LOCUS       NZ_CP143505             4052 bp    DNA     circular CON 05-FEB-2024
DEFINITION  Klebsiella pneumoniae strain B18185 plasmid pB18185_4, complete
            sequence.
ACCESSION   NZ_CP143505
VERSION     NZ_CP143505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39589781
            Assembly: GCF_036347655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/03/2024 23:10:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,773
            CDSs (total)                      :: 5,652
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 59 of 164
            Pseudo Genes (incomplete)         :: 100 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 18 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18185"
                     /isolation_source="Hospital sewage"
                     /db_xref="taxon:573"
                     /plasmid="pB18185_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            726..1184
                     /locus_tag="V1666_RS28820"
                     /old_locus_tag="V1666_28820"
     CDS             726..1184
                     /locus_tag="V1666_RS28820"
                     /old_locus_tag="V1666_28820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            complement(1692..1904)
                     /locus_tag="V1666_RS28825"
                     /old_locus_tag="V1666_28825"
     CDS             complement(1692..1904)
                     /locus_tag="V1666_RS28825"
                     /old_locus_tag="V1666_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_330492968.1"
                     /translation="MTELEKQLLTALELLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1908..2393)
                     /locus_tag="V1666_RS28830"
                     /old_locus_tag="V1666_28830"
     CDS             complement(1908..2393)
                     /locus_tag="V1666_RS28830"
                     /old_locus_tag="V1666_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2431..3108)
                     /locus_tag="V1666_RS28835"
                     /old_locus_tag="V1666_28835"
                     /pseudo=""
     CDS             complement(<2431..3108)
                     /locus_tag="V1666_RS28835"
                     /old_locus_tag="V1666_28835"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3071..3412)
                     /locus_tag="V1666_RS28840"
                     /old_locus_tag="V1666_28840"
     CDS             complement(3071..3412)
                     /locus_tag="V1666_RS28840"
                     /old_locus_tag="V1666_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3500..3679)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          2..128
                     /name="rep_cluster_2406"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.916"
                     /coverage="81.0"
                     /evalue="1.5e-18"
     Typing          3656..4052
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.778"
                     /coverage="93.0"
                     /evalue="1.34e-133"
     Typing          complement(1577..3081)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtccgactac cacgctcttt tcggtaacta tcgtcttgag tccaacccga aaaggcacga
       61 caaatcgcca ttggcagtag ccactggtaa acaggatgtg cgagagatgt attcagagtt
      121 cttgaagtgg tggccttact gcggctacac tggaaggaca gctttggtga catgtctcgc
      181 acaagactgt taccacggtt agtaaatctg ccaggattaa ctaaccttcg aaaaaccacc
      241 ttccagggtg gttttttcgt ttacagagca aaagactacg ctcagaaaga gattatctca
      301 agaagatcat ctctttctct tactcaagtc aaagcaaaaa aacaccactc catcacaaac
      361 tcaggttata gtcggtatgc aactaataac actgtaattt ctgtgcaaaa tcgaggttat
      421 gcatatgcaa atcaacatca actgcaagag tatcccaaca tctttcgatg ttgtctgctc
      481 gaattgtgaa tggaccggcg tggctacatt agattatccg gaaaacgtaa aaaccctatc
      541 tgtcggagga gaaataaatt tcactcataa tgacgagacg ccaaaatgtc cacaatgtag
      601 cgttggatat atatatggta aaagtggcac ttataaacgt aatagtgcaa ctaaccatat
      661 ggacagaatc ggagattata aagagtaaat aatacgcatc acagcaatct aacgagggaa
      721 atataatgat tgaaaccaca ctacttgccg ttctcactgg cgcattaagc aatcttactt
