LOCUS       NZ_CP143094            28545 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Escherichia coli strain EC190486 plasmid pEC190486_2, complete
            sequence.
ACCESSION   NZ_CP143094
VERSION     NZ_CP143094.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39531306
            Assembly: GCF_036287825.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28545)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28545)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 00:41:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,695
            CDSs (total)                      :: 4,574
            Genes (coding)                    :: 4,311
            CDSs (with protein)               :: 4,311
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 73 of 263
            Pseudo Genes (incomplete)         :: 183 of 263
            Pseudo Genes (internal stop)      :: 51 of 263
            Pseudo Genes (multiple problems)  :: 45 of 263
            Pseudo Genes (short protein)      :: 5 of 263
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP143094.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..28545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC190486"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC190486_2"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            complement(66..704)
                     /locus_tag="VZW04_RS23315"
                     /old_locus_tag="VZW04_23315"
     CDS             complement(66..704)
                     /locus_tag="VZW04_RS23315"
                     /old_locus_tag="VZW04_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_024245155.1"
                     /translation="MTQTVPKGFVRAYLRASTSEQDASRALAAIDSFAEERGLNICNYY
                     IENESGSRLDRPELFRLLKDCRHNDILLIEDVDRLSRLTGEDWNSLKKLIRQKDIRVMA
                     INVPTTWIASGSHDFDSRMFAAINDMLLDMLAAVARRDYEQRRERQRQGISKAQKEGRY
                     KGRQANKERYDAINRLLDSGSSWSQVQKIMACSRGTISSAVKSRKLNAI"
                     /name="recombinase family protein"
     gene            1085..1747
                     /locus_tag="VZW04_RS23320"
                     /old_locus_tag="VZW04_23320"
     CDS             1085..1747
                     /locus_tag="VZW04_RS23320"
                     /old_locus_tag="VZW04_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            1845..2126
                     /locus_tag="VZW04_RS23325"
                     /old_locus_tag="VZW04_23325"
     CDS             1845..2126
                     /locus_tag="VZW04_RS23325"
                     /old_locus_tag="VZW04_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            2151..3128
                     /locus_tag="VZW04_RS23330"
                     /old_locus_tag="VZW04_23330"
     CDS             2151..3128
                     /locus_tag="VZW04_RS23330"
                     /old_locus_tag="VZW04_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(3125..3481)
                     /gene="ddp1"
                     /locus_tag="VZW04_RS23335"
                     /old_locus_tag="VZW04_23335"
     CDS             complement(3125..3481)
                     /gene="ddp1"
                     /locus_tag="VZW04_RS23335"
                     /old_locus_tag="VZW04_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     gene            complement(3618..4064)
                     /locus_tag="VZW04_RS23340"
                     /old_locus_tag="VZW04_23340"
                     /pseudo=""
     CDS             complement(3618..4064)
                     /locus_tag="VZW04_RS23340"
                     /old_locus_tag="VZW04_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4104..4940)
                     /locus_tag="VZW04_RS23345"
                     /old_locus_tag="VZW04_23345"
     CDS             complement(4104..4940)
                     /locus_tag="VZW04_RS23345"
                     /old_locus_tag="VZW04_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     gene            6174..6425
                     /locus_tag="VZW04_RS23350"
                     /old_locus_tag="VZW04_23350"
     CDS             6174..6425
                     /locus_tag="VZW04_RS23350"
                     /old_locus_tag="VZW04_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            6415..6696
                     /locus_tag="VZW04_RS23355"
                     /old_locus_tag="VZW04_23355"
     CDS             6415..6696
                     /locus_tag="VZW04_RS23355"
                     /old_locus_tag="VZW04_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6771..7468
                     /locus_tag="VZW04_RS23360"
                     /old_locus_tag="VZW04_23360"
     CDS             join(6771..7020,7020..7468)
                     /locus_tag="VZW04_RS23360"
                     /old_locus_tag="VZW04_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            <7478..7786
                     /locus_tag="VZW04_RS23365"
                     /old_locus_tag="VZW04_23365"
                     /pseudo=""
     CDS             <7478..7786
                     /locus_tag="VZW04_RS23365"
                     /old_locus_tag="VZW04_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            8412..9380
                     /locus_tag="VZW04_RS23370"
                     /old_locus_tag="VZW04_23370"
     CDS             8412..9380
                     /locus_tag="VZW04_RS23370"
                     /old_locus_tag="VZW04_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_030005799.1"
                     /translation="MKDQITHLPDNADHSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSNTHEIICANLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTQLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(9520..9906)
                     /gene="stbA"
                     /locus_tag="VZW04_RS23375"
                     /old_locus_tag="VZW04_23375"
     CDS             complement(9520..9906)
                     /gene="stbA"
                     /locus_tag="VZW04_RS23375"
                     /old_locus_tag="VZW04_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_001039464.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEVQESKKAESP
                     GIIDKEFFKKIGENFDPKKFNKKF"
