LOCUS       NZ_CP142978             4678 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B19293 plasmid pB19293_7, complete
            sequence.
ACCESSION   NZ_CP142978
VERSION     NZ_CP142978.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39499960
            Assembly: GCF_036287785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 01:30:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,648
            CDSs (with protein)               :: 5,648
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 60 of 180
            Pseudo Genes (incomplete)         :: 124 of 180
            Pseudo Genes (internal stop)      :: 28 of 180
            Pseudo Genes (multiple problems)  :: 28 of 180
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142978.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B19293"
                     /isolation_source="seawater"
                     /db_xref="taxon:573"
                     /plasmid="pB19293_7"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            complement(join(3951..4678,1..829))
                     /locus_tag="VYI57_RS29690"
                     /old_locus_tag="VYI57_29695"
     CDS             complement(join(3951..4678,1..829))
                     /locus_tag="VYI57_RS29690"
                     /old_locus_tag="VYI57_29695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_048293778.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYALKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHSLFSDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KEINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(903..1169)
                     /locus_tag="VYI57_RS29695"
                     /old_locus_tag="VYI57_29700"
     CDS             complement(903..1169)
                     /locus_tag="VYI57_RS29695"
                     /old_locus_tag="VYI57_29700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048293777.1"
                     /translation="MKHTGSRSGQRSIIKRWSWNDRSGSGRMTVRRCNKPDKTGLSAGI
                     CAQSLIHSPGRMAMHSGEIFQAQAARFAGEFTAGFSGLTPHSR"
                     /name="hypothetical protein"
     gene            complement(1176..1391)
                     /locus_tag="VYI57_RS29700"
                     /old_locus_tag="VYI57_29705"
     CDS             complement(1176..1391)
                     /locus_tag="VYI57_RS29700"
                     /old_locus_tag="VYI57_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_048293776.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLSWLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1398..1889)
                     /locus_tag="VYI57_RS29705"
                     /old_locus_tag="VYI57_29710"
     CDS             complement(1398..1889)
                     /locus_tag="VYI57_RS29705"
                     /old_locus_tag="VYI57_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_048293775.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQML
                     DNYMSIREQKDALEKLNARTWGVTYQESSDGRRFLSMPKGTEPQIIPYEGTNWVLLKQG
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1924..2628)
                     /locus_tag="VYI57_RS29710"
                     /old_locus_tag="VYI57_29715"
                     /pseudo=""
     CDS             complement(<1924..2628)
                     /locus_tag="VYI57_RS29710"
                     /old_locus_tag="VYI57_29715"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2567..2890)
                     /locus_tag="VYI57_RS29715"
                     /old_locus_tag="VYI57_29720"
     CDS             complement(2567..2890)
                     /locus_tag="VYI57_RS29715"
                     /old_locus_tag="VYI57_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_177897834.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3006..3178)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.276"
                     /coverage="100.0"
                     /evalue="9.21e-81"
     Typing          3103..3655
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.46"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(1073..2580)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.142"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caataagaat agtagtcaca ctgtcagcac gactataaac caacacattc caagcagtat
       61 ttttcatact agcataacca agaagatatc tatgccccga agaaaaaaat gccccctcac
      121 ctctatcaga cgcagttaac tttgatttca aatcatcaga cataaccata ggccgatcaa
      181 cagcactttt atcgttatta aagatcacat tcccatcatc actaacaatg ctaatttcag
      241 tgttgttctt gaaaaaatgc ctgctcaaaa catcgctaaa taaactatgt ttttttagat
      301 atatagaccc accgacatac cctatgtaat tgccaccctt atcataaata ggatgtgaca
      361 acaaaataat caaattccca gtaactgatt tgaacggtga agatatgaat ggtttctttt
      421 cctcaatcgc caatttgctg gcacttgaat tcaagcgggt accaactaaa tcaagagatt
      481 ctggcgaagt cgccagaaca acagcatcat tactaacaac aaaaactgaa ttaaacatac
      541 cagactgcaa tcttaaccta tccgcctcat ctttaagtga gtcaatatca ttagttgatt
      601 ttattttttt tgctccatat gctaattcac cttgagccat ttcaatataa ccactcatta
      661 catctgacat tttcaaagcg tacgatgaat tagcataaag caaatctttt tctatatttt
