LOCUS       NZ_CP142896             7223 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Acinetobacter baumannii strain B20AB10 plasmid pB20AB10a, complete
            sequence.
ACCESSION   NZ_CP142896
VERSION     NZ_CP142896.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39326236
            Assembly: GCF_036287275.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Moon,D.
  TITLE     Genome sequence of Acinetobacter baumannii in Korea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Moon,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2024) Division of Antimicrobial Resistance
            Research, National Institute of Health, 187 Osongsaengmyeong2-ro,
            Cheongju-si, 28159, Korea, Cheongju-si, Chungcheongbuk-do 28159,
            South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.9.3; pilon v. v1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 571.239x
            Sequencing Technology             :: Illumina MiSeq; Minion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 12:08:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,982
            CDSs (total)                      :: 3,886
            Genes (coding)                    :: 3,805
            CDSs (with protein)               :: 3,805
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 27 of 81
            Pseudo Genes (incomplete)         :: 50 of 81
            Pseudo Genes (internal stop)      :: 10 of 81
            Pseudo Genes (multiple problems)  :: 6 of 81
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142896.1.
            Bacteria and source DNA available from National Institute of
            Health.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="B20AB10"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pB20AB10a"
                     /geo_loc_name="South Korea"
                     /collection_date="2020-07-02"
     gene            join(<7223..<7223,1..1439)
                     /locus_tag="VP466_RS19275"
                     /old_locus_tag="VP466_19305"
                     /pseudo=""
     CDS             join(<7223..<7223,1..1439)
                     /locus_tag="VP466_RS19275"
                     /old_locus_tag="VP466_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890075.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /name="DNA helicase"
     gene            1443..1730
                     /locus_tag="VP466_RS19280"
                     /old_locus_tag="VP466_19310"
     CDS             1443..1730
                     /locus_tag="VP466_RS19280"
                     /old_locus_tag="VP466_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002075481.1"
                     /translation="MEDFSWLQDMIADDDLGLLNVKKKMSALTADEQLVSKFNEINNFV
                     TQHGRVPTKNMANITEYMLASRLEAVQANPEQYMALADYDEHNLLPQHQD"
                     /name="hypothetical protein"
     gene            1735..2649
                     /locus_tag="VP466_RS19285"
                     /old_locus_tag="VP466_19315"
     CDS             1735..2649
                     /locus_tag="VP466_RS19285"
                     /old_locus_tag="VP466_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752788.1"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WP_002075484.1"
