LOCUS       NZ_CP143626             6850 bp    DNA     circular CON 06-FEB-2024
DEFINITION  Escherichia coli strain FJ_Ec_21 plasmid pZBEC21_04, complete
            sequence.
ACCESSION   NZ_CP143626
VERSION     NZ_CP143626.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39658208
            Assembly: GCF_036362825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Zhou,L., Wang,L., Li,Y. and He,B.
  TITLE     Isolating and genome-wide analysis of the E. coli from swine that
            had diarrhea symptoms
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Zhou,L., Wang,L., Li,Y. and He,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2024) Institute of Animal Husbandry and Veterinary
            Medicine, Fujian Academy of Agricultural Sciences, Pudang No.104,
            Fuzhou, Fujian 350013, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/05/2024 23:08:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,825
            CDSs (total)                      :: 4,703
            Genes (coding)                    :: 4,483
            CDSs (with protein)               :: 4,483
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 56 of 220
            Pseudo Genes (incomplete)         :: 165 of 220
            Pseudo Genes (internal stop)      :: 33 of 220
            Pseudo Genes (multiple problems)  :: 33 of 220
            Pseudo Genes (short protein)      :: 4 of 220
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143626.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FJ_Ec_21"
                     /isolation_source="Fece"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pZBEC21_04"
                     /geo_loc_name="China: Fujian"
                     /lat_lon="26.67 N 119.55 E"
                     /collection_date="2021-04"
                     /collected_by="Longbai Wang, Lunjiang Zhou, Yanling Li"
     gene            529..>609
                     /locus_tag="V2I96_RS00005"
                     /old_locus_tag="V2I96_00005"
                     /pseudo=""
     CDS             529..>609
                     /locus_tag="V2I96_RS00005"
                     /old_locus_tag="V2I96_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            837..1073
                     /locus_tag="V2I96_RS00010"
                     /old_locus_tag="V2I96_00010"
     CDS             837..1073
                     /locus_tag="V2I96_RS00010"
                     /old_locus_tag="V2I96_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000138111.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSLQRMFETTSQALE
                     RNGRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1165..1951
                     /locus_tag="V2I96_RS00015"
                     /old_locus_tag="V2I96_00015"
                     /pseudo=""
     CDS             1165..1951
                     /locus_tag="V2I96_RS00015"
                     /old_locus_tag="V2I96_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2020..3540)
                     /locus_tag="V2I96_RS00020"
                     /old_locus_tag="V2I96_00020"
     CDS             complement(2020..3540)
                     /locus_tag="V2I96_RS00020"
                     /old_locus_tag="V2I96_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="WP_219363009.1"
                     /translation="MFLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLVSMVISIEIS
                     SIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASFILKRLIKEKKIRNIMV
                     VVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKEIVTNDSYSHEI
                     INGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRGQLTS
                     SYQMTGGYYKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNSIDAPL
