LOCUS       NZ_CP142858            11952 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Staphylococcus hominis strain Sh8213 plasmid pSh8213-3, complete
            sequence.
ACCESSION   NZ_CP142858
VERSION     NZ_CP142858.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39497361
            Assembly: GCF_036287195.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus hominis
  ORGANISM  Staphylococcus hominis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Tang,S., Chen,L., Wu,H., Li,X. and Yu,Z.
  TITLE     Staphylococcus hominis strain Sh8213
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Tang,S., Chen,L., Wu,H., Li,X. and Yu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2024) Department of Otolaryngology, Zhuhai
            People's Hospital, Kangning Road No. 79, Zhuhai 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.78x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 12:02:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,427
            CDSs (total)                      :: 2,343
            Genes (coding)                    :: 2,279
            CDSs (with protein)               :: 2,279
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 18 of 64
            Pseudo Genes (incomplete)         :: 47 of 64
            Pseudo Genes (internal stop)      :: 18 of 64
            Pseudo Genes (multiple problems)  :: 17 of 64
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142858.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Staphylococcus hominis"
                     /mol_type="genomic DNA"
                     /strain="Sh8213"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1290"
                     /plasmid="pSh8213-3"
                     /geo_loc_name="China: Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2023"
                     /collected_by="Li Chen"
     gene            1..1545
                     /locus_tag="VXJ98_RS12060"
                     /old_locus_tag="VXJ98_12060"
     CDS             1..1545
                     /locus_tag="VXJ98_RS12060"
                     /old_locus_tag="VXJ98_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /protein_id="WP_000622776.1"
                     /translation="MISFFTKTTDMMTSKKRWTALVVLAVSLFVVTMDMTILIMALPEL
                     VRELEPSGTQQLWIVDIYSLVLAGFIIPLSAFADKWGRKKALLTGFALFGLVSLAIFFA
                     ESAEFVIAIRFLLGIAGALIMPTTLSMIRVIFENPKERATALAVWSIASSIGAVFGPII
                     GGALLEQFSWHSAFLINVPFAIIAVVAGLFLLPESKLSKEKSHSWDIPSTILSIAGMIG
                     LVWSIKEFSKEGLADIIPWVVIVLAITMIVIFVKRNLSSSDPMLDVRLFKKRSFSAGTI
                     AAFMTMFAMASVLLLASQWLQVVEELSPFKAGLYLLPMAIGDMVFAPIAPGLAARFGPK
                     IVLPSGIGIAAIGMFIMYFFGHPLSYSTMALALILVGAGMASLAVASALIMLETPTSKA
                     GNAAAVEESMYDLGNVFGVAVLGSLSSMLYRVFLDISSFSSKGIVGDLAHVAEESVVGA
                     VEVAKATGIKQLANEAVTSFNDAFVATALVGGIIMIIISIVVYLLIPKSLDITKQK"
                     /name="quaternary ammonium compound efflux MFS transporter
                     QacA"
     gene            1750..2310
                     /locus_tag="VXJ98_RS12065"
                     /old_locus_tag="VXJ98_12065"
     CDS             1750..2310
                     /locus_tag="VXJ98_RS12065"
                     /old_locus_tag="VXJ98_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001077893.1"
                     /translation="MNRADIFKIFDVELNITIFLGALVATFIYFISYMNNVNELITLCL
                     TLAFPAYLLKISRSFEVILLEEKIKNSKIKICDKDRIEREILVKKNLIDELLFPNRVIK
                     KYTLTILIYSIGIIILLLLCNMLSYGIGENVNFIWRLLACYVTSVTVIIVALIIMIMIF
                     DLIIGLLLNFQIFLKNKKYDDFL"
                     /name="hypothetical protein"
     gene            complement(2630..3319)
                     /locus_tag="VXJ98_RS12070"
                     /old_locus_tag="VXJ98_12070"
     CDS             complement(2630..3319)
                     /locus_tag="VXJ98_RS12070"
                     /old_locus_tag="VXJ98_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002497507.1"
