LOCUS       NZ_CP142986            15032 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CRE-22 plasmid pKP22-2, complete
            sequence.
ACCESSION   NZ_CP142986
VERSION     NZ_CP142986.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39484187
            Assembly: GCF_036287375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 15032)
  AUTHORS   Tran,T.T.D.
  TITLE     Hypervirulent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15032)
  AUTHORS   Tran,T.T.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2024) Institute of Microbiology and Immunology,
            National Yang Ming Chiao Tung University, Linong St, Taipei 112,
            Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. Sep 2023
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 12:39:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,014
            CDSs (total)                      :: 5,891
            Genes (coding)                    :: 5,706
            CDSs (with protein)               :: 5,706
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 102 of 185
            Pseudo Genes (incomplete)         :: 90 of 185
            Pseudo Genes (internal stop)      :: 23 of 185
            Pseudo Genes (multiple problems)  :: 30 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142986.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15032
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRE-22"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP22-2"
                     /geo_loc_name="Taiwan"
                     /lat_lon="24.93920382 N 121.36300653 E"
                     /collection_date="2017"
                     /collected_by="Ya-Min Tsai"
     gene            486..2447
                     /locus_tag="VWZ89_RS27245"
                     /old_locus_tag="VWZ89_27250"
     CDS             486..2447
                     /locus_tag="VWZ89_RS27245"
                     /old_locus_tag="VWZ89_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_065799589.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2447..3037
                     /locus_tag="VWZ89_RS27250"
                     /old_locus_tag="VWZ89_27255"
     CDS             2447..3037
                     /locus_tag="VWZ89_RS27250"
                     /old_locus_tag="VWZ89_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_231828915.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYRGLTLSGTKTHVKGFWS"
                     /name="replication-relaxation family protein"
     gene            complement(3132..3992)
                     /locus_tag="VWZ89_RS27255"
                     /old_locus_tag="VWZ89_27260"
     CDS             complement(3132..3992)
                     /locus_tag="VWZ89_RS27255"
                     /old_locus_tag="VWZ89_27260"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(4175..4732)
                     /locus_tag="VWZ89_RS27260"
                     /old_locus_tag="VWZ89_27265"
     CDS             complement(4175..4732)
                     /locus_tag="VWZ89_RS27260"
                     /old_locus_tag="VWZ89_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            4896..7901
                     /locus_tag="VWZ89_RS27265"
