LOCUS       NZ_CP142903             3074 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B18188 plasmid pB18188_4, complete
            sequence.
ACCESSION   NZ_CP142903
VERSION     NZ_CP142903.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39499813
            Assembly: GCF_036287585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Maguire,M.
  TITLE     Spatiotemporal and genomic analysis of carbapenem resistance
            elements in Enterobacterales from hospital inpatients and natural
            water ecosystems of an Irish city
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Maguire,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Bacteriology, University of Galway,
            University Road, Galway, Galway 000000, Ireland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 173.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 12:18:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,812
            CDSs (total)                      :: 5,687
            Genes (coding)                    :: 5,564
            CDSs (with protein)               :: 5,564
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 49 of 123
            Pseudo Genes (incomplete)         :: 79 of 123
            Pseudo Genes (internal stop)      :: 14 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142903.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18188"
                     /isolation_source="Hospital sewage"
                     /db_xref="taxon:573"
                     /plasmid="pB18188_4"
                     /geo_loc_name="Ireland"
                     /collection_date="2018"
     gene            complement(222..1580)
                     /locus_tag="VYI55_RS29050"
                     /old_locus_tag="VYI55_29055"
     CDS             complement(222..1580)
                     /locus_tag="VYI55_RS29050"
                     /old_locus_tag="VYI55_29055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_329518097.1"
                     /translation="MPNNKNLNERSRKFQFIHLEAYSLQANKQGGKNAKFNKEVKARTV
                     SEVLGEVLRKDGFCNHIQEPEAPGVLYGDLNNIETDCKTYHQNHKNIDKNGKAKALRSD
                     ANVLIAGVVSLEGIKENYDDWDKYKTDVVGYLKEKYGDNLAAVVEHTDEENPHLHFYIV
                     PRSGQRLDELHDGKNAVLELKKKEPKALKGKQNKAYIEGMRNFQDDFYNKVSKNFGLTK
                     IGPARDRVSRKIYFEQKKAAEEYRKTLKQIEQNKFFIDSEFEQAKKNIENEIKKEKNNF
                     EKEKRKIVKKVVKETNEEAMKNYENKNYINKMIFSMTYNKTKIFNQEKQIEHLIKEGKK
                     HFNRKNEYKKEILRLKDFEDKYTNLRKDFGKRLNNALGEVKVENEQLKRENNLLISENE
                     NLKAENAMAKYAVEFLSKLKNKMGSKFESFKNDLFPKSSHKHTTENPKVKRRF"
                     /name="plasmid recombination protein"
     gene            complement(1570..1767)
                     /locus_tag="VYI55_RS29055"
                     /old_locus_tag="VYI55_29060"
     CDS             complement(1570..1767)
                     /locus_tag="VYI55_RS29055"
                     /old_locus_tag="VYI55_29060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_329518095.1"
                     /translation="MLKTDLNIVKIDYVNFESQKHLFLYKEQIEYFFNDYLKNIDGNIN
                     LFIEENIIKEFFDWEDDYAE"
                     /name="hypothetical protein"
     gene            complement(2069..2659)
                     /locus_tag="VYI55_RS29060"
                     /old_locus_tag="VYI55_29065"
     CDS             complement(2069..2659)
                     /locus_tag="VYI55_RS29060"
                     /old_locus_tag="VYI55_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_329518096.1"
                     /translation="MKTKAYINSDQADFLKSQTEVEDLNELNIHATAKKKTSFNSPYSI
                     VFHDSITAISQMGLKPNAYKIVIYLFSKISMGNIIINFSQKKIAQDLGLQPSNVSRAFG
                     QLFEKQILIKDDVEGHTYLNSNLATMGIPKNFNQKTMENLKKSQVETEDFKNNINLFKS
                     TNSKKINKTKPNSKKENIKNDDDVDLSNVEPLF"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ggggacaaag gcaaaacatc atcgacaaaa agcatccagc cgaccagcta ccgccccggc
