LOCUS       NZ_CP143303            11972 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Klebsiella pneumoniae strain CARKW-3 plasmid pCARKWD, complete
            sequence.
ACCESSION   NZ_CP143303
VERSION     NZ_CP143303.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39526083
            Assembly: GCF_036321465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Zhou,Y., Chen,Q. and Leng,Y.
  TITLE     Klebsiella pneumoniae CARKW-3,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Zhou,Y., Chen,Q. and Leng,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2024) School of Basic Medical Sciences, Southwest
            Medical University, Longmatan District, Luzhou, Sichuan 646607,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SOAPdenovo v. 2.04; unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:12:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,005
            CDSs (total)                      :: 5,880
            Genes (coding)                    :: 5,688
            CDSs (with protein)               :: 5,688
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 64 of 192
            Pseudo Genes (incomplete)         :: 137 of 192
            Pseudo Genes (internal stop)      :: 22 of 192
            Pseudo Genes (multiple problems)  :: 31 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143303.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CARKW-3"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCARKWD"
                     /geo_loc_name="China: Wuxi"
                     /collection_date="2023"
     gene            complement(join(11265..11972,1..24))
                     /gene="mobC"
                     /locus_tag="V0R31_RS29905"
                     /old_locus_tag="V0R31_29905"
     CDS             complement(join(11265..11972,1..24))
                     /gene="mobC"
                     /locus_tag="V0R31_RS29905"
                     /old_locus_tag="V0R31_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(24..1988)
                     /locus_tag="V0R31_RS29910"
                     /old_locus_tag="V0R31_29910"
     CDS             complement(24..1988)
                     /locus_tag="V0R31_RS29910"
                     /old_locus_tag="V0R31_29910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3474..3623)
                     /locus_tag="V0R31_RS29915"
                     /old_locus_tag="V0R31_29915"
     CDS             complement(3474..3623)
                     /locus_tag="V0R31_RS29915"
                     /old_locus_tag="V0R31_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3708..3965)
                     /locus_tag="V0R31_RS29920"
                     /old_locus_tag="V0R31_29920"
     CDS             complement(3708..3965)
                     /locus_tag="V0R31_RS29920"
                     /old_locus_tag="V0R31_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3975..5660)
                     /locus_tag="V0R31_RS29925"
                     /old_locus_tag="V0R31_29925"
     CDS             complement(3975..5660)
                     /locus_tag="V0R31_RS29925"
                     /old_locus_tag="V0R31_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5765..7252)
                     /gene="ltrA"
                     /locus_tag="V0R31_RS29930"
                     /old_locus_tag="V0R31_29930"
     CDS             complement(5765..7252)
                     /gene="ltrA"
                     /locus_tag="V0R31_RS29930"
                     /old_locus_tag="V0R31_29930"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7897..8142
                     /locus_tag="V0R31_RS29935"
                     /old_locus_tag="V0R31_29935"
     CDS             7897..8142
                     /locus_tag="V0R31_RS29935"
                     /old_locus_tag="V0R31_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8416..8787
                     /gene="tolA"
                     /locus_tag="V0R31_RS29940"
                     /old_locus_tag="V0R31_29940"
     CDS             8416..8787
                     /gene="tolA"
                     /locus_tag="V0R31_RS29940"
                     /old_locus_tag="V0R31_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8784..9149
                     /locus_tag="V0R31_RS29945"
                     /old_locus_tag="V0R31_29945"
     CDS             8784..9149
                     /locus_tag="V0R31_RS29945"
                     /old_locus_tag="V0R31_29945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9324..9602
                     /locus_tag="V0R31_RS29950"
                     /old_locus_tag="V0R31_29950"
     CDS             9324..9602
                     /locus_tag="V0R31_RS29950"
                     /old_locus_tag="V0R31_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9590..9904
                     /locus_tag="V0R31_RS29955"
                     /old_locus_tag="V0R31_29955"
     CDS             9590..9904
                     /locus_tag="V0R31_RS29955"
                     /old_locus_tag="V0R31_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10068..10496
                     /locus_tag="V0R31_RS29960"
                     /old_locus_tag="V0R31_29960"
     CDS             10068..10496