                     /old_locus_tag="VWZ89_27270"
     CDS             4896..7901
                     /locus_tag="VWZ89_RS27265"
                     /old_locus_tag="VWZ89_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <7936..8133
                     /locus_tag="VWZ89_RS27270"
                     /old_locus_tag="VWZ89_27275"
                     /pseudo=""
     CDS             <7936..8133
                     /locus_tag="VWZ89_RS27270"
                     /old_locus_tag="VWZ89_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            8140..8670
                     /locus_tag="VWZ89_RS27275"
                     /old_locus_tag="VWZ89_27280"
     CDS             8140..8670
                     /locus_tag="VWZ89_RS27275"
                     /old_locus_tag="VWZ89_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8698..8877)
                     /locus_tag="VWZ89_RS27280"
                     /old_locus_tag="VWZ89_27285"
     CDS             complement(8698..8877)
                     /locus_tag="VWZ89_RS27280"
                     /old_locus_tag="VWZ89_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8903..9331)
                     /locus_tag="VWZ89_RS27285"
                     /old_locus_tag="VWZ89_27290"
     CDS             complement(8903..9331)
                     /locus_tag="VWZ89_RS27285"
                     /old_locus_tag="VWZ89_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(9495..9809)
                     /locus_tag="VWZ89_RS27290"
                     /old_locus_tag="VWZ89_27295"
     CDS             complement(9495..9809)
                     /locus_tag="VWZ89_RS27290"
                     /old_locus_tag="VWZ89_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9797..10075)
                     /locus_tag="VWZ89_RS27295"
                     /old_locus_tag="VWZ89_27300"
     CDS             complement(9797..10075)
                     /locus_tag="VWZ89_RS27295"
                     /old_locus_tag="VWZ89_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(10417..10635)
                     /locus_tag="VWZ89_RS27300"
                     /old_locus_tag="VWZ89_27305"
     CDS             complement(10417..10635)
                     /locus_tag="VWZ89_RS27300"
                     /old_locus_tag="VWZ89_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(10632..11003)
                     /gene="tolA"
                     /locus_tag="VWZ89_RS27305"
                     /old_locus_tag="VWZ89_27310"
     CDS             complement(10632..11003)
                     /gene="tolA"
                     /locus_tag="VWZ89_RS27305"
                     /old_locus_tag="VWZ89_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(11277..11522)
                     /locus_tag="VWZ89_RS27310"
                     /old_locus_tag="VWZ89_27315"
     CDS             complement(11277..11522)
                     /locus_tag="VWZ89_RS27310"
                     /old_locus_tag="VWZ89_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            11849..13534
                     /locus_tag="VWZ89_RS27315"
                     /old_locus_tag="VWZ89_27320"
     CDS             11849..13534
                     /locus_tag="VWZ89_RS27315"
