LOCUS       NZ_CP142914             9126 bp    DNA     linear   CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1902196 plasmid pKP1902196_4,
            complete sequence.
ACCESSION   NZ_CP142914
VERSION     NZ_CP142914.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39500185
            Assembly: GCF_036287625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 73.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 12:27:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,766
            CDSs (total)                      :: 5,643
            Genes (coding)                    :: 5,517
            CDSs (with protein)               :: 5,517
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 58 of 126
            Pseudo Genes (incomplete)         :: 75 of 126
            Pseudo Genes (internal stop)      :: 15 of 126
            Pseudo Genes (multiple problems)  :: 19 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142914.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1902196"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP1902196_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2019"
     gene            <1..291
                     /locus_tag="VYI53_RS28780"
                     /old_locus_tag="VYI53_28785"
     CDS             <1..291
                     /locus_tag="VYI53_RS28780"
                     /old_locus_tag="VYI53_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_050513510.1"
                     /translation="FDPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQ
                     QFHSQEFLNSLQGTTQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            597..860
                     /locus_tag="VYI53_RS28785"
                     /old_locus_tag="VYI53_28790"
     CDS             597..860
                     /locus_tag="VYI53_RS28785"
                     /old_locus_tag="VYI53_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            850..1149
                     /locus_tag="VYI53_RS28790"
                     /old_locus_tag="VYI53_28795"
     CDS             850..1149
                     /locus_tag="VYI53_RS28790"
                     /old_locus_tag="VYI53_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1211..1588)
                     /locus_tag="VYI53_RS28795"
                     /old_locus_tag="VYI53_28800"
     CDS             complement(1211..1588)
                     /locus_tag="VYI53_RS28795"
                     /old_locus_tag="VYI53_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            1657..1836
                     /locus_tag="VYI53_RS28800"
                     /old_locus_tag="VYI53_28805"
     CDS             1657..1836
                     /locus_tag="VYI53_RS28800"
                     /old_locus_tag="VYI53_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1864..2394)
                     /locus_tag="VYI53_RS28805"
                     /old_locus_tag="VYI53_28810"
     CDS             complement(1864..2394)
                     /locus_tag="VYI53_RS28805"
                     /old_locus_tag="VYI53_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2401..3132)
                     /locus_tag="VYI53_RS28810"
                     /old_locus_tag="VYI53_28815"
     CDS             complement(2401..3132)
