LOCUS       NZ_CP143506             3541 bp    DNA     circular CON 05-FEB-2024
DEFINITION  Klebsiella pneumoniae strain B18185 plasmid pB18185_5, complete
            sequence.
ACCESSION   NZ_CP143506
VERSION     NZ_CP143506.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39589781
            Assembly: GCF_036347655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/03/2024 23:10:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,773
            CDSs (total)                      :: 5,652
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 59 of 164
            Pseudo Genes (incomplete)         :: 100 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 18 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143506.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18185"
                     /isolation_source="Hospital sewage"
                     /db_xref="taxon:573"
                     /plasmid="pB18185_5"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            complement(join(3386..3541,1..246))
                     /locus_tag="V1666_RS28845"
                     /old_locus_tag="V1666_28845"
     CDS             complement(join(3386..3541,1..246))
                     /locus_tag="V1666_RS28845"
                     /old_locus_tag="V1666_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(246..437)
                     /locus_tag="V1666_RS28850"
                     /old_locus_tag="V1666_28850"
     CDS             complement(246..437)
                     /locus_tag="V1666_RS28850"
                     /old_locus_tag="V1666_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            851..964
                     /locus_tag="V1666_RS28855"
                     /old_locus_tag="V1666_28855"
     CDS             851..964
                     /locus_tag="V1666_RS28855"
                     /old_locus_tag="V1666_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072032591.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPKRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1262..2155
                     /locus_tag="V1666_RS28860"
                     /old_locus_tag="V1666_28860"
     CDS             1262..2155
                     /locus_tag="V1666_RS28860"
                     /old_locus_tag="V1666_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_330492969.1"
                     /translation="MELNLCWWNIGISPPVKGVKKDKAEAIRLAKHYIKKMSQEKTLDF
                     LGICEISEDEAIDFKPLADKLHLEYLNLSGTVGRIIMDVSIMYEASKLEYIDYKYIIKI
                     QPDDRTLRIAVRVVFKDAVTGNLITFFLSHWPSIRSADEKIREDAAESLRNNVNKILDD
                     HGIESQIICMGDYNTNPYSVAINEKLYATRDYHRIKNKRKLLFNPFWYLLSDKKTNNLG
                     TYHYKSGASNRWFVFDQMLFSSSFIYGGKDCLKLDMNSLDFHRILSDDNQCLDQRFFNI
                     FDHYPIFCRIYHDNQK"
                     /name="endonuclease/exonuclease/phosphatase family protein"
     gene            2139..2576
                     /locus_tag="V1666_RS28865"
                     /old_locus_tag="V1666_28865"
     CDS             2139..2576
                     /locus_tag="V1666_RS28865"
                     /old_locus_tag="V1666_28865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032419556.1"
                     /translation="MTTKNEEFQERLKASLKKANETLILKEKIESDISSILSMLKGITS
                     DSIDFDIFQNSSKIPKYKDCKKIVRIKKNSSMAYPKGFILFGFSINESTGYPVQVETED
                     EAAECTDVDNLKESIVYIIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     Typing          559..964
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.643"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 acgaatcacc tgttcactct ggtcagcaga ggctgtatca cgctgttcag agacctgtcc
       61 agaatggtgt tcacccttcg gttgttcaaa gggaccaaag acacgtaaaa gctctgaagt
      121 atctaccatt tttgcatttc gttcaccgct acaggtggac aggtcacctg ctttaattaa
      181 cctgtgcagt gttgttctac tcttacccgt cagtcgggca gcctcactaa tagacactct
      241 ggccatcagt taccatcctg taatgcatcc agtctcgagg acagtgtacg gaaaagatgc
      301 tccgcatcat cgtggagttt ctcacaaagg tcggcctcga catcaagatc aagctcgtta
