LOCUS       NZ_CP143504             5359 bp    DNA     circular CON 05-FEB-2024
DEFINITION  Klebsiella pneumoniae strain B18185 plasmid pB18185_3, complete
            sequence.
ACCESSION   NZ_CP143504
VERSION     NZ_CP143504.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39589781
            Assembly: GCF_036347655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/03/2024 23:10:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,773
            CDSs (total)                      :: 5,652
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 59 of 164
            Pseudo Genes (incomplete)         :: 100 of 164
            Pseudo Genes (internal stop)      :: 30 of 164
            Pseudo Genes (multiple problems)  :: 18 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143504.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18185"
                     /isolation_source="Hospital sewage"
                     /db_xref="taxon:573"
                     /plasmid="pB18185_3"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            complement(172..846)
                     /locus_tag="V1666_RS28785"
                     /old_locus_tag="V1666_28785"
     CDS             complement(172..846)
                     /locus_tag="V1666_RS28785"
                     /old_locus_tag="V1666_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_032419561.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEVEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISNLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            1194..1610
                     /locus_tag="V1666_RS28790"
                     /old_locus_tag="V1666_28790"
     CDS             1194..1610
                     /locus_tag="V1666_RS28790"
                     /old_locus_tag="V1666_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032419562.1"
                     /translation="MSDQKNNLDIQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            2028..2291
                     /locus_tag="V1666_RS28795"
                     /old_locus_tag="V1666_28795"
     CDS             2028..2291
                     /locus_tag="V1666_RS28795"
                     /old_locus_tag="V1666_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_054175658.1"
                     /translation="MAAYTSETEKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            complement(2841..3053)
                     /locus_tag="V1666_RS28800"
                     /old_locus_tag="V1666_28800"
     CDS             complement(2841..3053)
                     /locus_tag="V1666_RS28800"
                     /old_locus_tag="V1666_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3057..3542)
                     /locus_tag="V1666_RS28805"
                     /old_locus_tag="V1666_28805"
     CDS             complement(3057..3542)
                     /locus_tag="V1666_RS28805"
                     /old_locus_tag="V1666_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064147840.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYRTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<3580..4257)
                     /locus_tag="V1666_RS28810"
                     /old_locus_tag="V1666_28810"
                     /pseudo=""
     CDS             complement(<3580..4257)
                     /locus_tag="V1666_RS28810"
                     /old_locus_tag="V1666_28810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4220..4561)
                     /locus_tag="V1666_RS28815"
                     /old_locus_tag="V1666_28815"
     CDS             complement(4220..4561)
                     /locus_tag="V1666_RS28815"
