LOCUS       NZ_CP142968             9294 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1906522 plasmid pKP1906522_3,
            complete sequence.
ACCESSION   NZ_CP142968
VERSION     NZ_CP142968.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39501656
            Assembly: GCF_036287765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 01:21:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,691
            CDSs (total)                      :: 5,566
            Genes (coding)                    :: 5,392
            CDSs (with protein)               :: 5,392
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 92 of 174
            Pseudo Genes (incomplete)         :: 97 of 174
            Pseudo Genes (internal stop)      :: 31 of 174
            Pseudo Genes (multiple problems)  :: 38 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142968.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1906522"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP1906522_3"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            complement(join(7566..9294,1..236))
                     /locus_tag="VYI59_RS28355"
                     /old_locus_tag="VYI59_28360"
     CDS             complement(join(7566..9294,1..236))
                     /locus_tag="VYI59_RS28355"
                     /old_locus_tag="VYI59_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1717..1866)
                     /locus_tag="VYI59_RS28360"
                     /old_locus_tag="VYI59_28365"
     CDS             complement(1717..1866)
                     /locus_tag="VYI59_RS28360"
                     /old_locus_tag="VYI59_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1951..2208)
                     /locus_tag="VYI59_RS28365"
                     /old_locus_tag="VYI59_28370"
     CDS             complement(1951..2208)
                     /locus_tag="VYI59_RS28365"
                     /old_locus_tag="VYI59_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(2218..3903)
                     /locus_tag="VYI59_RS28370"
                     /old_locus_tag="VYI59_28375"
     CDS             complement(2218..3903)
                     /locus_tag="VYI59_RS28370"
                     /old_locus_tag="VYI59_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4306..4725
                     /locus_tag="VYI59_RS28375"
                     /old_locus_tag="VYI59_28380"
     CDS             4306..4725
                     /locus_tag="VYI59_RS28375"
                     /old_locus_tag="VYI59_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5031..5294
                     /locus_tag="VYI59_RS28380"
                     /old_locus_tag="VYI59_28385"
     CDS             5031..5294
                     /locus_tag="VYI59_RS28380"
                     /old_locus_tag="VYI59_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5284..5583
                     /locus_tag="VYI59_RS28385"
                     /old_locus_tag="VYI59_28390"
     CDS             5284..5583
                     /locus_tag="VYI59_RS28385"
                     /old_locus_tag="VYI59_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5645..6022)
                     /locus_tag="VYI59_RS28390"
                     /old_locus_tag="VYI59_28395"
     CDS             complement(5645..6022)
                     /locus_tag="VYI59_RS28390"
                     /old_locus_tag="VYI59_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            6091..6270
                     /locus_tag="VYI59_RS28395"
                     /old_locus_tag="VYI59_28400"
     CDS             6091..6270
                     /locus_tag="VYI59_RS28395"
                     /old_locus_tag="VYI59_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6298..6828)
                     /locus_tag="VYI59_RS28400"
                     /old_locus_tag="VYI59_28405"
     CDS             complement(6298..6828)
                     /locus_tag="VYI59_RS28400"
                     /old_locus_tag="VYI59_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6835..7566)