                     /old_locus_tag="VWZ89_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            13544..13801
                     /locus_tag="VWZ89_RS27320"
                     /old_locus_tag="VWZ89_27325"
     CDS             13544..13801
                     /locus_tag="VWZ89_RS27320"
                     /old_locus_tag="VWZ89_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            13886..14035
                     /locus_tag="VWZ89_RS27325"
                     /old_locus_tag="VWZ89_27330"
     CDS             13886..14035
                     /locus_tag="VWZ89_RS27325"
                     /old_locus_tag="VWZ89_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     ARG             complement(3136..3992)
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     BGC             6849..15032
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(14139..14717)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2448..2983
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.855"
                     /coverage="100.0"
                     /evalue="7.89e-97"
     Typing          7913..8130
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.671"
                     /coverage="100.0"
                     /evalue="1.86e-33"
ORIGIN
        1 cttcaggggg aaatcccctg cttacgctgg ctggacgcca ccgacgcact gtctttcccc
       61 ctctttctgg ctctgtccgg ctggacgccg gtcacgccag aaattcaccc ggcccccgtc
      121 aggctcacgc ctgaccaccc acaaaatcca cggtgctgga cgcacctgac gaagcagcaa
      181 agctgctcct gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg
      241 gaacaccgga aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct
      301 cgttgttcct ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg
      361 tgaaagctga acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc
      421 gcggcttgtg cagcaggggc tgcacaacac gccggtaaac ccgtcgtctg aaggagagag
      481 attgcatgtt taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc
      541 agatttaccc gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc
      601 tggttatcgg cctgtttttc ggtctggcag aactctgggc cgggcggatg cgattcgtgg
      661 ggctgagtca taaggagcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga
      721 ctgcgccatt ttcggtgctg gcctcgctgg attcgcccgg gcggatggtc agggcacggc
      781 gacatgtctg gaactacgca tactggctga ccggcgctgc cggtctggcc cttctggggg
      841 ggctggttgt cgccgtgttt atcgagagct ggggagaaac cgtcctgcgt ccggacctct
      901 acgcgaacgc agattattac ctgctgcagc cggctaacct gttcgggatt gcctggtgtg
      961 ccggaggtct tgtcgcaggc gtcctcctgg cgcggctgct ggcgttcctg ctgcttgacg
     1021 ggcattttgc ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca
     1081 gcagccagcg gaccggagaa atgaccgacg tccgccacct gcatttcggg gagccggtac
     1141 cggttaacgc gctggccgat ttttccacgg agcaggcccg gaaacagcag gcggtgtttc
     1201 tgggtaagga tgagcagggc cagccggtgc tggtcccgcg tgatacctgg cgcaagacca
     1261 acattcagat cctcggcctg cccgggagcg gcaagagcgt gatggccacc aacgcgctca
     1321 ttcgctgcgt acgcgatttc ggggacgcgg tggtgtactt cgacccgaaa ggggacgcgt
     1381 gggcgccgca cgtcttccgg gcgcactgcc cggacttcac cctgctggat ctgcggccgg
     1441 ggaaaccggc gcagctgaat ctgttccgcg atctcgacca gtacgccctg aagaatcttc
     1501 tggttgccgg atttaacctg agtgagaccg gcgacgtggc ggaccactac cgcatcagcg