                     /name="stbA"
     gene            10487..>11389
                     /locus_tag="VZW04_RS23380"
                     /old_locus_tag="VZW04_23380"
                     /pseudo=""
     CDS             10487..>11389
                     /locus_tag="VZW04_RS23380"
                     /old_locus_tag="VZW04_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(11495..>12205)
                     /locus_tag="VZW04_RS23385"
                     /old_locus_tag="VZW04_23385"
                     /pseudo=""
     CDS             complement(11495..>12205)
                     /locus_tag="VZW04_RS23385"
                     /old_locus_tag="VZW04_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /name="APH(6)-I family aminoglycoside O-phosphotransferase"
     gene            complement(12259..12963)
                     /locus_tag="VZW04_RS23390"
                     /old_locus_tag="VZW04_23390"
     CDS             complement(12259..12963)
                     /locus_tag="VZW04_RS23390"
                     /old_locus_tag="VZW04_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <13024..>14646
                     /locus_tag="VZW04_RS23395"
                     /old_locus_tag="VZW04_23395"
                     /pseudo=""
     CDS             <13024..>14646
                     /locus_tag="VZW04_RS23395"
                     /old_locus_tag="VZW04_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(<14722..15737)
                     /locus_tag="VZW04_RS23400"
                     /old_locus_tag="VZW04_23400"
                     /pseudo=""
     CDS             complement(<14722..15737)
                     /locus_tag="VZW04_RS23400"
                     /old_locus_tag="VZW04_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            16132..16788
                     /locus_tag="VZW04_RS23405"
                     /old_locus_tag="VZW04_23405"
     CDS             16132..16788
                     /locus_tag="VZW04_RS23405"
                     /old_locus_tag="VZW04_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(17107..>17535)
                     /locus_tag="VZW04_RS23410"
                     /old_locus_tag="VZW04_23410"
                     /pseudo=""
     CDS             complement(17107..>17535)
                     /locus_tag="VZW04_RS23410"
                     /old_locus_tag="VZW04_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            complement(17568..>18068)
                     /locus_tag="VZW04_RS23415"
                     /old_locus_tag="VZW04_23415"
                     /pseudo=""
     CDS             complement(17568..>18068)
                     /locus_tag="VZW04_RS23415"
                     /old_locus_tag="VZW04_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /name="ISKra4 family transposase"
     gene            complement(18113..18817)
                     /locus_tag="VZW04_RS23420"
                     /old_locus_tag="VZW04_23420"
     CDS             complement(18113..18817)
                     /locus_tag="VZW04_RS23420"
                     /old_locus_tag="VZW04_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(18864..19523)
                     /locus_tag="VZW04_RS23425"
                     /old_locus_tag="VZW04_23425"
     CDS             complement(18864..19523)
                     /locus_tag="VZW04_RS23425"
                     /old_locus_tag="VZW04_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_329491457.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASGHCCK"
                     /name="IS6 family transposase"
     gene            complement(19770..20243)
                     /gene="dfrA14"
                     /locus_tag="VZW04_RS23430"
                     /old_locus_tag="VZW04_23430"
     ARG             complement(19774..20243)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            20399..21511
                     /gene="intI1"
                     /locus_tag="VZW04_RS23435"
                     /old_locus_tag="VZW04_23435"
     CDS             20399..21511
                     /gene="intI1"
                     /locus_tag="VZW04_RS23435"
                     /old_locus_tag="VZW04_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_227088352.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSGHCCK
                     VSDEAAFCLIQRPYISKTLLTRRISPRGSP"
                     /name="intI1"
     gene            complement(21457..22161)
                     /locus_tag="VZW04_RS23440"
                     /old_locus_tag="VZW04_23440"
     CDS             complement(21457..22161)
                     /locus_tag="VZW04_RS23440"
                     /old_locus_tag="VZW04_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <22222..23040
                     /locus_tag="VZW04_RS23445"
                     /old_locus_tag="VZW04_23445"
                     /pseudo=""
     CDS             <22222..23040
                     /locus_tag="VZW04_RS23445"
                     /old_locus_tag="VZW04_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            23082..23324
                     /locus_tag="VZW04_RS23450"
                     /old_locus_tag="VZW04_23450"
     CDS             23082..23324
                     /locus_tag="VZW04_RS23450"
                     /old_locus_tag="VZW04_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032140899.1"
                     /translation="MRKAEREDGRERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(23356..24006)
                     /gene="tetR(A)"
                     /locus_tag="VZW04_RS23455"
                     /old_locus_tag="VZW04_23455"
     CDS             complement(23356..24006)
                     /gene="tetR(A)"
                     /locus_tag="VZW04_RS23455"
                     /old_locus_tag="VZW04_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_001493764.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEADPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            24112..25311
                     /gene="tet(A)"
                     /locus_tag="VZW04_RS23460"
                     /old_locus_tag="VZW04_23460"
     ARG             24113..25308
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(25343..26227)
                     /locus_tag="VZW04_RS23465"
