LOCUS       NZ_CP143924             4225 bp    DNA     circular CON 07-FEB-2024
DEFINITION  Klebsiella pneumoniae strain Kp2-1 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP143924
VERSION     NZ_CP143924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39665667
            Assembly: GCF_036417615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Hu,G.
  TITLE     Genome of Multidrug-resistant and ESBLs Klebsiella pneumoniae
            strains from Pharmaceutical Wastewater Treatment System in
            Guilin,China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Hu,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2024) Key Laboratory on Biosafety, Nanjing
            Institute of Environmental Sciences, Ministry of Ecology and
            Environment, Xuanwu, Nanjing, Jiangsu 210042, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-DEC-2023
            Assembly Method                   :: ABySS v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/06/2024 23:18:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,934
            CDSs (total)                      :: 5,810
            Genes (coding)                    :: 5,661
            CDSs (with protein)               :: 5,661
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 57 of 149
            Pseudo Genes (incomplete)         :: 92 of 149
            Pseudo Genes (internal stop)      :: 27 of 149
            Pseudo Genes (multiple problems)  :: 22 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143924.1.
            Bacteria and source DNA available from wastewater.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp2-1"
                     /isolation_source="wastewater"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Guilin"
                     /lat_lon="25.240638 N 110.342261 E"
                     /collection_date="2023-03-07"
                     /collected_by="Hu Gang"
     gene            join(2983..4225,1..8)
                     /locus_tag="V2161_RS29650"
                     /old_locus_tag="V2161_29650"
     CDS             join(2983..4225,1..8)
                     /locus_tag="V2161_RS29650"
                     /old_locus_tag="V2161_29650"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            1..810
                     /locus_tag="V2161_RS29655"
                     /old_locus_tag="V2161_29655"
     CDS             1..810
                     /locus_tag="V2161_RS29655"
                     /old_locus_tag="V2161_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(1030..1161)
                     /locus_tag="V2161_RS29660"
                     /old_locus_tag="V2161_29660"
     CDS             complement(1030..1161)
                     /locus_tag="V2161_RS29660"
                     /old_locus_tag="V2161_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            1575..1766
                     /locus_tag="V2161_RS29665"
                     /old_locus_tag="V2161_29665"
     CDS             1575..1766
                     /locus_tag="V2161_RS29665"
                     /old_locus_tag="V2161_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032446876.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNEFDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1766..2167
                     /locus_tag="V2161_RS29670"
                     /old_locus_tag="V2161_29670"
     CDS             1766..2167
                     /locus_tag="V2161_RS29670"
                     /old_locus_tag="V2161_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032446877.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLSSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1053..1453)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.812"
                     /coverage="98.0"
                     /evalue="5.92e-171"
ORIGIN
        1 gtgagtaagg ataaaaaaga aaggtccaaa aatagatacc atcagttggt agaaaagata
       61 tttttcgata aatatgttga tggtgactta gaggttttgt ttgaaaggtt agatataaac
      121 agtgctgcaa aagatttagg tatagaactc ccatctaatg ttggtgatgt tatatattca
      181 atcagattta gaacgcctat gccagaaagt attctgttga ctcagccaga aggtaaggag
      241 tggattattg agctggctgg taggtcaaag tatcgattta gattagtaac tattagtagg
      301 attttgccaa atcctaatat ggtggcaata aaaattccag acaatacccc ggagataatt
      361 acaggttatt ctttagacga cgagcaggca ttgctggcga aggtgagata taaccggcta
      421 atagatatat ttctaggtat aaccacatat tctttacaga atcatttaag aactacggtg
      481 aagggaattg gtcaaataga gatcgatgaa ctttatgtag gtatagataa atacggttgc
      541 cattatataa taccagttca agcaaaaggt gggacagatc aaatatcaat ggtgcaaaca