                     /locus_tag="V0R31_RS29960"
                     /old_locus_tag="V0R31_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10522..10701
                     /locus_tag="V0R31_RS29965"
                     /old_locus_tag="V0R31_29965"
     CDS             10522..10701
                     /locus_tag="V0R31_RS29965"
                     /old_locus_tag="V0R31_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10728..11258)
                     /locus_tag="V0R31_RS29970"
                     /old_locus_tag="V0R31_29970"
     CDS             complement(10728..11258)
                     /locus_tag="V0R31_RS29970"
                     /old_locus_tag="V0R31_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10660
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2791..3371
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.774"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11269..11972)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.043"
                     /coverage="97.0"
                     /evalue="3.82e-136"
ORIGIN
        1 gacgttgtac tgctccagtg tcatcagtaa agccccgcaa aatcatcatc cgcttttggt
       61 ccgccgccgt cggccggagg caggtactcc gccagcgggt ccgttttcgc cagcaccggg
      121 aaggttttca gcgaaatttc ccggcgctca acccgcagcg ggctgctgaa cgccagccgc
      181 gccacgccca gcccggtcat caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg
      241 tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc cgctgatgtg ctccacgttc
      301 cccgcctccc ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctggccc
      361 gccgcccact gcgcggactc ctggctggcc gcgcgataga accagcggat cggcgtgtta
      421 tccagtaccg tggttttcac aaaatcagcg ggcagatcct gcccgcactg accgaagtcg
      481 cccagcgact ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg
      541 ttcagcacgt acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc
      601 cacctccgga actcatcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc
      661 tgcacccgga tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg
      721 ttgattatcc ccgccacatc gataccgctg tccgtctgca ggacgctcag gtcggccacg
      781 ttctccagct tggtgatcag ccccttcacg tcctttttca gggcctccgg caacgcgtac
      841 gccgccgcca gcacctgctg aatgtttgcc ccctgcggga actgctccgc aatcagcttc
      901 gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc actcaggtta
      961 aacccggcaa ccagcagatt cttcagggcg tactggtcga gatcgcggaa cagattcagc
     1021 tgcgccggtt tccccggccg cagatccagc agggtgaagt ccgggcagtg cgcccggaag
     1081 acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg
     1141 cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct tgccgctccc gggcaggccg
     1201 aggatctgaa tattggtctt gcgccaggta tcacgaggga ccagcaccgg ctggccctgc
     1261 tcatccttac ccagaaacac cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc
     1321 agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc ggacgtctgt catttctccg
     1381 gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg
     1441 gccgcaaaat gaccatcaag cagcaggaac gccagcagcc gcgccagaag gacgccggcg
     1501 acaagccctc cggcacacca ggctatcccg aacaggttag ccggctgcag caggtaataa
     1561 tctgcgttcg catagaggtc cgggcgcagg acggtttctc cccagctctc gataaacacg
     1621 gcgacaacca gcccccccag aagggccaga ccggcagcgc cggtcagcca gtacgcgtag
     1681 ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg aatccagcga ggccagcacc
     1741 gaaaatggca cagtccggtt attatcgcgc ttatagcgat cggcatactc ccgccgcgcc
     1801 ttatggctca gccccacaaa acgcattcgg ccggcccaga gttcagccag accgaaaaac
     1861 aggccgataa ccagaaaaaa accggccaga ctgtaatact cccggtacag ccagctgacc
     1921 cacgggtaaa tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta
     1981 ttaaacatgc aatctctctc cttcagacga cgggtttacc ggcgtgatgc gcagcccccg
     2041 ctgcacaagc cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg
     2101 cgttcagctt tcaccgcgga ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc
     2161 cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc
     2221 ttttccggtg ttccggcccc cgtcagagtt cgccatagcg agcggctgac cacccgcaaa
     2281 atcaggagca gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg
     2341 tcaggcgtga gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac
     2401 agagccagaa agaggggaaa agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt
     2461 cttttcccct gaagtcagcc acgccccagc ggggatgact tgcatttctc cgcaaggaga
     2521 aaaaagcaac agagataggt taacgtaaaa tgttcagcga aaaaccgaca tggttcacct
     2581 atcctgataa ttgatcgtca gggaatagaa agacttaatc aggggacaat atcccacatc
     2641 agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt
     2701 cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa gttacccaca
     2761 gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg
     2821 cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg
     2881 catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg
     2941 acttaaagat ccccaccgtt tccggtaggt cgctccctct tgcgctctcc tgttccgacc
     3001 ctgccgttta ccggatacct gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca
     3061 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     3121 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactttca tcttgagtcc
     3181 aacccgggaa agacacgcaa aaacgccact ggcagcagcc attggtaact gggagttcgc
