LOCUS       NZ_CP142933             7939 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Escherichia coli strain B20123 plasmid pB20123_3, complete sequence.
ACCESSION   NZ_CP142933
VERSION     NZ_CP142933.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39500237
            Assembly: GCF_036287665.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 307.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 19:50:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,286
            Genes (coding)                    :: 5,021
            CDSs (with protein)               :: 5,021
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 265
            CDSs (without protein)            :: 265
            Pseudo Genes (ambiguous residues) :: 0 of 265
            Pseudo Genes (frameshifted)       :: 92 of 265
            Pseudo Genes (incomplete)         :: 184 of 265
            Pseudo Genes (internal stop)      :: 54 of 265
            Pseudo Genes (multiple problems)  :: 60 of 265
            Pseudo Genes (short protein)      :: 4 of 265
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142933.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B20123"
                     /isolation_source="seawater"
                     /db_xref="taxon:562"
                     /plasmid="pB20123_3"
                     /geo_loc_name="Ireland"
                     /collection_date="2020"
     gene            complement(join(7318..7939,1..971))
                     /locus_tag="VYI63_RS26890"
                     /old_locus_tag="VYI63_26890"
     CDS             complement(join(7318..7939,1..971))
                     /locus_tag="VYI63_RS26890"
                     /old_locus_tag="VYI63_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(1010..1708)
                     /locus_tag="VYI63_RS26895"
                     /old_locus_tag="VYI63_26895"
     CDS             complement(1010..1708)
                     /locus_tag="VYI63_RS26895"
                     /old_locus_tag="VYI63_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(2256..2414)
                     /locus_tag="VYI63_RS26900"
                     /old_locus_tag="VYI63_26900"
     CDS             complement(2256..2414)
                     /locus_tag="VYI63_RS26900"
                     /old_locus_tag="VYI63_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(3073..3264)
                     /locus_tag="VYI63_RS26905"
                     /old_locus_tag="VYI63_26905"
     CDS             complement(3073..3264)
                     /locus_tag="VYI63_RS26905"
                     /old_locus_tag="VYI63_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3329..3652
                     /locus_tag="VYI63_RS26910"
                     /old_locus_tag="VYI63_26910"
     CDS             3329..3652
                     /locus_tag="VYI63_RS26910"
                     /old_locus_tag="VYI63_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3861..>4292
                     /locus_tag="VYI63_RS26915"
                     /old_locus_tag="VYI63_26915"
                     /pseudo=""
     CDS             3861..>4292
                     /locus_tag="VYI63_RS26915"
                     /old_locus_tag="VYI63_26915"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4330..4848
                     /locus_tag="VYI63_RS26920"
                     /old_locus_tag="VYI63_26920"
     CDS             4330..4848
                     /locus_tag="VYI63_RS26920"
                     /old_locus_tag="VYI63_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            4855..5088
                     /locus_tag="VYI63_RS26925"
                     /old_locus_tag="VYI63_26925"
     CDS             4855..5088