                     /old_locus_tag="V1666_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4649..4829)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.978"
                     /coverage="83.0"
                     /evalue="2.08e-53"
     Typing          4779..5289
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.359"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2726..4230)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.622"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctggaaggac agttttggta tctgcgctcc gtgaaagcca gttaccttcg ggaaaaagag
       61 ttggtagctc ttgattccgg caaacgaacc accgttggta gcggtggttt ttttgtttgc
      121 agggcatgag attacgacga gagtaaaagg atcacaagaa gatccggctg tttaccagtg
      181 atttggcttc agtattggct tccgcctagt ggggacttta tctggcaata aagctaatct
      241 taaggcttca taggatacat atggcaagct ccaatcccct aaactagcca gattactaat
      301 cactcgacta gtatcctgtt cccttggcac tacgattcct atttttaact caggccgatc
      361 agcttttatc atttctattg gcggtatcat atctgagtcg ttagtgcaga gaaccaattg
      421 ttctacttca ccacgaatcg catcgcgata catttctaaa gccattctaa cgtctgtctg
      481 cttttcttct aacttccaga ccgttgttct atccgttttt gatggaggat gcccctcatt
      541 aaaggtcatt gcacattcct gacgctctga gtaataacct tttattatct gtagcttatc
      601 gccatagatc tgtactaatg ctcgatggta ggcttgctga gcttggtgcg ccaattcacc
      661 ccgagaagca aactttgcct tgatctcagc agtaaagaac ttcacctgaa tcaactgtga
      721 ttctcgtgct cttatcggaa gcagttgttc ttcaaagagc ttataaaggt ctaaccactt
      781 atatcccgaa cgcttaacca atccgtgata aaaattgaac ccatctacgt aaactattgt
      841 tctcatttta tttcgcgcat aaaaaaaccg cctctaaggg cggttttaac aacaaaaact
      901 aacgttagca aacagtttaa gttgtagtac ctcagggtta aaactaaccg agagggcggt
      961 ttcaagctcc tgaaggaaga cgcgtaactt aatcgctgcc cacctaaagg gaggtttaga
     1021 gcctcaaggg agctgcgtaa cttaatcgcg ctaccaagag gtgagtgtag attaagtata
     1081 gttaaatata atgatattat aagcgcactg gtgtcgtact aattatatga attaatttac
     1141 caacctgcta agctgaagtc ttctaagaag acttcagctt agcaggttgg tatatgtcag
     1201 atcaaaaaaa taatttagac attcaaaaac agacacttga gtctaaatgg gggaaggaag
     1261 cgttaagcat gggatggtca gccattccta catccctttt cttcctgcag ggcgcactca
     1321 atatgacccc cacaacattc aatgtgctac ttcatctaat tatccactgg tggaaaccct
     1381 atgattggcc acatccatct caagagagca tcgcggttag aatgggagtt tcatcaagaa
     1441 cagttcaacg ctcactccaa gaacttgaaa attacggatt gcttacaaaa attcgtacaa
     1501 gcaaggacaa ccctaagtac aaagggcgaa acatttacga tatgtctctt ttagttaagt
     1561 cactaaacat tcttacgcca gacctcaacg ccaaactaaa cgaaaagtaa ccaaaaattc
     1621 cgacagccaa agcgcgcaca tcactgtaca aaaggtgttc agcatcacat ttctagaaaa
     1681 tgacaacagc tccctattga aagctaattt ctaaactaaa catcagtatc gctacacaaa
     1741 cagcaatacg gacaaaacac aaaaagagga attagcatgt ctaaagcacc aattggtacg
     1801 actgcttcta ccggacaaaa gtgtcctgaa agtggtgtct ggaaagtcgt aggaacccct
     1861 tccactacag ccccgatagc aaaaggtaat gtaatgcctc cgtacgcagg aaaggctgta
     1921 acctggatgt taacccaata tgcttgatat ttagactggc aacgaaaagc cgccccagcg
     1981 ggcggctttt ctatctctgc cctataaatt cagaaaagga aagctaaatg gctgcatata
     2041 ccagcgaaac agagaagttt ttacaaagcg tcagtagcga tcaaacatgg tggaagtcac
     2101 gatataatcc aggagacagc gttccccatt ctggaattta tcgatgtacc gtctgcggaa
     2161 aagagatcac cagtaacggc agcgacccgt tcccgccgca aaaccatcat cagcacagtc
     2221 agcaacaagc aatcaaatgg caattgattg tcagaacgga cactaaaggt gaccgtttcg
     2281 gaatcaaatg attgagagcc ctccggggct ttttttgtac ctgaagctcg aagatattta
     2341 ccataacatc ccttgcccgc accgctccgg acaggttcgc cacaaacggg acggcagtca
     2401 ggtaaaaccg gcaacaaacc acccgataca ccgccccgtt cgtgacagat aaattttccc