     1561 agcagaaagc ggcgaagctg attgcggagc agtttccgca gggggcaaat attcagcagg
     1621 tgctggcggc ggcgtacgca ctgccggagg ccctgaaaaa ggacgtgaag gggctgatca
     1681 ccaagctgga gaacgtggcc gacctgagcg tccttcagac ggacagcggc atcgacgtgg
     1741 cggggataat caacggcggc ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg
     1801 tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg
     1861 agttccggtg gtggccgcac gcgagcatca tgctcgatga gattaaatat ctgctgtcga
     1921 agtacgtgct gaacgcgctg gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc
     1981 accagtcgct gggcgacttc ggccagtgcg ggcaggatct gcccgctgat tttgtgaaaa
     2041 ccacggtact ggataacacg ccgatccgct ggttctaccg ggcggccagc caggagtccg
     2101 ctcagtgggc ggccggacag accggggaga tccgggtgga cgtggagcgc cgccgggcca
     2161 gccgggaggc ggggaacgtg gagcatatca gcggggacag cttcatccag aaggaggcgc
     2221 ggccgctgtt tgacgtgaac accctgcaac atctgcctga cgggttcgcg gtgatgaccg
     2281 ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg tgttgatcgc cgggaaattc
     2341 cgctgaaaac cttccctgtg ctggcgaaaa cggacccgct ggcggagtac cagtcggagg
     2401 ccgggcggca gcggccgggt gatgatgatt ttgcggggct gtactgatgg cactggagcg
     2461 atacaacgtc agccacgcga agcggcaggc acggaacgcg gagaaaacac ggctgaccct
     2521 gcgctggctg cgggaagaac tgtgttccac ggcggagctg gttgcgcaac ggctggggat
     2581 tgcggcggta cagccggttt accgcttcct ggacagcctg gtggcgaaag ggctgctggt
     2641 ccgggcgaaa tacccggttg acggccgtca ggtcagcgtg tgggggctga cgccgcacgg
     2701 ggtggcgttc tcgttcgacg aggacgagcc gctgacggac attattccgt ttcagccgtc
     2761 gcgggtgtcg gcggcgcagc tgccgcaccg gctggccgtg cagtccctgc gcctcgccat
     2821 ggaggcccgg ggggccaccg gctggcggta cctgcaccgt attgccctga aagggatgaa
     2881 ggtgccggac gcgctggcgg agctggacgg cagaacggtg gcgtttgagg tggaacgtac
     2941 cgtcaagagc cgtcgccggt atcaggaagt ggtgtccggg tatcggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3421 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
     3481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggcagca
     3601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3721 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcaccaca tagcagaact
     3781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3961 ataagggcga cacgaaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4021 atttaccagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4141 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     4201 ttttataaac cgtggagcgg gcaatactga gctgatgagc aatttccgtt gcaccagtgc
     4261 ccttctgatg aagcgtcagc acgacgttcc tgtccacggt acgcctgcgg ccaaatttga
     4321 ttcctttcag ctttgcttcc tgtcggccct cattcgtgcg ttctaggatc ctccggcgtt
     4381 cagcctgtgc cacagccgac aggatggtga ccaccatttg ccccatatca ccgtcggtac
     4441 tgatcccgtc atcaatgaac cggactgcca cgccctgagc gtcaaattcc tttatcagtt
     4501 ggatcatatc ggcagtgtcg cggccaagac ggtcgagctt cttaaccaga atgacatcac
     4561 cttcctccac cttcatcctc agcaaatcca gcccttcccg gtctgttgaa ctgccggatg
     4621 ccttatcggt aaatatacgg tttgctttca cacctgcgtc tttgagtgct ctgacctgaa
     4681 gatcaagaga ctgctgactg gttgagaccc gagcgtaacc aaaaagtcgc ataaaaatgt
     4741 accttaaatc gaatatcgga caactcatgt ctattattac aaatttacga tttaatagac
     4801 atattaatgt aacagtttta cgatgtccga taatttataa catttcgtac ggttggaaaa