                     /old_locus_tag="VZW04_23465"
     CDS             complement(25343..26227)
                     /locus_tag="VZW04_RS23465"
                     /old_locus_tag="VZW04_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(26365..26757)
                     /locus_tag="VZW04_RS23470"
                     /old_locus_tag="VZW04_23470"
     CDS             complement(26365..26757)
                     /locus_tag="VZW04_RS23470"
                     /old_locus_tag="VZW04_23470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <26761..28512
                     /locus_tag="VZW04_RS23475"
                     /old_locus_tag="VZW04_23475"
                     /pseudo=""
     CDS             <26761..28512
                     /locus_tag="VZW04_RS23475"
                     /old_locus_tag="VZW04_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     ARG             16133..16785
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11496..12211)
                     /name="APH(6)-Id"
                     /gene="APH(6)-Id"
                     /gene_name="APH(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.58"
                     /coverage="85.61"
     Typing          10133..10390
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.273"
                     /coverage="96.0"
                     /evalue="7.34e-49"
     Typing          complement(3619..4064)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattagccac actcgcaagc caagcagagt cggcttaaat tctattgagt gttgtaacta
       61 ctagcttaaa tggcgttcaa cttcctcgat tttacagcac tactaattgt ccctcgacta
      121 caggccataa ttttctgtac ctgactccag gaactaccgc tatcgagcaa ccgattgatg
      181 gcatcgtatc gctccttatt ggcctgtcgc cccttatacc ttccctcttt ttgagcttta
      241 ctaatcccct gtctttgacg ttcccggcgt tgttcatagt cacgacgtgc cacagcagcc
      301 agcatgtcga gcaacatatc gttaattgca gcaaacatgc ggctatcaaa atcatggctt
      361 cctgaagcta tccatgttgt cggaacgttg atggccatca ctcgtatatc tttctgccta
      421 atcaattttt tcaagctgtt ccagtcttcg ccagtcagac gtgagagccg atctacatcc
      481 tcaattaaga gaatatcgtt gtgccggcag tctttgagca acctgaataa ttcaggccgg
      541 tcaagcctcg aaccagattc attctcaatg tagtaattgc aaatgttcag cccacgttcc
      601 tcggcaaaac tatcaatcgc agccagagcg cgagaagcat cttgttcaga tgtagaggcg
      661 cgtaaataag cccggacaaa gcctttcggt acagtttgag tcacagtgta gaaccagttc
      721 gatttaggta gttaaagcat aactatacag tacccgttat gggtggtatt taaaaggcat
      781 actcaaaaag aaccagaaaa cagacctccg accataaagt gacaatgtca cacagaaatt
      841 tacaacataa agatgatgta aagacggtct cagatttacc acgtaaagcc actataaata
      901 cacacaaatc tttacacaat aaagataaca taaatacaag cttatcttta tttgtgcttt
      961 acatggtaaa tcatgtgtaa tataaagaca ctgtaaagac aaaataatct ttacgaacaa
     1021 cacacaatgt aaagataaga cagtctttac ataatcttta cgtcataaag agaggaattc
     1081 tgaaatgggt aaaatcttgc ttgtcgtatc agataagggt ggtgtaggca aaagcaccta
     1141 cgtggctaac acaggctcaa tgctggtcaa taaaggtaag tcggtaatta tcctcaagac
     1201 agataaaaac cacgatctgt tgagctggaa cgaaaagcgg acagataacg gcttactcac
     1261 tatcccggtt cacgcagctt acggaaacgt cagcaatgaa attaagcgac ttagtaagct
     1321 gtgtgaagtc ctcattgtcg attgccccgg ccacgacagc caggagttcc gtagcgccct
     1381 gacagtatca gatatcgtgg taacactggt taagccgtcc tctgatttcg aaagcgaaac
     1441 tctgaccagt gttacagaga aaatccgcac cgctcagaaa gctaacgcag ccctggaacc
     1501 gtgggtgctg ttcaccagaa tcaactcagc gaaaccccgc cacagaaaag cggccattga
     1561 tctggataaa ttactgcgtt cggacaacat ctggattcaa cctcttaaaa cccggatatc
     1621 cgagctggat gtgtatgaaa gcgcctgtaa tgaaggggca ggggtacatg acgttagccg
     1681 ggcatcaagc ctttcaacag cgaaagcaca gattgaacta gtggcgcagg aaattggaat
     1741 tttatagtaa tatcaatacc atctataaat ctttacattg taaagataat ataaagatag
     1801 tatgatattt actattaatg cataccgtac aaggggaaat tgtcatggct ttaaaactaa
     1861 accgtccgaa catcgatgaa tctcagcaac cagcaacaaa cgcagaaacc gcgcgtttta
     1921 tctccggcgc taccagagcg cctgtagtgg gtaaaccaaa gttgatgaac ttcagacttt
     1981 cagcccattt tgaggacata ctggaagctg aagctatcag aacagggcag agcaaaacaa
     2041 ccgttctcaa agcggcgctg gcgatgtaca acagccagga tgaaaacata aagaatcact
     2101 ggttgcttga gtctgcaaaa ttctaaccat agaagcatgg atgaaaatct atgagatatt
     2161 taaccgaatt gatctgtgac ctctacaggt tgacagatca ccataaactg accacagcaa
     2221 cgtttaaaaa tcttgctgat attaagttat ttaaacctac agcagatgca gacaccatat
     2281 tgcagcttga aaatatattt agcgaatact cactgagata tattgacaag gatctggctg
     2341 aaattctgag caacttaata actacagata agaaacactc tctggacttc gattttaaca
     2401 aaatacaatc tctgactgac tcgaaaagtt tcggttgtgg atggagcaag gtaataaatg
     2461 gtagttggtt caaagatctt tattcttggg gagaggggat gtatcctgcc gaaaatctga
     2521 aagctgaaaa tattttagac tggaaagata atatatggca cattgagcat gaaggtttcc
     2581 ccccacgccc ccctattaat gttaaatatt attcttggtt tgaccgatat gtagcttcaa
     2641 acagtggtgg ctcacatcat gcagcaatgg ttgtatatca atcagtcaga gacgggttag
     2701 agtataaaag agaagctgtt attaaacaac tgagtataga cccaggcact gttgaaatac
     2761 ttgataagaa ttactattca ttcatattcc agatcaaaac ccttaataaa gaaactagaa
     2821 tttatacatc tgaatacgag tttacagatg ctttaaaaga ctttggacaa aacagacata
     2881 ccatacttaa acctgttaat tacgtttcca acataaaatt agtatttatt ccaaaagatg
     2941 cattaaaaac aaatagcgag acattccgca actggtttca ttctgcaatt agctgcggaa
     3001 aaataatatc actccctgat tatctgaaaa atccagcaca ttatcataca cttcattact
     3061 cacatgaagt agactcaata acacttggcg acccaagcag aaaatttaaa ctgaaaggag
     3121 attcttagct tttatataaa aaatcaatgt tatcatttgt cagaagatca gcgagggtaa
     3181 tacattcaag aaccacaata tttgttcctt ccaacaaact ggcatcaagt atcttatcga
     3241 agctgataat catcattggc ttgcgttgtg ttaaatcaaa gattaccaat ggaagtatta
     3301 cactgttaat taattcaaac tctttaaaga aagcatcagt gccatactca ctggctggaa
     3361 aaagaatctc aattaatctc acaccataaa aaacatggta gttttcttta aggttatcct
     3421 ttaaaatatt aacagcctta gtatattttt cgttaataaa ctctctcacc tgatattcca
     3481 taaaattctc cgacaagaac cgactgtagg tcaccgggca aacgttgcag aatggcgtca
     3541 gagacgtcat tttgcggcgt ttgccctatc ctgcatcgca gtgaaaaggc agggactggc
     3601 ataactgctg agtagcatca ttgctggctt atctcgctac tgttctcggt gctttcgtcc
     3661 gcaggttcgg gaacaagtaa cttgtgccaa ggcatagttt tgttgagcag ctccatcaga
     3721 ggctcagcca gcagtcccaa aatccaggtt gccgtaatca ccggtatagt cagattttgt