                     /locus_tag="VYI53_RS28810"
                     /old_locus_tag="VYI53_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(3132..5096)
                     /locus_tag="VYI53_RS28815"
                     /old_locus_tag="VYI53_28820"
     CDS             complement(3132..5096)
                     /locus_tag="VYI53_RS28815"
                     /old_locus_tag="VYI53_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6577..6726)
                     /locus_tag="VYI53_RS28820"
                     /old_locus_tag="VYI53_28825"
     CDS             complement(6577..6726)
                     /locus_tag="VYI53_RS28820"
                     /old_locus_tag="VYI53_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6811..7068)
                     /locus_tag="VYI53_RS28825"
                     /old_locus_tag="VYI53_28830"
     CDS             complement(6811..7068)
                     /locus_tag="VYI53_RS28825"
                     /old_locus_tag="VYI53_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(7078..8763)
                     /locus_tag="VYI53_RS28830"
                     /old_locus_tag="VYI53_28835"
     CDS             complement(7078..8763)
                     /locus_tag="VYI53_RS28830"
                     /old_locus_tag="VYI53_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2078..9126
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5896..6474
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2405..3132)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tttgatccca atttttccac ccagaaagct gacacaacac gaatgatggt gccttttttc
       61 cggcagttct gggatatggg cgcaaaggat aaagcgaccg gaaaatcacg gagtgatgtg
      121 cagcaacgca ttcagcagtt tcacagtcag gaatttttaa actcactcca aggcacaaca
      181 caatttgcgg gtaccgatta ccgcagcaaa gacctcacac cgaaaaaatc aaggctactg
      241 gctgacacca tttcagctgt ttatctcgat ggctatgaag gcagacagta agtggattta
      301 ccataacgcg tcattacgcg caccgctcag acgggctcag ggcgtttccg gcggctacgg
      361 gataaaacag gcaacaaccc ccccggaaaa ctgccggaaa cgcgcccgat aaattttatc
      421 ctaatgcatg cctatgcacg caactgcatg gcatatgttt tgacgtaaaa tggatgccgt
      481 aagccctttt ttaccgactt tctgcttcat cagcctgact gacacgacca tattctttca
      541 gaccgccccc tgctaaactt ccttcatatt gccgcattaa cccgggaggc tccgcaatga
      601 gcaacactac acactacgaa aacgccaact ttcttcggga actggctgaa agcctgcccc
      661 gcatactgcc ggaaggtggc ccggacaaag ctgcgctgtt gcagcgactg gctaacgaag
      721 aactggccca ggcagagtat gaagatcagg ttcgcgccaa agtgaccgcc gcccgtgctg
      781 acacgcgtcc gggtatgacc acggaacaac tccgccagcg cctgcatggc cgttatcagg
      841 aactgcgcga tgccgtatga cgtggaatgg aagcagggag ccatcgagga tgtaacgacg
      901 ctgtttgact atatcgcgga aaattcatcc ctgtgggatg cgcagaacgt taccgaccgg
      961 gttctcgctg cggcggacaa actggccgac ttcccgcgac tctatgaagt ggactcacgc
     1021 tatggcgagg acgttcgccg gatcagtctg atgggacagc atgtcctcta tgatgttgat
     1081 gaccagacca gaaaagtccg ggttctggcc gtggtcggcc agcgccagaa tccccatatt
     1141 gttctctaat aaccggtgac ggggacgcaa ttcattgcgt ggccgtcgcc ggttggacaa
     1201 gcacagcgcg tcagcgtttt ccccacggcc accaccggcg cggtgttccg gccggggtct
     1261 ctgctgtctc cggtttgtgt tccagcagtc tcatggactg catttcctgc cgtagcccgg
     1321 ccacctcctc cctgagtgct tttatctccg cgatcaacgc gtcggaggcc attttttcag
