LOCUS       NZ_CP143435             8759 bp    DNA     circular CON 02-FEB-2024
DEFINITION  Enterococcus lactis strain MElac1 plasmid pMElacP2, complete
            sequence.
ACCESSION   NZ_CP143435
VERSION     NZ_CP143435.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39280960
            Assembly: GCF_036324345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus lactis
  ORGANISM  Enterococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Jeon,C.O.
  TITLE     Metabolic features of key microbes in doenjang-meju, a key
            ingredient of Korean soybean paste, revealed by genome-centered
            metatranscriptomic analyses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Jeon,C.O.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2024) Life Science, Chung-Ang University, 84,
            Heukseok-Ro, Dongjak-Gu, Seoul 06974, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/01/2024 23:55:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,866
            CDSs (total)                      :: 2,777
            Genes (coding)                    :: 2,652
            CDSs (with protein)               :: 2,652
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 56 of 125
            Pseudo Genes (internal stop)      :: 41 of 125
            Pseudo Genes (multiple problems)  :: 16 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143435.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Enterococcus lactis"
                     /mol_type="genomic DNA"
                     /strain="MElac1"
                     /isolation_source="doenjang-meju"
                     /db_xref="taxon:357441"
                     /plasmid="pMElacP2"
                     /geo_loc_name="South Korea: Seoul"
                     /collection_date="2022-12-27"
     gene            1..933
                     /locus_tag="VOF41_RS14235"
                     /old_locus_tag="VOF41_14235"
     CDS             1..933
                     /locus_tag="VOF41_RS14235"
                     /old_locus_tag="VOF41_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_047927930.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEDDFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="VOF41_RS14240"
                     /old_locus_tag="VOF41_14240"
     CDS             950..1420
                     /locus_tag="VOF41_RS14240"
                     /old_locus_tag="VOF41_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_109114517.1"
                     /translation="MTDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKEINNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="VOF41_RS14245"
                     /old_locus_tag="VOF41_14245"
     CDS             1504..1956
                     /locus_tag="VOF41_RS14245"
                     /old_locus_tag="VOF41_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_002339186.1"
                     /translation="MTISKVIKQKRIEKQLTQEDIAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="VOF41_RS14250"
                     /old_locus_tag="VOF41_14250"
     CDS             2083..3264
                     /locus_tag="VOF41_RS14250"
                     /old_locus_tag="VOF41_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..3623
                     /locus_tag="VOF41_RS14255"
                     /old_locus_tag="VOF41_14255"
     CDS             3354..3623
                     /locus_tag="VOF41_RS14255"
                     /old_locus_tag="VOF41_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_047927326.1"
                     /translation="MKKTKLLVASLRLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            4430..4603
                     /locus_tag="VOF41_RS14260"
                     /old_locus_tag="VOF41_14260"
     CDS             4430..4603
                     /locus_tag="VOF41_RS14260"
                     /old_locus_tag="VOF41_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179189430.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDN"
                     /name="hypothetical protein"
     gene            5703..6083
                     /locus_tag="VOF41_RS14265"
                     /old_locus_tag="VOF41_14265"
     CDS             5703..6083
                     /locus_tag="VOF41_RS14265"
                     /old_locus_tag="VOF41_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6065..6979