     3781 ggcgtccata actggtgatc gagatgatac caagtgatga cacacaaaat accacccagc
     3841 cagaatacag cccatttaaa cgcaccaaga atggctaaca caaaaacaaa gataaaatgt
     3901 aaaacactcg cgacaagaag acgaagataa aaccatcctc agccaagcca cttaacaaaa
     3961 gtgctccctg ttttcactgc aaaacgtttc ttacgctccc tggcaatttc tttcctgaat
     4021 ttagcacgtt cttccccctg agggaattga tagattttag ccatgagcta cctcatttcg
     4081 atctcaaaac gaagtttatg caattatcct ctgaaaccgt atggtattct gatatctaag
     4141 ccctcacttt ctttatctat tttataagaa aaccttagct ttgatgctct tcttgcgact
     4201 ctctcaacga ttttaacttc gagatgatta atctctgtat attgatttat tttttcaata
     4261 tttttttgca gaaaaacctt attaaaatcc ttaaaattct gataaaggta aactttttcg
     4321 ccattatcta ttctgaataa ttcaagttca tcctttaata catctacttc aatgtcgaaa
     4381 aatgtctttt taccactgct gtattgtttc ctgataaact gataaagcat atttgaattt
     4441 tgatcagcga gtcttacaga cgatatcaaa acctgagtag tgtaagaatc ctttaacatc
     4501 gtgatatatg gctcaatgac atgtgaaaaa gcaatatcaa tatatccttc actttcaaca
     4561 taatcacaaa acgcagtcag gttcataagc ctgactgcat cagaaggtaa tttacctctc
     4621 ttcttcgtat gtccagagct ttgattgccc tgaggtgtga gaagttgatt tcgaggaatg
     4681 cgaataacac tggcctgaag ttcttctgcc ccctctttca actgccggta agcacctgtt
     4741 acatcaatat cagcgataaa tgcatattcc cgagccgtaa tacgaaaggt tgccccctca
     4801 gataactctt cacgtgaatc aatctgcgcc atagccataa acaaaattct tctggcagac
     4861 aaaggcaacg atgagaatgt gctgttaatt tcatttctgt ggcgaatttt tgttttcttt
     4921 gtaacgctca gtaagttcat acattacctg tggtttttaa gttctgatgg tgaaatcata
     4981 accacaaagg cggaaatatg taaagtggtt tatccacctt atgtatttcc accaaaacac
     5041 ggatcacatg aaagaatttt tatcatttat attcaatatg atagaaatgc gcaggtatcc
     5101 accttttaac gtcatacgtt aaattagagc actataaaga actaaagaaa tgaagataca
     5161 aagatacgaa tatctgaata aaaacagaaa aatgcgtacc catccacctt tcagtgcgta
     5221 cccatccacc tttcagtgcg cagccatcca cctttcatga aaattacagt gcgtacccat
     5281 ccacctttca gtgcgtaccc atccaccttt cattttgagg cgacaaaaaa caacacctat
     5341 cttaatgaaa ttattgataa aaaagctggc acaatgtttg cagaataagt taaagtaagt
     5401 taaagatctt aaagtagatc taccgatcta catttaagat cttaaaaaca agaagtttaa
     5461 taagcaaaaa aacacaaaaa atctgtggat aactctgctc aaaacaaaaa attcacagcg
     5521 cctgtggata actttgcgta aacccggagg acagatcact ctgaaccaag acaaccacac
     5581 ataaaaacaa tatgctcatt ttttaaccac tgtgcgtaag tgcctaatgt aatccctgtg
     5641 ctattccgct tcctcgctca ctaactcgct acactcggtc gttcggctgc ggcgagcggt
     5701 gtctcctttc tcaaaaccag aagagttcag ctcatacgat ccgttgattg atcctttttg
     5761 gatctttcac tgagaagccc tgaatcgctt tctgccgctt cgttcgatac aaccatggtg
     5821 aaaccatgcg aaatcgaaaa aatcgctcag aatgcgatac agcgcgtttt agagggtatt
     5881 tacaaaacag tcgttgcctt gaggaaattt tgatcaagat tgaactccta tccggatcat
     5941 ggcaacagat cacagcaaca aggccgctaa aaagcgttga gcggcgttaa aaaccgttaa
     6001 accgtagctg actacaacga aacgggaaaa aacgctcaga acgcgttaca gagcgtttta
     6061 aggcgatgtc ggaaagagcc atatctcacc tgttctgatg tttttctcaa cataagcact
     6121 tttaagagtg cttttaaaga tgctattatg ccaatgagac aagcggaggg cttatgagct
     6181 atcaaattct gacaactaca gcggccagta ttactgacct gaaaaaaaat cctatgggaa
     6241 ccgtagctga aggtgaaggg gacgctgttg cgatcctgaa ccgaaacgaa ccggcgttct
     6301 attgcgttcc accaaaactt tacgcctact atcgggaact cgctgaagat gctgagttaa
     6361 acgctgttgc tgatgagcgc atgaaaaacc cggaaattgt gaaggttaac ctggatgacc
     6421 tatgaactgg cttttgaccg tagagcactg aaggaatggc agaaactcgg ccacaccatc
     6481 cgtgaacaat tcaaaaagaa actggcagaa cggctggaaa atccacgcgt acccgcagcc
     6541 cggttacatg gtcatgctga tcgctataaa atcaaacttc gtgcatctgg ctacagactt
     6601 gtataccaag tcattgatga gaaagtcgtt ttactcgtta ttgccgttgg aagaagggaa
     6661 agcagcgaag tctatcagat cgcagatttg cgctaagatt aatcatcaaa tgattggtga
     6721 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
     6781 tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
     6841 ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca
     6901 cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg
     6961 ttggatgccg ggcaaccgcc cgcattatgg gcgttggcct caacacgatt ttacgtcact
     7021 taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt
     7081 ctgcgcggaa atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct
     7141 gttttacgcg tatgacaggc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac
     7201 tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat
     7261 gacggatggc tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa
     7321 gcgatatacg cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct
     7381 gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca
     7441 ttatctgaac ataaaacact atcagtaagt tggagtcatt accaaccagt tcaccaccta
     7501 ctcgctccgc gattgtggca gtcttcgaaa tccccggacg atccgccttt atgaaagtct
     7561 tgctcaattc aaatcttcag gcttatgggt tactactcat gcttggttaa atgaccgttt
     7621 cgtcttaccg gaatcccagc agaagaactt ggcagagttg aaacgatctt tccttgatcc
     7681 tgcactcaag cagataaatg agaaaacacc tttacttgct aagtatagta ttgatgattc
     7741 aggaaaattt ctgttctcaa taattgataa gcaaaatccc gtctgacata aatcagcaca
     7801 catgagcctg tcatttgaca aatttttgtc atgaagatgg gcggatttcc acacagcacc
     7861 ggcgcccggt aaggtgggcc gattcccata tcgacatgta tgtagcttgt gttatctgtg
     7921 gattgtgcag ctcagcgggt cgctggtcgt atggcggagt gtcccccgta accggccgcg
     7981 tgcggccgct aacgcgcagt acggcgccgc gtcccgcaga cgggccgccg ttcccgcgcg
     8041 cagcactgcg cacccccgtg ggggacgtac ggcagctgcg tggcggtgag cgggattagg
     8101 gctttgcagc taccgtgtta agttccctgg tgtcgctgaa atagttgatg aacatccccc
     8161 taagcgtcag cagcaagctg ccgccgctta ggccccggaa atcacgccct ttacgtcgta
     8221 aacgccgtaa agcccgctca ccggggttaa aagggattac tttgacttct tcattgctgt
     8281 tcgcgagtta aggctaatca gggcaggggg tagggtggca gaatttatag cccggcaacc
     8341 ggctacgccg ggctttcggc gccattttac aacacagacg gctttgttga ataaatcgaa
     8401 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cattccgtgg
     8461 caaagcaaaa gttcaaaata accaactggc ccacctacaa taaagccctc atcaaccgtg
     8521 gctccataac tttctggctg gatgatgaag ctattcaggc ctggtatgag tcggcaacgc
     8581 cttcatcacg gggaagacct cagcgctatt ctgatctcgc catcaccacc gttctggtca
     8641 ttaaacgcgt gttcaggttg accctgcggg ctgcacaggg ttttattgat tccattttta
     8701 cactgatgaa tgttccgttg cgctgcccgg attacaccag tgtcagcaag cgcgcaaagt
