LOCUS       NZ_CP142923             4064 bp    DNA     circular CON 19-FEB-2024
DEFINITION  Escherichia coli strain B19429 plasmid pB19429_4, complete sequence.
ACCESSION   NZ_CP142923
VERSION     NZ_CP142923.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39500200
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 156.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/19/2024 10:32:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142923.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="B19429"
                     /isolation_source="Estuary"
                     /db_xref="taxon:562"
                     /plasmid="pB19429_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            join(2072..4064,1..155)
                     /gene="mobQ"
                     /locus_tag="VYI51_RS00005"
                     /old_locus_tag="VYI51_26580"
     CDS             join(2072..4064,1..155)
                     /gene="mobQ"
                     /locus_tag="VYI51_RS00005"
                     /old_locus_tag="VYI51_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_053920598.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELKTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(404..1105)
                     /locus_tag="VYI51_RS00010"
                     /old_locus_tag="VYI51_26585"
     CDS             complement(404..1105)
                     /locus_tag="VYI51_RS00010"
                     /old_locus_tag="VYI51_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1639..1893)
                     /gene="mobC"
                     /locus_tag="VYI51_RS00015"
                     /old_locus_tag="VYI51_26590"
     CDS             complement(1639..1893)
                     /gene="mobC"
                     /locus_tag="VYI51_RS00015"
                     /old_locus_tag="VYI51_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(1895..2071)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2072..2430
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(405..1105)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1986..4063
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.983"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caccgctttt tgctgccgtc aacacaccag aaaaacagcc ggatcatctg ttccgctcgc
       61 tgggttttaa tcgtacctgg gaggaatggc agcgcgagga agatgcacga acacacacca
      121 ccgagaggag acatgatcgc ggttggtcac agtgaatgtg aacaatattt tgttttgttt
      181 aacatcgtgt tatgttcgtc gatgttcatc gacagggatg aatgcaagaa gcacactcac
      241 ataacgcaag catagttttg tttttccgcc ccccaaaaat tggggggtct tttttcgctg
      301 catcagagcg tttttgacgc aatttttcac ttcaatagta ccttcgcact actaacacaa
      361 aaaaaacgca gcagggagcg ttttaggtgc cttaaaatcg attttagatc gtcatcttga
      421 tctgtttgtc ttccttgaag cgaaaatgca gggctacaac cgtgcgcccc tttttgatcg
      481 tctcaacagt tacaaccaga tcgctttttt cgttcagttc ggcgatggct ggcttgatta
      541 tcatcttatt caagtctttg aattgttgat atttatcttc aacggcaagc atggttcgga
      601 aatcatcgat agctatcgtt ctgtcaccgg tgctgcggaa ctgctggagc agctcgtaaa
      661 tgcggatgct gtaggtgctg gaaaggctgg agacgttctt cacgactacc cgcgtaaact
      721 gccccttgag ctgtgtcaga tacggcatga tggcatcgga gaaggtgata tctactttcg
      781 cctcaccttt gaaatactgc gcccggtact gcacccagcg gaactcttct cgtttttcct
      841 cgttcttgat gattactgac ctgtcccaga ggcgatctat cgcttccttg agcctagctt
      901 cagcgtgctg ccgtcccatg tcaggaaagg catcatgaaa ctctgcagcc gtgatggtca
      961 tggttttttg ttcatccggc gaaggggaat cttctccgga ttttaggcgg ctgatacaga
     1021 ccagcaggaa gcgctgttcc tgcaaagtga gcttgtagct tgcctcgata agggcgtttg
     1081 atttgtaagc tatcagatct gacatttcgc ttgctcgttg ctattggtgg cgttttttta
     1141 ctgtacgcca cttatcatat gggtggaagt tttttttttg caaccccctt tttctgtgga