     4861 atgttactaa atgcccgtca ggcagggagg ccgatatgcc cgttgacttt ctgaccactg
     4921 agcagactga aagctatggc agattcaccg gtgaaccgga tgagcttcag ctggcacgat
     4981 attttcacct tgatgaagca gacaaggaat ttatcggaaa aagcagaggt gatcacaacc
     5041 gtctgggcat tgccctgcaa attggatgtg tccgttttct gggcaccttc ctcaccgata
     5101 tgaatcatat tccttccggc gtccggcatt ttaccgccag acagctcggg attcgtgata
     5161 tcaccgttct tgcagaatac ggtcagaggg aaaatacccg ccgtgagcat gcagcgctga
     5221 tacgtcagca ctatcagtat cgtgaatttg cctggccctg gacatttcgc cttacccgtc
     5281 ttttatatac ccggagctgg ataagcaacg aacgtcctgg cctgcttttc gatctggcga
     5341 cagggtggct tatgcaacat cgtattattc tccccggagc cactacgctg acccggttga
     5401 tttcagaggt aagggaaaag gcgacgttgc gcctgtggaa caaactggca ctgataccgt
     5461 cagccgaaca gcgttcacag ctggagatgc tgctggggcc aactgattgc agccgcctgt
     5521 ctttactgga atcactgaaa aagggccctg tgaccatcag tggtccggcg tttaatgaag
     5581 caattgaacg ctggaaaact ctgaacgatt ttggcctgca tgctgaaaac ctgagtacac
     5641 tcccggctgt gcgcctgaaa aatctcgcac gttatgctgg tatgacttcg gtgttcaata
     5701 ttgccaggat gtcaccgcag aaaaggatgg cggttctggt tgcctttgtc cttgcatggg
     5761 aaacgctggc gctggatgat gcattggacg ttctggacgc catgctggcc gttatcatcc
     5821 gtgacgccag aaagattggg cagaaaaaac ggctccgctc gctgaaggat ctggataaat
     5881 ctgcattggc gctcgccagc gcatgttcgt acctgctgaa agaagaaaca ccggacgaat
     5941 cgattcgtgc tgaggtgttc agctacatcc caaggcaaaa gctggctgaa atcatcacgc
     6001 ttgtccgtga aattgcccgg ccctcagacg ataattttca tgaagaaatg gtggagcagt
     6061 acgggcgcgt tcgtcgtttc ctgccccatc tgctgaatac cgttaaattt tcatccgcac
     6121 ctgccggggt taccactctg aatgcctgtg actacctcag ccgggagttc agctcacggc
     6181 ggcagttttt tgacgacgca ccaacggaaa ttatcagtcg gtcatggaaa cggctggtga
     6241 ttaacaagga aaaacatatc acccgcaggg gatacacgct ctgctttctc agtaaactgc
     6301 aggatagtct gaggcggagg gatgtctacg ttaccggcag taaccggtgg ggagatcctc
     6361 gtgcaagatt actacagggt gctgactggc aggcaaaccg gattaaggtt tatcgttctt
     6421 tggggcaccc gacagacccg caggaagcaa taaaatctct gggtcatcag cttgatagtc
     6481 gttacagaca ggttgctgca cgtctttgcg aaaatgaggc tgtcgaactc gatgtttctg
     6541 gcccgaagcc ccggttgaca atttctcccc tcgccagtct tgatgagccg gacagtctga
     6601 aacgactgag caaaatgatc agtgatctac tccctccggt ggatttaacg gagttgctgc
     6661 tcgaaattaa cgcccatacc ggatttgctg atgagttttt ccatgctagt gaagccagtg
     6721 ccagagttga tgatctgccc gtcagcatca gcgccgtgct gatggctgaa gcctgcaata
     6781 tcggtctgga accactgatc agatcaaatg ttcctgcact gacccgacac cggctgaact
     6841 ggacaaaagc gaactatctg cgggctgaaa ctatcaccag cgctaatgcc agactggttg
     6901 attttcaggc aacgctgcca ctggcacaga tatggggtgg aggagaagtg gcatctgcag
     6961 atggaatgcg ctttgttacg ccagtcagaa caatcaatgc cggaccgaac cgcaaatact
     7021 ttggtaataa cagagggatc acctggtaca actttgtgtc cgatcagtat tccggctttc
     7081 atggcatcgt tataccgggg acgctgaggg actctatctt tgtgctggaa ggtcttctgg
     7141 aacaggagac cgggctgaat ccaaccgaaa ttatgaccga tacagcaggt gccagcgaac
     7201 ttgtctttgg ccttttctgg ctgctgggat accagttttc tccacgcctg gctgatgccg
     7261 gtgcttcggt tttctggcga atggaccatg atgccgacta tggcgtgctg aatgatattg
     7321 ccagagggca atcagatccc cgaaaaatag tccttcagtg ggacgaaatg atccggaccg