     8761 cggttaatgt cagtttcaaa acgttcaccc ggggtgaaat cgcgcatctg gtgattgatt
     8821 ccaccgggct gaaggtcttt ggtgaaggcg aatggaaagt caaaaagcat ggccaggaac
     8881 gccgtcgtat atggcgaaag ttgcatctgg cagttgacag caacacacat gaaatcatct
     8941 gtgcaaacct gtcgctgaac aatgtgacgg actcagaagc cttcccgggt cttatccggc
     9001 agactcacag aaaaatcagg gcagcatcgg cagacggcgc ttacgacacc caactctgtc
     9061 acgatgaact gcggcgtaag aaaatcagcg cgcttatccc gccccgaaaa ggcgcgggtt
     9121 actggcccgg tgaatatgca gaccgtaacc gtgctgttgc gaatcagcgg ctgaccggga
     9181 gtaatgcgcg gtggaaatgg acaacagatt acaaccgtcg ctcgatagcg gaaacggcga
     9241 tgtaccgggt aaaacagctg ttcggaggtt cactgacact gcgtgactac gatggtcagg
     9301 ttgcagaggc tatggccctg gtacgagcgc tgaacaaaat gacgaaagca ggtatgcctg
     9361 aaagcgtgcg tattgcctga aaacacaacc cgctacgggg gagacttacc cgaaatctga
     9421 tttattcaac aaagccgcgc ggggctaaca ggtaagatga aattaaggtt ttacgaacac
     9481 tgccactgta attaattacc gctactttca taaatcacct cagaattttt tgttgaactt
     9541 cttagggtcg aaattctcac cgattttctt gaagaattct ttatcaataa tgccgggact
     9601 ctctgctttt ttactttcct gcacttcagg ctcagacggt tgtgtattgt gcaatacact
     9661 ttccgctgca cccttcgcgc tactcttatc atgcgtattg tgcaatacat tttttcggtt
     9721 ttgccgcgtt ctatacagtg ccatctcaag ccctttggct gttaccgaaa agctgtattc
     9781 gctgtcgagg atttcagcaa tttcatgccg cgttagccct ctgtccagcc agttctcgat
     9841 ttcatttagg cgcaatctga cagcagccga taagttgcgg ggtttcagat ctgctggttt
     9901 attcataacc ccctccgttg tatgaaaatg actctacaac actcgtaaga aggggtcaat
     9961 cgagttttgt agtttatctg tagttttatt gtagtctatt tgtagtctgt cacgtttttt
    10021 gtagtttatt tgtagtttta ttgtagtcta tttgtagtct tacaacacta aggcaaaacg
    10081 ttttttgtag tttatctgta gtttatctgt agtttttggt tgctcagatg gtcatgcaag
    10141 catgaccatc aaagaatagg acgcttttta gccaaatgcc ccttaaccta cggagatcgc
    10201 cctaccaaaa aaagatgatt ttttatccct taacctgcta tacacctaac gcagcgtgat
    10261 aaatcacgct atcgcactga tttaaaagaa aaaaaacaaa atttagccat gctgcggtat
    10321 tacccaggta ttacaaatca aatctaaatt actgatttaa ataacttttt atttattact
    10381 ttggtaatac tatgatttca ccgtattaga cacccttttt tcgtttaaaa aatgcacaaa
    10441 ttgaggactg aatgggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
    10501 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaataaccaa
    10561 ctggcccacc tacaataaag ccctcatcaa ccgtggctcc ataactttct ggctggatga
    10621 tgaagctatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg
    10681 ctattctgac cttgccatca cgactgtgct ggtcattaaa cgcgtattca ggctgaccct
    10741 gcgcgctgcg cagggcttta ttgattccat tttttctctg atgaacgttc cgctacgctg
    10801 cccggattac agctgtgtca gcaggcgggc aaagtcggtt aacgtcagtt tcaaaacgtt
    10861 cacccggggt gaaatcgcgc atctggtgat tgattccacc gggctgaagg tctttggtga
    10921 aggcgagtgg aaagtcaaaa agcatggcca ggaacgccgt cgtatatggc gaaagttgca
    10981 tctggcagtt gacagcaaca cacatgaaat catctgtgca gacctgtcgc tgaacaatgt
    11041 gacggactca gaagccttcc cgggtcttat ccggcagact cacagaaaaa tcagggcagc
    11101 atcggcagac ggcgcttacg acacccggct ctgtcacgat gaactgcggc gtaagaaaat
    11161 cagcgcgctt atccctcccc gaaaaggtgc gggttactgg cccggtgaat atgcagaccg
    11221 taaccgtgca gtggctaatc agcgaatgac cgggagtaat gcgcggtgga aatggacaac
    11281 agattacaac cgtcgctcga tagcggaaac ggcgatgtac cgggtaaaac agctgttcgg
    11341 gggttcactg acgctgcgtg actacgatgg tcaggttgcg gaggctatgg ggtcgtttgc
    11401 gggagagggc gaaatcctac gctaaggctt tggccaacga tattctccgg taagattgat
    11461 gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct
    11521 tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg
    11581 cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg
    11641 cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc
    11701 tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga
    11761 cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc
    11821 catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt
    11881 ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg
    11941 caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca
    12001 gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga
    12061 tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga
    12121 gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat
    12181 cagcaatgtc ttctataggt ttcaatggca ctgttgcaaa gttagcgatg aggcagcctt
    12241 ttgtcttatt caaaggcctt acatttcaaa aactctgctt accaggcgca tttcgcccag
    12301 gggatcacca taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat
    12361 acctttgatg gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgccgattat
    12421 ccgtttcagt ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg
    12481 ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc
    12541 gggcgcttta tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat
    12601 tttacccaga aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac
    12661 agtgcggccc cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac
    12721 gtaggtttca tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag
    12781 ccgtttttcc atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac
    12841 attcactccg cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca
    12901 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
    12961 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
    13021 agtgccgaaa aacatatcac ccgcagggga tacacgctct gctttctcag taaactgcag
    13081 gatagtctga ggcggaggga tgtctacgtt accggcagta accggtgggg agatcctcgt
    13141 gcaagattac tacagggtgc tgactggcag gcaaaccgga ttaaggttta tcgttctttg
    13201 gggcacccga cagacccgca ggaagcaata aaatctctgg gtcatcagct tgatagtcgt
    13261 tacagacagg ttgctgcacg tctttgcgaa aatgaggctg tcgaactcga tgtttctggc
    13321 ccgaagcccc ggttgacaat ttctcccctc gccagtcttg atgagccgga cagtctgaaa
    13381 cgactgagca aaatgatcag tgatctactc cctccggtgg atttaacgga gttgctgctc
    13441 gaaattaacg cccataccgg atttgctgat gagtttttcc atgctagtga agccagtgcc
    13501 agagttgatg atctgcccgt cagcatcagc gccgtgctga tggctgaagc ctgcaatatc
    13561 ggtctggaac cactgatcag atcaaatgtt cctgcactga cccgacaccg gctgaactgg
    13621 acaaaagcga actatctgcg ggctgaaact atcaccagcg ctaatgccag actggttgat
    13681 tttcaggcaa cgctgccact ggcacagata tggggtggag gagaagtggc atctgcagat
