LOCUS       NZ_CP143914             4225 bp    DNA     circular CON 07-FEB-2024
DEFINITION  Klebsiella pneumoniae strain Kp3-8 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP143914
VERSION     NZ_CP143914.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39664788
            Assembly: GCF_036417415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Hu,G.
  TITLE     Genome of Multidrug-resistant and ESBLs Klebsiella pneumoniae
            strains from Pharmaceutical Wastewater Treatment System in
            Guilin,China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Hu,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2024) Key Laboratory on Biosafety, Nanjing
            Institute of Environmental Sciences, Ministry of Ecology and
            Environment, Xuanwu, Nanjing, Jiangsu 210042, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-DEC-2023
            Assembly Method                   :: ABySS v. 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/06/2024 23:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,854
            CDSs (total)                      :: 5,730
            Genes (coding)                    :: 5,593
            CDSs (with protein)               :: 5,593
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 55 of 137
            Pseudo Genes (incomplete)         :: 81 of 137
            Pseudo Genes (internal stop)      :: 27 of 137
            Pseudo Genes (multiple problems)  :: 21 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143914.1.
            Bacteria and source DNA available from wastewater.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp3-8"
                     /isolation_source="wastewater"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Guilin"
                     /lat_lon="25.240638 N 110.342261 E"
                     /collection_date="2023-03-07"
                     /collected_by="Hu Gang"
     gene            271..402
                     /locus_tag="V2L73_RS29250"
                     /old_locus_tag="V2L73_29250"
     CDS             271..402
                     /locus_tag="V2L73_RS29250"
                     /old_locus_tag="V2L73_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(622..1431)
                     /locus_tag="V2L73_RS29255"
                     /old_locus_tag="V2L73_29255"
     CDS             complement(622..1431)
                     /locus_tag="V2L73_RS29255"
                     /old_locus_tag="V2L73_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(1424..2674)
                     /locus_tag="V2L73_RS29260"
                     /old_locus_tag="V2L73_29260"
     CDS             complement(1424..2674)
                     /locus_tag="V2L73_RS29260"
                     /old_locus_tag="V2L73_29260"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3490..3891)
                     /locus_tag="V2L73_RS29265"
                     /old_locus_tag="V2L73_29265"
     CDS             complement(3490..3891)
                     /locus_tag="V2L73_RS29265"
                     /old_locus_tag="V2L73_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032446877.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLSSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3891..4082)
                     /locus_tag="V2L73_RS29270"
                     /old_locus_tag="V2L73_29270"
     CDS             complement(3891..4082)
                     /locus_tag="V2L73_RS29270"
                     /old_locus_tag="V2L73_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032446876.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNEFDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          5..380
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.225"
                     /coverage="92.0"
                     /evalue="3.56e-168"
ORIGIN
        1 atggggaaga aggaaaggcc gcaaagcggc cgttttttca taggctccgc ccccctgaca
       61 agcatcacgg aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
      121 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
      181 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
      241 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
      301 tgcgccttgt ccggtaacta tcgtcttgag tccaacccga aaagacatgc aaaagcgcca
      361 ctggcagcag ccactggtaa tcggattcac ggagcggagt agagttatct acagagttct
      421 tgaagtggtg gcctgactac ggctacactg aaaggacagt tttggtatct gcgctccgtg
      481 aaagccagtt accttcggaa gaaagagttg gtagctctaa attccggcaa acgaaccacc
      541 gttggtagcg gtggtttttt tgtttacggc ttaggagatt acggctagaa cgaaaggatc