                     /locus_tag="VYI59_RS28405"
                     /old_locus_tag="VYI59_28410"
     CDS             complement(6835..7566)
                     /locus_tag="VYI59_RS28405"
                     /old_locus_tag="VYI59_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     BGC             1..8903
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1036..1614
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6839..7566)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ggccgtttat tatcgcgctt atagcgatcg gcatactccc gtcgctcctt atgactcagc
       61 cccacaaaac gcatccgtcc ggcccaaagt tcagccagac cgaaaaacag gccgataact
      121 aggaaagccc cggccagacc gtaatactcc tggtacagcc agctgaccca cggataaatc
      181 tgccgggcca gctccggaat gtgccacttc caggcaagcg aatccgtatt aaacatgttc
      241 tctctccttc agacgatggt ttgccggcgt gatgtgcagc ccccgctgca caagccgcgg
      301 acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc
      361 acggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag
      421 cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg
      481 gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt
      541 gctgcttcgc caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg
      601 acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacggagc cagaaagagg
      661 gggagagacc gtgcgtcggt ggcgtccagc cagcgtaagc aggggatatc cccctgaggt
      721 cagccacgcc ctcgcgggga tgacctgtat ttctccgtaa ggagaaaaaa gcaacagaga
      781 taggttaacg taaaatgttc agcgaaaaac cgatatgatt cacctatcct ggtaatttat
      841 tatcagggaa tataaagact taatcagggg acaatatgcc acatcagcgc gtccggtgtc
      901 cgtcttgtag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccggcc
      961 gtgagactgc ggcgggcgct gcggacacat gcaaagttac ccacagattc cgtgaataag
     1021 caggggacta acatgtgagg caaaacggca aggccacgca ggtggcgttt ttccataggc
     1081 tccgccctcc tgactgagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg
     1141 gtggctcaac catgaatcag tcaggagtgg cgaaacccga caggacttaa agatcatcac
     1201 cgtttccgat gaatcgctcc ctcttgcgct ctcctgttcc aaccctgccg tttcccggat
     1261 acctgttccg cctttctccc ttgcgggaag tgtggcgctt tctcatagct cacactctgg
     1321 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga accccccgtt
     1381 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaaagcac
     1441 gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     1501 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     1561 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     1621 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
     1681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     1741 ttcagaggag gacgatggtg ccaccgttcc accctgaaca tcccggatat agtttgcctg
     1801 acatgcgacc agaagaaagc ctgaaaaaat caatataaaa agaaaaacaa cctttacctt
     1861 tttcatatac cttccctgta tttaaaaacc ccggtattca tccctgaaca ccggaagtct
     1921 gtatttcaaa tcatccttga taacttttgc ttaccagtta ccatcccggt aaacaaagga
     1981 tacaaagtaa tcaaatttac taatatcgat tacattttta aagtgtggct gtaatatcga
     2041 aacccatgat ttttcaacat caaaccaacc attattaata ttatccttta aaggcatccc
     2101 cagactttca atgacagaac catcatcacc gaagtctttt gagtattcac cacctttaaa
     2161 ctcttcggtt ttcttatcaa accaatgaat atgtaattta agccccatat ttacctctta
     2221 aagatatttt ttaatgtttc gttttggatc cggcccttta acctgcttac ccgtttttgg
     2281 atcgaatgcc ccgaggtgtt cgccatcact ggcgcggtac ccttcaagct caccgtgctg
     2341 ggaatcccat tcataaattt tacgcttttt atcaccatac catcgggccc gcttaccacc
     2401 accaccttgt ttaggggttt taggtttacc ctctttcaac tctcccaacc ccttaatatc
     2461 ttcagtcttg ggatcaggaa aataatcatg gccgtaatct ttagttccct tacgaggttt
     2521 tttcttttcc tcatttaatt tattttctgc atccttcgct tttttctcct tttgtttccg
     2581 gctctccatc gccccggaaa gcgcagcctt atccctggat aaggattcat tcatttcgtt
     2641 taaagatgtc tgggcagctt ttaatttctc attctgattc tgaatatcct tatcaagata
     2701 gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc
     2761 atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc
     2821 accggggatc gccttctctg tcgatgcaat gcgactgttt aaggtcgcaa tatttttatc