     1381 tctcacccga cagtctcaac tcgtcccgct cgtgagtctc atcctgtctt aactcaccgt
     1441 acacccggat aagctcgctg gtatccacca cccggccgcc atcagcttcc gtacggtatg
     1501 atacgcggcc tttcgccata tcccgataca gggaacttcg ggctttaccc gtcagttccg
     1561 tcgcctgtct caccgtcacc cacgccattg tctcaccccg ctatttttga gacagtatac
     1621 ctgacaggcg cacaccatgc ccggaaggaa agaatcatga caaaacagga aaaaacagcc
     1681 ctcaatatgg ctaaatttat taaggagcag tcgctgctat tgctcgataa actgaatgag
     1741 ctggatatgg ataccccggc cgacatgtgc gagcggctac acgaggatgc ggaaacactc
     1801 tggcaggtga tgcgggataa gctcggggag gaataacagc accggcatgg ccggtgctgg
     1861 catttatttt ttgccgaacg gccaccaccg gcgggcaggt ttgtcctccg gcagttcagg
     1921 taccggcacg ttaaaatagc ggctgttata ttcccgcatc agctccgctt tcagcttctc
     1981 gttcaccgcg ctttccccgt gccgctcact caccacgtca atcatcgcgt taatctgctc
     2041 cggtgagcac ttcccggcat caaccacctg cacaccgttc tcgatggcga accgctccac
     2101 cttgcgatag aggtaatcac acagcttcat tcctgtgggt ttgttccgtt cccattgcgg
     2161 atttttacca gtgaaaaagt acgtggcttt ttcgtcgctg ttgagccgct cgtcatacat
     2221 cccgatggat ttcatcatcg ccagatgctg ggcccggtcg acaaaacaga aaacatacgc
     2281 cccggtctgc caggcgagca ccaccatatc ttccagatgc tcgctggtcg gcatgatgca
     2341 gcgctcgccg ttccggtcct ccgcgtcttc aataaatacc atgtacagcg ccataatccc
     2401 tcactccgtt gtcataaatt taaagcacgc aaacaaccgt tcacgatcca gctctgccgt
     2461 tttgcgggcc tcgccggtct gtgggttcac gatttcatcc accgacagga ttgcccgctg
     2521 caccctgacc tgcgtcgccc ggtccgggca gatgtaccag atttcatcga tgccgttagc
     2581 tttgcggttg aacagatacc cggacaccac ttcctgatac cggcgacggc tcttgacggt
     2641 gcgctccacc tcaaacgcca ccgttttacc gtccagctcc gccagggcgt ccggcacttt
     2701 catgccttta agactcagac ggtgcagaat gcgccagccg gtggcccctc tggcttccat
     2761 atcaaggcgc agggattgca cggccagccg gtgcggcaga tgtgctgccg acacccttga
     2821 cggctgaaac ggaataacat ccgccagcgg ttcgtcctcg tcgaacgaga acgccacgcc
     2881 atgcggcgtc agcccccaca cgctgacctg acggccgtcc accggatatt tggtccggac
     2941 cagcagccct ttcgccacca ggctgtcgag gaagcggtag accggctgca ccgccgcaat
     3001 ccccagccgc cgcgcaacca gctccgccgt ggaacacagc tcttcccgca accagcgcag
     3061 ggtcagccgg gttttctccg cgttccgcgc ctgtcgtttc gcgtggctga cgttgtacag
     3121 ctccagcgcc atcagtaaat ccccgcaaaa tcatcatccg ctttcggccc gccgccgtcg
     3181 gccgggggcc ggaactccgc caggggatcg gttttcgcca gcaccgggaa acgctgcaac
     3241 gggatttccc ggcgctcaac ccgcagcgga ctgctgaacg ccatccgggc cacacccagc
     3301 ccggtcatga ccgcgaaccc gtccggcagg tgttgcaggg tgttcacgtc aaacagcggc
     3361 cgcgcctcct tctggatgaa gctgtccccg ctgatatgct ccacgttccc cgcctcccgg
     3421 ctggcacggc ggcgctccac gtccaccctg atttccccgg tctgccccgc cgcccactgc
     3481 gcggactcca ggctggccgc ccggtaaaac cagcggatcg gcgtgttatc cagtaccgtg
     3541 gttttcacaa attcagcggg cagatcctgc ccgcactgcc cgaaatcgcc cagcgactgg
     3601 tgcgcgagca acagattgca gtcccggctg cgcagcgtcc ccagtgcgtt cagtacatac
     3661 ttcgacagca gatatttaat ttcatcgagc atcacgctga cgtgtggcca ctcccggaac
     3721 tcgtcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcattttttg cacacggatc
     3781 accgcctcgt cgtccatcga gccgataacg tacaggcagc cgccgccgtt gataatcccc
     3841 gccacatcga tgccactgtc cgtctgcagg acgctcaggt cagcgacgtt ttccagccta
     3901 gtgatcaacc ccttcacgtc ctttttcagg gcctccggca gcgcgtacgc cgccgccagc