    13741 ggaatgcgct ttgttacgcc agtcagaaca atcaatgccg gaccgaaccg caaatacttt
    13801 ggtaataaca gagggatcac ctggtacaac tttgtgtccg atcagtattc cggctttcat
    13861 ggcatcgtta taccggggac gctgagggac tctatctttg tgctggaagg tcttctggaa
    13921 caggagaccg ggctgaatcc aaccgaaatt atgaccgata cagcaggtgc cagcgaactt
    13981 gtctttggcc ttttctggct gctgggatac cagttttctc cacgcctggc tgatgccggt
    14041 gcttcggttt tctggcgaat ggaccatgat gccgactatg gcgtgctgaa tgatattgcc
    14101 agagggcaat cagatccccg aaaaatagtc cttcagtggg acgaaatgat ccggaccgct
    14161 ggctccctga agctgggcaa agtacaggtt tcagtgctgg tccgttcatt gctgaaaagt
    14221 gaacgtcctt ccggactgac tcaggcaatc attgaagtgg ggcgcatcaa caaaacgctg
    14281 tatctgctta attatattga tgatgaagat taccgccggc gcattctgac ccagcttaat
    14341 cggggagaaa gtcgccatgc cgttgccaga gccatctgtc acggtcaaaa aggtgagata
    14401 agaaaacgat ataccgacgg tcaggaagat caactgggca cactggggct ggtcactaac
    14461 gccgtcgtgt tatggaacac tatttatatg caggcagccc tggatcatct ccgggcgcag
    14521 ggtgaaacac tgaatgatga agatatcgca cgcctctccc cgctttgcca cggacatatc
    14581 aatatgctcg gccattattc cttcacgctg gcagaactgg tgaccaaagg acatctgaga
    14641 ccattaaaag aggcgtcaga ggcagaaaac gttgcttaac gtgagttttc gttccactga
    14701 gcgtcagacc cctaaaatcc tgatgctgat gtagtcccgt tttatcgtca tcacgaagct
    14761 ttctgctatg ccattgcttt ccgggcttcg cactgctgtc gtacaaggct ccagtcccag
    14821 caaccgggcg aacgccggca ctgttggcaa ctgccttccg aagcgttttt ccaccgcacc
    14881 cagcatgaca tcctgcaccg tttctttgtc atagcctccg gtgctcgctg cccagtcaat
    14941 gatctccctg tcgcagcagt cctgcgcgaa ggtcacccgc agcttttcgc cattatcaca
    15001 gcggaactca aagccatctg agcaccagcg ccggttactt tccgctactg ctacgcgccc
    15061 cttatgggcc cgcttacggc aaggatcagc gggtttacgt tcaagaagca gattatgcgt
    15121 tctcataatg cgatacaccc gctttgcatt aaccacaggc aggccgtccc gctccgactc
    15181 ccgtcgcagc agcgcccaca cacggcgata accatacgtc ggcaggtcag ccaccgccat
    15241 gtttatccgg gacaatacgg tggtatcgtc agaacggggc tgccgtctgc gatcctgtca
    15301 gtcagatggc cggtgaaccc gaatgctcaa ctgcgcacgc gacacgccaa gacttctgca
    15361 aacgtcgctt attcgtcgtt cccaggcaac gagggcgcat gcgcaatcca tttttttgcc
    15421 cggccgaact ccacggcctc tttgagtatc tcggcttcca tcgtcttttt gcccagaagg
    15481 cgctggagtt cgcgaatttg cttattggcg gcagcgagtt cggaggccgg caccacttcc
    15541 tcgcccgatg caacagccgt cagtgaaccg tcttaatatt gcttgcgcca tttgaatatc
    15601 tggtttgcat tgatgccatg caggcgcgcg acatgagaca cggtcatacc gggctccata
    15661 gtctgctgaa taatggcaat tttttcctgc ggtgtacgac gccgacgccg ctcaggtcct
    15721 gataacactt caaccatctt atcgttctga ctggtattaa acatagttcc aagactacct
    15781 cttattttaa gagagtcgaa gtgtctggtg atctatgggg ccagtctagc cctcttccaa
    15841 gtgtagggag atatagtcaa agtttgcttc tccgccgatg agtccgccct gcgtcggata
    15901 gtccgatttt ttaccggcga agcacggctc tcctaaccct atctaaagcg aataactttt
    15961 ttcaacaggg ccgcgtgcca atgcaagagc gataggcgct ctacgcgcca atttgaccac
    16021 ttaaaacagg taaattgagg ggttgtaatg tgttgatgta acaggctttt attttaatgt
    16081 cttggcatat gtataatggt agtctagccc tcctttcaac aaggagtact catggaaacc
    16141 tacaatcata catatcggca ccacaacttt tcacataaag acttaagtga tctcaccttc
    16201 accgcttgca cattcattcg cagcgacttt cgacgtgcta acttgcgtga tacgacattc
    16261 gtcaactgca agttcattga acagggtgat atcgaaggct gccactttga tgtcgcagat
    16321 cttcgtgatg caagtttcca acaatgccaa cttgcgatgg caaacttcag taatgccaat
    16381 tgctacggta tagagttccg tgcgtgtgat ttaaaaggtg ccaacttttc ccgaacaaac
    16441 tttgcccatc aagtgagtaa tcgtatgtac ttttgctcag catttatttc tggatgtaat
    16501 ctttcctatg ccaatatgga gagggtttgt ttagaaaaat gtgagttgtt tgaaaatcgc
    16561 tggataggaa cgaacctagc gggtgcatca ctgaaagagt cagacttaag tcgaggtgtt
    16621 ttttccgaag atgtctgggg gcaatttagc ctacagggtg ccaatttatg ccacgccgaa
    16681 ctcgacggtt tagatccccg caaagtcgat acatcaggta tcaaaattgc agcctggcag
    16741 caagaactga ttctcgaagc actgggtatt gttgtttatc ctgactaatt gctttgatgt
    16801 gtgattttaa acgctcaaat ttataaaacg aataatattg tcaaccttgc aataatagac
    16861 atagggacaa ccatgtataa atttttaccc tcaggcgtct tcacaataga gccatatagc
    16921 agtgggtcac ttagtgggct taatttttcc attaaagata atattaatat tgctcagtat
    16981 aaaacatcat atggtagccc atcttggcaa gtaaacataa ggctgccatt tataaggcac
    17041 tgttgcaaaa atgtggaggt gataggggta ctacgccgga accccagatt tttccgtccg
    17101 ttcaaattat ccacggttga gcagcttgat cccgtccgcc cgcagttgac caacagggga
    17161 aatatgcctg tacaaagttt gccgggttat cccaagttcc tggcatagcg tactgacctt
    17221 tgtttctgat tgtcccattg atgccattgc cagtcgcagt ttgactggcg tcattttata
    17281 aggtcggccg ccgttccgcc cacgggctct tgctgatgcc agacctgcag tcgttctttc
    17341 agcaatcagt tcgcgctcaa actccgccag tgcggcaaag ataccgaaga caagcttgcc
    17401 agcggccgtt gttgtgtcga tagtcgcccc gtgaccggtc agcactttca gaccggtccc
    17461 cctcgcggtc aggtcgtgca ctgtattaat gagatgacga agatcacgac cgaggcggtc
    17521 cagcttccat acgaccgaga gcataaaaaa tattggggca gcgtgagcta tgctgccatc
    17581 gctggtccct tcccgtcact ttgaaaaccc ggataccagc agacaaattt atcctgcagt
    17641 tcgttattca aaacggcggt ccgggtctgt aacaaataat gagccccttt tctgctccac
    17701 tgcatctgct gctttttggc cattcgtctg gcgatcactt cattgatcgt ggattccaca
    17761 aacgcggttg ataccggctc tccgtaccga cgcatttcgc cgtagtttgg gatcatcatc
    17821 ttattattcc ggatgtaggt atacatttca tccagatgtt tttgcaggga tttcaggcta
    17881 ggatagctga gttcagggtc atcgcaatac ataacgcaat tatcaatatg ttccagcgca
    17941 gcgacaacat tgccatgcca gagatatcgt ttaatgcttt ccagcagtgc cagaacttta
    18001 ctccccgcct ctggatctga taccagcagt ccccgggcat attgcatgag caccttcagc
    18061 ggcactgttg caaagttagc gatgaggcag ccttttgtct tattcaaagg ccttacattt
    18121 caaaaactct gcttaccagg cgcatttcgc ccaggggatc accataataa aatgctgagg
    18181 cctggccttt gcgtagtgca cgcatcacct caataccttt gatggtggcg taagccgtct
    18241 tcatggattt aaatcccagc gtggcgccga ttatccgttt cagtttgcca tgatcgcatt
    18301 caatcacgtt gttccggtac ttaatctgtc ggtgttcaac gtcagacggg caccggcctt
    18361 cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc
    18421 gcgggatctg ccacttcttc acgttgttga ggattttacc cagaaaccgg tatgcagctt
    18481 tgctgttacg acgggaggag agataaaaat cgacagtgcg gccccggctg tcgacggccc
    18541 ggtacagata cgcccagcgg ccattgacct tcacgtaggt ttcatccatg tgccacgggc
    18601 aaagatcgga agggttacgc cagtaccagc gcagccgttt ttccatttca ggcgcataac