     2881 ttcagcatcc agctcagctt tcgctttatc atactctctt ttcagcccct cttccgggtg
     2941 agcagcgtcc catgcctgct ggcgacgctt ttcttcctcc agacgctgtt ttacctgtgc
     3001 cggagtaaga acatccgtca ccgacacata gaccggtttc tgtccgctct ctttcgggaa
     3061 acgaataacg gcctcacggg agttaccacc ggccgtaaaa ccagccggac gtgaatcacc
     3121 tttttcagca ataatgcctt ttggcggggc tttcgcggcc ggaacacctt taggtacgct
     3181 cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg
     3241 tttcgcatcc accacaggaa cactcattgg ccggccggtg acaaccgcaa tatgctgccg
     3301 ctcgtccttc acaatatccg ccacacgttt agtgacgctg accgtcgcct ggtccagcgg
     3361 cagggagctg accggcgtct ccgttaccgt gtctgccggc aatgacgtca cgatttttga
     3421 catcattttc ggatcatctt ttgctatctc agaaggcagc actccgtaaa tcgcaatccc
     3481 ccacagacca aacttaaacg gacctttcag ggcagccagc acatcagcaa cagcggccga
     3541 caacgcatcg ccggaaacgg ataaagccag tccttcagcc cccggagtgg ccagggcagg
     3601 cagaccgaag gccatcgcgg tggccgaaga ctgtccgcca ccagagctcc cgctatgatt
     3661 gccgctgtta ccgccattac caccgggttt actcccgccg ttaccaaact cccccgggtt
     3721 atagttatga atatccccgt taggtgtatg cgtcgtaccc cagcccggcc cgctgtttgg
     3781 atccgtacca ccacctgatg aagggccacc tttacctgat gtaccgttca cattcccact
     3841 ggcctgacca ccattgtgtc ccagacctga attacccgga cctcgtccgt ctccgccact
     3901 catatacttt tcctctttga cttttaaaac aataaactaa aataaaatac tgtacatata
     3961 accactggtt ttatatacag tacaaaaatt acgctgcctt gtttttcatg tcaagtctgt
     4021 aaatttgcgt ttttgtgaaa atgatcaaaa aaacagccac tcacaccatg cgcaatcccc
     4081 tccagatgca acaaaaacca gcacaaacgg cgccgttctc cggcctcagt atgttttact
     4141 gaaatcatta tatgagatac cagaaagccc cctccgggac aggaaaagaa acggggggtc
     4201 tggttcgggt tgttcggtca ctgaacacgg tcagaaactg atgataagat agcctgcgga
     4261 tatataactc aatgagaaaa aagaattaaa ggtaaggaga cgctcatgag acgttccggc
     4321 agcaaactgt gtcttatcgc catgataaca gcgacagcaa ctctcactgc ctgtgctcca
     4381 aagggcagcg tggaacagca tacccggcat tacgtatatg cttcagatga cggttttgat
     4441 cccaattttt ccacccagaa agctgacaca acacgaatga tggtgccttt tttccggcag
     4501 ttctgggata tgggcgcaaa ggataaagcg accggaaaat cacggagtga tgtgcagcaa
     4561 cgcattcagc agtttcacag tcaggaattt ttaaactcac tccaaggcac aacacaattt
     4621 gcgggtaccg attaccgcag caaagacctc acaccgaaaa aatcaaggct actggctgac
     4681 accatttcag ctgtttatct cgatggctat gaaggcagac agtaagtgga tttaccataa
     4741 cgcgtcatta cgcgcaccgc tcagacgggc tcagggcgtt tccggcggct acgggataaa
     4801 acaggcaaca acccccccgg aaaactgccg gaaacgcgcc cgataaattt tatcctaatg
     4861 catgcctatg cacgcaactg catggcatat gttttgacgt aaaatggatg ccgtaagccc
     4921 ttttttaccg actttctgct tcatcagcct gactgacacg accatattct ttcagaccgc
     4981 cccctgctaa acttccttca tattgccgca ttaacccggg aggctccgca atgagcaaca
     5041 ctacacacta cgaaaacgcc aactttcttc gggaactggc tgaaagcctg ccccgcatac
     5101 tgccggaagg tggcccggac aaagctgcgc tgttgcagcg actggctaac gaagaactgg
     5161 cccaggcaga gtatgaagat caggttcgcg ccaaagtgac cgccgcccgt gctgacacgc
     5221 gtccgggtat gaccacggaa caactccgcc agcgcctgca tggccgttat caggaactgc
     5281 gcgatgccgt atgacgtgga atggaagcag ggagccatcg aggatgtaac gacgctgttt
     5341 gactatatcg cggaaaattc atccctgtgg gatgcgcaga acgttaccga ccgggttctc
     5401 gctgcggcgg acaaactggc cgacttcccg cgactctatg aagtggactc acgctatggc
     5461 gaggacgttc gccggatcag tctgatggga cagcatgtcc tctatgatgt tgatgaccag
     5521 accagaaaag tccgggttct ggccgtggtc ggccagcgcc agaatcccca tattgttctc
     5581 taataaccgg tgacggggac gcaattcatt gcgtggccgt cgccggttgg acaagcacag
     5641 cgcgtcagcg ttttccccac ggccaccacc ggcgcggtgt tccggccggg gtctctgctg
     5701 tctccggttt gtgttccagc agtctcatgg actgcatttc ctgccgtagc ccggccacct
     5761 cctccctgag tgcttttatc tccgcgatca acgcgtcgga ggccattttt tcagtctcac
     5821 ccgacagtct caactcgtcc cgctcgtgag tctcatcctg tcttaactca ccgtacaccc
     5881 ggataagctc gctggtatcc accacccggc cgccatcagc ttccgtacgg tatgatacgc
     5941 ggcctttcgc catatcccga tacagggaac ttcgggcttt acccgtcagt tccgtcgcct
     6001 gtctcaccgt cacccacgcc attgtctcac cccgctattt ttgagacagt atacctgaca
     6061 ggcgcacacc atgcccggaa ggaaagaatc atgacaaaac aggaaaaaac agccctcaat