     3241 agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac
     3301 actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt
     3361 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg
     3421 gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
     3481 accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc
     3541 cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga
     3601 aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc
     3661 ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg
     3721 tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa
     3781 aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta
     3841 tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa
     3901 tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc
     3961 cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc
     4021 tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct
     4081 tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc gccataccat
     4141 cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct
     4201 ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta
     4261 gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt
     4321 ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag
     4381 gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga
     4441 atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg
     4501 ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc
     4561 tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag
     4621 gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc
     4681 agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga
     4741 cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt
     4801 ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca
     4861 gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga
     4921 acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc
     4981 cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca
     5041 accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc
     5101 gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat
     5161 gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca
     5221 ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca
     5281 tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc
     5341 ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca
     5401 gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta
     5461 ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag
     5521 cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta
     5581 ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct
     5641 cgaccgtctc caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc
     5701 caacatgtcg cggtagggat acccgttacc ggataccccc cgcacagatc ccggcgtgcg
     5761 cgatttacgc accgggctcc tgcctcgggt gtctggcggt gaaccgctcc acaggccatg
     5821 gatgaagaac ccgaaccctt ggtagccatg cggctgccag tttgtttgct ttcgtccagg
     5881 tcgtatcatc cttctggctc ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg
     5941 tcctgaactt ctgcatggtg gggaagttgc ccggtaccga gtgatagttc aggtatccct
     6001 gaaccactct cctgagccat tttccctgtt cggggattga gtaatgccag cgccttcgca
     6061 gaccgtcttt gatggctttc agagttgccg tcatccgatc ccggcgggtc tttcgtatca
     6121 gcatgaacct gccgttgcga tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg
     6181 tttctggttt gccttttccc ctgatggcac ggttttcggc agcgaagcgg ccgaactcca
     6241 tcagacgggt tttctccggg tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg
     6301 ctatacggaa gcgccgggca tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt
     6361 atctgaccat taccacattg cctgtggcat agcgacgtcg ccactgatgc gcccacagat
     6421 cgaagacgta gtggaggtat atgtttgcca gcagcggtga gatgaccgca ccctgtgggg
     6481 tgccttcctc cgttgctcgc cattgaccct cctccgacgt cccggctgtg agccacttac
     6541 gtatgagcct gattaccctc cggtcgccga tccgatgctc tgtgaacctg atcagccatt
     6601 cgtggctcac cctgtcgaag aactgactga tgtcggcatc cagtacccag tttacgttag
     6661 tgcgtaccag ccctgtggcc agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga
     6721 acccgtatga gaaccccata aagtcgtttt catagactgc gttcaggatt ttcaccagcg
     6781 catactggac gatcttgtcc tccagcgagg cgatgccgag cgggcgttgt tttccatccg
     6841 cttttgggat gtagtgacgc ctgccgggct gcgccctgta gctgccctga tgtagcctcc
     6901 ggtgcagatc tgttatgttg ttcttcatgt ttccggcgta gtccatccac ctgatgccat
     6961 ccactccggc ggccgctttc ctgctcaggg agaggaatgc ggcttccagt gcttcgactg
     7021 tcagcaggtg gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca
     7081 cgcgtgacag cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt
     7141 ccgcgttccc cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg
     7201 cctgcttcgc cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg
     7261 actcctcgtc ttcccggtgc gggccatctc cgacactggc agatggtcag aggggagatc
     7321 tcccggttcc cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca
     7381 tgtggcactc gcagtgtcgc accctatgat gttgccttcc gttaacagta caacgtcggc
     7441 acccggtaat ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa
     7501 cgcttcgccc catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg
     7561 tccttcaatg gtcggggact ttcacccctt gatctctaac cggtctcccg gcgcacactg
