LOCUS       NZ_CP143284             4163 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Klebsiella pneumoniae strain B18083 plasmid pB18083_4, complete
            sequence.
ACCESSION   NZ_CP143284
VERSION     NZ_CP143284.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39432603
            Assembly: GCF_036320985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:19:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,608
            CDSs (total)                      :: 5,489
            Genes (coding)                    :: 5,336
            CDSs (with protein)               :: 5,336
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 54 of 153
            Pseudo Genes (incomplete)         :: 104 of 153
            Pseudo Genes (internal stop)      :: 25 of 153
            Pseudo Genes (multiple problems)  :: 26 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143284.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18083"
                     /isolation_source="seawater"
                     /db_xref="taxon:573"
                     /plasmid="pB18083_4"
                     /geo_loc_name="Ireland"
                     /lat_lon="53.27927669 N 9.06174794 W"
                     /collection_date="2018"
                     /collected_by="University of Galway"
     gene            306..497
                     /locus_tag="VST99_RS28020"
                     /old_locus_tag="VST99_28020"
     CDS             306..497
                     /locus_tag="VST99_RS28020"
                     /old_locus_tag="VST99_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032413520.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            497..919
                     /locus_tag="VST99_RS28025"
                     /old_locus_tag="VST99_28025"
     CDS             497..919
                     /locus_tag="VST99_RS28025"
                     /old_locus_tag="VST99_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2038..2229)
                     /locus_tag="VST99_RS28030"
                     /old_locus_tag="VST99_28030"
     CDS             complement(2038..2229)
                     /locus_tag="VST99_RS28030"
                     /old_locus_tag="VST99_28030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            2471..3581
                     /locus_tag="VST99_RS28035"
                     /old_locus_tag="VST99_28035"
                     /pseudo=""
     CDS             2471..3581
                     /locus_tag="VST99_RS28035"
                     /old_locus_tag="VST99_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(3951..4064)
                     /locus_tag="VST99_RS28040"
                     /old_locus_tag="VST99_28040"
     CDS             complement(3951..4064)
                     /locus_tag="VST99_RS28040"
                     /old_locus_tag="VST99_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(3..258)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.393"
                     /coverage="92.0"
                     /evalue="5.27e-68"
     Typing          complement(3952..4163)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.653"
                     /coverage="92.0"
                     /evalue="1.4000000000000001e-98"
ORIGIN
        1 tggcaggaac aggagagcgc acgagggagc catcaggggg aaacgcctgg tatctttata
       61 gtcctgtcgg gtttcgccac cactgatttg agcgtcaaat tctgtgatgc ttgtcagggg
      121 ggcggagcct atggaaaaac ggccgctgtg ctgccttctc cctctccgag ctgtctgctc
      181 tatgtcgtta tgtgcgcgtt gcgaccgctc gccgcagccg aacggcagga gcgtagcgac
      241 tgagtgagcg aggaagcgga aaagagatct gtctgacatt gagcactgac gctctgaggc
      301 cctttatgac gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagcatcagt
      361 ctctgctcct gctggagaag cttaacgagc ttgatctcga tgttgaggcc gacctttgtg
      421 agaaactcca cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgaat
      481 tacaggatgg caattgatgg ccagagtgtc tattagtgaa gcagcacggc ttgttaaggt
      541 tagtcgccct actatttaca agatgattaa cagtgggaag ttaagttaca cctctgttgt
      601 aaagcacggg aaggctatta aagttattga tacttcagag cttattagag tttttggttc
      661 tcttgatggt gtcatcgata ctgtaaaata tgatgtaaaa agtgacgcag aatctacaag
      721 tattaacagc gttggtttac atgatttaca acatagaatc gctctacttg aagctgaaaa