                     /translation="MSDIKSLEDIFNDDDLGLLDDGKDGDIFKIKHVPVQRAAADEVAK
                     RKRCKDFDQFEHLFKDVHRELREGKRKLLPFHDKSYQLEKGSYYVLNGLLVYLADFTFT
                     REDKTVNKVRTRKDGRTRTIFENGTESTMLYRSLAKALYNDGRIVSETNEQANSNFYTN
                     FGGITENDTSTGYIYILKSHSKEPKIQSLEHLYKIGFSTTTVEKRIANAKKEPTYLMAD
                     VEVIAEYQAFNINPQKFEYYLHAFFGEACLDLLVADKGGKNHQPKEWFIAPLNVIQQAV
                     ELLVNSQIMNYKYNKLTKEIELK"
                     /name="GIY-YIG nuclease family protein"
     gene            2823..3440
                     /locus_tag="VP466_RS19290"
                     /old_locus_tag="VP466_19320"
     CDS             2823..3440
                     /locus_tag="VP466_RS19290"
                     /old_locus_tag="VP466_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000807825.1"
                     /translation="MAEIITIASRKGGVGKTTIAINMVVALSNKGKTLLVDADEQQSAM
                     KWNKHRKEKIDAISIHKDLIAELEKLDGEYQYILIDVAGRDSEIFREALLISDKLIVPT
                     QASLLDLDVIPYLNEKVKTAKKTNPNLVSMVVINRASTNAKSNETEQAREFVADYEEFK
                     LLQTTLHERKQYRDAVLESKSVLEMAASKAKDELNQFLIEVL"
                     /name="AAA family ATPase"
     gene            3440..3835
                     /locus_tag="VP466_RS19295"
                     /old_locus_tag="VP466_19325"
     CDS             3440..3835
                     /locus_tag="VP466_RS19295"
                     /old_locus_tag="VP466_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000147423.1"
                     /translation="MTGNNTSNKFKAARENKDFVQDVNSDAADPMPKQITVTSRNKSQI
                     TLEQNTLSQRERSRVKNGRNLTIPLFAEELLIIETAVESLSQKEDVSINSFIRSIVLDK
                     CKKVIGKDAFDKLNENKLNVVKAKKEK"
                     /name="hypothetical protein"
     gene            3985..>4398
                     /locus_tag="VP466_RS19300"
                     /old_locus_tag="VP466_19330"
                     /pseudo=""
     CDS             3985..>4398
                     /locus_tag="VP466_RS19300"
                     /old_locus_tag="VP466_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006647583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /name="DNA helicase"
     gene            <4398..5057
                     /locus_tag="VP466_RS19305"
                     /old_locus_tag="VP466_19335"
                     /pseudo=""
     CDS             <4398..5057
                     /locus_tag="VP466_RS19305"
                     /old_locus_tag="VP466_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005025424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            complement(5162..5918)
                     /gene="blaOXA"
                     /locus_tag="VP466_RS19310"
                     /old_locus_tag="VP466_19340"
                     /pseudo=""
     CDS             complement(5162..5918)
                     /gene="blaOXA"
                     /locus_tag="VP466_RS19310"
                     /old_locus_tag="VP466_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032007272.1"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /name="blaOXA"
     gene            6098..7204