     7381 ctggctccct gaagctgggc aaagtacagg tttcagtgct ggtccgttca ttgctgaaaa
     7441 gtgaacgtcc ttccggactg actcaggcaa tcattgaagt ggggcgcatc aacaaaacgc
     7501 tgtatctgct taattatatt gatgatgaag attaccgccg gcgcattctg acccagctta
     7561 atcggggaga aagtcgccat gccgttgcca gagccatctg tcacggtcaa aaaggtgaga
     7621 taagaaaacg atataccgac ggtcaggaag atcaactggg cacactgggg ctggtcacta
     7681 acgccgtcgt gttatggaac actatttata tgcaggcagc cctggatcat ctccgggcgc
     7741 agggtgaaac actgaatgat gaagatatcg cacgcctctc cccgctttgc cacggacata
     7801 tcaatatgct cggccattat tccttcacgc tggcagaact ggtgaccaaa ggacatctga
     7861 gaccattaaa agaggcgtca gaggcagaaa acgttgctta acgtgagttt tcgttccact
     7921 gagcgtcaga ccccgtatct gttcaaccgt aaggcgaacg gcatcgatga aatctggtac
     7981 atctgccctg accggccgac gcttgtccgg gtacagcggg cgattctggc ggtggatgaa
     8041 atcgtgaacc cgcagaccgg cgaggcccgc aaaacggcgg agctggaccg ggagaggctg
     8101 ttcgcatgct ttaaatttat gacaacggag tgagggatta tggcgctgta catggtattt
     8161 attgaggacg tggaagacaa agacggcgag cgctgcatta tgccaaccag cgagcatctg
     8221 gaagacatgg cggtcatggc ctggcagacc ggtgcatatg tattcaggtt tgaagatcag
     8281 gcccagcatc tggcaatgat gaagtttatc ggcatgtatc acgaacggct gaacagcgat
     8341 gacaaagcca cgtattactt caccggcaaa aatccggcgt gggaacgtga gaaaccggcc
     8401 ggaatgaagc tgtgtgatta tctttatcgc aaggttgagc agttcgccat cgagaacggc
     8461 gtgcaggtac tggacgccgg gaaatacacc ccggagcaga tcggggcgat gattgacgtc
     8521 gtgagtgagc ggtacgggga aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg
     8581 gaatataaca gccgctattt taacgttccg gtgccagaac tgccggagga taaacccgcc
     8641 cgccggaggt ggccgttcgg caaaaaataa acgccagcac cggccatgcc ggtgctgtta
     8701 ttcttccccg agcttatcct gcatcacctg ccagagtgtt tccgcatcct cgtgcagccg
     8761 ctcgcacatg tcggccgggg tatccatatc cagctcgttc agtttctcga gcaacagcag
     8821 cgactggtcc ttaatgaatt tagccatatt gagggcggtt ttttcctgct tcgtcatgtt
     8881 ctccattccc ttcggtaagt tctcagctaa accagccgga ccagaagcgt tttttcttct
     8941 gtgtcagttc tgttgtcaat ctgtccactt cggcctggag tttccggcgc tcatcatcgt
     9001 gctggcggcg ttcttcttct cgggatgttt tatcttccag catcagggtg acagcctgcc
     9061 ggagatcggc cagttccttt tgcattgcca gaagctgttc ctgtgggaaa tttttttgtg
     9121 tattttcacg gtgtgaaatg ccgggaattt gtttctcagg gatattcaca tttccgtata
     9181 cacggatcag ttctgacaca tccacgacag ggttgttttt cccgtcacgt gacacggtaa
     9241 cctttccctg ttttacatga ttgtatagcg ttcttctggt aatgcccgcg gcttgtgctg
     9301 cctgtgaaag gttgagcaat gttttcgcca ctgtataccc ctgattttat cgggtgtgaa
     9361 agatgtgtaa tttctcacgg taaatgtatc gctgaatttt acccgttttt ccgttcagga
     9421 tgtggttttc tgacgcgctg tgcttgtcaa accggcgacg gccacgcaat gaattgcgcc
     9481 cccgtcatcg gttattagag gctgtctgcc cccggatacc cggattcttt ctgatgcctg
     9541 acggtgacga tcacaatccg gtccatttcc cggtcgtggc ggtagagcat cacatagcca
     9601 ctgtcgccaa acccgatcac cagttcctga tattccagcg gcagaaatgg caccgggcgg
     9661 ccaatgtctg gcaatgtttt cagttgctgg atagcccgga cgatcacctc accggctttt
     9721 ctggctgcca gccgattttt ggttttgaga aagtcctgga ggcgttgtaa atctcgctgt
     9781 gccagcgcgg aaatcgttac ctgtggcatg gcggagcatc ctgctcgttc tcagttcccc
     9841 aggtacttat ccaggcttct gcttcttccg cagtaaggtg cagacccgtt tcctgatagt