     2461 cggctgcatg gtcatgcagc cagatgcata cagtctgatt tcaggccaga atccggatga
     2521 acgccttaaa aacaggaaaa tgcttctcca gccagtccca cagctcggtc gccagataca
     2581 ggcagttaag tacagcttca atgacgttaa gcaatttcat aattccccct aggtataggc
     2641 cgtgctcttt ttgatcacga acctatttag cgggagaaat aagagccctt cttcgtgcgg
     2701 cctgtcaccg gatgatactg ttcagcccag tgtcggaccg tcataactcc gctgacggga
     2761 acgggcttcc gccagctctt tttcgtgctg tcgctgctcg tatgcccgtt cggcggccac
     2821 cgccatttca tgccgctgca ctactggctc aaacgctgta actgcctgct caagttcgcc
     2881 acctgctgac tcaagcgcgt cacgcgctcg ttcagtgccg cgttctcccg ttgcataagc
     2941 ccagacatct tctgccattc cgcgaaggcg ctctcccact cgtccagcct ttgcgagtag
     3001 tcctgatgta gctgctctaa tgcggtcagc aactgttttt ccagttccgt catgggctat
     3061 ttcctctcca gtaccatcca gttcccgtcc tccccgtaag ctcccgcttt cgggttcact
     3121 ttgacgcagg acagtttctt ctccccgcag agcgacagct gcacgccgtg gttcttctcc
     3181 gacagcatgg cctgcgtccg ctcctgcgcc cggatagtcc ggtaattccc ggtgatctgc
     3241 tgcccctgcc accacagaat gctcccgctc gtggcgatca gcagtaccga gaccagggtg
     3301 atggtcagcc acgtccggcc gaccatacgc agcacgctca ggcggttcga ctgcatcgcc
     3361 gcggtcatac cctgctcgtg ggcgctgatg gcgtcgctga ttctcttcgc gcttgcgctc
     3421 agttccgctt ttacagattt ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta
     3481 ctctgctgct gcgctttcga ttgctgctcc aagtccttcg ccagcgctaa aagactgctc
     3541 ataaatggct cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact
     3601 gttttttgtg gtgcggacca cctcaaaacc tgccgctgtg agcgcccctg tgacgtcctc
     3661 gcgcgttttg agctccccgg atgaggctaa ggccagcaac ccccgtgtaa tcgcctgagc
     3721 ggcttccagc ttcgtttccg gcagtgcgga cggcgtgacc agtaaccgcc ggttctccgg
     3781 ggcgtttgga tcatgcagcc ccagtctgcc gttcaccacg gtctgccagg catcgatgcg
     3841 cggccggtcg gcgcgatcgt agtacggctg caggcgtttc ccggtcagca gttcagtgtt
     3901 ggggatcagg aaattcagct ccagcctccc cttgtcggta tgctccaccc acagaatgct
     3961 gtactggtct ttatcgagcc cgggcatcag aacccgctca aagctggcca tgatctgctc
     4021 ccgctgcccg ggcggcagtt cagcctccgc gaacgacagc acgccggagg tgtacttttt
     4081 cgcatacggc gaggcatcga taagctcccg gacttcctcc ggttttccct gcagcacggt
     4141 cgccccctcg cgctgccggt cttttcccag caggtaatcc accggaccgg caccaccacc
     4201 gcgcccccgg ggatgaaatt taacgatcat catccccccc tttttccagc accgcatgcc
     4261 gcagccgctc cagtcccgcg tctattgcca tcagggcggc cacaatctgc acccgatcat
     4321 gaccggcacc aacggcattc actttccggg cgatctggtt caggttgtta cccataccgg
     4381 caagctggcg cagcagcgcc ggcgatattg acggcagtct gcaggcacgc gccggcttct
     4441 catcgaggca ggactgacgc atccacgccg ccagctgcct gccgtcgcac cgctcgagca
     4501 gccgccggtg ttcgtcttcc gtcacccaca tcgtgagcat cttgttgcgt ttgtctgcca
     4561 gagtagcctc ctgataacac cgggacgggc gggaaatgtt tcccggtcgg cacggtgttg
     4621 gacaagccgc aggcgcgtca gtggctttta gcgggtttcg gggcgcagcc ctgaaccagt
     4681 cacgtagcgc tagcggagtg tatactggct caatatgtta cggaggagta acactttatg
     4741 aaagtgcttc atgtgacagg gggaaaatgc agctacaggg cgtacaggag aaaatggagg
     4801 ttacggtgat atgacgcctc ctcgctcact cggtcgcttc cgctcctgcc gttcggctgc
     4861 ggcgggcgtt acgcacacac ggataagagc aaaggcatat gacatgacaa gcagggcggg
     4921 aaaaggatgc ggcgctgccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     4981 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5041 cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     5101 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     5161 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttttcc
     5221 ggtaactatc gtcttgagtc caatccggaa agacacgcaa aaacgccact ggcagcagcc
     5281 actggtaaca ggattcacgg agcagagtag tagagtgctt ctacagagtt cttgaagtgg
     5341 tggcctgact gcggctaca
//