                     SLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVGTHLGLD
                     SPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYPSEDYKKT
                     FKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHNMFIGESLF
                     EKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKIELIKQLQLFGDR"
                     /name="LTA synthase family protein"
     gene            3833..4524
                     /locus_tag="V2I96_RS00025"
                     /old_locus_tag="V2I96_00025"
     CDS             join(3833..4082,4082..4524)
                     /locus_tag="V2I96_RS00025"
                     /old_locus_tag="V2I96_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_330766689.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARFIGVGLNTIFRHFKKLRTQSVTSRIQPGRDVIV
                     CAEMDEQWSYVGAKSRQRWLFYAYDRVRRTVVAHVFGERTMFTLERLLSLLSAFDVVVL
                     MTDGWPLYESRLKEKLHVISKRYTQRIERHNLRQHLARPGRKSLSFSKSVELHDKVIGH
                     YLNIKHYQ"
                     /name="IS1 family transposase"
     gene            4769..4918
                     /locus_tag="V2I96_RS00030"
                     /old_locus_tag="V2I96_00030"
     CDS             4769..4918
                     /locus_tag="V2I96_RS00030"
                     /old_locus_tag="V2I96_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162842177.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5303..5671
                     /locus_tag="V2I96_RS00035"
                     /old_locus_tag="V2I96_00035"
     CDS             5303..5671
                     /locus_tag="V2I96_RS00035"
                     /old_locus_tag="V2I96_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273383.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLAGVDSVSVSETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(6068..6385)
                     /locus_tag="V2I96_RS00040"
                     /old_locus_tag="V2I96_00040"
     CDS             complement(6068..6385)
                     /locus_tag="V2I96_RS00040"
                     /old_locus_tag="V2I96_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000718045.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     Typing          407..492
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="83.0"
                     /evalue="2.14e-32"
     Typing          complement(2..440)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.331"
                     /coverage="84.0"
                     /evalue="0.0"
     Typing          complement(6556..6850)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.178"
                     /coverage="99.0"
                     /evalue="5.41e-63"
ORIGIN
        1 accggataag gcgcagcagt cggactgaac gggggattcg tgcatacagt ccagcttgga
       61 gcgaactgcc ttcccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
      121 gcggaatgac accggtaaac cgaacggcag gaacaggaga gcgcacgagg gagccaccag
      181 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc caccactgat ttgagcgtca
      241 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct
      301 atcgtttccc tgttaagtat tttcctggca tcttccagga aatctccgcc ccgttcgtaa
      361 gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
      421 gaatatatcc tgtatcacat attctgctga cgcactggtg ccgccttttt tgtcctgcca
      481 catgaagcac tttctcacag tggtgcgaat tttttctcag cggtgcctat ggaaaagcga
      541 acgaaagaaa tcaaaatccg catgaccgag actgagcatc agcgtctgct tgaacggtgt
      601 gaccgtacag ccagcaactg gagagcctga aagcccagag cggcgcaggg gtagcgattt
      661 acaaggacga caagcagaag aacacctatc tggtggtact gccgaagaaa gcgcatggca
      721 ccgagaccta tacatcgaca gccggaaaca ccgtgatgaa gtacacctcg aaataacccc
      781 gtcagagccc acagaaaagc cacagcacgt ttttatcact gaggacaggc aaaaccatga
      841 ccgagatgga aaggcagctt ctgagcgcct tagaaagctt acaaacaagt tatgaacaac