                     /locus_tag="VOF41_RS14270"
                     /old_locus_tag="VOF41_14270"
     CDS             6065..6979
                     /locus_tag="VOF41_RS14270"
                     /old_locus_tag="VOF41_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002292460.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NKPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6939..7217
                     /locus_tag="VOF41_RS14275"
                     /old_locus_tag="VOF41_14275"
     CDS             6939..7217
                     /locus_tag="VOF41_RS14275"
                     /old_locus_tag="VOF41_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            7219..7908
                     /locus_tag="VOF41_RS14280"
                     /old_locus_tag="VOF41_14280"
     CDS             7219..7908
                     /locus_tag="VOF41_RS14280"
                     /old_locus_tag="VOF41_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_330126977.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYEQTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQTVTYERYENLFQQATNIQSPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6066..6976
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaaccata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccatta ggtactcatg aagatgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaacgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgacagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaatcaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactttatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatatagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taaatttatt
     2041 atgttatttt tatttataaa ttatgaaaaa ggacttttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatgcct taaagttaaa cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgcgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagt taaaatgtta gtaaatatta
     3541 gaggtaaaca aactatgcgt acttgttacc ttatgagttg gactgctagt agtagaacag
     3601 ccaaatatta ttactacatt tagtaaaaac aaaacattct ttctatacaa taaaatagcg
     3661 tatagagagg atgtttttaa tttgaaaagg agtggttatc tttatgaaaa atagatcatt
     3721 tgctatatta gtattatccg taattttatt agatatattg ttaataacat tagctttaaa
     3781 aactttaaat ttagtaacaa gttttgtcat tataccagta attataggaa tgtctattta
     3841 tttcatcaaa aaaaataaac aatttttttg aatgaaatta cgggcgaaaa taatcactca
     3901 aaattattaa taaaaacgaa tcaactactt tttctgaaga aacatacaac cgtaaacaac
     3961 aattgttgtt tacggttgta aattatcaaa ctatatggtg acttgttgct taaaataggt
     4021 tgaaatttcg tttatttttt ttgagaaatt ttacccacgt actacccatg tacctatcca
     4081 cgtattaccc atgtaccccc ccatgacaaa tgggcggagg ggcttgcggc gcttggtatt
     4141 aaaggcattc tccgcctccc aggtttctga ccattttctc tcaacgtggt aaaaacccca
     4201 cttatgagag aaaatggtca gaaacctaaa cgatcggcaa agaaaacata ccaagcgcca
     4261 caagcaaaaa aagttagagt aacttccaga aaagaaaaat tttttagaac aacttttgaa
     4321 aatttgattt tgatttatat ccgttcaatt taaacatttg ataaactgaa ggctagtatt
     4381 taaaatgtgt atacaagtgg tagcgttaaa aaaagtggag gtataaccta tgttaaaaaa
     4441 aactgtgttg gtactgggtt ctatttctct aggaatatta ggttttagcc ccatgctatt
     4501 atccgtaatt ggaaaaaaca attccaacag agaacttaaa ttaaattcaa attattacaa
     4561 cagtcctaac gaagaattgt ttgtaaaaga aacggataat taataaaaaa gactgctctt
     4621 ccgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcaacg cgcttatgaa
     4681 gcgctaccaa aaatttatca tattttatga aaaataacaa cccagtaaaa aatgactggt
     4741 aaaaattcac aaagaaacgg aaagaagcaa ctgcagaaaa tcaagcctta aaaagagctt
     4801 ttgacctaga ccaatcgaaa atcacaaagt gagcccctgt atcccttggt agagaggcta
     4861 cttggcatag tcgacattat gtctactagc tcttaatata acgtatttct ttatttagtt
     4921 gcacttatac ctatctaaca aaaatgtaat cattacctct gatatactcc tattgacaaa
     4981 actatccatt tggttctatc cttctttaca aagaaaatgt tttctatcca tttttttaga
     5041 tgtggtcata aatgataaag aaattcgtta aatttttaat cttttgtagt atgttttgta
     5101 tgggaaactt gtataagttt cccattttac ttgtgccaat gatataataa attaaaaatt
     5161 aagaagagga attttattat gttaatcctt tgcagtatat ttttaatagt ctttttgata
     5221 gtatttattt ttataataaa aatgatgaaa gaaaactccc aaaaagtaat attagctata
     5281 tatttaatcg ttgtattaag tattatatta tttttagtgt ggtttatttt tagtggaaat
     5341 gctatcaact acaaaccatt ttatggacca actaccattt agaaatcgat gaagagggac