                     /translation="MKSMKKIADELNVTKMTVYNNAKKANVKFQKIDNVNYLSSEDEIV
                     VVNRIKKNQKKSIDAESKINEKNSDNDNNQVKDETIKQLYIQLDIYKEQANRDKEQINT
                     LNRLLENQQVLALESNKKIQKLESQLEEERQLNYSFDTATNNRQNINPKEATFTKEPQN
                     ANQQQKKSQPTEVNHKNIAKEKKDDSINEDELTNEDKFVEETKNDPDDNREVEPPKKGF
                     WSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            3803..4771
                     /locus_tag="VXJ98_RS12075"
                     /old_locus_tag="VXJ98_12075"
     CDS             3803..4771
                     /locus_tag="VXJ98_RS12075"
                     /old_locus_tag="VXJ98_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_002497508.1"
                     /translation="MSNFNIKEIQKEKFYRLPKVFFTNPKYTKLSNDAKITWSILRDRL
                     DLSIKNNWIDENGDIFFIYTNEKLKQILNISSPNKLSKIKKELTRADLFSQIRVGLNKP
                     NKLYLKKPEVTKADVYYITQQENDVEQQYSTDVSNSYIQKYDNDTSGNIKMIHHDISKK
                     YTNDTDYNDTDYIKTENNDMDDLNDKKSTYPSNHTNHSNHDNSNFNNEALKFQLLEELP
                     QSIQNYLSNFEVAEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVSVLKRFKATLIQ
                     KNETVEAMQGYLMKSLKSEFAEVHTLNKRRDHLPITSLFNQ"
                     /name="replication initiator protein A"
     gene            complement(4995..5234)
                     /locus_tag="VXJ98_RS12080"
                     /old_locus_tag="VXJ98_12080"
     CDS             complement(4995..5234)
                     /locus_tag="VXJ98_RS12080"
                     /old_locus_tag="VXJ98_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="WP_002449925.1"
                     /translation="MNDFQNLKKTNAAIEKAELRKHRLKNLDRKERAHRLIRKGAMLEK
                     YFECEHLSPDETEELLKMYSKYINTNKPNKFKKK"
                     /name="DUF3847 domain-containing protein"
     gene            complement(5239..5520)
                     /locus_tag="VXJ98_RS12085"
                     /old_locus_tag="VXJ98_12085"
     CDS             complement(5239..5520)
                     /locus_tag="VXJ98_RS12085"
                     /old_locus_tag="VXJ98_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002449920.1"
                     /translation="MSKIKQTDISKLSQLEQQIEQLKEQKKQQEAQLSNKIGGYFLKKL
                     NLNMINSNDELYKIMDKVIEEFNKDIEQNNSNGNNENLKENEINQNSK"
                     /name="hypothetical protein"
     gene            5724..7646
                     /locus_tag="VXJ98_RS12090"
                     /old_locus_tag="VXJ98_12090"
     CDS             5724..7646
                     /locus_tag="VXJ98_RS12090"
                     /old_locus_tag="VXJ98_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_053015945.1"
                     /translation="MEKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HTVKPESMILTPQNAPEWTKDRQRLWNEVDKVEKHNAKTNNPRLAKEILLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDMNNPHAHVLLTQRPFNSDGTWGKKTKTRTQ
                     YDENGNAILNKNGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNEKKRCEKEGIEYQPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANNQVSENEQSYSILFDRYKHDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRSHLKFYADNYDTNYQFRREVENKGYSQQEFINTVKSNFKELLNEK
                     NKLESREEKRKEIYQSAKDVYLTEIDKNNLIVQQMYPKSHNQFSNDEKAFIVDEAQKGN
                     IIKASNVQYLFKQNNYIPSNVFLEDTYKKASKDIFFSERQLKNLEKDSFEYNVELQFIK
                     EKEKEIDSYKEHIDDDLKIDIGNRNFEKVKDQTPNEKVQLLIKLEKYNNINQEEVIEKH
                     IHQVDRNDYDNHLEHEDGQSNHINLPTINASDFLMYYANETEKQNNINHKKKKKKQDRN
                     NQMRR"
                     /name="MobA/MobL family protein"
     gene            7888..8010
                     /locus_tag="VXJ98_RS12095"
                     /old_locus_tag="VXJ98_12095"
     CDS             7888..8010
                     /locus_tag="VXJ98_RS12095"
                     /old_locus_tag="VXJ98_12095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_281340014.1"
                     /translation="MQDEFSKQFVTGRRIKMKCWECGFSTLKIVSKKLSRHGKL"
                     /name="hypothetical protein"
