LOCUS       NZ_CP142948             4073 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Escherichia coli strain B20159 plasmid pB20159_5, complete sequence.
ACCESSION   NZ_CP142948
VERSION     NZ_CP142948.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39500351
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 285.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/19/2024 10:32:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142948.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B20159"
                     /isolation_source="seawater"
                     /db_xref="taxon:562"
                     /plasmid="pB20159_5"
                     /geo_loc_name="Ireland"
                     /collection_date="2020"
     gene            join(3480..4073,1..1554)
                     /gene="mobQ"
                     /locus_tag="VYI61_RS00005"
                     /old_locus_tag="VYI61_24855"
     CDS             join(3480..4073,1..1554)
                     /gene="mobQ"
                     /locus_tag="VYI61_RS00005"
                     /old_locus_tag="VYI61_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1812..2513)
                     /locus_tag="VYI61_RS00010"
                     /old_locus_tag="VYI61_24860"
     CDS             complement(1812..2513)
                     /locus_tag="VYI61_RS00010"
                     /old_locus_tag="VYI61_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3047..3301)
                     /gene="mobC"
                     /locus_tag="VYI61_RS00015"
                     /old_locus_tag="VYI61_24865"
     CDS             complement(3047..3301)
                     /gene="mobC"
                     /locus_tag="VYI61_RS00015"
                     /old_locus_tag="VYI61_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(3303..3479)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3480..3838
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1813..2513)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3394..4073
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.357"
                     /coverage="100.0"
                     /evalue="1.22e-144"
ORIGIN
        1 cctggtattc acctcggaaa gtctgcctgt gcgatggaaa cacgcggtat cgaaacagaa
       61 cgcggcgaac agaatcgtct gattaacagg cttaacctgg aaatacagat ttcccgcacc
      121 gaactcagga acggagggct gacagagcct gcccgccgtg tggctgacct tctgaatatc
      181 gggcttcctg acaacgcaac ttcgtacaca ctacgtgaca ttatcgaagc gttgccgaag
      241 gacagtaact cggcatggaa actcaccagt aatgccatgt cgatgatggc agatatgcag
      301 gcgacgcgca gacgctggga agagctgaac accgaacgaa aggaagcgat gagccatgct
      361 gccagcctga aaaaatcatc gccgttttcg tccggttttt cccgtattcc cctgatgcaa
      421 tgggcggcac cggaataccg tagtgagcag ggaaaaatcc agtctctgag ccagcaaatg
      481 gaaaaattac gacagcatta ccggaaggtg gagaaacagg acattcccgc gagtcagatg
      541 gcatttgaaa aacagtggca gcagtggggg gaatccggcc ttgcagctct cagagcccag
      601 ttgcttaaac gtgaagagga attgcgccgg aaggaacagg aagatactga acgtagtcgc
      661 gcggagcagc tcaggaaaaa tgacaatgcc gttatggatt caggggtgct gagagcagtc
      721 gttacggggt atggcgaggc tcccatgcct ggtaacgggg aaatgacctg ttaccttctg
      781 cttcacaacc ggaacggaga atatacgctc tgggggaacg agctggagaa ataccgcacc
      841 agaatctttg agagtgttga tttaatgcgt gacaggagtg gttatatctg tgaccgaagc
      901 gaaatcggcc aacgtccgcc actgcaacgt gtttattcct ctgcaacctt tgagcaactg
      961 ctgacgcagg tttgccagac atggccgcaa catacccgca atctccgcca gccgaagaca
     1021 tggccggagt ctttctgtct tggagaagac cgccagccag ccatgccatc accagcggca
     1081 aggaaggtgg attttatgca gggacgtctg ccaccgacgc tgatgccggt catgagttct
     1141 gtcgaccggg aaacccgcca gcttcagctt ttgctggtca tgggcgtgga tgactctctg
