LOCUS       NZ_CP142929             1783 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Escherichia coli strain B20105 plasmid pB20105_4, complete sequence.
ACCESSION   NZ_CP142929
VERSION     NZ_CP142929.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39500209
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 299.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/19/2024 10:29:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1
            CDSs (total)                 :: 1
            Genes (coding)               :: 1
            CDSs (with protein)          :: 1
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142929.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B20105"
                     /isolation_source="River estuary"
                     /db_xref="taxon:562"
                     /plasmid="pB20105_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2020"
     gene            1..990
                     /locus_tag="VYI62_RS00005"
                     /old_locus_tag="VYI62_26015"
     CDS             1..990
                     /locus_tag="VYI62_RS00005"
                     /old_locus_tag="VYI62_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001529700.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEILNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(717..908)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 atgaaaaatc ctgaactcca gaacctcacg gactattccc cctcagatgc gccctgggac
       61 gctcacagaa gcgtttctga cgacgtggga gggatttacc tgcttgcggc cgaatatgag
      121 cgctacgggg cgcgtatggc cctctgtggc ggtttattgc gtttcgggtg gtctaccctc
      181 aaggaaaccg gagaaacgcg tctgaggctg cgtgaggcgc atttctgccg tgtccggcac
      241 tgtcctgtct gccagtggag gcgctccctg atgtggcaag cccgtttcta ccagtccctg
      301 ccgcgtatcg tggcggatta ccctgatgcc cgctggatgt tcctgaccct gaccgtgcgc
      361 aactgtgcga tcggggagct gggcgagata ctgaaccgga tgaatgcagc tttccagcga
      421 ctgaaggatc gcaaggagtt ccggcctgtg cagggctgga tccgtaccac ggaagtcacc
      481 cgcagcagtg acggcagcgc acatccccac ttccacaccc tgatgatggt gccgccctca
      541 atgctatctg gtgacggtta cgtaaaacat gctcgctggg ttgaactctg gcgggagtgc
      601 ctgcgggtca gttacgaccc gaatgttgat gtccgggcag taaaaccccg gaagccgaag
      661 gacggcgaat cgctggcctg cgcgaccgcc gaactggtgc gcggtgcggt ggccgaaacc
      721 ctgaagtatt ccacgaagcc ggctgacatg gtggccgatc ctgaatggtt cctcgaactg
      781 acccgccaga cgcacaagcg ccgctttgtg gcgactggcg gcgcactcaa agacgtactg
      841 aagctcgatc aggaaaccga cgcagacatg gtgatcgggg atgatatttc cgagggcgac
      901 gatgacggtt cccgcatcgc gttcgagtgg aaaactgagg tcagaaagta ccgtcgctca
      961 ccgacgaaag ataaaaccga atcagagtaa cggggtgtcg gggctgcgcc ctgaccatga
     1021 ggactttggg catccgtcac cttggtggcg gtgtaccaaa gtcacatctt gtccgattgg
     1081 ttttactccg atgcaggata gggctaacgc cgcaaagtgc tagcactcga ttgcctagtt
     1141 cggaatccga acttagggtt ttgcgatccg taatacggat tttttttgac gggacaagat
     1201 gtgtttttgg agtaccgcca tcacgatgcg ggtaccaaaa acgatccgtc agggctgcgc
     1261 cccgacaccc tgccaacacc tcacgccgcc agattgaaat ctgccagcgt tcgccgttcc
     1321 tgaaatcgtc aaacgctaaa tcgtggggtc ccgctggctt gccagtgggt tccgatttag
     1381 tgattgacga ccgcgcccgt aaaacactgc cgacaggtgg ttgtgatcac gcagtgagcg
     1441 caaccacctg gtaagcggtt ttgggttttt ttgggggtga tttaacgcgg ttttcgggtg
     1501 gcaaatctcg cgcgcgatcc ttgtatttat acttaaggga taactctggg attcaaattg
     1561 gcctccagcc cttgtcccgc aaggcttgag ggcacatttg ctccgaaatg ggtttagaag
     1621 tattctgtcg gaagtggtcg cgttaaaagc gacccccgca acgacgggca tcgttcgggg
     1681 gcgtgaccac ctgtagagat tcgaattggt gatacggcta acacaggtga tatggcaatg
     1741 ataacacgtc agatgagcca tcgcccacaa tcgggtggtg tgc
//
