LOCUS       NZ_CP142979             3335 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B19293 plasmid pB19293_8, complete
            sequence.
ACCESSION   NZ_CP142979
VERSION     NZ_CP142979.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39499960
            Assembly: GCF_036287785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 01:30:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 60 of 180
            Pseudo Genes (incomplete)         :: 124 of 180
            Pseudo Genes (internal stop)      :: 28 of 180
            Pseudo Genes (multiple problems)  :: 28 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142979.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B19293"
                     /isolation_source="seawater"
                     /db_xref="taxon:573"
                     /plasmid="pB19293_8"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            complement(420..821)
                     /locus_tag="VYI57_RS29720"
                     /old_locus_tag="VYI57_29725"
     CDS             complement(420..821)
                     /locus_tag="VYI57_RS29720"
                     /old_locus_tag="VYI57_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(821..1012)
                     /locus_tag="VYI57_RS29725"
                     /old_locus_tag="VYI57_29730"
     CDS             complement(821..1012)
                     /locus_tag="VYI57_RS29725"
                     /old_locus_tag="VYI57_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049139654.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEELHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1446..>1526
                     /locus_tag="VYI57_RS29730"
                     /old_locus_tag="VYI57_29735"
                     /pseudo=""
     CDS             1446..>1526
                     /locus_tag="VYI57_RS29730"
                     /old_locus_tag="VYI57_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1800..2534)
                     /locus_tag="VYI57_RS29735"
                     /old_locus_tag="VYI57_29740"
     CDS             complement(1800..2534)
                     /locus_tag="VYI57_RS29735"
                     /old_locus_tag="VYI57_29740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_130953829.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGREVSLVNGGFKRFIT
                     GDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKKEAESRSGKWTLSNV
                     KIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(2734..2904)
                     /locus_tag="VYI57_RS29740"
                     /old_locus_tag="VYI57_29745"
     CDS             complement(2734..2904)
                     /locus_tag="VYI57_RS29740"
                     /old_locus_tag="VYI57_29745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771094.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHP"
                     /name="hypothetical protein"
     Typing          1189..1573
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.487"
                     /coverage="89.0"
                     /evalue="2.97e-154"
ORIGIN
        1 ccggcaatca cgttatggca agtcaccgaa cgactcacca cacgattcac cggcgactct
       61 tcactagagt cgtacccaga gtcgccacgc ctaaaaaaca accaaacggg gagtcgggtc
      121 tagagtcggg tcgttattaa ctcattgttt ttatagtcct gagtcgttac cgactctatg
      181 acgactcgcc ggggactcta tgacgagtcg tcatagagtc ggatctatat aaatcaaatt
      241 taaaaaacga aattttacag acttaaacta ctgatattaa atacaaatcc gcaaaatttt
      301 aattttgcgt agtgtgtgga tatttttagt ggtgaggagc gttcagaaat ccggcgattt
      361 tttggaaggt tcaggaactc cccgggatgg ccggagagga aaacggaagc atgacggggt