                     /locus_tag="VYI63_RS26925"
                     /old_locus_tag="VYI63_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5799..6038
                     /locus_tag="VYI63_RS26930"
                     /old_locus_tag="VYI63_26930"
     CDS             5799..6038
                     /locus_tag="VYI63_RS26930"
                     /old_locus_tag="VYI63_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            6035..6397
                     /locus_tag="VYI63_RS26935"
                     /old_locus_tag="VYI63_26935"
     CDS             6035..6397
                     /locus_tag="VYI63_RS26935"
                     /old_locus_tag="VYI63_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(6422..7315)
                     /locus_tag="VYI63_RS26940"
                     /old_locus_tag="VYI63_26940"
     CDS             complement(6422..7315)
                     /locus_tag="VYI63_RS26940"
                     /old_locus_tag="VYI63_26940"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     Typing          2918..2997
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(2343..2885)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3297..3646
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          3643..5192
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcttgtctg tgtaatttgt tttttttatg tcgtttattt cggcacagta taaatcaatt
       61 tctgttcttg ccatttttat ttgtctaaat agataaccaa tgcgatttaa tgggagaaaa
      121 acttggaata aaaaagtggc aagcataata atactaccac ggcttgctct accatgaaga
      181 aacatccagt atgctgatag gataatcaga ctactaaaaa tgactgaaag aaaagcggag
      241 ctgataccaa acaggaaaaa ttcattattt acttttcttt gtttttctga atgtgtattt
      301 acagcttgaa ttactcgttt acgactttta tccgtggtat ggaaagcttt atccatagga
      361 tacatgtgca tcatatcaaa cattcttcca gatgcgaatt tggagctaag tgcaactgat
      421 ttcatcattg gaaatctacg ttgtgtcatt tttatgacaa atattatata gattgccatt
      481 attattataa actcaatggc tattaatgta ttaacactta tacaaattgt tatgattatt
      541 ccaatcattt gtattaataa tggtactaca tcagaacata ttgtataaaa taatgatgat
      601 gcagaagaaa gcatgctttc tataacctta tttagttctc ctttattttt ctttaaaaaa
      661 tgctcttggt gagaattttg tatattatca ataacttgct ctctaagctt tacagataat
      721 tctatatttg ttttttgagt caagtattcc gatatgctat atccagcagg cataaggaat
      781 ctaattgata agaaacatgc tataaatatt aataatgagt ttatttctcc tattgaatct
      841 ttattgtcaa taactgtttt tattaaatat ccgaatagaa cgaaaagaaa actacttata
      901 gttgataata atataataaa aaacaaatta catttattaa atgtgatgat gtacttttca
      961 ataaaattca atttataatc cttaaagccc gtatattacg ggctttatgt tatctagaat
     1021 tacatgagca cccagcgcca catcctttac cgctgcaacc acaaacagaa ccattatcat
     1081 tccaaacatc ttttcgcatt attattttta caaattctgc ggctggaagg tgagtgatac
     1141 tctttacttt ttcgatgtaa gaatcactgg atatggattc ttttggaatt cttatagtat
     1201 taatgttctt tatgctgtat cttacagcat aatcaccata ccactttgcg acagtttcat
     1261 gacctaggta aaaagcgatt ttttcaattt gatttttata ttcgttaaaa cgatattctt
     1321 ggaatgttat accatgctca ttaacatgcc ttatctcatt ttttagcatg tcatttgtat
     1381 ttggataatg gtggaggtaa gcaccaagat atttttcaca aaagctggta taagcatctg
     1441 taaattgaat aaacgtatgc cacatttcat ctataatttg taattgctcg tgaactttta
     1501 tattgtagca ttccgttagg catgttgagg ctagccagat gaatttcttt gtttcattaa
     1561 agatatcatc agcttcttct ggtgttacat tccattcttt acgaaatttt agaagaacat
     1621 catcattgtg ataactcatg atatcttcta atgagctaac attatgaact tttgcataag
     1681 aactgctgtt catcttatgt tgtagcataa cacccccaat tatatatttg aatgtagatg
     1741 agaatagtta tcatttgtta tggtattgtt aaatttgtgc ttgtgtcaat atgcctaatt
     1801 ctataaatga gatagtaatc aagtttttta atggttatag gatccattag taatacaagg
     1861 tttttgcagc cagtttttac gggtaggggc tgcaaggaaa acaaaatttt cccactctaa