      901 agcaacaggc gtggcaggac agttacagca gcttgcaacg catgttcgag actacctcac
      961 aggcactgga gcgcaacggc agggtatgtc tgaacttgag caatcaggtg aacgccttgc
     1021 acgaacaggt cgaacgcttg agcagcgatc tgagccgatt aacacgctcg taaagcagca
     1081 ggaacggcag ttacagcagg agaagcaagc aacccgctat cgaggtcagt ccatgtacca
     1141 cacaagccgt acttctcgga tgatttgcac tttggtgtgc gcatcgtagg gaaagaacgc
     1201 gctattctgg cgaaatacat ccagttcaat cagcctcacg ccatgttctg gctgggattt
     1261 gatgttgacc gcatcggcgc agccattgac tggagcgacc gcaacgctcc cgcacctacg
     1321 ctgaccatca ccaaccccga aaacggacac gcgcacctgc tttatgcgct ggaaacctct
     1381 atacgcacag cacctgacgg aaaaatgaaa ccgttacgct acgcagctgc cgtagagaat
     1441 gcgctacgta gaaaattggg agctgacacg ggatattcgg ggctaatttg caagaatccg
     1501 aaccacagtc actggaaaat tgccgccgat tatggtttag gccgtaactg cactctgttc
     1561 gacaaaaccc gcaagtgggc atatcgtgcc atccgtcagg ggtggcctga gtatgagcag
     1621 tggttacaag cctgctatga acgagcctgt gcctacaatt tacaattcgt atcaccatta
     1681 agtgaaaatg aagttcttgg aatatcaaaa agcatctcaa aatggacagc gaataatttt
     1741 tcagaggatc gttttattga gtatgtaaaa aatacgcacc gttcagaaat tcaaagaaag
     1801 agagggggta aaggaggaaa gctaagcaat tcaggaggac ggcctaaagg tagttttaat
     1861 attaattcct ataactttaa aaaaagaatg ggagtcttta ggtgttagtc gagccacatt
     1921 ctacaggcgg ctcaagaatg aaaaaaagta agttttatta tcattttaaa ataaccccac
     1981 gtggactaat aatcaacatg gggtaaaacc gccttttaat tatctatcac caaaaagttg
     2041 caattgtttt attaattcta ttttttcttt tcttttctcg atttccattt tcccattaca
     2101 cgttttcaca tacaggtcac ctattgcaga tgtacagttg agttcatttt tatttttttc
     2161 tttttcaaat agagactcac ctatgaacat gttgtgtgac tccttgatcc caagaatatc
     2221 tagtattgtt ggcgttaaag atagtgaatt taaaccgtgt gcattaatgt ctttgaactc
     2281 aacaccatta ctccatatta ttaatggaat ctgatcaaca aaaaaagtat tatcagtttt
     2341 gaatgttttt ttgtaatctt ctgatgggta acttgcatgg tcacttgtga ttatgacaat
     2401 tgtatctttc gataagcttg tcttattaaa atgagatata aaatcaccaa accaatggtc
     2461 taattgatga aagtgattta aatattcatt tctcccatca taaaacttta catcagggct
     2521 atctaagcca aggtgtgtac ctacagtata aacaccatag aagaaaggtt gtttagcctc
     2581 atttaatttt ttaaataata aatcgaaaga ctctttgtct gtgagttcac ctgacttttt
     2641 gttatagtcc tcccttccgt atatatgatt aaactttaaa gttgatagca ttaaactcaa
     2701 tggggcatct attgaattag atgcttgaaa atatgtatgg taaccatttt cctcaagtat
     2761 gattggtagt tttgttatgt aattgtaagt atttagttta tgttcgatct ctactttaga
     2821 tgcttggcca aggccagaat tatctttata gtaaccaccg gtcatttgat atgatgaggt
     2881 taactgtcct cttaaacctc taaatgtagc tgcggtatgg ttgtaatagt tgttaaatga
     2941 tattgatttt tttctaaatt catataaatt cggggttagc tccttagata ttaccaatga
     3001 agacatacct tcaataaata ttactaaaat attttttccg tttccattta ttatttcatg
     3061 tgagtaactg tcgtttgtta ctatttcttt tgtgtatttt gactttatat cacttatttc
     3121 atgtggtgac atattgtatt cttttgcttc cgaataaact tcatatgatg aagaaaaaaa
     3181 agcatttatt ggagatattg gtattaacaa tgataataaa aaaacaacaa ccattatatt
     3241 tcttattttt ttttctttta tcaatctttt caaaataaat gatgcaatca taaatgataa
     3301 gaacacaaga aaaggtagta gttttatttt ccagccaaca ctatttgtct ctgatatatt
     3361 tgctagtgca agtgtgggta tgtactcacc tattgatata atgctgctta tttcaataga
     3421 tataaccatg gatactaata gaagtaaaag cagaataatt gctctaaatg catcttttat
     3481 ttttatatta agcattataa tcataaccga gaacatttga ataattgaaa ttaaaaacat
     3541 ttataaaaac cttttttttt tgggggcgtt gttgaataaa tagatattat gctgtggatc
     3601 tacaaaaatg tcggtcttcc tcaacctaaa atcagtaagt taagtttaga ctcgggagct
     3661 tatcacaata ttgataagcg catttgacta aataagacat agaaaacagc ataattctcc
     3721 attattcaac aaatctggag atggattata cttttttaat taactgtctg ctagtgcggt
     3781 gatgcttcca acttactgat ttagtggatg acggtgcttt tgaggtgctc cggtggcttc
