LOCUS       NZ_CP143304             5596 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CARKW-3 plasmid pCARKWE, complete
            sequence.
ACCESSION   NZ_CP143304
VERSION     NZ_CP143304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39526083
            Assembly: GCF_036321465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhou,Y., Chen,Q. and Leng,Y.
  TITLE     Klebsiella pneumoniae CARKW-3,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhou,Y., Chen,Q. and Leng,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2024) School of Basic Medical Sciences, Southwest
            Medical University, Longmatan District, Luzhou, Sichuan 646607,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:12:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,005
            CDSs (total)                      :: 5,880
            Genes (coding)                    :: 5,688
            CDSs (with protein)               :: 5,688
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 64 of 192
            Pseudo Genes (incomplete)         :: 137 of 192
            Pseudo Genes (internal stop)      :: 22 of 192
            Pseudo Genes (multiple problems)  :: 31 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CARKW-3"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCARKWE"
                     /geo_loc_name="China: Wuxi"
                     /collection_date="2023"
     gene            12..521
                     /locus_tag="V0R31_RS29975"
                     /old_locus_tag="V0R31_29975"
     CDS             12..521
                     /locus_tag="V0R31_RS29975"
                     /old_locus_tag="V0R31_29975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            528..740
                     /locus_tag="V0R31_RS29980"
                     /old_locus_tag="V0R31_29980"
     CDS             528..740
                     /locus_tag="V0R31_RS29980"
                     /old_locus_tag="V0R31_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1407..1631
                     /locus_tag="V0R31_RS29985"
                     /old_locus_tag="V0R31_29985"
     CDS             1407..1631
                     /locus_tag="V0R31_RS29985"
                     /old_locus_tag="V0R31_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1615..1881
                     /locus_tag="V0R31_RS29990"
                     /old_locus_tag="V0R31_29990"
     CDS             1615..1881
                     /locus_tag="V0R31_RS29990"
                     /old_locus_tag="V0R31_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1951..2346)
                     /locus_tag="V0R31_RS29995"
                     /old_locus_tag="V0R31_29995"
     CDS             complement(1951..2346)
                     /locus_tag="V0R31_RS29995"
                     /old_locus_tag="V0R31_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_329808112.1"
                     /translation="MKERIVKCVIPRFSTMYWLITFLAFIFPGRSFADGYTAYYRCNGT
                     QEASLTVKDNKLSMVVGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDV
                     NNMTLLVKNNGYIIVKTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2367..2912)
                     /locus_tag="V0R31_RS30000"
                     /old_locus_tag="V0R31_30000"
     CDS             complement(2367..2912)
                     /locus_tag="V0R31_RS30000"
                     /old_locus_tag="V0R31_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2938..3264)
                     /locus_tag="V0R31_RS30005"
                     /old_locus_tag="V0R31_30005"
     CDS             complement(2938..3264)
                     /locus_tag="V0R31_RS30005"
                     /old_locus_tag="V0R31_30005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3622..3780)
                     /locus_tag="V0R31_RS30010"
                     /old_locus_tag="V0R31_30010"
     CDS             complement(3622..3780)
                     /locus_tag="V0R31_RS30010"
                     /old_locus_tag="V0R31_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4420..4596
                     /locus_tag="V0R31_RS30015"
                     /old_locus_tag="V0R31_30015"