     9901 gctgccatgc ggcttgtccg tcccgcaggt actggctgcg cttctcttcc cggtcgatgt
     9961 attcggtgat tgcctccagc attagcgcgt gcgctgagcg gtgccggtca tcggccagcg
    10021 ttttcagccg gtccttaagt tcttcatcga gcctgatgga tgttgctgat gacataaaag
    10081 cccctttgta gtcatgtgta atacatatga ctactttaat ccgtggcggc gctatttgcc
    10141 agaaatacgg cttttgctac ccatttttca gcttcgcttc cccatttttc agcttcgctt
    10201 ccactaatca gggctgctac aggctcactc atcatccagg actattgcag cctgcgggtt
    10261 aaaggacctg ttactggcgg caaccatcat cattgaactt tttggtggtg gctggtcgcg
    10321 gatcctgtaa gccgtcattt ttcagactga attcaaaatt tataacctgt cgcattgccg
    10381 ggtgccctgc gggatatagc cagtgcctca ttgccgtcag cgtttcccga aaaaaaagtg
    10441 acagccggta agcgacacgt tgtccactcg tccatcgtcc agaatatcaa acgtggcggt
    10501 cacctgacct tcgatgcgtt ttgctgcggc ctgtaccggg taggttggct gcagtgtggt
    10561 tacgggtttt agtggtatcg catgtaccgg agtgaggaac atggccgcca gcactgtcag
    10621 agtaaaagct ttcatggtct gaaatccaga ggggcgtttt tgaatatttg ccaggtattt
    10681 tcatccggtg ccggcggaat tttggcgtgg gtaacggctg atattgctgc tttacagagt
    10741 ttcggatctc ctccttcagc aatggcgctg ttcagagtgc catcacgttg tatggatatc
    10801 cgcacggtgc acatttttcc cgcataggcc tctggttcaa acatctgcat ttcaacagcg
    10861 gtcttgatgg ccgcagcgta gctactgatt ttagatgact tatccagagt ataaattttc
    10921 ggcggctggg tggtggaatg cttacaggcc gtcaggagta aggggctgca caatgccagc
    10981 aggataaagg gtattaaacg catcgggttg atgacctgtc agtaaaaaat attacatgta
    11041 tatttgccac tctttacggc cacgttcatt gacataaaca cggcttatcg tatcgttttt
    11101 acaccaaacg gcccgccatg cagttgcgtg cataggcatg cagcgggtta caatttaccg
    11161 ggcgcgtttc cggcggtttt ccgggtgatt tgttgcctgt tttatcccgt agccgccgga
    11221 aacgctctga gggcttctcg ggcggtgctg ataacgggtt ttatggtaaa tacccctcac
    11281 cggcggcgca gcggctgcca ggtgaaggtg gtttccgccg cctcgtattt ctcccgacgc
    11341 atttcctgcg cccgcctggc ttcacgttcg tcctgaatgg cgcgaagcac cctttcccgc
    11401 acctgctgtt ttaccgcctt tggcagcgtt ctgttccctt tcaggcccat ggccagtgcc
    11461 tgagcgacaa aggcgtccgc taggcgcttt tcttcagcgg gagtcagttt caggtgtgtc
    11521 atggtggcct ccgggcgtct caggtttcct gaacagttta accgcgggtc ctcccctcat
    11581 accagttagc tttcagatgt gtctatacca cctgaaatgt aagtgtgatg tacatctcaa
    11641 ataataaaat ttgttgccgt gagaaatttt cgggaaaaga cgcgatctgc ttacaggtgg
    11701 ctccgggcgc ttttttgtgc ctgaagatga gaactaatct catatattga cacctgaaaa
    11761 cgggaggagt aaggtaataa ttatactgtg tatatgtaca gtatattggc tttaatttat
    11821 tgttttaaat gtcaaagagg aaaacgatat gagtggtgga gacggtcgag gtccgggtaa
    11881 ttcaggtctg ggacataatg gtggtcaggc cagtgggaat gtgaacggta cgtctggtaa
    11941 aggtggccct tcatcaggtg gtggtacgga tccaaacagc gggccgggct ggggtacgac
    12001 gcatacgccg aatggagata ttcataacta taatccgggg gagtttggta acggcgggag
    12061 taaacccggt ggtaatggtg gtaacagcgg caatcatagc ggtagctctg gtggcggaca
    12121 gtcttcggcc actgcgatgg ccttcggtct gcctgccctg gctactccgg gggctgaagg
    12181 actggcttta tcagtttccg gcgatgcgtt gtcggccgcc gttgctgatg tgctggctgc
    12241 cctgaagggg ccgtttaagt ttggtctgtg ggggattgcg atctacggtg tgctgccttc
    12301 tgagattgca aaagatgatc cgaaaatgat gtcaaaaatt atgacgtcat taccggccga
    12361 tgcggtgacg gagactccgg caagtacttt accactggac caggcgacgg ttcgtgtcag
    12421 acaacgggtt gtggatgtgg tgaaggatga gcggcagcat attgcggttg tcgcaggtcg