     3841 catttccatc agctgtccct cctgttccgc tactgacggc gttgtgcgta acggtaaaag
     3901 tactgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc agctacagtt
     3961 cacttacacc gcctctcaac ccggtacaca ccagaaaatc attgatatgg ccatgaatgg
     4021 cgtcggatgc cgggcaaccg cccgctttat tggcgttggc ctcaacacga ttttccgcca
     4081 tttaaaaaac tcaggacgca gtcggtaacc tcgcgcatac agccgggcag ggacgtcatc
     4141 gtctgcgcgg aaatggacga acagtggagc tacgtcgggg ctaaatcacg ccagcgctgg
     4201 ctgttttacg cgtatgacag ggtacgcagg actgtcgtgg cccacgtttt tggtgaacgc
     4261 acaatgttca cgctggagcg tcttctgagc ctgctatcgg cctttgacgt ggtggtattg
     4321 atgacggacg gatggccgct ttatgaatcc cgcctgaagg aaaagctgca cgttatcagc
     4381 aagcgatata cgcagcgaat tgagcggcat aatctgaggc agcatctggc aaggccggga
     4441 cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
     4501 ctaaacataa aacactatca gtaagttgga ttcattatcc tacctacctc ttggcaggaa
     4561 aaagcgctcg caaaaacggt gagaaaaaaa caagcctata tcagataaca gccctctcct
     4621 agacagggct gttactttgc cgtaaccaca gaatctgtca tactacacta ccgcctaagt
     4681 cagtatacac tccgctagcg ctacgtgact ggtttagggc tgcgccccga aacccgctaa
     4741 ccacctctca gctactggat atcaccccat gccatctgaa catgatgaac aactggattc
     4801 gactgatacg cagcttcccg gcgaatatga cctttttgat ttaatggcgc aggttactga
     4861 agagaacctg cacgccccgg tggattttgg gccgcctgtc ggccgggaag cgttatagct
     4921 ttttcgctta cccgcgtacg tggtaatacg ccggtattac atcttatttc tttattttca
     4981 tgtaattaca ggtggttgta atacgttgcg tattacataa ataaacgggg acttcagttt
     5041 ttacctttta attcatgcaa ttgcgatgaa aatcgcggtt gcgtatggtg tatgaccatt
     5101 taagggataa tgtaacctga cttttaaaga atgaatctat ctttggctgc gcatgagatc
     5161 gataaaaagc gttccacatc aggcggttca tggatacatg aaccggaaag gacaacaata
     5221 agacaattgt aagacatttt tttgcgtttc aatcttattt ttggactatt atgagacaaa
     5281 tttaagacat tatgggcggt atatggcgaa ttttaaccct ggtccaagaa cagccattgc
     5341 ggctgtcagg gattccaaga acgatataga aacctggaag aaaaatggta tccgtaggga
     5401 agagattgtg actgtgctga atgaacgtta cggcctgaat atctcactgg ttgcgtttga
     5461 taaggctctt tacagattga gaaaaagtac agatgaaccg tctcttgctg gtgtggattc
     5521 tgtgtctgtt tcagaaacga atttctctga agccagtggg cctgacaatg gtttttttct
     5581 gtccgggaat gacttcatgc gagcagaaga agaatctcaa agagtggttg agtcattcag
     5641 agatagcctt agcaggaaga aaggattctg attttgaatt ctaagtagtg aaatagtgga
     5701 tcctctactc gaaattgtcg gatacctgca attacctacc gtgccttacg gcttggtcaa
     5761 tgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaatgc
     5821 cggaaacagg acttaccctg gctgaatggg gtgaattttc gcgctatgca cttgcgtgca
     5881 tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg
     5941 ttgcctgttt taccggtttc ccgtcagaaa cgccctgaga gcctctcagg cggtgcacgc
     6001 aacggatgtt atggtaaatc cagctgattg gcaggtacaa aaaagccccg cagggctttt
     6061 ttgtacctta ttgatctgca cactttgcag ccttcagggc ttccagctga tttagccggt
     6121 tagctgcttt tttgcgggct tcgccttttg ccatgccatt tcctatccca aaatcaccca
     6181 ggaaacctaa tacggtgcgc ccgtcaaatt cgccggtttc ctcaatttct tgttgtacac
     6241 tgcgcgtttt ggcgatttcc agagctaccg ctttgcaatc cattgcggaa gattctggac
     6301 cggtgacgtt cgcggcctgt ggatattgct ttgttgcgca tcctgccatg attagcgctg
     6361 ccgataggat gaatattttt ttcaaaatta tccttattta ggcatagtta agagataccc
     6421 attttatcgg caggaaatga gggtttttta gttgaatttt gggaggggca aggttccata
     6481 ttggtcgagg attctggagc tgaccggcaa cggccagcgc ggctatgagc catgcggtgg
     6541 ttgagcatag aaaggatctt cttgagttcc tttttttcta cgcgtaatct cttgctctga
     6601 aaacgaaaaa accacctggg gaggtggttt ttcgaaggtt aagtcagttg gggaactgct
     6661 taacctggta actggcttca gcagagcgca gataccaaat actgtccttc cagtgtagcc
     6721 gtagttaggc caccacttca agaactctat agcaccacct acattaactc gctctgctaa
     6781 tcctgttacc agtggctgct gccagtggcg ctttgtcgtg tctttccggg ttggactcaa
     6841 gacgatagtt
//