      721 cgcgctgaaa aaccataata acggcaatca gaaaaataga agttaacaaa atcccaccaa
      781 cggacaaggt tatgactatc ggacgaaggt gaggcttttt aattctcata aaaatacact
      841 ccatataata aaaaatgccg catttaccat aacatccctt gcccgcaccc ccgcagggcc
      901 cttcagcgcg aatgcggcgt cagcccgcta aaaccggcag taaactcccc ggcaaaccgc
      961 gcagcctgtg cctgaaaaat ttcacccgaa tgcatagcca tgcggccggg tgaatggatc
     1021 aggctttggg cgcagatgcc cgccgaaagc cccgttttat ccggtttgtt acagcgacgg
     1081 accgtcatac gtccgctgcc gctgcgatcg ttccagctcc agcgctttat gatgctgcgc
     1141 tgcccgctcc tgctgccggt gtgcttcaat cgcgttcagc ctttcgacaa ccagcttagt
     1201 tgctcgctca aactctgcac ctgcctgctc aagcccgtga cgcgctcgct cagcgccgcg
     1261 ttctcccgtt gcataagacc agacatcgtc cgccattccg cgaaggcgtt ctcccactcg
     1321 tccagccttt tcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgtttttcc
     1381 agctctgtca tacacagtca cccctgtttc agcagaaccc agttcgtccc ctcgtaggga
     1441 attatttgcg gttccgttcc tttcggcatg ctcaggaacc ggcggccgtc gctggattcc
     1501 tgataagtca cgccccacgt tctcgcgttc agcttctcca gagcgtcctt ctgctcccgg
     1561 atgctcatgt aattgtcgag catctgctgc ccctgccacc acagaatgcc cgcgctcgac
     1621 gctatcagca gcgcggacac caggacgatg gtcagccacg tctggctgac catgcgcacc
     1681 atgcctttcg tgcgctggct catggctgag gacagcttcc ggtcgtggtc gaggatggcg
     1741 gcgctgattc tcttctcgct ttcgttcagt tccgctctga cagacttctc gtgctcgctg
     1801 aatgcggctt tcagcatctc gccggtagtc tgctgctgcg ctttcgattt ctgctctaag
     1861 tccttcgcca gcgttaaaag actgttcata gatggctccc ttcagtcgga tgtttcgccc
     1921 cccgtccggg tcggcaatgc tgatgctgct tttcgtggtg cgcaccacct cgaaacctgc
     1981 gttttccagc gcctctgtaa cgtcctgccg cgttttcagc tccccggatg aggcaagggc
     2041 aagcagaccg cgcgtaatcg cctgggctgc ctcctgcttc gcttccggca gcgcggaagg
     2101 cgtgaccagc gcccgccggt tttccggcgc gttcgggtcg tgcagcccca gcctgccgtt
     2161 cactatggtc tgccaggcat caatgcgcgg acggtcggcg cggtcgtaat acggctggag
     2221 acgtctgccg gtcagcagct ccgtgttcgg gatcaggaag ttcagctcca gacgcccttt
     2281 gtcctggtgc tcaacccaca gcacgctgta ctggtcttta tcgagtccgg gcatcagaac
     2341 ccgctcaaag ctcgccatca gcttttcgcg ctgcccgggc ggcaaatcct gctcggcaaa
     2401 ggacaggacg ccggatgtgt actttttggc gtaaggcgag gcgtcgataa gctcacggac
     2461 ttcctccggc ttcccctgca gaacgctggc gccgtcgcgc tgccggtctt ttcccagcag
     2521 ataatccacc ggaccggcgc caccgccgcg ccctctggga tgaaatttaa cgatcatcat
     2581 cagccccctt ttccagcacg gcgtgccgca gccgctcgag tccggcatca atggccatca
     2641 gtgcggcgac aacctgcacg cgatcgtgcc cggtaccgcc accggcatta acctgccggg
     2701 caatctggtt gaggttgtta cccatgccgg cgagctgacg aagcagcgcc ggcgagatcg
     2761 aaggaagttt gccggcacga gcgggcttct cgtccaggca cgtctggcgc atccaggctg
     2821 cgagctgttt accgtcgcag cgctccagca gtcgacggtg ctcatcctcg gtaacccaca
     2881 tcgtgagcat tttgctgcgt ttgtctgcca atccggcctc ctgataacac cgtgccgacc
     2941 gggaaatctt ttcccggtcg gcacggtgtt ggacaagccg caggcgcgtc agtggctttt
     3001 agcgggtttc ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc
     3061 ttactatgtt ggcacggatg cgagtgtcag tgaagtgctt catgtggcag gagaaaaatg
     3121 cagcagcgga gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     3181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtacc ggcttacagg cggggcggaa
     3241 atttcctggg agatgccagg aagaagctta acagggaagc gataaggcca cggcgaagcc
     3301 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     3361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctcgt
     3421 gcgctctcct gttcctgcct ttcggtttac cgatgtcaat ccgctgttat ggccgcgttt
     3481 atctcattcc acgcctgaca ctcggttccg ggaaggcagt tcgctccaag ctggactgta
     3541 tgcacgaacc ccccgttcag cccgactacc acgttgtccc ggtaactatc atcttgagtc
     3601 caacccggaa cagacacgaa aaatcgccag tggcggtagc cattagtaag aggattcacg
     3661 gagcggagta gtagagtgtt ctacagagtt cttgaagtgg tggcctgact gcggctacac
     3721 tggaaggaca gttttggtac ctgcgctccg tgaaagccag ttaccttcgg aagaaagagt
     3781 tggtagctct tgattccggc aaacgaacca ccgttggtag cggtggtttt tttgtttgca
     3841 gggcatgaga ttacgacgac atcgaaggga taacaagagg atccgcttct tgaacccccc
     3901 tccccacaaa aaaaacggca cactagcgcg taaagcgtca tcaaaaaaac tcaaatagcg
     3961 ctatatacaa tcactttatt acgcccctct ctcttagctt gataaagagc atcgtcagca
     4021 tttttcagca aaaaatcgat cccaccaaga ggatcatctt gtgttgccac accagcagat
     4081 acggtgacac gcaagttatt agggaaaaca gtacgttcaa taacactcct aatacgctcc
     4141 gcaacacact ccgctgttac cacagatgta ttcggcagaa aaacaacaaa ctcttctcca
     4201 ccaaaccgac aaataatatc ttcactacgg caacaagact taataatctg agccaaagaa
     4261 acaagcacct catctccgac cccatgcccg aaactgtcat ttatctcttt aaaaaaatca
     4321 acatcaatag caataactga agctcctgcc ccatacttgt tattctgtat tttagttgaa
     4381 aaccccaatc tattgttcac gccggtaagg ggatctttaa gcgccacctc atttaaagaa
     4441 tcaacccttt ccatcattac cttaacatgc gtaaaaagtg cctttttaag tcgatcagct
     4501 tcggcatacc aagcattgat agctttaata taaactagcg aagctcctat atctttagca
     4561 ttggtagata tagcaagctt ttctaaagga agtgaaatac tggaggcaat gaaatatgaa
     4621 ataagtgaaa acacaataat tattataatt agcagaacaa gtacactttt agcataag
//