    18661 gctgaaccca gcggtaaatc gtggagtgat cgacattcac tccgcgttca gccagcatct
    18721 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
    18781 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
    18841 gatgataagt ttatcaccac cgactatttg caacagtgcc ctgaggcctg gcctttgcgt
    18901 agtgcacgca tcacctcaat acctttgatg gtggcgtaag ccgtcttcat ggatttaaat
    18961 cccagcgtgg cgccgattat ccgtttcagt ttgccatgat cgcattcaat cacgttgttc
    19021 cggtacttaa tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg tttgagcaga
    19081 gcaagcgcgc gaccataggc gggcgcttta tccgtgttga tgaatcgcgg gatctgccac
    19141 ttcttcacgt tgttgaggat tttacccaga aaccggtatg cagctttgct gttacgacgg
    19201 gaggagagat aaaaatcgac agtgcggccc cggctgtcga cggcccggta cagatacgcc
    19261 cagcggccat tgaccttcac gtaggtttca tccatgtgcc acgggcaaag atcggaaggg
    19321 ttacgccagt accagcgcag ccgtttttcc atttcaggcg cataacgctg aacccagcgg
    19381 taaatcgtgg agtgatcgac attcactccg cgttcagcca gcatctcctg cagctcacgg
    19441 taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa
    19501 tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat
    19561 caccaccgac tatttgcaac agtgccgtcc tgcgaatatg tgagttttga cctaatgagc
    19621 cacaaaaact cattttcgcc cacgataatt tttctacttc ctttctttgg aatgctcatt
    19681 tcaatattca cataacgtcg ctatcaatcg cggaaaaggc gtagtgcgcg aagcgaacga
    19741 agctttttgc cgtcgattgc atagctttgt taaccctttt tccaaatttg atagcaatag
    19801 ttaatgtttg aagtaaagtg ttgctcaaaa acaacttcga aggtatttgg aatactcggg
    19861 aagaaaacat ccccctctgg ctcgatgtcg atcgtcgata agtggagcgt agaggccatg
    19921 ggtaatgttt ctcggtaaat ttctccgcca ccagacacta taacgtgacc ggtgaattca
    19981 gctagcctgt ccatggcctc ttcgattgac tgaaatacaa ctacattgtc atcatttgat
    20041 gtccaacctg agcgggtaac gaccgcgtat ttcctattgg ggagtgcgcc catagattca
    20101 aacgtcttgc gacccaccag aagccactga ttgtaggtca atgctttaaa aagtagctgc
    20161 tcccctttcg cggaccaggg tatgtctgga ccgcaaccaa tcacgccgtt tttcgctttc
    20221 gcagccatca atgatacttt caaggttctc atcctgggtt aactttgttt tagggcgact
    20281 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
    20341 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
    20401 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
    20461 tgagcgcata cgctacttgc attacagctt accaaccgaa caggcttatg tccactgggt
    20521 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
    20581 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
    20641 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
    20701 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
    20761 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc agcttctgta
    20821 tggaacgggc atgcggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga
    20881 tcacggcacg atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgttacc
    20941 cgagagcttg gcacccagcc tgcgcgagca gctgtcgcgt gcacgggcat ggtggctgaa
    21001 ggaccaggcc gagggccgca gcggcgttgc gcttcccgac gcccttgagc ggaagtatcc
    21061 gcgcgccggg cattcctggc cgtggttctg ggtttttgcg cagcacacgc attcgaccga
    21121 tccacggagc ggtgtcgtgc gtcgccatca catgtatgac cagacctttc agcgcgcctt
    21181 caaacgtgcc gtagaacaag caggcatcac gaagcccgcc acaccgcaca ccctccgcca
    21241 ctcgttcgcg acggccttgc tccgcagcgg ttacgacatt cgaaccgtgc aggatctgct
    21301 cggccattcc gacgtctcta cgacgatgat ttacacgcat gtgctgaaag ttggcggtgc
    21361 cggagtgcgc tcaccgcttg atgcgctgcc gcccctcact agtgggcact gttgcaaagt
    21421 tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac
    21481 caggcgcatt tcgcccaggg gatcaccata ataaaatgct gaggcctggc ctttgcgtag
    21541 tgcacgcatc acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc
    21601 cagcgtggcg ccgattatcc gtttcagttt gccatgatcg cattcaatca cgttgttccg
    21661 gtacttaatc tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc
    21721 aagcgcgcga ccataggcgg gcgctttatc cgtgttgatg aatcgcggga tctgccactt
    21781 cttcacgttg ttgaggattt tacccagaaa ccggtatgca gctttgctgt tacgacggga
    21841 ggagagataa aaatcgacag tgcggccccg gctgtcgacg gcccggtaca gatacgccca
    21901 gcggccattg accttcacgt aggtttcatc catgtgccac gggcaaagat cggaagggtt
    21961 acgccagtac cagcgcagcc gtttttccat ttcaggcgca taacgctgaa cccagcggta
    22021 aatcgtggag tgatcgacat tcactccgcg ttcagccagc atctcctgca gctcacggta
    22081 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
    22141 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
    22201 ccaccgacta tttgcaacag tgccccgttc agcacccgcg tggtgaatgt cggcgtccgc
    22261 gattccacct atgtgctcga cggcctgctg taccacgagt ccgacctgcg gatcgaggag
    22321 cactacaccg acacggccgg cttcaccgat cacgtctttg ccctgatgca cctgctaggc
    22381 ttccgcttcg cgccgcgcat ccgcgacctc ggcgaaacca agctgtacgt gccgcagggc
    22441 gtgcaagcct acccgacgtt gcgcccgctg atcggcggca ccctgaacat caagcacgtg
    22501 cgtgcccact gggacgacat cctgcgcctg gccagctcga tcaagcaggg caccgtcacc
    22561 gcctcgctga tgctgcgcaa gctcggcagc tacccgcgcc agaacggact ggccgtggcc
    22621 ctgcgcgagc tgggccggat cgagcgcacg ctgttcatcc tggactggct gcaaagtgtt
    22681 gaactgcgcc gccgcgtgca tgccggcctg aacaaaggtg aggcgcgcaa ctcgctggcc
    22741 agggcggtgt tcttcaaccg ccttggggaa atcagggatc ggagcttcga gcagcagcgc
    22801 taccgggcca gcggcctcaa cctggtgacg gcggctatcg tgctgtggaa cacggtgtac
    22861 ctggaacgcg ccacccaggg gttggtcgag gccggcaagc cggtggacgg cgagctgctg
    22921 caattcctgt cgccgctggg ctgggagcac atcaacctaa ccggcgatta cgtctggcgg
    22981 cagagccgca gactggaaga cgggaagttt cggcccttac ggatgcccgg aaaaccttag
    23041 cgtacgattt tttccgaatt ctgcgggctc ccctatctca tctgcgcaag gcagaacgtg
    23101 aagacggccg cgagcgccag gcgcacgagg ccggcgcgcg gacccgcgcc acggcccacg
    23161 agcggacgcc gcagcaggag cgccagaagg ccgccagaga ggccgagcgc ggccgtgagg
    23221 cttggacgct agggcagggc atgaaaaagc ccgtagcggg ctgctacggg cgtctgacgc
    23281 ggtggaaagg gggaggggat gttgtctaca tggctctgct gtagtgagtg ggttgcgctc
    23341 cggcagcggt cctgatcaat cgtcaccctt tctcggtcct tcaacgttcc tgacaacgag
    23401 cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg ctgcgtccgg
    23461 atcggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg gagcctgttc
    23521 aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa gcacggcccc
    23581 aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg aaaaacccgc
    23641 ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc ccggtcgcgt