     1921 gagaagagaa ttgagttggt tgaatgagtg gatgcatcta gggccgctaa ttcaccgagt
     1981 gtaaaggctg tacgaaatgt gttgcagtcc gataatagcc ccctccattg acgttttaca
     2041 catgtgttgt aggttccagg agtgggcgga tctcaaaaag atcaaaggat cttcttgaga
     2101 tccttttttt ctgcgcgtaa tctgctgcca gtaaacgaaa aaaccgcctg gggaggcggt
     2161 ttagtcgaag gttaagtcag ttggggactg cttaaccggg taactggctt ggtggagcac
     2221 agataccaaa tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt
     2281 aatatctcaa tccaccttgt ccagttacca gtggctgctg ccagtggcgc tttgtcgtgt
     2341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgagcc gagataccta
     2461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttatgatgc
     2641 tcgtcagggg ggcggagcct atggaaaaac gccaacggcg cagccttttc ctggttctcg
     2701 ttttttgctc acatgtttct tttggcgtta tcccctgatt ctgtggataa ccgcatctcc
     2761 gcttttgagt gagcagacac cgctcgccgc agccgaacga ccgagtgtag cgagtcagtg
     2821 agcgaggaag cggaagagcg ccggaacgtg cattttctcc ttacgcatct gtgcggcatt
     2881 tcacatcgga catggtgcgc tttccataca attcgtactg atgccgcata gttaagccag
     2941 tatacactcc gctatcgcta cgtgactggt tcagggcttc gccccgaaac cccctgacgc
     3001 gccctgaggg gcttgtctgc tcccggcatc cgctcacaga caagctgtta ccgtctccgg
     3061 gagctgtatg tgtcagaggt tttcaccgtc atccccgaag cgtgcgaggc agctgcggta
     3121 aagctcgtcg gcgtggtcgt gaagtgattc acaaatatcg gcctgttcat ctgcgtccag
     3181 ttcgttgagt ttctccagca gcgttaatgt ctggcttctg ataaaacggg ccatgttaag
     3241 ggcggttttt tcctgtttag tcactgatgc ctccgtgtaa gggggatttc tgttttatgg
     3301 ggtaatgata ccgatgaaac gcgagaggat gctcaccata cgggttactg atgatgaaca
     3361 tgcccggtta ctggaacgct gtgagggtaa acagctggcg gtatggatgc ggcgggtctg
     3421 cctgggtgag ccggttgcac gctccgggaa gctgccgaca ctggcaccgc cgttactgcg
     3481 tcagctggcc gccatcggga ataacctgaa tcagacagcg aggaaggtga acagcgggca
     3541 gtggtcttcc ggtgatcggg ttcaggtgtt ggctgcgctg atggccatcg gggaggagct
     3601 gcgtcggctg cgtctggccg tcagggaaca gggcgcgcgg gatgattgtt aaatttcatg
     3661 ccaggggaaa aggcggtggc agtggtccgg ttgattacct gctgggaagg gagcgaaacc
     3721 gggaaggggc aacggtgctt cagggtaatc cggaagaagt ccgggaactc atcgatgcca
     3781 cgccatttgc gaagaaatac acgtcaggtg ttctgtcgtt cgcggagaag gagctgccgc
     3841 cgggaggacg tgaaaaggtg atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa
     3901 agaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt
     3961 ttgtcattcc gaacatggag ctacagaccg gaaaacgcct ccagccgtac tacgaccggg
     4021 cagacaggcc gagaattgat gcctggcaga cgctggtgaa tcaccattac gggctgcatg
     4081 accccaacgc cccggagaac cgccggatac tgactctccc tgataacctg ccggaaacga
     4141 aacaggcgct tgcagagagc gtcacacgcg ggatagacgc tctttatcat gtcggggaga
     4201 taaaaggccg tcaggatgtg attcaggcgc tcacagaggc agggcttgaa gtggtcaggg
     4261 tgatgcgaag cagtatcagc attgcagacc cgaacggtgg gaagaatatc aggctgaaag
     4321 gagcatttta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag
     4381 agagccgaat ctacagagac aatgctgaac gacgagttca gcaagctcga aaaatctgta
     4441 agcaaggctg tgacatcaaa cgagacgaaa atcaaagacg ctatagccct gttcacagct
     4501 tcgacagagg aatcactgaa aaaacaccgg gaaggggtga aagaggcgat gatgcagcac
     4561 aggaaggacg tgttaaagct ggcagggaat acgggcatga tgttactggg gatggtcctt
     4621 ttcctgttta ccgtgagtgg cgggacgctc tggtatcttg gcgggatgat acaggcgaac
     4681 ctggaagaaa tcaggataca ggaagaaaca ttgcagaaac tgaacgcgaa gacatggggc
     4741 gtggagtttg tgcaggacgg gagaaggaaa tttctggtta tccctcaggg gaaatcggca
     4801 acggtgattc cctatcaggg gaaggactgg gtacaactga cggagtgaca cagagtgacg
     4861 gagctggaaa gacatttgct gaacgcctta gagcaactgc aacaggacta tatgcagcgg
     4921 ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg
     4981 gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca gcacttgagc