       61 cgttcggcct gattgtgttt agtgtcttaa acgtaaaacg ccgtatagag cgttacagag
      121 agcctgtgac cccttcctgc aaagcctgtt ttgacctttc gccccatagg ggctggggcg
      181 tagcgtgaag agcgtaagcg atgaaccaat aagaacgaag ttcagaatct tcttttgact
      241 tttgggtttt ctgttgtgtg tttatgcgaa gacttaggaa atagatcgtt tttaaaactt
      301 tcaaattttg atcccatttt attttttaac ttgcttaaaa attcaacggc atatttagcc
      361 atagcattct cagcctttaa attttcattt tctgagatca ataaattatt ttctcttttt
      421 aattgctcat tttcaacctt aacctctccc aaggcattat ttaacctttt cccaaaatct
      481 ttccttaaat ttgtatattt atcctcaaaa tcttttagcc ttaaaatttc ttttttatat
      541 tcatttttcc tattaaaatg cttcttccct tctttaatta aatgctcaat ttgtttttcc
      601 tgattaaaaa ttttagtttt gttgtaagtc atcgaaaaaa tcattttatt tatatagttt
      661 ttattttcat aatttttcat tgcttcctca tttgtttctt taactacttt tttaacaatt
      721 ttccttttct ctttttcaaa attatttttt tcttttttta tttcattctc aatgtttttt
      781 ttagcttgtt caaattcgga atcaataaaa aatttatttt gctctatttg ttttaaggtt
      841 ttcctgtatt cttccgctgc ttttttttgc tcaaaataaa tttttctact cacgcggtcg
      901 cgtgctgggc caattttagt aagtccgaaa tttttggaga ctttattata aaaatcatcc
      961 tgaaaatttc gcatcccttc aatatatgct ttattctgct tgcccttcag ggcttttggt
     1021 tctttctttt ttaattctaa aaccgcattt tttccatcgt gtaactcatc gagacgctgc
     1081 cccgaacggg gcacaatgta aaagtgtaga tgtgggtttt cttcgtctgt atgctctacc
     1141 acagccgcta aattatcccc atatttttct tttaaatacc ctacaacatc agttttatat
     1201 ttatcccaat catcataatt ttctttaatc ccttccagac taacaacacc agcgatcaga
     1261 acatttgcat cgctacgcaa tgcttttgcc tttccgtttt tgtcgatgtt cttatggttc
     1321 tggtggtaag tcttacaatc ggtctcaata ttatttaaat cgccatataa cacaccagga
     1381 gcctccggct cttggatgtg attacaaaac ccatcttttc tcaaaacctc acccaaaacc
     1441 tcggacactg tacgagcttt tacctctttg ttaaatttag cgtttttccc gccctgcttg
     1501 ttagcttgga ggctgtaagc ctccaggtga ataaattgaa attttctaga tcgttcattt
     1561 aaatttttat tattcggcat aatcatcctc ccaatcaaaa aattctttaa ttatattttc
     1621 ttcaataaat aaattaatat tcccatctat attttttaaa tagtcattaa aaaaatattc
     1681 tatttgctct ttgtataaaa aaagatgctt ttggctttca aaattcacat agtcaatttt
     1741 aacaatattt aaatcagttt ttaacatata accccctata tagtttttat ttatataatt
     1801 taaataatat atttcaaaaa aaagtcaatt tttctggggt gggtttgcga ttaaattttg
     1861 ctttaattta taagcatagg aaccgactat gcttataccc taaagggtat aagcgcggcc
     1921 ccataaatgg ggaaaacaaa aaggacaaca ccttaaaaaa actcataaaa aaaaagccct
     1981 tccgggcttg ttttttcttc gtctttttaa atcggcaatc ctatattttc caaattttat
     2041 atttggggaa tataggattt aaaagacttt aaaataatgg ttcaacattg cttaaatcaa
     2101 catcatcatc atttttaata ttttcttttt tgctatttgg tttagtctta tttattttct
     2161 tagagtttgt agatttaaat aaattgatgt tatttttaaa gtcttcagtt tcaacttgtg
     2221 atttttttag gttttccatt gtcttttgat tgaagttttt aggaatcccc attgttgcta
     2281 aattactatt taaatacgtg tgaccttcta catcatcttt tattaaaatt tgtttttcaa
     2341 acaattgacc aaaagcccta gaaacgttcg acggctgcaa gcctaaatct tgcgctattt
     2401 tcttctgcga gaaattaata ataatatttc ccattgaaat ttttgaaaat aaataaataa
     2461 ctattttata agcgttaggt tttaaaccca tctgacttat agctgttatt gaatcatgaa
     2521 aaacgattga ataaggacta ttaaagctag tttttttctt cgctgtcgcg tgaatattta
     2581 attcgtttaa gtcttcgact tctgtttgag attttaagaa atcagcttga tcgctgttta
     2641 tgtaagcctt tgttttcata ttttattctc ctaatttttt actttataca acctatataa
     2701 gcgattaaaa gataatgtca atatcaaaac gataaaaaaa atatcatttt aacaagaaaa
     2761 cttatcaata gagggttttt tgaacgatta tgattttcat aacttcgatt atgaatttca
     2821 taatttggga ttatgatttt cataactgga aattatgatt ttcataactg aaattctatt
     2881 aaagtgttga tatataaggg tttatgtagt gcttaagaag ttaagaaaat aagaaagaag
     2941 aaacgggaaa aagacaactt cggctcgccg cccggccaac ccctcacagc cccacgggca
     3001 agccgtggaa ctgctcgcgg ttatgtaaac gtcacatcaa aaccaaatcc tgaaggattt
     3061 tatctggtcc ccaa
//
