LOCUS       NZ_CP142980             2881 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B19293 plasmid pB19293_9, complete
            sequence.
ACCESSION   NZ_CP142980
VERSION     NZ_CP142980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39499960
            Assembly: GCF_036287785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 01:30:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 60 of 180
            Pseudo Genes (incomplete)         :: 124 of 180
            Pseudo Genes (internal stop)      :: 28 of 180
            Pseudo Genes (multiple problems)  :: 28 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B19293"
                     /isolation_source="seawater"
                     /db_xref="taxon:573"
                     /plasmid="pB19293_9"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            complement(1050..1472)
                     /locus_tag="VYI57_RS29745"
                     /old_locus_tag="VYI57_29750"
     CDS             complement(1050..1472)
                     /locus_tag="VYI57_RS29745"
                     /old_locus_tag="VYI57_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_069346495.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1472..1663)
                     /locus_tag="VYI57_RS29750"
                     /old_locus_tag="VYI57_29755"
     CDS             complement(1472..1663)
                     /locus_tag="VYI57_RS29750"
                     /old_locus_tag="VYI57_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032413520.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2621..2812
                     /locus_tag="VYI57_RS29755"
                     /old_locus_tag="VYI57_29760"
     CDS             2621..2812
                     /locus_tag="VYI57_RS29755"
                     /old_locus_tag="VYI57_29760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     Typing          1887..2380
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.0"
                     /coverage="94.0"
                     /evalue="2.75e-180"
ORIGIN
        1 ggaaccggcg ttcggcaccg ctgaacgatt caattctatt tttgatatta acaggagtaa
       61 tagtgatgag agcattatct cttggtgccg ttttaatggc tatgtcagca ttaccactaa
      121 ccgcgatatc ccaagtaacc ttagcacctg atggatcgta cgttagtggt tcggaagcaa
      181 ccttagcacc tgatggctca tacgttggtg gttcggaagc aaccttagca cctgatggct
      241 catacgttgg aaattaaccc ccattattat gctgcgtacc gatatactga attgcgaatg
      301 ccagcatttt tggatttacc ataacgtctt cttacgcgca ccgctaaaac ggcctcaggg
      361 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
      421 cgcaggctct gtttttacgg catgcatgag tatgcgcgca agtgcatagc gcggaaattc
      481 acgccattca gccagggtaa gtcctgtttc cggcgttctt ctcctccgaa aacggtgaac
      541 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt
      601 cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg
      661 acgactcacc acacgattca ccggcgattc gaatcgtgaa ttgaatcaag agtcaccatg
      721 aacaaaaaac gcccaaattg atgaattgaa tcaagagtcg attcatcata acaacatgat
      781 aataaagcgt gattcacata tgaatcgtgg gcaattcgta aacgattcgc catagagtcg
      841 tccacgactt ggatctgcac tttgtaacac atcactgcac attaattttt cattaaccaa
      901 ttgttttaaa taaggaatct gcaaaatttt aattttgcgt agtgtgtggg tatttttagt
      961 cgtgaggagc gttcagagat ctggcgattt ttgggaaggt tcaggaactc cctgggatgg
     1021 ccggagagga aaactcaagc atggcaggat caggattttt tccagaattt ccaccacggg
     1081 gaatggggtg gtgaagaagg ttcgtgcttg tgctccagaa gctgcatagc ctgacgaagg
     1141 gaatcaatat gctcatctcg agccttaaca gcccctttaa gaccatcgtt ttcagcttca
     1201 agtagagcga ttctatgttg taaatcatgt aaaccaacgc tgttaatact tgtagattct
     1261 gcgtcacttt ttacatcata ttttacagta tcgatgacac catcaagaga accaaacact
     1321 ctaataagct ctgaagtatc aataacttta atagccttcc cgtgctttac aacagaggtg
     1381 taacttaact tcccactgtt aatcatcttg taaattgtgg gacggctaac cttaacaagc
     1441 cgtgccgctt cactaataga cactctggcc atcaattacc atcctgtaat tcatccagtc
     1501 tcgaggacag tgtacggaaa agatgctccg catcatcgtg gagtttctca caaaggtcgg
     1561 cctcaacatc gagatcaagc tcgttaagct tctccagcag gagcagagac tgatgctgga
     1621 tgaatttagc catgttcagc gctgttttct gttgtttcgt cataaagggc ctcagagcgt
     1681 cagtgctcaa tgtcagacag atctcttttc cgcttcctcg ctcactcagt cgctacgctc
     1741 ctgccgttcg gctgcggcga gcggtcgcaa cgcgcacata acgacataga gcagacagct
     1801 cggagaggga gaaggcagca cagcggccgt ttttccatag gctccgcccc cctgacaagc
     1861 atcacagaat ttgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     1921 aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccttt cggtgtaccg
     1981 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
     2041 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     2101 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacacgccaa agcgccactg
     2161 gcagcagcca ctggtaacag gattagcaga gcgagttaat gtaggtgatg ctacagagtt
     2221 cttgaagtgg tggcctaact acggctacac tggaaggaca gtatttggta tctgcgctct
     2281 gctgaagcca gttacctggt taagcagttc cccaactgac ttaaccttcg aaaaaccacc
     2341 tccccaggtg gttttttcgt tttcagagca agagattacg acgagactga atgaatctca
     2401 agatttcatt tagcctgcca cccgatcatt caaagttttg gcgtagttct gacccgctcc
     2461 ccgtgatttg ttatataccc accacggggg cattagtttt tttatttttt ttgatgatat
     2521 gtacaaaatc agtggttagg aataatctta acagccttaa caaaaacaca caaaaacagt
     2581 ggctatgctt tatctcacta cactaagtaa cgacagtatc atgctaaaaa aagatgatat
     2641 tatctttatg aaatcatgtg gtaatgcaaa aacaataata tttttacgtg gcgtggatga
     2701 gaagctcatg attaacgcga gctttgaaga gataaagttt gctatcaata ataatcttca
     2761 ggagcttccg ggggtacaag cagtagccgc gacactgaaa atagcaacct gatttcgagt
     2821 aagggcactc aaaatcaaga gatcaattta ctataacatc ccgatcacga atcccgcggc
     2881 t
//
