LOCUS       NZ_CP143436             4150 bp    DNA     circular CON 02-FEB-2024
DEFINITION  Enterococcus lactis strain MElac1 plasmid pMElacP3, complete
            sequence.
ACCESSION   NZ_CP143436
VERSION     NZ_CP143436.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39280960
            Assembly: GCF_036324345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus lactis
  ORGANISM  Enterococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Jeon,C.O.
  TITLE     Metabolic features of key microbes in doenjang-meju, a key
            ingredient of Korean soybean paste, revealed by genome-centered
            metatranscriptomic analyses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Jeon,C.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2024) Life Science, Chung-Ang University, 84,
            Heukseok-Ro, Dongjak-Gu, Seoul 06974, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/01/2024 23:55:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,652
            CDSs (with protein)               :: 2,652
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 56 of 125
            Pseudo Genes (internal stop)      :: 41 of 125
            Pseudo Genes (multiple problems)  :: 16 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143436.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus lactis"
                     /mol_type="genomic DNA"
                     /strain="MElac1"
                     /isolation_source="doenjang-meju"
                     /db_xref="taxon:357441"
                     /plasmid="pMElacP3"
                     /geo_loc_name="South Korea: Seoul"
                     /collection_date="2022-12-27"
     gene            complement(join(3879..4150,1..91))
                     /locus_tag="VOF41_RS14285"
                     /old_locus_tag="VOF41_14285"
     CDS             complement(join(3879..4150,1..91))
                     /locus_tag="VOF41_RS14285"
                     /old_locus_tag="VOF41_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049143665.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFDDFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPNESDGLLGVIPSKKSQ
                     MSESKFTSMFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(95..433)
                     /locus_tag="VOF41_RS14290"
                     /old_locus_tag="VOF41_14290"
     CDS             complement(95..433)
                     /locus_tag="VOF41_RS14290"
                     /old_locus_tag="VOF41_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179115043.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYSE
                     MLERMMDVLNSVINADLAKSSVLTNEFVHRHLAQTTDEAEKEFWLKLMSHVMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            complement(412..831)
                     /locus_tag="VOF41_RS14295"
                     /old_locus_tag="VOF41_14295"
     CDS             complement(412..831)
                     /locus_tag="VOF41_RS14295"
                     /old_locus_tag="VOF41_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016173821.1"
                     /translation="MSDSIEKMTLKELADELGVTKNTVRNQLVNRGYDLSRYKENGIIM
                     LDKELIGVARSIYRSKTSQLTGNLPVDKAGNDFPLEKENEFLREQLVEKDKQISKLTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(828..1802)
                     /locus_tag="VOF41_RS14300"
                     /old_locus_tag="VOF41_14300"
     CDS             complement(828..1802)
                     /locus_tag="VOF41_RS14300"
                     /old_locus_tag="VOF41_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_330126979.1"
                     /translation="MSKRGTIINEWENLTALSNRRLNVGESVKMIKMSWSIDRITIVGK
                     LNENIYYHTSNDVLILNFEQLMRLNEGNGYLKSVSNNSWQLVDQHDENIAYIEILKWQE
                     GKGRIDFNPNKINQFLAGSMKNFIHDLFLDPHFSRADIACDMIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPKEIQSWWRLEMQLRRG
                     KATDWHAMVHETLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSAISRDLKYRLRNLL
                     KQESQNDELANHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2251..3657)
                     /locus_tag="VOF41_RS14305"
                     /old_locus_tag="VOF41_14305"
     CDS             complement(2251..3657)
                     /locus_tag="VOF41_RS14305"
                     /old_locus_tag="VOF41_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_330126978.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFQTTRADGSSTGISEQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERSMA
                     DFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     Typing          complement(832..1778)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.439"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2255..3657)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caatcaatcg ttttttctgt tgacgaagtt ttgcctgttc ttttttcaat ttttcttctt
       61 gtgctttaat atctgctaaa gtcatcccca taaactacat ccccttttct ttggaattaa
      121 taacggaaat ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt
      181 cagcttcatc agttgtttgc gctaaatgcc tatgaacaaa ttcattagtc aaaactgaag
      241 attttgctag atcagcattg ataaccgagt tgagaacatc catcattcgt tcaagcatct
      301 cactataatt atcttcatcc gaaagattca aataatcagc taaccgttca tacatcactt
      361 caattctatt gactgtttct tctctcaaat caattttatc catgtcacat ctcacttcca
      421 gaattgccac catttccgat tttttaattc ttgattttct agtaacaact gatcgttttt
      481 ttgttgctga agcaatgtca atttttgttg gtcacgaatc atttctgtta gtttagaaat
      541 ttgtttgtct ttttcaacta actgttcacg taaaaactca ttctcttttt ctaatggaaa
      601 atcattgcct gccttatcga ccggtaaatt accggtcaat tgactagttt ttgaccgata
      661 aattgaccga gcaacaccga tcaactcctt gtctaacatg ataataccat tttctttgta
      721 gcgagaaaga tcatacccac gattgaccag ttgattgcgc actgtatttt tcgttacacc
      781 cagctcatca gcaagttctt ttagggtcat tttttctatc gaatcactca ttcagcacct
      841 acttccgtta catcaagtcc ttgaagccat gtatctaatt ctcgtttcaa atcgtctgcc
      901 gattccgaaa atgtctctcg taaatgatta gctaactcat cattttggct ttcttgcttc
      961 aacaaatttc ttaatctata tttcaaatca cgactaatcg ctgaccactc ggaatggtca
     1021 tacaataaac cttttatcat tactctatcc aacgttttca catcttcagg aaaataatgc
     1081 ggactagcaa aactatccaa agtttcatgc accattgcat gccagtcagt tgcttttcct
     1141 ctacgcaact gcatttctaa ccgccaccag ctttgaattt ctttcggcac aattttacgc
     1201 ttagtctctt gctctaattt tttgttatac atccgaattt gtcgttcgct ggcacgacta
     1261 ccccagtaag cagtttctaa tttacctgac cttccataaa ttggtttaaa ggacacaggg
     1321 tcaaccactc gatattgggt aataaactca tctggcacat caatcatatc gcaagcaata
     1381 tctgcacgag aaaagtgtgg gtctaaaaat aagtcatgaa taaaattttt catagaacca
     1441 gctaaaaact gattgatttt attagggtta aagtcaattc gccctttccc ctcttgccat
     1501 ttcaaaatct caatataagc tatattttca tcgtgttggt ctaccaactg ccaactatta
     1561 ttgctaacag attttaaata gccatttcct tcattcaagc gcataagctg ttcaaaattt
     1621 aaaatcaata catcatttga agtgtgataa taaatgtttt cgttgagttt cccgacaatg
     1681 gttattcgat caatcgacca agacattttt atcattttta cgctctcacc gacatttaac
     1741 cgcctgttag atagggctgt taaattttcc cactcattta tgattgtgcc acgtttactc
     1801 aaattttgtt cctcctcaaa gaaaaagaga cttctggaaa gaattttcaa aagttcacgt
     1861 aactcttaaa aattcattac caaaaaatct ctagtcttga agaaatctaa aaatattcgt
     1921 ataatgatta agaagttacg ggactagttg ggatagtttc cgtaaaggtc gagaacgttg
     1981 gtagcgttgc tcgacttatt ttttttgctc tttttccagt tcatttgttt gctaactcaa
     2041 taatagcact tctctttagc tatgcaaata tccaaaaaat aaaaccgcac cctttgaaaa
     2101 gggcgccccc aaacttcgct aacgctaagt tggccaaaaa atggacttgc cattttttag
     2161 caaatgtcag cttaaaaggc tagtggttta gccgtcaact ccttatcctc ggcacaaacc
     2221 gcctccggta ccgttgactg cacatcgtaa ctaccactcg atctcatctt cagaacgctc
     2281 taatttatgc tgtgagacgt tttgacgata aaataaggtt ttctcctgct ctaatacttc
     2341 taattcctca gaaagcccca tatccttttc tgcaagacca aaacggatag acaaacgagt
     2401 gatcgatttt tgaaatgcgt tttttaatcg ctctaaacgc tggcgtaaag agcgattttc
     2461 ttctttcagt tttttatttt ccacttgtaa atctgctaat tgttttctac cagacaaatg
     2521 acgctcttct tccaatgcag ctacatactc atttaattct gtattttgct caagcatctg
     2581 taaatattgc tcactcatgt tatcagcatt tctcttggca tttgttgctc tcgtagctcg
     2641 ttgatacaaa tcaacaaaat cattcccaga taaatgatac gaaccatcag gaagcctctt
     2701 gaaactttct aaaggaacca tatctttttg gaaaatgacc gccatctctt tttttacatt
     2761 cagcatattt ttcttctctt cttcaatctc cgctctctca gaagccacca gagccttttc
     2821 tcgctcattt tcttctcttt gcctagaaat atacctcaac tcatcagaaa cgctctcacg
     2881 ctcttttacg gccgtttgac gcaattctga aatctcgctc atgatttctt tatattcgtg
     2941 aacatctttg attttgttag taccgactaa ctcacgctcc caaccaaaat ctgccatcga
     3001 acgttcaatt gactcaactt cctgattcct aaaattattc caaatcactc gaccattctc
     3061 tttatctgct cgtaaaccct gctgttgcaa ggctttattg aagcttggtt gcttctgaac
     3121 accacgtttg taaccttcag caaccggtac aatattcaag tgcaggtgcg gacttatttc
     3181 gtcattatga ataacagcat tataaacagc caaatttggg tttctctctt gaaattcctt
     3241 gtaatactga accaaaattt tattggcaat tactcgattt tgttcagctt gttgttctga
     3301 tatgccagta gaagaaccat ctgccctagt ggtttgaaaa tcatcaagag taccaatttg
     3361 cacgataaat tctcgctgtg gttccatttt tttatccttg cgaacctttg ctaaatagtc
     3421 ctcaattttt ctatcggctc ttttctgctt tgcattatat ttttctacgg cttcaccgaa
     3481 taattcttca tatttttcac gaatatttat ttgttccaaa tagatatttt catccgattt
     3541 tgtgaaatca atatgcttat gccagtcatt gattttttgg ctatcagtta actcacgatt
     3601 attatgagaa atgcttgtat tctttgttcc ctttttgaac gaaatactaa accccaagtt
     3661 aaacccctcc ttcgcgctgt cgctaataaa aagagtgtaa acctttctgt tactctttgt
     3721 caaagagtaa cgttttccta attgtgacat gactgtcaca attccaaacg ctctccgagg
     3781 gatttcgctg tcacaagtga caacgaaaaa aggagccgaa agcgtgtcac gctctcaact
     3841 ccttttacgc tttattttgc ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta
     3901 aacattgacg taaatttact ctcactcatc tgactttttt tactcggtat cactccgagt
     3961 aagccgtcag attcattggg ataatctcgt tcgcttggcg ctgatttttc atcagcaagc
     4021 cactcattat ccactgtttt ttcgtgttgc tgatcgtcag aaattgacga tagccaacgc
     4081 tcaaaatcat caaaagattc aaatccaaaa tgcttccgaa gtgccttacc catttcagca
     4141 ttttccttct
//