     1201 ttacaccccc aaaagctcca cctttcaccc ccaaaagctc cacctttcac ccccaaaagc
     1261 tccacctttc acccccaaaa gctccactta tcttgccaga acatctttaa aatcatggtg
     1321 ttacaagacc taaaaaggtt ttaaaaaaaa agatctaaaa ataagatctt aaaaacttaa
     1381 cggcgagcct gtggataact tggggtgctg aaaacaaaaa accaataaaa acaatgcgtt
     1441 gcaataaagc agaattgcaa gtttcgaata caccccctcc aaccctgtgg aaacccagta
     1501 ttcatgcggg ttcagcgcat atcacctgcc gaaaaaccgt gcgtatgtgc tcaacctggc
     1561 gactgattca cttccgcttc ctcgcccact cgctcgctaa cgctcggtcg ctggggctgc
     1621 ggcgagcgga cgatacaatc actgttctgg cagcgcctcc agctcgttaa tcagcggcag
     1681 tatgcgcgtc acatcagcct gtcgtgtcac ctctgccttc aggaactgga gtaaccaggc
     1741 acgcaactgc acctcctccg gctttctgac ctttgccagc aatgcagcac ccacaacaac
     1801 cttctgcgct gtgtccaggc tccggctctt cgccttcagg cgctgtaatc tcgcctcagc
     1861 atcagcaatc ttctgttcga gcgttctgct catgatgtaa ctccgtgcgc gatgaaaaat
     1921 cgcattttag cgcgtcactg gtagtttaaa aactgagctg gcataatgca cggcaaatca
     1981 cgaagtgcgc acttatacaa tctccactcc gtttcgattg tgtgcgccct gcggggctga
     2041 aagaaaacta caaaaaggca ttacggcaga aatggcgatt tatcatctca gcatgaaaat
     2101 tatctccaga agcagcggct acagcgccgt agcttccgct gcctatcgtt ccggttcact
     2161 gatgcttgat gagcgtaccg gactgactca cgactacacc cgcaaaagcg gtgtggctga
     2221 ggctgtgatt ctgacccccg ccaccgcccc tgcctggtgt acgaaccgtg ccgaactctg
     2281 gaatgccgtt gagaaggccg aacgccggaa gaactcacaa cttgcgcggg aaattgaact
     2341 cgccatcccc cacgaactgc cgcaggatgc cgcacgggaa accgttctcg cctttgtcag
     2401 agaaaacttt gtcagtcagg gcatgattgc tgatgtggcg ttccatcaca tggataaaac
     2461 aaatccccat gcgcacatca tgctgaccac gagggctatc ggacctgctg ggttcagcgg
     2521 aaaggtcagg gactggaacg accggacaca tgccgagacg tggcgggcat catgggctga
     2581 ccatgcaaac agggcactgg cgaacgccgg ttatcaggaa gagatagacc atcgttcata
     2641 cgagcgacag gggcttgaga aaacgcctgg tattcacctc ggaaagtctg cctgtgcgat
     2701 ggaaacacgc ggtatcgaaa cagaacgcgg cgaacagaat cgtctgatta acaggcttaa
     2761 cctggaaata cagatttccc gcaccgaact caggaacgga gggctgacag agcctgcccg
     2821 ccgtgtggct gaccttctga atatcgggct tcctgacaac gcaacttcgt acacactacg
     2881 tgacattatc gaagcgttgc cgaaggacag taactcggca tggaaactca ccagtaatgc
     2941 catgtcgatg atggcagata tgcaggcgac gcgcagacgc tgggaagagc tgaagaccga
     3001 acgaaaggaa gcgatgagcc atgctgccag cctgaaaaaa tcatcgccgt tttcgtccgg
     3061 tttttcccgt attcccctga tgcaatgggc ggcaccggaa taccgtagtg agcagggaaa
     3121 aatccagtct ctgagccagc aaatggaaaa attacgacag cattaccgga aggtggagaa
     3181 acaggacatt cccgcgagtc agatggcatt tgaaaaacag tggcagcagt ggggggaatc
     3241 cggccttgca gctctcagag cccagttgct taaacgtgaa gaggaattgc gccggaagga
     3301 acaggaagat actgaacgta gtcgcgcgga gcagctcagg aaaaatgaca atgccgttat
     3361 ggattcaggg gtgctgagag cagtcgttac ggggtatggc gaggctccca tgcctggtaa
     3421 cggggaaatg acctgttacc ttctgcttca caaccggaac ggagaatata cgctctgggg
     3481 gaacgagctg gagaaatacc gcaccagaat ctttgagagt gttgatttaa tgcgtgacag
     3541 gagtggttat atctgtgacc gaagcgaaat cggccaacgt ccaccactgc aacgtgttta
     3601 ttcctctgca acctttgagc aactgctgac gcaggtttgc cagacatggc cgcaacatac
     3661 ccgcaatctc cgccagccga agacatggcc ggagtctttc tgtcttggag aagaccgcca
     3721 gccagccatg ccatcaccag cggcaaggaa ggtggatttt atgcagggac gtctgccacc
     3781 gacgctgatg ccggtcatga gttctgtcga ccgggaaacc cgccagcttc agcttttgct
     3841 ggtcatgggc gtggatgact ctctgggcgg tgttatccgg cttaatggta cgctgtatcc
     3901 cgcgtttgcc gttccctcag ccgataacag gcatctggtc atcagtgcgc tgaccgataa
     3961 ggggttacgg tatgcagggt atggtgttgc cgtaaatcac gatgcagaca gccacatatc
     4021 acctgcacca gaactgatgc agtttcatct gaaaacccgt gagg
//
