gatagctata tttcaataaa atacaaacat tttgtacaca
     8881 aacgtgtaca cgccataaaa acccgtttcc aatcctaccg cccgttggtt ggttttgctt
     8941 tgctcttttt ccctatcgtt tttctttttc cgacagtaaa tcaaccactt agctctataa
     9001 attccccttg tctgttataa gcatcgtctg taatatgctc tatcacttct tcgcaaacgc
     9061 ccataaataa agggattaga acatgtgttt ccccacactt tgggagttta gtccccaca
//