      781 ctgatgcaat caaaggcatc atagggaagt tagcaagtaa acatcctgaa gttgaacaac
      841 aagcctcact gcttgaaaca ccattacctt ttgacgaagt tgataaatta ttttctcaga
      901 tgcagggggc cattcaggct gcagcagcca atggtgaaat tcagctagat ggagctgaat
      961 taaaagcatt gagcggaata aaatttgatc accaagccgg aactgttttg ataaatggat
     1021 ctcaagtaca agcgtcaatt ttagtcactg gtggaggtca acatgctact ggaaaaacga
     1081 tcatacaagg tgatacgaca atgactagcc agggtacaag tataaaaatg tcaggtggtg
     1141 ctcaaatagt aatgactggt ggagcccgaa tcattcagaa ctaatatgat ttaccataac
     1201 atctcttgcc cgcaccgctc cggacatgtt cgtcacaaac ggggcggcag tcaggtaaaa
     1261 ccggcaacaa accacccgat acaccgcccc gttcgtgaca gataaatttt acccggctgc
     1321 atggtcatgc agccagatgc atacggtctg atttcaggcc agaatctgga tgaacgcctt
     1381 aaaaacagga aaatgcttct ccagccagtc ccagatctcc agcgccatgt acaggcagtt
     1441 cagcaccgtc tcaatcacgt taaacagtct cataatccct ccgttgtata cgccgtgatg
     1501 ttttcatcac gaccgtattt agcgggaaat ttatgagccc ttcttcgtgg ggtttgtcac
     1561 tggtgaagcc tgtcagccca gcgtcggacc gttatagctc cgctgacggg aacgggcttc
     1621 cgccagctct ttttcgtgct gtcgctgctc gtatgcccgt tcggcggcca ccgccatttc
     1681 atgccgctgc actactggct caaacgctgt aactgcctgc tcaagttcgc cacctgctga
     1741 ctcaagcgcg tgacgcgctc gttcagcgcc gcgttctccc gttgcataag accagacatc
     1801 ttctgccatt ccgcgaaggc gctctcccac tcgtccagcc tttgcgagta gtcctgatgt
     1861 agcagctcta atgcggtcag caactgtttt tccagttccg tcatgggcta tttcctctcc
     1921 agtaccatcc agttcccttc ctccccgtaa gctcccgctt tcgggttcac tttgacgcag
     1981 gacagtttct tctccccgca gagcgacagc tgaacgccgt ggtttttctc cgacaacatg
     2041 gcctgcgtcc gctcctgcgc ccggatagtc tggtaattcc cggtgatctg ctgcccctgc
     2101 caccacagaa tgctcccgct catggcgatc agcagtaccg agaccagggt gatggtcagc
     2161 cacgtccggc cgaccatacg cagcacgctc aggcggttcg actgcatcgc cgcggtcata
     2221 ccctgctcgt gggcgctgat ggcgtcgctg attctcttcg cgcttgcgct cagttccgct
     2281 tttacagatt tctcgtgctc gctgaatgcg gctttcagca tctcgccggt actctgctgc
     2341 tgcgctttcg attgctgctc caagtccttc gccagcgcta aaagactgct cataaatggc
     2401 tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatac tgttttttgt
     2461 agtgcggacc acctcaaaac ctgccgctgt gagcgcacct gtgacgtcct cgcgagtttt
     2521 gagctccccg gatgaggcta aggccagcaa cccacgtgta atcgcctgag cggcttccag
     2581 cttcgtttcc ggcagtgcgg acggcgtaac cagtaaccgc cggttctccg gggcgtttgg
     2641 atcatgcagt cccagtctgc cgttcaccac ggtctgccag gcatcgatgc gcggccggtc
     2701 ggcgcgatcg taatacggct gcaggcgttt cccggtcagc agttcagtgt tggggatcag
     2761 gaaattcagc tccagccgcc ccttgtcggt gtgctccacc cacagaatgc tgtactggtc
     2821 tttatcgagc ccgggcatca gaacccgctc aaagctggcc atgatctgct cccgctgccc
     2881 gggcagcagt tcagcctccg cgaacgacag cacgccggag gtgtactttt tcgcatacgg
     2941 cgaggcatcg ataagctccc ggacttcctc cggttttccc tgcagcacgg tcgccccctc
     3001 gcgctgccgg tcttttccca gcaggtaatc caccggaccg gcaccaccac cgcgcccccg
     3061 gggatgaaat ttaacgatca tcatcccccc ctttttccag caccgcatgc cgcagccgct
     3121 ccagtcccgc gtctattgcc atcagggcgg ccacaatctg cacccgatca tgaccggcac
     3181 caacggcatt cactttccgg gcgatctggt tcaggttgtt acccataccg gcaagctggc
     3241 gcagcagcgc cggcgatatt gacggcagtc tgcaggcacg cgccggcttc tcatcgaggc
     3301 aggactgacg catccacgcc gccagctgcc tgccgtcgca ccgctcgagc agccgccggt
     3361 gttcgtcttc cgtcacccac atcgtgagca tcttgttgcg tttgtctgcc agagtagcct
     3421 cctgataaca ccgggacggg cgggaaatgt ttcccggtcg gcacggtgtt ggacaagccg
     3481 caggcgcgtc agtggctttt agcgggtttc ggggcgcagc cctgaaccag tcacgtagcg
     3541 ctagcggagt gtatactggc taaatatgtt acggcggagt ggtaatgtat gaaagtgctt
     3601 catgtgggaa ggggaaaatg tggctgacgg gcgtaagtgc aacatgctgg taaaggtgaa
     3661 atgacgcctc ctcgctcact cagtcgctgc gctcctgccg tgagactgcg gcgggcgtta
     3721 ccagctcaca gatgacggga tacgcaagca gcgctcaaat caggagaagc cgggaagagg
     3781 atgcggcgtt gccgtttttc cataggctcc gcccccctga cgagtatcat aaaatctgac
     3841 gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg
     3901 gtagctccct cgtgcgctct cctgttcctg ccattcggtt taccggtgtc attccgctgt
     3961 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     4021 aagctggact gtatgcacga tccccccgtt ca
//