                     /locus_tag="VP466_RS19315"
                     /old_locus_tag="VP466_19345"
                     /pseudo=""
     CDS             6098..7204
                     /locus_tag="VP466_RS19315"
                     /old_locus_tag="VP466_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612069.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
ORIGIN
        1 acaatattct aggtactgtt cttaaatctt taatggacaa taccacggct catattgttg
       61 cgatgacagg ttcatacttc cgtggcgata gcgttcctgt acttacgcct gaagatgaag
      121 cgaagtttga taaagtttct tttaactatt acgaacaact caatgggtat agctatttaa
      181 agtcattagg cattggttat cacttctata aaggccgcta ctttgcagaa gatgatgaag
      241 gggttatcgc aattgctgaa gttttagata ccgataaaaa aaccataatt cacattccaa
      301 atgtaaattc aggtgaatca accaaagata aatatgatga agtggacgcg atcctcgaaa
      361 tcattggtga atttgtcgct aaagatcctc atactggaat tatcaccgtc aagcgtgcag
      421 atggtaagat cttaaaagta gcagacctcg ttgaagaaga tggtcgtgat aaagttgttg
      481 aatatctacg taacattaaa accgtagatg atctttatct catcattgcc ctaggcatgg
      541 cgaaagaagg ctttgactgg caattctgtg aacatgcttt aacgattggt tatcgtggct
      601 ctttgactga aattgtgcag attgtgggac gttgtacacg agatagctca aacaaatcac
      661 atgcgcagtt taccaatctt attgctcaac ctgatgcaca ggatactgaa gttaaatttg
      721 cagtaaacaa catgctgaaa gcaattactg catctttgtt aatggaacaa atacttgcac
      781 caaacttcaa gtttaaaacc aaacgtgatg atgaagatat tgttccgcca ggaacactaa
      841 cgattaaagg ttttaaagaa ccaagcacgg caaaaacaaa ggcgattatt gaaaatgatc
      901 ttgtggatct aaaagcagtg attcttcaag atgctaaagt gattgcaggt tcagcaggta
      961 aggttgagcc tgaagtggtg aataaaatcc ttattcccaa gattatccaa acaaaatatc
     1021 ctgacttaac gaaagcagaa cttgaagaag tgagtcagta tttagtcgtg gactcagtga
     1081 ttagcacagc agaagtcaaa gaagttggcg ataaaaagtt tattcgaatg gcagataaat
     1141 ttatcaatat cgaagacctg aatattgatt taattcaatc tgtgaatcct ttccaaaaag
     1201 cgtttgaaat cctatcaaaa gaagtcacag tcaatgtgct caaattgatt aaggaagcaa
     1261 ttgaagcaac taagattagt atgactcaag aagaagccat tgagctatgg cctaagttaa
     1321 aagccttcaa agttcagttt ggtcgtgagc ctagtctaga gtcaaataac ttaattgaaa
     1381 aacgattagc cgaatgccta atctatatcc gtgagcaacg tagacgagct ggagtgtaaa
     1441 acgtggaaga ttttagctgg cttcaagata tgattgctga tgatgactta ggtcttttaa
     1501 acgtaaagaa aaagatgtct gctctaacgg ctgatgagca attagtctct aagtttaatg
     1561 aaattaataa ttttgtgaca cagcatgggc gtgtgcctac gaagaatatg gcaaatatca
     1621 ctgaatatat gctcgctagc cgtctagaag cagttcaagc caatccagag caatacatgg
     1681 ctttagcaga ttatgatgaa cataatttgc tgccacaaca tcaggattaa agatatgtca
     1741 gatattaaat cacttgaaga tatttttaat gatgatgatc taggtttgct cgatgatggt
     1801 aaagatggtg acatctttaa aattaagcat gtgcctgtcc aacgtgcagc agcagatgaa
     1861 gttgctaaac gtaaacgctg taaggacttt gatcaatttg agcatctatt taaagatgtt
     1921 cacagagagc ttcgagaagg taagcgtaag ctattaccat ttcatgataa aagttatcag
     1981 ctcgaaaaag gcagttacta tgttttaaat ggtcttttgg tttatttggc agattttaca
     2041 tttactagag aagataagac cgttaataaa gtcagaacca gaaaagatgg tagaactaga
     2101 actatttttg aaaatggtac tgaatccaca atgctctatc gatcactagc taaagcatta
     2161 tataacgatg ggcgaattgt ctctgaaact aatgagcaag caaattccaa tttctatact