     6121 atggctaaat ttattaagga gcagtcgctg ctattgctcg ataaactgaa tgagctggat
     6181 atggataccc cggccgacat gtgcgagcgg ctacacgagg atgcggaaac actctggcag
     6241 gtgatgcggg ataagctcgg ggaggaataa cagcaccggc atggccggtg ctggcattta
     6301 ttttttgccg aacggccacc accggcgggc aggtttgtcc tccggcagtt caggtaccgg
     6361 cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac
     6421 cgcgctttcc ccgtgccgct cactcaccac gtcaatcatc gcgttaatct gctccggtga
     6481 gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccaccttgcg
     6541 atagaggtaa tcacacagct tcattcctgt gggtttgttc cgttcccatt gcggattttt
     6601 accagtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat
     6661 ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt
     6721 ctgccaggcg agcaccacca tatcttccag atgctcgctg gtcggcatga tgcagcgctc
     6781 gccgttccgg tcctccgcgt cttcaataaa taccatgtac agcgccataa tccctcactc
     6841 cgttgtcata aatttaaagc acgcaaacaa ccgttcacga tccagctctg ccgttttgcg
     6901 ggcctcgccg gtctgtgggt tcacgatttc atccaccgac aggattgccc gctgcaccct
     6961 gacctgcgtc gcccggtccg ggcagatgta ccagatttca tcgatgccgt tagctttgcg
     7021 gttgaacaga tacccggaca ccacttcctg ataccggcga cggctcttga cggtgcgctc
     7081 cacctcaaac gccaccgttt taccgtccag ctccgccagg gcgtccggca ctttcatgcc
     7141 tttaagactc agacggtgca gaatgcgcca gccggtggcc cctctggctt ccatatcaag
     7201 gcgcagggat tgcacggcca gccggtgcgg cagatgtgct gccgacaccc ttgacggctg
     7261 aaacggaata acatccgcca gcggttcgtc ctcgtcgaac gagaacgcca cgccatgcgg
     7321 cgtcagcccc cacacgctga cctgacggcc gtccaccgga tatttggtcc ggaccagcag
     7381 ccctttcgcc accaggctgt cgaggaagcg gtagaccggc tgcaccgccg caatccccag
     7441 ccgccgcgca accagctccg ccgtggaaca cagctcttcc cgcaaccagc gcagggtcag
     7501 ccgggttttc tccgcgttcc gcgcctgtcg tttcgcgtgg ctgacgttgt acagctccag
     7561 cgccatcagt aaatccccgc aaaatcatca tccgctttcg gcccgccgcc gtcggccggg
     7621 ggccggaact ccgccagggg atcggttttc gccagcaccg ggaaacgctg caacgggatt
     7681 tcccggcgct caacccgcag cggactgctg aacgccatcc gggccacacc cagcccggtc
     7741 atgaccgcga acccgtccgg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc
     7801 tccttctgga tgaagctgtc cccgctgata tgctccacgt tccccgcctc ccggctggca
     7861 cggcggcgct ccacgtccac cctgatttcc ccggtctgcc ccgccgccca ctgcgcggac
     7921 tccaggctgg ccgcccggta aaaccagcgg atcggcgtgt tatccagtac cgtggttttc
     7981 acaaattcag cgggcagatc ctgcccgcac tgcccgaaat cgcccagcga ctggtgcgcg
     8041 agcaacagat tgcagtcccg gctgcgcagc gtccccagtg cgttcagtac atacttcgac
     8101 agcagatatt taatttcatc gagcatcacg ctgacgtgtg gccactcccg gaactcgtcc
     8161 cgcgcaatga tgatttgcgc gcagcgggca aacagcattt tttgcacacg gatcaccgcc
     8221 tcgtcgtcca tcgagccgat aacgtacagg cagccgccgc cgttgataat ccccgccaca
     8281 tcgatgccac tgtccgtctg caggacgctc aggtcagcga cgttttccag cctagtgatc
     8341 aaccccttca cgtccttttt cagggcctcc ggcagcgcgt acgccgccgc cagcacctgc
     8401 tgaatgtttg ccccctgcgg aaactgctcc gcaatcagct tcgccgcttt ctgctcgctg
     8461 atgcggtagt ggtccgccac gtcgccggtc tcgcccaggt taagcccggc aatcagcagg
     8521 tttttcagcg cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc
     8581 cgcagatcca gcagggtgaa gtccggacag tgcgcccgga agacgtgcgg cgcccatgcg
     8641 tcccctttgg ggtcgaagta caccaccgcg tccccgaaat cgcgtacgca gcgaatgagc
     8701 gcgttggtgg ccatcacgct cttgccgctc ccgggcaggc cgaggatctg aatgttggtc
     8761 ttgcgccagg tatcacgcgg aaccagcacc ggcttgccct gctcatcctt acccagaaac
     8821 accgcctgct cctgccgggc ctgctccgag gaaaaatcgg ccagcgcatt aaccggtacc
     8881 ggctccccga aatgcaggtg gcgcacgtcg gtcatctctc ctgtccgctg gctgctgcgc
     8941 cggctctcct gattcagttt cgcgttcacc gcctcgtcag cagccgcaaa atgaccgtca
     9001 agcagcagga acgccagcag ccgcgccaac acaatcccga cagccagccc gcccgcgcac
     9061 caggcaatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt tgcgtagagg
     9121 tccggacgca ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc
     9181 agaagggcca gaccggccgc gccggtcagc cagtacgcgt agttccagac atgtcgacgt
     9241 gccctgacca tccgcccggg cgaatccagc gaggccagca ccgaaaatgg cgct
//