      421 caggattttt tccagaattt ccaccatggc gccggggatt cagttaattt tgcctggtga
      481 ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt
      541 aagatattca ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc actctggtca
      601 gcagaggctg tatcacgctg ttcggagacc tgtccagaat ggtgttcacc cttcggttgc
      661 tcaaagggac caaagacacg taaaagctct gaagtatcta ccatttttgc attgcgctca
      721 ccactacagg tggacaggtc acctgtttta attaacctgt gcagtgttgt tctactctta
      781 cccgtcagtc gggcagcctc actaatagac actctggcca tcagttacca tcctgtaatg
      841 catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg agttcctcac
      901 aaaggtcggc ctcgacatcg agatcaagct cgttaagctt ctccagcagg agcagagact
      961 gattctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataagaggcc
     1021 tcagagcgtc agtgctcaat gtagggatgt tctcttttcc gcttcctcgc tcactcagtc
     1081 gctacgctcc tgccgttcgg ctgcggcgag cggcaggagc atgcgtataa aagctcgaga
     1141 aaaccgagcg atcgcgtgat ggggagaagg agaggccgca cagcggccgt ttttccatag
     1201 gctccgcccc ccctgacaag catcacagaa actgacgctc aaatcagtgg tggcgaaacc
     1261 cgacaggact ataaagatat caggcgtttc cccctgatgg ctccctcgtg cgctctcctg
     1321 ttccgaccct gccgcttacc agatacctgt ccgcctttct cccttcggga agcgtggcgc
     1381 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1501 ttgagtccaa cccggtaaga cacgactaat cgccattgac aacggccact ggtagacagg
     1561 atgtgcgaga gatgtatcga gagttcttga agtggtggcc tgactgcggc tacactgaaa
     1621 ggacagattt ggtgacatgt ctcgcacaag actgttacca cggttcagaa gttccccaac
     1681 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttccg agaaagagat
     1741 tacgctttga gcgaatgaat ctcaagaaga tcatggcatt tgatgaacaa atgagtaagc
     1801 tactcagatt gatagaaaaa atgcccctta tacaagtgat atattttcca aaactgatac
     1861 tctattttaa cattagaaag cgtccatttc ccactccgcg attcagcctc ttttttaata
     1921 tcagcaatac ttgggatcac cccatttgaa ataacaaaat cgtccaacca atcccacatt
     1981 tctcgccgag atgatttaaa tacaacagga gctttcccat atgagcaagt tggcataaaa
     2041 tcccccgtaa taaatctttt aaacccccca ttcacaagag aaacttctct tccattacca
     2101 ttaccattac cattaccatt tttcatcgta gagctatcag gttccttaat tgtcacactg
     2161 ccatcaggcc caacaaatat ccagtgtaaa ttcaatttac taacagcaag aatgctcgtt
     2221 tcttctatta atctttcata ctgttttatt cttggaacag caatagcata attaatatta
     2281 tcttttgtca ttctctgcac taattgtcca agacaagttc gaaaatccaa ccccggctgt
     2341 gatgtttttc cttttacttc aatatgccac acaacatccc tagattcttt atcacgacaa
     2401 ataaggtcaa tataagaacc cgtatctata tcgaaccctt gtcgaaaaaa atatttttct
     2461 aaagaagctt gaacaaattc ctcggaggga tattcaccac ctagtaagta ccttctcgtc
     2521 accataagag ccacaacccc tacgcatcct atttactata acgtcatatt ttaagagttc
     2581 ccacaagggg aactctctgt acgattacga gtgatcattc gatatatttc gttgaaatcc
     2641 ataccatgaa tcaacatctt cgccgccaca tagcctttct ctaatcaatt tgaggaaagt
     2701 atccctagcc acagctgatg ctagaaaaag agcttatgga tgatgattag agctcacgac
     2761 ccaagcatca ccccgaccac cctcaacaca ctgatacaac cccatatcac ttacattctg
     2821 aacggaatca taaagagcca taacctcctc ataatcgcca tcaacatcat ctaaccacgc
     2881 atcaaaatta gtagcagtcg ccatgccaat cctccatttt ttagttttca ggagttaatt
     2941 tacatagtga taggggctgt taagcaaata agtttttacc ttttttgcct catgctctaa
     3001 aagtgagcaa gacaggcatt taccataacg tcccattgta cgcaccgcta aaacggcctc
     3061 agggcgtttc tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga
     3121 aacgcgcagg ctctgttttt acggcatgca tgagtatgca cgcaagtgca tagcgcgaaa
     3181 attcacgcca ttcagccagg gtaaatcctg tttccggcgt acttcccatc cgaaaacggt
     3241 gaacggggac gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg
     3301 ttttcagcac gttcaggaat ccggttcgtc gacgg
//