    12481 gccaatgagt gtccctgtgg tggatgcgaa accgacaaaa cgtccggggg tattcagtgt
    12541 gtcgattccg ggtctcccgt ctctgcaggt gagcgtaccg aaaggtgttc cgacagcgaa
    12601 agccccgcca aaaggcattg ttgctgaaaa aggtgattca cgtccggctg gttttacagc
    12661 cggtggtaac tcccgtgagg ccgttattcg tttcccgaaa gagaccggac agaagccggt
    12721 ttatgtgtcg gtgacagatg ttcttacccc ggcacaggta aaacagcgtc aggaggaaga
    12781 aaagcgtcgc cagcaggcat gggacgccgc tcatccggaa gaggggctga aaagagacta
    12841 tgataaagcg aaagctgagc tggatgccga agataaaaat attgcgacct taaacagccg
    12901 cattgcatcg acagagaagg cgctccccgg tgcaagggct gctgttcagg aagccgataa
    12961 aaaggtaaaa gaggcagagg caaataaaga tgattttgtg acttataacc cgcctcatga
    13021 atatggctcc gggtggcagg atcaggttcg ctatcttgat aaggatattc agaatcagaa
    13081 tgagaaatta aaagcggctc aggcatcttt aaacgcaatg aatgaatcct tatccagaga
    13141 taaggctgca cttaccgggg cgatggagag ccggaaacaa aaggagaaaa aagcgaagga
    13201 tgcagaaaat aagttaaatg aggaaaagaa caagcctcgc aaggggacta aagactacgg
    13261 ccatgattat catccagtac ctaaaacaga agacataaag ggacttggag agttaaagcg
    13321 aggtgatcct aaaacaccta aacaaggtgg tggtggtaag cgggcccgat ggtatggcga
    13381 taaaaaacgt aaaatttatg aatgggattc ccagcacggt gagcttgaag ggtatcgtgc
    13441 cagtgatggc gaacacctcg gggcatttga tcccaaaacg ggtaagcagg ttaaagggcc
    13501 agatccaaaa cggaacatta aaaaatatct ttgagaggta aatatggggc ttaaattacg
    13561 tttgacatgg tttgataaga aaactgaaga gtttaaaggg gaagagtatt ctaaagactt
    13621 tggtgacgat ggttctgtca ttgaaagctt gggaatgcct ttaaaggata atattaataa
    13681 tggtgagttt gatttgaaga aagaatgggt tcctttacta cagccatttt ttaaaaatca
    13741 aataaaacct gatgtggata attgttatat agcttttgat tatcgggacg gtaactggta
    13801 acaaaaaatt atcaaggatg atttgaaata ctgacttccg gtgttcaggg atgaatgccg
    13861 gagtctttaa atacagggaa agtatatgaa aaaggtaaag actatttttc tttttatatt
    13921 gattgttgct ggctttcttc tggccgcatg tcaggcaaat tatatccggg atgttcaggg
    13981 cggaacggtg gcaccatcgt cctcctctga actgacgggg atcgcggttc agtagaaaag
    14041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa
    14101 aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg
    14161 tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt
    14221 tggcgcacac ttcaagagca acctcgtgtt tagctaaaca aatcctctgc gaactcccag
    14281 ttaccaatgg ctgctgccag tggcgttttg cgtgctttcc cgggttggac tcaagtgaac
    14341 agttaccgga taaggcgcag cagtcgggct gaacggggag ttcttgctta cagcccagct
    14401 tggagcgaac gacctacacc gggccgagat accagtgtgt gagctatgag aaagcgccac
    14461 acttcccgca agggagaacg gcggacaggt atccggtaaa cggcagggtc ggaacaggag
    14521 agcgcaagag ggagcgacct accggaaacg gtggggatct ttaagtcctg tcgggtttcg
    14581 cccgtactgt cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt
    14641 ctgtttatgt gaactctggc aggagggcgg agcctatgga aaaacgccac cggcgcggcc
    14701 ctgccgtttt gcctcacatg ctcgttccct gcttatccac ggaatctgtg ggtaactttg
    14761 cgagagttcg cagcgcccgc cgcagtctca cggccggcgc gtagcgaccg agtgagcgag
    14821 gaggcgcaat ttccccggca cctgcaagac ggtcaccgga cgcgtggatg tgacatattg
    14881 ctccgtgtta ccgcctttct cttccctgcc gataaattat tcagataggt gaagtctgtc
    14941 ggttttctgc tgaatatttt acgttaacct atctctgttg cttttttctc cttgcggaga
    15001 aatgcacgtc atccccgctg gggcgtgact ga
//