      361 agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt cagcgctgtt
      421 ttctgttgtt tcgtcataag aagcctcaga gcgtcagtgc tcagtgtaag aacagtctct
      481 tttccgcttc ctcgctcact cagtcgctac gctccggtcg ttcggctgcg gcgagcggca
      541 ggagcatgcg tataaaagct cgagaagacc gagcggtcaa atggggaaga aggaaaggcc
      601 gcaaagcggc cgtttttcca taggctccgc ccccctgaca agcatcaccg aagcggacgc
      661 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgat
      721 ggctccctcg tgcgctctcc tgttcctgcc attcggttta ccggtgtcat tccgctgtta
      781 tggccgcgtt tttctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
      841 gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta
      901 tcatcttgag tccaacccga aaagacacga caaagcgcca ctggcagcag ccactggtaa
      961 ctgatttaga ggagttagtc ttgaagttat gggtcagtta aggctacact gaaagaacaa
     1021 gttttggtga ctgcgctcct ctactagcca gttacctagg ttcaaaagac ctgcaagtta
     1081 tttgaaccct tgaaaaaacc gccctgcaag gcggtttttt catttccagc caagaagata
     1141 acgccttaaa aggggtagcg caacaccatt tatcaacaaa cagataaata aaatgtttaa
     1201 cccccacatc aaaagcaata caataaaccc aatgaataca atgcattaag aaagaaacac
     1261 catggaattg aatttatgtt ggtggaatat tggaatatca ccacctgtaa aaggcgtgaa
     1321 gaaagacaag gctgaggcta ttagattggc caagcactat ataaaaaaaa tgtctcaaga
     1381 aaaaacattg gattttttgg gtatctgtga aatatcagaa gatgaggcta tagattttaa
     1441 acctttggct gataaactac atcttgaata cttgaattta tcagggactg ttggtcggat
     1501 aattatggat gtatccataa tgtacgaagc atcaaaactt gagtacatag attataaata
     1561 tataattaaa atacaaccag atgatagaac tttgcgtatt gctgtcagag ttgtttttaa
     1621 ggatgcagta acaggaaatc taataacatt ctttttatca cactggccat ccataagaag
     1681 cgcagatgaa aagattcgag aggacgctgc cgagtcactg cgcaacaatg tgaataaaat
     1741 actagatgac cacggcattg agtcccaaat aatatgcatg ggtgattaca acacaaaccc
     1801 ttactctgtt gcaataaacg aaaaacttta cgccactaga gattaccatc gaataaagaa
     1861 taaacgaaaa cttcttttta accctttttg gtatttactt tcagacaaaa agaccaataa
     1921 cttaggaact tatcattata aatcaggagc atcgaataga tggtttgttt ttgatcaaat
     1981 gttgttttca tcatcattta tatacggagg aaaagattgt cttaagctag acatgaacag
     2041 ccttgatttt catcgtatac ttagtgatga taaccaatgt ttagatcaaa gattttttaa
     2101 catttttgat cattacccaa tattttgtag gatttaccat gacaaccaaa aatgaagagt
     2161 tccaagaaag actaaaggca tcactaaaaa aagccaatga aacactaatt ttaaaagaaa
     2221 aaatagagtc agatatttca tcaattttgt caatgctaaa gggtataaca tccgactcaa
     2281 ttgactttga tatttttcag aatagttcga aaattcccaa atataaagat tgtaaaaaaa
     2341 ttgtcagaat aaagaaaaac tctagcatgg cataccctaa aggtttcatt ttatttggat
     2401 ttagcataaa tgagtctacc ggatatcctg tacaggtaga aacggaagac gaagccgcag
     2461 agtgtacaga tgtggataac ttaaaagaat caattgttta cataatagaa aaaagatcta
     2521 tagagataat gaaattaata tccgagcaac aagaagacga tgacattcct ttttaaaaag
     2581 gtagcatgta atgccattat caacggcact ttttcataga aggtgccata tatcttagaa
     2641 tcaactaatt taacataaga ccataattgt acgcaccgct aaaacggcct cagggcgttt
     2701 ctgacgggaa accggtaaaa caggcaacaa accacccgaa aacccgccag aaacgcgcag
     2761 gctctgtttt tacggcatgc atgagtatgc acgcaagtgc atagcgcgaa aattcacgcc
     2821 attcagccag ggtaagtcct gtttccggcg ttcttcccat ccgaaaacgg tgaacgggga
     2881 cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccg gttttcagca
     2941 cgttcaggaa tcgggttcgt cgacggccgg caatcacgtt atggcaagtc gccggacgac
     3001 tcaccacacg attcaccggc gactcttcac tagagtcgta cccagagtcg ccgcgtctaa
     3061 aaaacaacca aacggggagt cgtgtccaga gtcgggtcgt tattaactca ttgtttttat
     3121 agtcctgagt cgttaccgac tctatgacga ctcgccgggg actctatgac gagtcgtcat
     3181 agagtcggat ctatgtaaat aaaatttaaa aaacgaaatt tcaggaaagt aaacagctga
     3241 tattaaatac aaatctgcaa aattttaatt ttgcgtagtg tgtgggtatt tttagtcgtg
     3301 aggagcgttc agagatctgg cgatttttgg gaaggttcag gaactccccg ggatggccgg
     3361 agaggaaaac tcaagcatgg caggatcagg attttttcca gaatttccac catggtgccg
     3421 gggattccgt taattttgcc tggtgatttt caagaaggag catggcctgt ttcaggctgt
     3481 caatgtgcga atccttggac aaaagtaagg tattcaggtg ttcaatctcc tgtttcagat
     3541 g
//