      601 tcgcaagata cagcgtggtg tgaacaaaaa tacccaaatg ttagatgtag agctatttcc
      661 gctcagtttg tttcgagcga tttgattgca ctgtttgagc taaagataga cgaaggacaa
      721 gtgaaattga ttgaggaaag gcattacaaa ctggttccgg taaaagagtt agatagctct
      781 gacatctcaa actaccgcat gtctgtataa tttctgagag gatcttcttg agatcctttc
      841 gttctagccg taatctccta agccgtaaac aaaaaaacca ccgctaccaa cggtggttcg
      901 tttgccggaa tttagagcta ccaactcttt cttccgaagg taactggctt tcacggagcg
      961 cagataccaa aactgtcctt tcagtgtagc cgtagtcagg ccaccacttc aagaactctg
     1021 tagataactc tactccgctc cgtgaatccg attaccagtg gctgctgcca gtggcgcttt
     1081 tgcatgtctt ttcgggttgg actcaagacg atagttaccg gacaaggcgc agcagtcgga
     1141 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     1201 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     1261 aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttata
     1321 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat tccgtgatgc ttgtcagggg
     1381 ggcggagcct atgaaaaaac ggccgctttg cggcctttcc ttcttcccca tttgaccgct
     1441 cggtcttctc gagtttttat acgcatgctc ctgccgctcg ccgcagccga acgaccggag
     1501 cgtagcgact gagtgagcga ggaagcggaa aagagactgg ttttacactg agcactgacg
     1561 ctctgaggcc atttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc
     1621 agaatcagtc tctgctcctg ctggagaagc ttaacgagtt tgatctcgat gtcgaggccg
     1681 acctttgtga gaaactccac gatgatgcgg agcatctttt ccgtacactg tcctcgagac
     1741 tggatgcatt acaggatggt aactgatggc cagagtgtct attagtgagg ctgcccgact
     1801 gacgggtaag agtagaacaa cactgcacag gttaattaaa gcaggtgacc tgtccacctg
     1861 tagcggtgag cgcaatgcaa aaatggtaga tacttcagag cttttacgtg tctttggtcc
     1921 ctttgagcaa ccgaagggtg aacaccattc tggacaggtc tctggacagc gtgatacagc
     1981 ctctgctgac cagagtgaac aggtgattcg tcatctgaaa caggagattg aacacctgaa
     2041 caccttactt tcgtccaagg attcgcacat tgacagcctg aaacaggcca tgctccttct
     2101 tgaaaatcac caggcaaaat taactgaatc cccggcgcca tggtggaaat tctggaaaaa
     2161 atcctgatcc tgccatgctt gagttttcct ctccggccat cccggggagt tcctgaacct
     2221 tcccaaaaat cgccagatct ctgaacgctc ctcacgacta aaaataccca cacactacgc
     2281 aaaattaaaa ttttgcagat ttgtatttaa tatcagtggt ttactttcct gaaatttcgt
     2341 tttttaaatt ttatttacat agatccgact ctatgacgac tcgtcataga gtccccggcg
     2401 agtcgtcata gagtcggtaa cgactcagga ctataaaaac aatgagttaa taacgacccg
     2461 actctagaca cgactccccg tttggttgtt ttttagactc ggcgactctg ggtacgactc
     2521 tagtgaagag tcgccgggga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat
     2581 tgccggccgt cgacgaacac gattcctgaa cgtgctgaaa accggttttc cggctgcgcc
     2641 ggcaacgctt cacggccacg caataaattg cgtccccgtt caccgttttc ggatgggaag
     2701 aacgccggaa acaggactta ccatggctga atggcgtgaa ttttcgcgct acgcacttgc
     2761 gtgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg
     2821 gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tgaggccgtt ttagcggtgc
     2881 gggcaagaga tgttatgttc aatcgaaatc agaaaaacga tcggtaaagc gatcgttttt
     2941 ctgatagcat catgttgatt ttatataacg tgggtgtaaa gaatgactag accgattgct
     3001 gttgatttat ttgctggagc gggtgggctg agtctcggtt tcgagcaagc tggctttgat
     3061 ataatggcat cagttgaaat agatccaata cattgctctg ctcacaaata taactttcct
     3121 atgtgcaaaa cgatatgtag aagtgttgtt gatgttaccg gagaatacgt tagggaagtt
     3181 tcaggtattg gtgagaaaga gattgatgta gtgatcggag gcgctccctg ccaaggcttt
     3241 tcattaatag gaaaacgcgc gcttgatgat gatagaaaca aactggtcta tcactatgta
     3301 cggctggttc ttgagctaaa gcctaaatac tttgtttttg aaaatgtaaa agggttgacg
     3361 gttggtaagc ataagcagtt tttagtagag ataatggatg catttaaaga tggcgggtac
     3421 gacgttgtaa gcgattataa agtgctaaac gcagctgatt atggagttcc tcaagatcgc
     3481 cgtcgtcttt tcttaatggg gggaaggaaa ggaattgctc tgccatcata ccctgagcca
     3541 ttatccatta gaacaacggt tggggacgct attggggata ttcctgatgc ggaaaaatat
     3601 cctgagctat ggggtagaga ttgggtttat gctaaatttg gtgagcaatc actatactca
     3661 tcctatttgc atggcgtagg aaaagatcct agtgatttta gctacccaag gatttttaat
     3721 aaagacaaat taacgtgttc tttactgaca gatcactctg acatttctag agggcggttt
     3781 gttgaaactt tgcctgacac ggtagaacca atcagcaggt ttaaaaagct ctcgatgaat
     3841 gggctatgta atactttaag ggcaggaaca gcaagtgaca gaggtgcttt tacatctcct
     3901 cgtcctatac atccgatcta tcctagggtc atcacggtca gagaggcagc aagactgcat
     3961 tcatatcctg attggttcag atttcatgtg actaagtggc atggatttag acaggtcggt
     4021 aatagtgttc ctcctatgct agctagaagt gttggcttgc agattatgaa ggttcttggt
     4081 gttacgccaa aaaaaccaac gaagaaaatc aaactcggtg atgaaagttt ggttgctatt
     4141 tctatgtccg atgctgcatc tatgtttggt gtgtcatcaa atgtaattgc taagagaata
     4201 aaaaatagta acaaggaggt tgttt
//