    23701 gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga agctgcgggc
    23761 attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat gcgtatgatt
    23821 ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca
    23881 gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg tcagaccgtc
    23941 tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg gctgcaactt
    24001 tgtcatgctt gacactttat cactgataaa cataatatgt ccaccaactt atcagtgata
    24061 aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga cgtgaaaccc
    24121 aacagacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg catcggcctg
    24181 attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga cgtcaccgcc
    24241 cactatggca ttctgctggc gctgtatgcg ttgatgcaat ttgcctgcgc acctgtgctg
    24301 ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc tcgtctcgct ggccggcgct
    24361 gctgtcgact acgccatcat ggcgacggcg cctttccttt gggttctcta tatcgggcgg
    24421 atcgtggccg gcatcaccgg ggcgactggg gcggtagccg gcgcttatat tgccgatatc
    24481 actgatggcg atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt cgggttcggg
    24541 atggtcgcgg gacctgtgct cggtgggctg atgggcggtt tctcccccca cgctccgttc
    24601 ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg gctgtttcct tttgccggag
    24661 tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc tcaacccgct cgcttcgttc
    24721 cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg cggtcttctt catcatgcaa
    24781 cttgtcggac aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg ctttcactgg
    24841 gacgcgacca cgatcggcat ttcgcttgcc gcatttggca ttctgcattc actcgcccag
    24901 gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact catgctcgga
    24961 atgattgccg acggcacagg ctacatcctg cttgccttcg cgacacgggg atggatggcg
    25021 ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct gcaagcaatg
    25081 ttgtccaggc aggtggatga ggaacgtcag gggcagctgc aaggctcact ggcggcgctc
    25141 accagcctga cctcgatcgt cggacccctc ctcttcacgg cgatctatgc ggcttctata
    25201 acaacgtgga acgggtgggc atggattgca ggcgctgccc tctacttgct ctgcctgccg
    25261 gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag ccgatcgctg atcgtggaaa
    25321 cgataggcct atgccatgcg ggtcaaggcg acttccggca agctatacgc gccctaggag
    25381 tgcggttgga acgttggccc agccagatac tcccgatcac gagcaggacg ccgatgattt
    25441 gaagcgcact cagcgtctga tccaagaaca accatcctag caacacggcg gtccccgggc
    25501 tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa
    25561 ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc
    25621 ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc
    25681 cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg
    25741 tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag
    25801 gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag
    25861 cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga
    25921 ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg
    25981 aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca
    26041 tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca
    26101 acgcccgcag catcgcgacc gtcatcggtg agaagttcgg caggtattgg gtggtgacaa
    26161 tgtaggtgct gccccaaatg gcaggtgcta tcgctgtgaa caataaatcg ggcgtgcgta
    26221 atgacatgcg attacaagac ctccgctacg gcgatgggtt tttgagagtt gcaggcgccg
    26281 cgatgaccgc accctgactt ggactggccc tgcctcgcac caatccacct gcggctatgg
    26341 ccgcgatcat gcgaggcccg aaaattacac gacctgtaca cgtccgccga acccggaacg
    26401 caacagagct tcgtgggcat cccgatccgg gtcgtagcag cagtcctgga ccaaacgcac
    26461 gtcgtagtcc gcatcactag cccaggcgac gcttgaaaga acaacgccgg tggtgcttat
    26521 cccggccatg acaagcgtgc ttacgccgcg cgtccgaagg tcggcgtcga gcgccgtgcc
    26581 ataaaagacg ctggctcgcg gacatgcata gaaaaggtcg ccccgttcga tcgcaagccc
    26641 ttcgacgggc agaccggtgc gaaatcgtcc gctcgggaga tacggtgaga tttggcgatt
    26701 cgtcgccggc ggcgcatgtt catactcttc gccgagcgaa aagttgggga acagcacggg
    26761 ccggccgcgc aaggcgtgct ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac
    26821 ctgcgcaagg tgccggccga tgcacccacg gccttcatca agccgcgctg gaagccgctg
    26881 gtgatcaccc cggaaggcct cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg
    26941 aagaacgccc tgcgctccgg cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc
    27001 gacgactacc tgctgccggc cgagaagttc gccgcactca agcgcgagca ggccctgccc
    27061 ctggcgatca acccgaacag cgaccagtac ctggaagagc gtttgcagct gctggacgag
    27121 cagttggcca ccgtcacccg cctggccaag gacaacgagc tgcccgatgc catcctcacc
    27181 gagtcagggc tgaaaatcac cccgctggat gcggcggtgc cggatcgggc gcaggcgctg
    27241 atcgaccaaa ccagccagtt actgccgcgc atcaagatca ccgaactgct gatggacgtg
    27301 gacgactgga cgggcttcag ccgccacttc acccacttga aggacggggc cgaggccaaa
    27361 gacaggacgt tgctgctgtc cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag
    27421 atggccgagt cgagccccgg cctgacctac gccaagctgt cctggctgca agcctggcac
    27481 atccgcgacg aaacctattc ggcggccttg gccgagctgg tcaaccacca gtatcgccac
    27541 gcctttgccg cccactgggg cgacggcacg acctcatcct ccgatggcca gcgcttccgc
    27601 gcgggtggcc ggggcgagag caccgggcac gtcaacccga agtacggtag cgagccggga
    27661 cggctgttct atacccatat ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat
    27721 gtcggcgtcc gcgattccac ctatgtgctc gacggcctgc tgtaccacga gtccgacctg
    27781 cggatcgagg agcactacac cgacacggcc ggcttcaccg atcacgtctt tgccctgatg
    27841 cacctgctag gcttccgctt cgcgccgcgc atccgcgacc tcggcgaaac caagctgtac
    27901 gtgccgcagg gcgtgcaagc ctacccgacg ttgcgcccgc tgatcggcgg caccctgaac
    27961 atcaagcacg tgcgtgccca ctgggacgac atcctgcgcc tggccagctc gatcaagcag
    28021 ggcaccgtca ccgcctcgct gatgctgcgc aagctcggca gctacccgcg ccagaacgga
    28081 ctggccgtgg ccctgcgcga gctgggccgg atcgagcgca cgctgttcat cctggactgg
    28141 ctgcaaagtg ttgaactgcg ccgccgcgtg catgccggcc tgaacaaagg tgaggcgcgc
    28201 aactcgctgg ccagggcggt gttcttcaac cgccttgggg aaatcaggga tcggagcttc
    28261 gagcagcagc gctaccgggc cagcggcctc aacctggtga cggcggctat cgtgctgtgg
    28321 aacacggtgt acctggaacg cgccacccag gggttggtcg aggccggcaa gccggtggac
    28381 ggcgagctgc tgcaattcct gtcgccgctg ggctgggagc acatcaacct aaccggcgat
    28441 tacgtctggc ggcagagccg cagactggaa gacgggaagt ttcggccctt acggatgccc
    28501 ggaaaacctt agcgtacgat tttttccgaa ttctgcgggc tcccc
//