     2221 aattttggtg ggattacaga aaatgataca tctactggtt acatctacat cttgaaatct
     2281 catagtaaag aaccaaaaat tcagtcactt gaacatctct ataaaattgg attttcaacc
     2341 acaactgttg aaaaacgtat tgctaatgca aaaaaagaac cgacttattt gatggctgac
     2401 gttgaagtaa ttgctgaata tcaagcattc aatattaatc cacagaagtt tgaatattac
     2461 ttacatgctt tctttggtga ggcttgcctt gatttattgg tcgcagacaa aggtggtaaa
     2521 aatcatcaac ctaaagaatg gtttattgcg cctttaaatg tgattcaaca agccgttgaa
     2581 ttattagtaa atagccaaat tatgaattac aaatacaata agctcacaaa agagatagaa
     2641 ttaaaatagt ttcataataa acctatgata gaactatgat agaactatga tagagctata
     2701 atgggtttat aaaacaacta aaaaacattt tttttaattt tattaatgtt ctataatgca
     2761 acttaaataa cctattggta tattccttct atccctagaa atatctgaaa gagtgagaat
     2821 aaatggctga aataattact attgctagta gaaagggtgg ggttggaaaa accactattg
     2881 caataaatat ggttgttgct ttaagcaata aaggtaaaac tttattggta gatgctgatg
     2941 aacaacaatc tgctatgaaa tggaataagc atcggaaaga aaagatagat gctatatcca
     3001 tacataagga tctaattgct gagttggaaa aactagatgg ggagtatcag tacattctca
     3061 tagatgttgc tggccgtgat tctgaaatat ttagagaagc acttcttatc agtgacaagc
     3121 taatcgtacc cactcaagca agtcttcttg atttagatgt aatcccttat ctaaatgaaa
     3181 aggttaaaac agctaaaaaa accaatccaa acttggtttc aatggttgtg attaatagag
     3241 catcaaccaa tgccaaatcc aatgaaacag agcaggcaag agaatttgtt gctgattatg
     3301 aggaattcaa gttattgcaa accacattgc atgaacgaaa acagtataga gatgcagttc
     3361 ttgaatcaaa gagtgttctt gagatggcgg catctaaagc taaagacgaa ttaaatcaat
     3421 ttctaattga ggtgctgtga tgactggaaa taatacaagc aacaagttta aagctgctag
     3481 agagaacaag gattttgtgc aagacgttaa ttcagatgca gcagatccaa tgcctaaaca
     3541 aattactgtt actagcagaa ataaaagtca aataaccctt gagcaaaata ccctaagtca
     3601 acgagagaga tccagagtta aaaatggtag gaacttaacg attccactat ttgcagaaga
     3661 acttttgatt atagaaactg ctgttgaaag cctttctcaa aaagaagatg tttctatcaa
     3721 tagcttcatt agaagtatcg ttcttgataa gtgtaagaag gtgataggaa aagatgcctt
     3781 cgataaatta aatgaaaaca agctaaatgt tgttaaagca aaaaaagaaa agtaggaaaa
     3841 ggaaacatta gccaggataa gatagtgttt gattatcaca ctatctttcc tactctacgc
     3901 aaaatacttt tttttaataa acattggaaa acatattaac ttattgaaaa taaatgtatt
     3961 aatatagaaa aaaattgaca tattatgcat catcacacga acatggggat gcgagctatg
     4021 caagctcgcg ccttcgaatc tcgtaatagt cagtatttgc tcttaaaagc ccctcctgca
     4081 tcaggtaaat ctcgtgcatt aatgtttttg ggtttagaca agatacacaa ccaagggtta
     4141 agcaaagtta ttgtagctgt acctgaacgc tcaattggcg gttcatttcc tggcacaaaa
     4201 ctaagcgaaa gtggcttttt tgcagattgg gatatcaaac ctgaaaacaa cttatgtact
     4261 gctggtggcg ataaaagcaa agtaaaagcc tttaaacact ttatggaagg tacagatcaa
     4321 attttagtat gtacacatgc cacactaaga tttgcttttg aagagcttga agatcatgta
     4381 tttaataata cattagtcaa aatacacgac aatttttgca gaatctgcca gccaatgatg
     4441 ttctccttac tggttcacgc ggcacaggaa aatcttcaat tgtccgtgcc cttttaaccg
     4501 agtataaaga tcaaggatta cgtttaattg aaattgaacg tgatgatctg tccgatcttc
     4561 ctgaaatcca gaagctcatc cagaagcgtc cggaaaagtt cattgtctac tgcgatgatc
     4621 tggcctttaa tgctgaagat gaaaactatg gccgcttaaa aagtgtactt gatggctcat
     4681 tacagtcagg ttcaagtaat tttattattt atgcaaccag caaccgtcga catctgttac