      601 tcaagaagat cctctcagaa attatacaga catgcggtag tttgagatgt cagagctatc
      661 taactctttt accggaacca gtttgtaatg cctttcctca atcaatttca cttgtccttc
      721 gtctatcttt agctcaaaca gtgcaatcaa atcgctcgaa acaaactgag cggaaatagc
      781 tctacatcta acatttgggt atttttgttc acaccacgct gtatcttgcg atgtttgcac
      841 cattgatatt tgatctgtcc caccttttgc ttgaactggt attatataat ggcaaccgta
      901 tttatctata cctacataaa gttcatcgat ctctatttga ccaattccct tcaccgtagt
      961 tcttaaatga ttctgtaaag aatatgtggt tatacctaga aatatatcta ttagccggtt
     1021 atatctcacc ttcgccagca atgcctgctc gtcgtctaaa gaataacctg taattatctc
     1081 cggggtattg tctggaattt ttattgccac catattagga tttggcaaaa tcctactaat
     1141 agttactaat ctaaatcgat actttgacct accagccagc tcaataatcc actccttacc
     1201 ttctggctga gtcaacagaa tactttctgg cataggcgtt ctaaatctga ttgaatatat
     1261 aacatcacca acattagatg ggagttctat acctaaatct tttgcagcac tgtttatatc
     1321 taacctttca aacaaaacct ctaagtcacc atcaacatat ttatcgaaaa atatcttttc
     1381 taccaactga tggtatctat ttttggacct ttctttttta tccttactca caaacaacct
     1441 ccttgttact attttttatt ctcttagcaa ttacatttga tgacacacca aacatagatg
     1501 cagcatcgga catagaaata gcaaccaaac tttcatcacc gagtttgatt ttcttcgttg
     1561 gtttttttgg cgtaacacca agaaccttca taatctgcaa gccaacactt ctagctagca
     1621 taggaggaac actattaccg acctgtctaa atccatgcca cttagtcaca tgaaatctga
     1681 accaatcagg atatgaatgc agtcttgctg cctctctgac cgtgatgacc ctaggataga
     1741 tcggatgtat aggacgagga gatgtaaaag cacctctgtc acttgctgtt cctgccctta
     1801 aagtattaca tagcccattc atcgagagct ttttaaacct gctgattggt tctaccgtgt
     1861 caggcaaagt ttcaacaaac cgccctctag aaatgtcaga gtgatctgtc agtaaagaac
     1921 acgttaattt gtctttatta aaaatccttg ggtagctaaa atcactagga tcttttccta
     1981 cgccatgcaa ataggatgag tatagtgatt gctcaccaaa tttagcataa acccaatctc
     2041 taccccatag ctcaggatat ttttccgcat caggaatatc cccaatagcg tccccaaccg
     2101 ttgttctaat ggataatggc tcagggtatg atggcagagc aattcctttc cttcccccca
     2161 ttaagaaaag acgacggcga tcttgaggaa ctccataatc agctgcgttt agcactttat
     2221 aatcgcttac aacgtcgtac ccgccatctt taaatgcatc cattatctct actaaaaact
     2281 gcttatgctt accaaccgtc aaccctttta cattttcaaa aacaaagtat ttaggcttta
     2341 gctcaagaac cagccgtaca tagtgataga ccagtttgtt tctatcatca tcaagcgcgc
     2401 gttttcctat taatgaaaag ccttggcagg gagcgcctcc gatcactaca tcaatctctt
     2461 tctcaccaat acctgaaact tccctaacgt attctccggt aacatcaaca acacttctac
     2521 atatcgtttt gcacatagga aagttatatt tgtgagcaga gcaatgtatt ggatctattt
     2581 caactgatgc cattatatca aagccagctt gctcgaaacc gagactcagc ccacccgctc
     2641 cagcaaataa atcaacagca atcggtctag tcattcttta cacccacgtt atataaaatc
     2701 aacatgatgc tatcagaaaa acgatcgctt taccgatcgt ttttctgatt tcgattgaac
     2761 ataacatctc ttgcccgcac cgctaaaacg gcctcagggc gtttctgacg ggaaaccggt
     2821 aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc
     2881 atgcatgagt atgcacgcaa gtgcgtagcg cgaaaattca cgccattcag ccatggtaag
     2941 tcctgtttcc ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg
     3001 gccgtgaagc gttgccggcg cagccggaaa accggttttc agcacgttca ggaatcgtgt
     3061 tcgtcgacgg ccggcaatca cgttatggca agtcgccgga cgactcacca cacgattccc
     3121 cggcgactct tcactagagt cgtacccaga gtcgccgagt ctaaaaaaca accaaacggg
     3181 gagtcgtgtc tagagtcggg tcgttattaa ctcattgttt ttatagtcct gagtcgttac
     3241 cgactctatg acgactcgcc ggggactcta tgacgagtcg tcatagagtc ggatctatgt
     3301 aaataaaatt taaaaaacga aatttcagga aagtaaacca ctgatattaa atacaaatct
     3361 gcaaaatttt aattttgcgt agtgtgtggg tatttttagt cgtgaggagc gttcagagat
     3421 ctggcgattt ttgggaaggt tcaggaactc cccgggatgg ccggagagga aaactcaagc
     3481 atggcaggat caggattttt tccagaattt ccaccatggc gccggggatt cagttaattt
     3541 tgcctggtga ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt
     3601 ggacgaaagt aaggtgttca ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc
     3661 actctggtca gcagaggctg tatcacgctg tccagagacc tgtccagaat ggtgttcacc
     3721 cttcggttgc tcaaagggac caaagacacg taaaagctct gaagtatcta ccatttttgc
     3781 attgcgctca ccgctacagg tggacaggtc acctgcttta attaacctgt gcagtgttgt
     3841 tctactctta cccgtcagtc gggcagcctc actaatagac actctggcca tcagttacca
     3901 tcctgtaatg catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg
     3961 agtttctcac aaaggtcggc ctcgacatcg agatcaaact cgttaagctt ctccagcagg
     4021 agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc
     4081 ataaatggcc tcagagcgtc agtgctcagt gtaaaaccag tctcttttcc gcttcctcgc
     4141 tcactcagtc gctacgctcc ggtcgttcgg ctgcggcgag cggcaggagc atgcgtataa
     4201 aaactcgaga agaccgagcg gtcaa
//