     CDS             4420..4596
                     /locus_tag="V0R31_RS30015"
                     /old_locus_tag="V0R31_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4586..4930
                     /locus_tag="V0R31_RS30020"
                     /old_locus_tag="V0R31_30020"
     CDS             4586..4930
                     /locus_tag="V0R31_RS30020"
                     /old_locus_tag="V0R31_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5112..5357)
                     /locus_tag="V0R31_RS30025"
                     /old_locus_tag="V0R31_30025"
     CDS             complement(5112..5357)
                     /locus_tag="V0R31_RS30025"
                     /old_locus_tag="V0R31_30025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4255..4334
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3672..4069)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..856
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.649"
                     /coverage="99.0"
                     /evalue="2.64e-162"
     Typing          4921..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.7900000000000002e-144"
ORIGIN
        1 gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag aaatcgaaag
       61 cgcagcagca gagtaccggc gaaatgctga aagccgcatt cagcgagcac gagaagtctg
      121 tcagagcgga actgagcgaa agcgggaaga gaatcagcgc cgccatccgc gcccacgagc
      181 agggtatgag cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg gtcgggcgga
      241 catggctgac catcacgatg gtgtcaggac tgctgttcgc cagcctgagc ggggtgttgt
      301 ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat cgaccggcag aacgcagcgc
      361 tgtcgaagct gaacgcgaag acctggggcg tgacgtacct ggaagacagc aacggacgtt
      421 ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg
      481 ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg
      541 aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg
      601 agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg
      661 cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac
      721 tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag aacgggcgca
      781 cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc gaagctatga
      841 cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat ttacgctgaa
      901 accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta tctgtcgcga
      961 acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc ccgttcgcgg
     1021 cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga
     1081 tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga tttgctgggt
     1141 ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt atcaggttcc
     1201 cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt tatcctttca
     1261 atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat tatcaagagg
     1321 ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga tatacatgta
     1381 tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat gagattgagt
     1441 cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag
     1501 caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa actgctgcac
     1561 gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc
     1621 tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga caaacagaac
     1681 gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga tcctcgccag
     1741 tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt gggagattat
     1801 cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac gattggacat
     1861 cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg acaacaggta
     1921 ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag ttaaccagca
     1981 ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat tgttaacgtc
     2041 aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc tctgcattac
     2101 tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa acttagaaag
     2161 acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct gagtaccatt
     2221 acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata taaatgctag
     2281 gaacgtaatt aaccaataca tagttgagaa tcttggtatt acacatttca ctatcctctc