     4741 cagaatttat gcatgagaac acgccagtga ctcgtgtaga tgtaccgcaa tataccgagt
     4801 tgcatccaca agaagccatt gaggaaaaaa tttcactctc ggatcgtttt ggaatatggc
     4861 tttcattcta tccaatggat caaaacctgt atttaaccat tgtagaacac tatcttgcta
     4921 aaacagatat gccaatgaat gacgaagccc atcttgaagc cctacgctgg tgtcaggcac
     4981 gtggtcaacg gtcaggacgt gccgcctatc agttctctaa acactggatt ggatcacaac
     5041 aactaaaagc actgtaaaat tttaaactag aaaaattaac aggtttcatt attagtcaga
     5101 ttataaaagg cccatttatc tcaaatgggc tttttataaa attagagttt ctgtcaagct
     5161 cttaaataat attcagctgt tttaatgatt tcatcaataa ttcattacgt atagatgccg
     5221 gcatttctga ccgcatttcc atatttaatg caaaagcgac aatttttcca tctggctgct
     5281 caacccagcc ttttcaacca gcccacttgt ggttttatat ccattgccca accagtcttt
     5341 ccaaaaattt tgtagccatt actctcttct aaaagaagca tattttttac atttagcctg
     5401 cactttttca ctaaatggaa gctgtgtatg tgctaattgg gaaacaaact ctacctcttg
     5461 aataggcgta acctttaatg gtcctaccaa ccagaaatta tcaacctgct gtccaatttc
     5521 agcattaccg aaaccaatac gttttacttc tttttgcatg aggatcaaga ccgatacgtc
     5581 gcgcaagttc ctgatagact gggactgcag aaagcttcat ggcttctcca agtgtcatgt
     5641 ctttttccca agcggtaaat gaccttttct cgcccttcca tttaaatatt tcattaatat
     5701 ccgttttctg gttctccaat ccgatcaggg cattcaacat tttaaatgta gaggcttgca
     5761 catcttctgt atttgcgcgg cttttccgag cgttaccatt tcggctaatt tttttatctg
     5821 tttgaatcac cagcacacct gaggtgtttt tttcatcaaa gtattgatga atcacctgat
     5881 tatgtccctt gaacaatctg actccgggtt tcatttattc aattatgctg aaccgtacaa
     5941 ccagaaagaa aaagagaagc aaccacatag caagtaaaat atttattcat tttaaacacc
     6001 agatcaatcg aagtaataaa aaagagctct gtacacgaca aaaatagata actcgttgaa
     6061 ataatgtcat aataattgtt ttctaacgac gaatactatg acacatctca atgagttata
     6121 tcttatctta aacaaatctc taaaatggaa caagtcacat ttaaagtgct ttgcgctagt
     6181 aatgcttgtg aatttatctt taaagcagac attgtaatct ttcttctgca tctaaagcct
     6241 tgcccatcaa gtgcttacca caatcatttt atggacgtat gcagcgcttc tttttgcagg
     6301 tcagtatttt gattatcgtc aaatttctca gttgattttc aatacttttt caatcaacca
     6361 agtgcaactg actttagata gaaccaattg gaaatgagga aaacgagatg tttgatatac
     6421 cctgatgctt tcaataattt atgcgtggaa tagcgatacc tatcctttaa ggataattgc
     6481 ttcgaataaa cgtggaaatt cagattcgaa agagcgcatt gctttaattc aacgttttat
     6541 gccgtttttg gtaccgaccg tattgtgaat gtttgtttcg cagacgagag agtttatcgg
     6601 tgagcagtgg tttacatggt taattgaaca aagacatcaa cttctgcatt cgtgttaaaa
     6661 aaacttccat tctcaccaat catttaggaa agaatcataa aattagtgat ttatttcgcc
     6721 atcttaaagt tggtcaaatt gaatgtcgta aacgacggat tttggttggt cgggtgaaac
     6781 tatatataag tgcactacag ttagaaaatg gagagctttt actcgtcgtt tctcctcagt
     6841 ttaatgccaa tgctattcag gtatgcatgc gctgggaaat tgaaacctta ttcagttgtc
     6901 tcaaaggacg cgggtttaat cttgaaaata cgcgcttgac agaccctaga cgggtgaaaa
     6961 aattgattgc ggtgttagct ataagcttct gttggtgtta cttaacgggt gaatggcaac
     7021 atgatcaaaa aaaagcgata aaaataaaga agcatggacg actctcaatg agtttatttc
     7081 gctatccatt cattatgaaa aaatggcaat tcagcgttta attggttttg ggaaaaaaga
     7141 agagtttaag gaaattttgg caattttaag aaggcagaat cctgatagga taagggttct
     7201 gtgaaatttg tgccgtgtac aga
//