     5401 ataaatgtag ttaaatcgtt gcaaagcaac aatttcaccg aaggtgaaat aagcgaaact
     5461 ttacaaaaag tggctagcaa atgctagcct tttttgtatt gtggagcgtg ggttttgagg
     5521 gggattcaag ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacggagg
     5581 tatttggcta gcaaatatag agcttgccaa acccttttag tgagctttac tcattgtacc
     5641 cttttaggta caatgatatt gcgaacgata cgtccgtagg acgtactgaa agggtgggac
     5701 ttatgagcga acaaaatcaa aatttggcta gcgatcattc taaaaaatat acctatcgat
     5761 ccgagccaaa acaaatcagt tttcgagtga gcgaatccga atttgcaaag ctgaagcagt
     5821 cagctgaagc tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca caaaacgcgc
     5881 gtctggtgac accgaaagtg gcgccagaca ttgcccaggc aatcgcgcgc gatttagcca
     5941 aagctggagg gaatatcaat caaattgcta agtggtgcaa cacacatcaa catgacgttg
     6001 ctcctggaga cgcccaacgc ttatccgaaa acctaaaaat catgcaaaag gagctacaaa
     6061 agatatggca acagttaaag taagtcgaac cacttcatgt agccgagcaa tcaattacgc
     6121 agaaccacga gcaaccgtca aaacaggaat aaactgtgac attgattatg ccaaaagtga
     6181 aatgaaacaa atacgcacgc tttatggaaa agacgatcgt gtacaagcac acttattgat
     6241 tcagtccttt cgaccaggag aaattaccgc tgaaaaagcc aaccaactag ggaaagaata
     6301 cgctgaaaaa atcgcaccag aataccaaat cgcgatttac acacacacag ataaagacca
     6361 tatccacaat cacattgtca ttaattcggt caatttagaa acaggaaaaa agtttcaagc
     6421 acatggtcaa gcattcctag ataagtgtta cgatatcaat gacgaaattt gtttaactca
     6481 tggtttaagt atcactgaac gaggaaaaaa agaagaaaaa cgaaccatgt ctgaaatcaa
     6541 attaaaagaa aaaaataaac cggtttggaa agacgaaatt cggtttgcga ttgatcaaac
     6601 catgaaaaac aagaaaacca gaacctatga ccaattttgt gattccctca aaacattcgg
     6661 cattcattgc ttcaatcgcg ggaaaaattt cacttacgaa ttgatccaca aaaagaaaaa
     6721 agtacgatcc aacagactag gaaaggacta cgaaaaggag acgattttac atgagttgga
     6781 cagacgagaa cgaacctata accaacagcg aaccaacgag ctacgaagag ctcgagctag
     6841 cattcgcgaa actgcagaag aagtacgaag agaacaacga actcttacgc ctgaacaacc
     6901 ggaaattaga ccggctcgaa gaaatcatga acaacacgtt gaaagaggaa cagaccacca
     6961 acaagaatta tcgctctaaa acgttaaaag acatagaaaa cgaaataaag aaacaacgtg
     7021 cttttctcgt tccttatacg caagaaagga tacaaactac gattgattta caagttaaaa
     7081 aactccatca agagctatta gaccagcgaa ataaggaaca gaaaaaggtg cttattcaac
     7141 gatcattgga ttttcttgcc cccttatgtt gcttagtcct gacactgttc gcattttggt
     7201 tagtatgggg aggctaaaat ggcattttat ttaccctatc tactcatttt tgttagtatt
     7261 tcaggaagta tttggctcat ctataaaatt ttccaaacaa gatattcatt aaaaggctct
     7321 aaaattcgat ttaagcgatt tttcttgctt ggctgtatat tttccttgat aatcgtctca
     7381 agcggcttat taggcgtttt agagggcaat aagcgcgttt ctagaagtat tttattagga
     7441 aatgtcactc aaaagtatga atcagcacgt aataaaaaga aaaaagagca agcattagca
     7501 caaaaaatgg aagagtttac cacttgttat gaggaaatga acgatatttt tgtgaatcaa
     7561 gaaaaacgtt tgacggataa aaacatggaa aaactcactc gtctttatca aaacttgcca
     7621 gaaaaacagc aaaaagaggt tcaagacaac tatgagcaaa ccaaaaaaga cgtgcaatat
     7681 gtaaaagata caaagataga agaagcctgt agtgatctat ttggagacac aaacccttgg
     7741 tttgccagtg aagaagaaaa aaaagaaaaa caacaaactg tcacatatga acgctacgaa
     7801 aacctgtttc aacaagcaac taacatacaa agtccaacga aaaaagaaac ggcattaaat
     7861 tatttaaaaa gtgtcaagga atggttagat cagcaacaac aaaattgaat ttgatttaaa
     7921 atcatgttaa actaaacatg caaataggta atcatgagat acacgaaagc cctagagaag
     7981 tcggattctc tagggctttt tttttgggct gatgtcgcct tagttgattt tacgtcttgt
     8041 ttcggttatt cagccaataa ctgaataaag agtttagaat ctcaacaaga agggcgcaaa
     8101 taaagttggt aatcacaaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
     8161 agttattata ccctacaaaa ttaaatctgt gtggcgattt taagtatctc ttattttagg
     8221 gttgttaggg cttgccctga ccgcctgtag ggcgctttaa attccagata aaggtttttt
     8281 actaagactg tcttaaaacg gcttagacaa gctcttatag cctcaaccct cctttttgaa
     8341 caatggtttt agcaaaggag ctggaggcga ctgcgcaggt ataatgtgag agcgtagcga
     8401 tcacaccttt ttggcgaccg tagggagcga ggttttgaat tagcagttta gaaaagttcg
     8461 cgaacctgtt taaagaaaaa agaattgttt tttaggtggg aaattttgtc tccctctttt
     8521 ataatctttt tttaaacctc tttttaaacc tctttaccgt cggctgtgtc cttactcccc
     8581 caagggatac aaaattttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     8641 ttataggcat gaaattatct gtttataggc atgaaattat ctgttttagg tattgttttt
     8701 agacataata tgtcctaaaa ttatgtcaat aaaatcattt agactggagt aacaaataa
//