     2341 tttcattttt cgttcaccat actttgttat ctgattttta atttttctat tttataaacc
     2401 aagtctctac caaaaaggcg aaaattatcc tcaaataata ataattgttt aagaacatcc
     2461 atgaaagcag ctgcttcacc ctcagttatg ggagctactc tatataacat ataacctgag
     2521 ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg attatcattt
     2581 ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt gcgtcctatt
     2641 gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc tttgtttatg
     2701 cctgcttgag tgattttcag agactctaca ccgtgtatgt tgtatgagat aaaatgtaat
     2761 aaattaagat gcaatgcaat taatccattt ttgtttacat ctgagtattt ggataaaaaa
     2821 cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgc cttgtatatt
     2881 tccttctccg ttaaataccg tttaaaaaac ataattctcc gcctccgtat agcgtgccta
     2941 ttttatttcg tcgtaaaact tagctctgaa atattccttt agaaccatca tgtcattaat
     3001 aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag ggagcggtaa
     3061 ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc attcaggctt
     3121 ttcgccaatg cgttcttcaa ctgaaagtga acctgataat ctctggttaa taagatcact
     3181 tagactcatt ttggttttat tcgaaagccg ttgtaaggat tgctttaatc ctatatctag
     3241 catgatattt gttctttctc tcattacccc cccttgctat tgcgtttttt gttgcgcaca
     3301 aagctaccat tgcgcattaa gaaacgcaat aaaaaaggag ggagggagtg gtttgtacat
     3361 gatgtgcagt gcgctgaggg catcagtggc gggtgttttt ccgtgggatg gatggagctc
     3421 cggggataac agaactgacg atcatttaaa ggatcttctt gagatccttt ttttctgcgc
     3481 gtaatctctt gcactgtaaa cgaaaaaacc gcctggggag gcggtttgat cgaaggttaa
     3541 gtcagttggg gaactgctta accgggtaac tgggtatacg agaccgcaga taccaaaact
     3601 gttctttcag tgtagccgca gttaggcacc cacttcaaga cctctcaata tctctctcgt
     3661 atatatccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtccgt ccgggttgga
     3721 ctcaagatga tagttaccgg aaagggcgca gcagtcgggc tgaacggggg gttcgtgcat
     3781 acagcccagc ttggagcgaa cgacctaaac cgagccgaga taccagcagc gtgagctatg
     3841 agaaagcgcc acgcttcccg aaagggaaaa aggcggaaca ggtatccggt aaacggcagg
     3901 gtcggaacag gagagcgcac gagggagccg ccggagagaa atcaccggca tctttaagtc
     3961 ctgtcgggtt tcgtcctcct ctgacttgag cgtccatttc tgtgatgctc gtcaggggag
     4021 gcggagccta tggaaaaacg gcaacgccgc atccttttct ccggcccttt ctgatttgag
     4081 cgcctttgct ttatcccgtt atccgtgagt cggtcacgcc cgccgcagcc gaacggcagg
     4141 agcgcagcta ccgagtgagc gaggaggcgt catttcatct gtccgtgcat ggtattgcgg
     4201 acgctcctga gccacatatc acacctgcca catgaggcac tttcatgaat cgtgctctca
     4261 tgccaacata gtaagccagt atacacagcg taaacgctgt gactggttca gggctgcgcc
     4321 ccgaaacccg ctaacaccac tgacgcgctg gcgcttgtcc aacgccggag cgacccggaa
     4381 aacttttccg tgccgtccgg cgtttgcgaa ggagggataa tgcagaagcc gaagaagaag
     4441 ccgctcgtcg taaaaaaaag atcctggcat ccggcagtga tggcaaatct gatgcgtcag
     4501 gcacaggagg aagattaccg ccgggagcag atggcagagc ttgagcgttc agcccggtca
     4561 cattttgcgg atatcggagg agaggatggc ggttaagcgt acccgttttt taggtatccg
     4621 ggtgaccgat ggggagtacc agcagctgct ggagcgctgc aatggaaggc aactggcggt
     4681 gtggatgcgg gaaacctgtc tcgatacgcg cccggcgcgg tcgttgcggc tgccgtccat
     4741 cgatccggtt ttgctgcgcc agcttgccgg gatggggaac aacctcaacc agatcgcccg
     4801 gaaaattaac ggcggccagt ggtcaggcgc tgaccgggtt caggtggtgg ccgcgctgat
     4861 ggccattgat gccgaactcg agcggctgcg gcatgccgtg ctggaaaagg gggcagacga
     4921 tgatcgttaa gtttcatccg cgagggcgag gcggcggtgc cgggccggtg gattatctgc
     4981 tggggaaaga ccgccagcgc gacggggcca tcgtcctgca gggaaagccg gaggaggtcc
     5041 gggagcttat cgacgcctcg ccctacgcca aaaagtacac ctccggcgtc ctgtcttttg
     5101 ctgaacagga tttaccgccc ggccagcgtg aaaagctgat ggcgagcttc gagcgggttc
     5161 tgatgcccgg actcgataaa gaccagtaca gcgtgctgtg ggttgaacac cgggacaagg
     5221 ggcggctgga actgaatttt ctggtaccga acacggaact gctgaccggc aggcgtctcc
     5281 agccgtacta cgaccgggca gaccgtccgc gcatcgatgc gtggcagacc gtggtgaacg
     5341 ggcgactggg gctgcacgac ccgaacgcac cggagaaccg gcgcgcgctg gtgacgccat
     5401 cttcgttacc caaaacgaag caggaagccg cagaggctat tacgcggggc ttactggccc
     5461 tcgcctcgtc aggggagctt aaaacgcgtc aggacgtcac tgaggcgctg gaaagcgcag
     5521 gttttgaggt cgtgcgcacc acaaagagca gcatcagcat tgccgacccg gacggagggc
     5581 gaaacatccg acttaa
//