     7621 tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag
     7681 attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc
     7741 ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac
     7801 atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta
     7861 aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc
     7921 gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa
     7981 gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc
     8041 attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct
     8101 acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg
     8161 tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac
     8221 aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca
     8281 tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg
     8341 tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga
     8401 caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta
     8461 ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag
     8521 tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt
     8581 gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc
     8641 actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca
     8701 gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa
     8761 aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt
     8821 tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc
     8881 cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg
     8941 acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc
     9001 tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa
     9061 attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa
     9121 agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc
     9181 ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc
     9241 agcaaaagcc gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata
     9301 catatgacta caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac
     9361 ttaaggaccg gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc
     9421 tggaggcaat caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg
     9481 gacaggccgc atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg
     9541 aagcctggat aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt
     9601 aacgatttcc gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa
     9661 aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa
     9721 aacattgcca gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt
     9781 gatcgggttt ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca
     9841 gattgtgatc gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct
     9901 ctaataaccg atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca
     9961 gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc
    10021 gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat
    10081 tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc
    10141 atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg
    10201 tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca
    10261 tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa
    10321 aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg
    10381 aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat
    10441 tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac
    10501 ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa
    10561 ttcattaagg atcagtcgct gctgttgctg gaaaagctga acgagctgga catggatacc
    10621 ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg
    10681 gataagctcg gggaagaata acagcaccgg ccatgccggt gctggcgtta ttttttgccg
    10741 aacggccacc accggcgggc gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa
    10801 tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc
    10861 ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct gctccggtga gcacttcccg
    10921 gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccacctttcg atagaggtaa
    10981 tcacacagct tcattcctgt gggtttgtcc cgttcccatt gcggattttc accggtgaaa
    11041 aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc
    11101 atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg
    11161 agcaccacca tgtcttccag atgctcgctg gttggcataa tgcagcgctc gccgtctttg
    11221 tcttccacgt cctcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata
    11281 aatttaaagc acgcgaacag cctctcccgg tccagctcca ccgttttgcg ggcctcgccg
    11341 gtctgtggat tcacgatttc atccaccgcc agaatcgccc gctgtacccg gacaagcgtc
    11401 ggccggtcag ggcagatgta ccagatttca tcgatgccgt tcgcccggcg gttgaacaga
    11461 tacccggaca ccacttcctg ataccgcctg cggctcttga cggtgcgctc tacctcaaat
    11521 gccaccgttc tgccgtccag ctccgccagc gcgtccggca ctttcatccc tttcagggcc
    11581 atacggtgca ggtaccgcca gccggtggcc ccccggccct ccatggagag gcgcagggac
    11641 tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg aaacggaata
    11701 acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc
    11761 cacacgctga cctgacggcc gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc
    11821 accagactgt ccaggaagcg gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca
    11881 accagctccg ccgtggaaca cagctcttcc cgcagccagc gcagggtcag ccgtgttttc
    11941 tccgcgttcc gcgcctgccg cttcgcgtgg ct
//