     1201 ggcggtgtta tccggcttaa tggtacgctg tatcccgcgt ttgccgttcc ctcagccgat
     1261 aacaggcatc tggtcatcag tgcgctgacc gataaggggt tacggtatgc agggtatggt
     1321 gttgccgtaa atcacgatgc agacagccac atatcacctg caccagaact gatgcagttt
     1381 catctgaaaa cccgtgaggc accgcttttt gctgccgtca acacaccaga aaaacagccg
     1441 gatcatctgt tccgctcgct gggttttaat cgtacctggg aggaatggca gcgcgaggaa
     1501 gatgcacgaa cacacaccac cgagaggaga catgatcgcg gttggtcaca gtgaatgtga
     1561 acaatatttt gttttgttta acatcgtgtt atgttcgtcg atgttcttcg atgttcatcg
     1621 acagggatga atgcaagaag cacactcaca taacgcaagc atagttttgt ttttccgccc
     1681 ccaaaaattg gggggtcttt tttcgctgca tcagagcgtt tttgacgcaa tttttcactt
     1741 caatagtacc ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct
     1801 taaaatcgat tttagatcgt catcttgatc tgtttgtctt ccttgaagcg aaaatgcagg
     1861 gctacaaccg tgcgcccctt tttgatcgtc tcaacagtta caaccagatc gcttttttcg
     1921 ttcagttcgg cgatggctgg cttgattatc atcttattca agtctttgaa ttgttgatat
     1981 ttatcttcaa cggcaagcat ggttcggaaa tcatcgatag ctatcgttct gtcaccggtg
     2041 ctgcggaact gctggagcag ctcgtaaatg cggatgctgt aggtgctgga aaggctggag
     2101 acgttcttca cgactacccg cgtaaactgc cccttgagct gtgtcagata cggcatgatg
     2161 gcatcggaga aggtgatatc tactttcgcc tcacctttga aatactgcgc ccggtactgc
     2221 acccagcgga actcttctcg tttttcctcg ttcttgatga ttactgacct gtcccagagg
     2281 cgatctatcg cttccttgag cctagcttca gcgtgctgcc gtcccatgtc aggaaaggca
     2341 tcatgaaact ctgcagccgt gatggtcatg gttttttgtt catccggcga aggggaatct
     2401 tctccggatt ttaggcggct gatacagacc agcaggaagc gctgttcctg caaagtgagc
     2461 ttgtagcttg cctcgataag ggcgtttgat ttgtaagcta tcagatctga catttcgctt
     2521 gctcgttgct attggtggcg tttttttact gtacgccact tatcatatgg gtggaagttt
     2581 tttttttgca accccctttt tctgtggatt acacccccaa aagctccacc tttcaccccc
     2641 aaaagctcca cctttcaccc ccaaaagctc cacctttcac ccccaaaagc tccacttatc
     2701 ttgccagaac atctttaaaa tcatggtgtt acaagaccta aaaaggtttt aaaaaaaaag
     2761 atctaaaaat aagatcttaa aaacttaacg gcgagcctgt ggataacttg gggtgctgaa
     2821 aacaaaaaac caataaaaac aatgcgttgc aataaagcag aattgcaagt ttcgaataca
     2881 ccccctccaa ccctgtggaa acccagtatt catgcgggtt cagcgcatat cacctgccga
     2941 aaaaccgtgc gtatgtgctc aacctggcga ctgattcact tccgcttcct cgcccactcg
     3001 ctcgctaacg ctcggtcgct ggggctgcgg cgagcggacg atacaatcac tgttctggca
     3061 gcgcctccag ctcgttaatc agcggcagta tgcgcgtcac atcagcctgt cgtgtcacct
     3121 ctgccttcag gaactggagt aaccaggcac gcaactgcac ctcctccggc tttctgacct
     3181 ttgccagcaa tgctgcaccc acaacaacct tctgcgctgt gtccaggctc cggctcttcg
     3241 ccttcaggcg ctgtaatctc gcctcagcat cagcaatctt ctgttcgagc gttctgctca
     3301 tgatgtaact ccgtgcgcga tgaaaaatcg cattttagcg cgtcactggt agtttaaaaa
     3361 ctgagctggc ataatgcacg gcaaatcacg aagtgcgcac ttatacaatc tccactccgt
     3421 ttcgattgtg tgcgccctgc ggggctgaaa gaaaactaca aaaaggcatt acggcagaaa
     3481 tggcgattta tcatctcagc atgaaaatta tctccagaag cagcggctac agcgccgtag
     3541 cttccgctgc ctatcgttcc ggttcactga tgcttgatga gcgtaccgga ctgactcacg
     3601 actacacccg caaaagcggt gtggctgagg ctgtgattct gacccccgcc accgcccctg
     3661 cctggtgtac gaaccgtgcc gaactctgga atgccgttga gaaggccgaa cgccggaaga
     3721 actcacaact tgcgcgggaa attgaactcg ccatccccca cgaactgccg caggatgccg
     3781 cacgggaaac cgttctcgcc tttgtcagag aaaactttgt cagtcagggc atgattgctg
     3841 atgtggcgtt ccatcacatg gataaaacaa atccccatgc gcacatcatg ctgaccacga
     3901 gggctatcgg acctgctggg ttcagcggaa aggtcaggga ctggaacgac cggacacatg
     3961 ccgagacgtg gcgggcatca tgggctgacc atgcaaacag ggcactggcg aacgccggtt
     4021 atcaggaaga gatagaccat cgttcatacg agcgacaggg gcttgagaaa acg
//