     3961 acctgctgaa tgtttgcccc ctgcggaaac tgctccgcaa tcagcttcgc cgctttctgc
     4021 tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcgc ccaggttaag cccggcaatc
     4081 agcaggtttt tcagcgcgta ctggtcgaga tcgcggaaca gattcagctg cgccggtttc
     4141 cccggccgca gatccagcag ggtgaagtcc ggacagtgcg cccggaagac gtgcggcgcc
     4201 catgcgtccc ctttggggtc gaagtacacc accgcgtccc cgaaatcgcg tacgcagcga
     4261 atgagcgcgt tggtggccat cacgctcttg ccgctcccgg gcaggccgag gatctgaatg
     4321 ttggtcttgc gccaggtatc acgcggaacc agcaccggct tgccctgctc atccttaccc
     4381 agaaacaccg cctgctcctg ccgggcctgc tccgaggaaa aatcggccag cgcattaacc
     4441 ggtaccggct ccccgaaatg caggtggcgc acgtcggtca tctctcctgt ccgctggctg
     4501 ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcagc cgcaaaatga
     4561 ccgtcaagca gcaggaacgc cagcagccgc gccaacacaa tcccgacagc cagcccgccc
     4621 gcgcaccagg caatcccgaa caggttagcc ggctgcagca ggtaataatc tgcgtttgcg
     4681 tagaggtccg gacgcaggac ggtttctccc cagctctcga taaacacggc gacaaccagc
     4741 ccccccagaa gggccagacc ggccgcgccg gtcagccagt acgcgtagtt ccagacatgt
     4801 cgacgtgccc tgaccatccg cccgggcgaa tccagcgagg ccagcaccga aaatggcgct
     4861 ggccgtttat tatcgcgctt atagcgatcg gcatactccc gtcgctcctt atgactcagc
     4921 cccacaaaac gcatccgtcc ggcccaaagt tcagccagac cgaaaaacag gccgataact
     4981 aggaaagccc cggccagacc gtaatactcc tggtacagcc agctgaccca cggataaatc
     5041 tgccgggcca gctccggaat gtgccacttc caggcaagcg aatccgtatt aaacatgttc
     5101 tctctccttc agacgatggt ttgccggcgt gatgtgcagc ccccgctgca caagccgcgg
     5161 acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc agctttcacc
     5221 acggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag gaacaacgag
     5281 cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc cggtgttccg
     5341 gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag gagcagcttt
     5401 gctgcttcgc caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg cgtgagcctg
     5461 acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacggagc cagaaagagg
     5521 gggagagacc gtgcgtcggt ggcgtccagc cagcgtaagc aggggatatc cccctgaggt
     5581 cagccacgcc ctcgcgggga tgacctgtat ttctccgtaa ggagaaaaaa gcaacagaga
     5641 taggttaacg taaaatgttc agcgaaaaac cgatatgatt cacctatcct ggtaatttat
     5701 tatcagggaa tataaagact taatcagggg acaatatgcc acatcagcgc gtccggtgtc
     5761 cgtcttgtag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta cgctccggcc
     5821 gtgagactgc ggcgggcgct gcggacacat gcaaagttac ccacagattc cgtgaataag
     5881 caggggacta acatgtgagg caaaacggca aggccacgca ggtggcgttt ttccataggc
     5941 tccgccctcc tgactgagtt cacataaaca gacgcttttt cggtgcatct gtgggagccg
     6001 gtggctcaac catgaatcag tcaggagtgg cgaaacccga caggacttaa agatcatcac
     6061 cgtttccgat gaatcgctcc ctcttgcgct ctcctgttcc aaccctgccg tttcccggat
     6121 acctgttccg cctttctccc ttgcgggaag tgtggcgctt tctcatagct cacactctgg
     6181 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga accccccgtt
     6241 cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc ggaaaagcac
     6301 gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct
     6361 aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt
     6421 ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac
     6481 cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca
     6541 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     6601 ttcagaggag gacgatggtg ccaccgttcc accctgaaca tcccggatat agtttgcctg
     6661 acatgcgacc agaagaaagc ctgaaaaaat caatataaaa agaaaaacaa cctttacctt
     6721 tttcatatac cttccctgta tttaaaaacc ccggtattca tccctgaaca ccggaagtct
     6781 gtatttcaaa tcatccttga taacttttgc ttaccagtta ccatcccggt aaacaaagga
     6841 tacaaagtaa tcaaatttac taatatcgat tacattttta aagtgtggct gtaatatcga
     6901 aacccatgat ttttcaacat caaaccaacc attattaata ttatccttta aaggcatccc
     6961 cagactttca atgacagaac catcatcacc gaagtctttt gagtattcac cacctttaaa
     7021 ctcttcggtt ttcttatcaa accaatgaat atgtaattta agccccatat ttacctctta
     7081 aagatatttt ttaatgtttc gttttggatc cggcccttta acctgcttac ccgtttttgg
     7141 atcgaatgcc ccgaggtgtt cgccatcact ggcgcggtac ccttcaagct caccgtgctg
     7201 ggaatcccat tcataaattt tacgcttttt atcaccatac catcgggccc gcttaccacc
     7261 accaccttgt ttaggggttt taggtttacc ctctttcaac tctcccaacc ccttaatatc
     7321 ttcagtcttg ggatcaggaa aataatcatg gccgtaatct ttagttccct tacgaggttt
     7381 tttcttttcc tcatttaatt tattttctgc atccttcgct tttttctcct tttgtttccg
     7441 gctctccatc gccccggaaa gcgcagcctt atccctggat aaggattcat tcatttcgtt
     7501 taaagatgtc tgggcagctt ttaatttctc attctgattc tgaatatcct tatcaagata
     7561 gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag tcacaaaatc
     7621 atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag cagcccttgc
     7681 accggggatc gccttctctg tcgatgcaat gcgactgttt aaggtcgcaa tatttttatc
     7741 ttcagcatcc agctcagctt tcgctttatc atactctctt ttcagcccct cttccgggtg
     7801 agcagcgtcc catgcctgct ggcgacgctt ttcttcctcc agacgctgtt ttacctgtgc
     7861 cggagtaaga acatccgtca ccgacacata gaccggtttc tgtccgctct ctttcgggaa
     7921 acgaataacg gcttcacggg agttaccacc ggccgtaaaa ccagccggac gtgaatcacc
     7981 tttttcagca ataatgcctt ttggcggggc tttcgcggcc ggaacacctt taggtacgct
     8041 cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac gttttgtcgg
     8101 tttcgcatcc accacaggaa cactcattgg ccggccggtg acaaccgcaa tatgctgccg
     8161 ctcgtccttc acaatatccg ccacacgttt agtgacgctg accgtcgcct ggtccagcgg
     8221 cagggagctg accggcgtct ccgttaccgt gtctgccggc aatgacgtca cgatttttga
     8281 catcattttc ggatcatctt ttgctatctc agaaggcagc actccgtaaa tcgcaatccc
     8341 ccacagacca aacttaaacg gacctttcag ggcagccagc acatcagcaa cagcggccga
     8401 caacgcatcg ccggaaacgg ataaagccag tccttcagcc cccggagtgg ccaaggcagg
     8461 cagaccgaag gccatcgcgg tggccgaaga ctgtccgcca ccagagctcc cgctatgatt
     8521 gccgctgtta ccgccattac caccgggttt actcccgccg ttaccaaact cccccgggtt
     8581 atagttatga atatccccgt taggtgtatg cgtcgtaccc cagcccggcc cgctgtttgg
     8641 atccgtacca ccacctgatg aagggccacc tttacctgat gtaccgttca cattcccact
     8701 ggcctgacca ccattgtgtc ccagacctga attacccgga cctcgtccgt ctccgccact
     8761 catatacttt tcctctttga cttttaaaac aataaactaa aataaaatac tgtacatata
     8821 accactggtt ttatatacag tataaaaatt acgctgcctt gtttttcatg tcaagtctgt
     8881 aaatttgcgt ttttgtgaaa atgatcaaaa aaacagccac tcacaccatg cgcaatcccc
     8941 tccagatgca acaaaaacca gcacaaacgg cgccgttctc cggcctcagt atgttttact
     9001 gaaatcatta tatgagatac cagaaagccc cctccgggac aggaaaagaa acgggggtct
     9061 ggttcgggtt gttcggtcac tgaacacggt cagaaactga tgataagata gcctgcggat
     9121 atataa
//