      781 cgatggtctt aaaggggctg ttaaggctcg agatgagcat attgattccc ttcgtcaggc
      841 tatgcagctt ctggagcaca agcgcgagcc ttcttcacca ccccattccc cgtggtggaa
      901 attctggaaa aaatcctgat cctgccatgc ttgagttttc ctctccggcc atcccaggga
      961 gttcctgaac cttcccaaaa atcgccagat ctctgaacgc tcctcacgac taaaaatacc
     1021 cacacactac gcaaaattaa aattttgcag attccttatt taaaacaatt ggttaatgaa
     1081 aaattaatgt gcagtgatgt gttacaaagt gcagatccaa gtcgtggacg actctatggc
     1141 gaatcgttta cgaattgccc acgattcata tgtgaatcac gctttattat catgttgtta
     1201 tgatgaatcg actcttgatt caattcatca atttgggcgt tttttgttca tggtgactct
     1261 tgattcaatt cacgattcga atcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc
     1321 cataacgtga ttgccggccg tcgacgaacc cgattcctga acgtgctgaa aaccggtttt
     1381 ccggctgcgc cggcaacgcc tcacggccac gcaataaatt gcgtccccgt tcaccgtttt
     1441 cggatcagaa gaacgccgga aacaggactt accctggctg aatggcgtga atttccgcgc
     1501 tatgcacttg cgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg
     1561 gttttcgggt ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgaggccgt
     1621 tttagcggtg cgcgtaagaa gacgttatgg taaatccaaa aatgctggca ttcgcaattc
     1681 agtatatcgg tacgcagcat aataatgggg gttaatttcc aacgtatgag ccatcaggtg
     1741 ctaaggttgc ttccgaacca ccaacgtatg agccatcagg tgctaaggtt gcttccgaac
     1801 cactaacgta cgatccatca ggtgctaagg ttacttggga tatcgcggtt agtggtaatg
     1861 ctgacatagc cattaaaacg gcaccaagag ataatgctct catcactatt actcctgtta
     1921 atatcaaaaa tagaattgaa tcgttcagcg gtgccgaacg ccggttccag ccgcgggatt
     1981 cgtgatcggg atgttatagt aaattgatct cttgattttg agtgccctta ctcgaaatca
     2041 ggttgctatt ttcagtgtcg cggctactgc ttgtaccccc ggaagctcct gaagattatt
     2101 attgatagca aactttatct cttcaaagct cgcgttaatc atgagcttct catccacgcc
     2161 acgtaaaaat attattgttt ttgcattacc acatgatttc ataaagataa tatcatcttt
     2221 ttttagcatg atactgtcgt tacttagtgt agtgagataa agcatagcca ctgtttttgt
     2281 gtgtttttgt taaggctgtt aagattattc ctaaccactg attttgtaca tatcatcaaa
     2341 aaaaataaaa aaactaatgc ccccgtggtg ggtatataac aaatcacggg gagcggagct
     2401 gacctgctcc ccgttgatta atacaccgcg atgttagtaa tgtcttcata agccacatga
     2461 ggacatcctc atgaagaagc gtttttccga cgaacagatc atcagtattc tccgcgaggc
     2521 cgaagccggg gtttctgccc gtgagctctg ccgtaagcac gccatttccg acgccatctt
     2581 ttacacctgg cgtaagaagt atggcggtat ggaggtgccc gaggttaagc gccttaagtc
     2641 gcttgaggaa gagaacgcca gactcaagaa actgcttgcc gaagccatgc tggataagca
     2701 ggcgcttcag gtggctctgg ggcgaaagta ctgacgacag accagaagca ggaagccgtt
     2761 gagtttatgt gtgatgcgac cggtctgtcg caacgtagtg cctgcaggct tacaggtttg
     2821 cccctgtcga cctgccgcta tgaggctcag cgttcggcgg ctgatgcgca tttatcactg
     2881 agctggcact ggagcgcagg cgttttggct accgacgcat ctggcagtta ctgcgccgtg
     2941 aaggccttca tgttaatcac aagcgcgtgt accgtcttta ccaccttaac gggctgggtg
     3001 taaaacgcag acgacgtcgt aaaggtctgg caacagaacg tctgccgctg ctccgcccgg
     3061 aggcgcccaa cctgacctgg ccgatggatt ttgtcatgga cgcgctggcc accggtcgca
     3121 ggatcaagtg cctgacctgc gtggacgact tcacgaagga gtgtctgaca attaccgccg
     3181 tcttcgggat ttcaggcgtt caggtcacgc gaattctgga cagcattgca ctgtttcgcg
     3241 gctatccggc gacgataaga actgaccagg ggccggagtt tacctgcaga gcactcgacc
     3301 agtgggctta tgagcatggg gtggagctac ggcttatcca gccgggcagg ccaacacaga
     3361 acggatttat tgaaagtttt aacggacgat tcagggatga gtgcctcaat gagcactggt
     3421 tcagcgatat cgttcacgcc aggaaaacga ttaatgactg gcggcaggat tagagcgagt
     3481 gtcgtccaca ttcatcgctg aactaccaga ctccggctga atttgcggcg ggctggcgaa
     3541 acgggaaata tgaagaaaaa cgaaccgaca ttactaactg aaggttgtat ctaatcctgg
     3601 ggggcaggtc aaccgcgttg gatgattgtg gatcatgatt atatatgttt ttttcttatc
     3661 agaaacaatg ggttaaatag ttttattttg atggttggtg tcgcggagtg gtgctggttt
     3721 cacttcaggg gtaggcatat ggaacaggat cctcttgaga tcctttcttt agcatcgtaa
     3781 tctcttgctc agtaaacgaa aaaaccgccc tgcaaggcgg ttttttcgaa ggttctctga
     3841 gctaccaact ctttgaaccg aggtaactgg cttggaggag cacagtcacc aaaactgttc
     3901 gtttagttta gccttaaccg gcgcataact tcaagtccat ctcctctaaa tcagttacca
     3961 gtggctgctg ccagtggcgc ttttgcatgt cttaccgggt tggactcaag acgatagtta
     4021 ccggataagg cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag
     4081 cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag
     4141 cggaatgaca ccggtaaacc gaa
//