     5041 gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg gatgaacggc
     5101 tgatacagga acgcgaacat gagttatccc tggagcgtga gcgccagctg gaaatacagg
     5161 aacggacaca ggatggccct tcgctgggat ggtaatcaga gacaggctaa aatgttgtca
     5221 gcgcgtttcc ggtgcatttt ttgggataat ggttggttcg ggggcaccgg gaacgcgctt
     5281 cagtcagacc ttgctgaaaa cacaggaacg cgttaaaacg catacgggcg cgattctggt
     5341 gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc cctgaacttc
     5401 tgttttcccg ctccggtccc cgtcttcttc gcttccgaaa gcatgaaaaa aatccgtttc
     5461 agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta tgcatgtggt
     5521 taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca tttttacctg
     5581 tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaataagg gattatggta
     5641 aattatcttg ttccttagag aatgatttga ggtggtatcc tgcacttatt taaatatgct
     5701 tgcttgggaa attccaggtt gcagtatggt tgagttgtaa ttttcctatg ataggaaaaa
     5761 tgttatatta ttaatttatt tgtgaggctt cataaagaat gaaaatttct gatattgaaa
     5821 aaaacatata taaaggacta gctgaattta tttcacaaaa aatcagagat gaacatgata
     5881 tatgccttgg acagtttgaa tcagaggaat tacttgatga gattcttaaa agagtaatcc
     5941 cagtcatata taatgtggct attgatgatg ctataaaatc agtaaggaat gttggggata
     6001 gattagagga ggaattagat atgaggaagg tagtgtgact aattttaaat cagacttaaa
     6061 tctaataaga aaagaatggt ataatgcatt ttattcaggt aatactgaaa tgctagatta
     6121 cttggaagct gattggttta tttcaacaaa tggtcaaaaa gttatctata aaaaacatca
     6181 gttacgaaag attgatatgc gtccagcgag gattttacct agcatagaaa gatatgaata
     6241 tgatgtcgta atgcgtgagt ttaaaggcat tgcttgtgtt tcagggaaag ctgaaataaa
     6301 gcaaaatgat ggaaatatat taatggcttt tattgagaat tggataaagg ttaatggtgg
     6361 atggaaaatg cagtttatat cgtatgaaaa aatctaaggg gaagccccct tagattaatg
     6421 gttatatatg ttcaagtttt atttttttat agcgataagt tctgctttga gctttaagca
     6481 tgttttctgc aaactgaata tcaaaactat ttatagcatt agttattata gtttttattt
     6541 tttctttatc agataatgaa tcaccatgct ctataggttc ccgcatatat ggattgtttt
     6601 ttaattcatc aaataatgtt ttaaatagtt ctaatcgacg aggtcttata actgtaaaag
     6661 atatatgaag agagtgtttg ctctgagtcg taacattgtg ccagtatcca gcaggaagat
     6721 atatggcctg acctggggtt atatcaaact cataatctgg ggaggtatta ggtggtagat
     6781 aatcgaagct tctttcatct tcgagagggt atgaataggt agggttgtaa actttccatt
     6841 tttttattcc ctcaatttga acagctatta catcatgatt gtcaaaatga agcccaaagc
     6901 ttggtgttgc tgtaaatgca gcatatagat tagcactaca tgtacattct aactctttag
     6961 ataaccatag tctgctttca tctacagatt caaagaatga ctggcatcga tctactataa
     7021 gggttgcgcc atcacgtaag catttatata gctgatggcg atttatatgt ggtgttctat
     7081 cgcctgtttg gctatatgtg tatcttataa atcctttata ccccctaatt tcgggaaagt
     7141 tgtcatttgc cattctaact ctaggatagt gcaatatatc tttttcaagc aatttattaa
     7201 gatcattcca gttgattaag tttttataat taacatccgt gaatacaaac ggatatttct
     7261 ggaaattata tttcttaaat ttttctatga tttccttttt tataagatta ttcattttta
     7321 aacctcatgt tttgtgatat ctataatctg tgctttaggt atattattta taagttgttc
     7381 atcatgagat ataacaatga tatgcttttc cttgttttcc tcttttatat aatcactgat
     7441 tatttttata ccaagtgaat caaacccttt tgtcggttca tcgaaaataa tcaagtctac
     7501 gttttttcca gatgtaagag atactcctaa tttttgtttt tgtccaccag ataaattatg
     7561 tccgtaacct ttgatctcat aatctagtgg tttgtcgaac ttaaatctgt gtagcagctt
     7621 tttaattgta tttaaattat taatgccgga ataatgtaat atattttcct ctatagttcc
     7681 aggcatgaga tctagatctt gaggaacgta taagacatta acaaaaggag aatttgcttt
     7741 ttcttgttgt atcccattga tttttattgt gttttcttct gttgttatat tgccagagag
     7801 aatttttgca atagttgttt ttcccgaacc attctcaccg gttataaagg taacaacgcc
     7861 ttttgttaat attaatttat ggttgaatga tttgttgtat atttcaatat tgcaaatatt
     7921 gtcttttaca tgaagatat
//