     gene            8339..8440
                     /locus_tag="VXJ98_RS12100"
                     /old_locus_tag="VXJ98_12100"
     CDS             8339..8440
                     /locus_tag="VXJ98_RS12100"
                     /old_locus_tag="VXJ98_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_016931398.1"
                     /translation="MLDNFVHIMTTAASGCIVALFAHWLRNRNDNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(8613..9584)
                     /locus_tag="VXJ98_RS12105"
                     /old_locus_tag="VXJ98_12105"
     CDS             complement(8613..9584)
                     /locus_tag="VXJ98_RS12105"
                     /old_locus_tag="VXJ98_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_053015944.1"
                     /translation="MFNAVVVRKSASNVSYKVETVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFSGIVESSSNDKFKEGDNVLVTGYDVGMTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILRKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            9708..10127
                     /locus_tag="VXJ98_RS12110"
                     /old_locus_tag="VXJ98_12110"
     CDS             9708..10127
                     /locus_tag="VXJ98_RS12110"
                     /old_locus_tag="VXJ98_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418034.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINETTF"
                     /name="MarR family transcriptional regulator"
     gene            10398..11006
                     /locus_tag="VXJ98_RS12115"
                     /old_locus_tag="VXJ98_12115"
     CDS             10398..11006
                     /locus_tag="VXJ98_RS12115"
                     /old_locus_tag="VXJ98_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583537.1"
                     /translation="MIIGYARVSSVDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIVQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKNGKGLS"
                     /name="recombinase family protein"
     gene            complement(11209..11775)
                     /gene="qacR"
                     /locus_tag="VXJ98_RS12120"
                     /old_locus_tag="VXJ98_12120"
     ARG             complement(11213..11775)
                     /name="qacR"
                     /gene="qacR"
                     /gene_name="multidrug-binding transcriptional regulator
                     QacR"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.47"
                     /coverage="100.0"
     ARG             2..1542
                     /name="qacA"
                     /gene="qacA"
                     /gene_name="quaternary ammonium compound efflux MFS
                     transporter QacA"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3804..4771
                     /name="rep_cluster_1110"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5725..7577
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.424"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atgatttcat tttttacaaa aactactgat atgatgacat caaaaaaaag atggactgca
       61 ctagtagtat tagctgttag tttgtttgtt gttacaatgg atatgacaat attaattatg
      121 gctttaccgg aattagtaag agagttagag ccttctggta cccaacagtt atggatagtt
      181 gatatatact ctcttgtttt agctggcttt ataattccat tgagtgcctt tgctgataaa
      241 tggggaagaa aaaaagcatt attaactgga tttgctttat ttggcctcgt ttcattagct
      301 atatttttcg cagaaagtgc agagttcgta atagctattc gatttttact tggtattgca
      361 ggtgctttaa taatgccaac tactctttca atgataagag taatttttga aaaccctaaa
      421 gaaagggcca ctgcattagc tgtatggtca atcgcttcat cgataggtgc tgtttttgga
      481 ccaattatcg gaggagcttt acttgagcaa ttttcatggc actcggcatt tttaattaat
      541 gtaccgtttg cgataatagc agttgtagca ggtttatttt tattaccaga gtctaagtta
      601 tcaaaagaaa agtctcactc gtgggatatt ccttctacaa ttttatcaat tgcaggcatg
      661 attggactgg tatggagtat caaagaattt tcaaaagaag gactagcaga tattattcca
      721 tgggttgtaa tagtattagc aattaccatg atagtgatat ttgttaaacg taatttatca
      781 agttctgatc caatgttaga cgtaagactt tttaaaaaga gatcattttc agctggtaca
      841 attgctgcat ttatgacaat gtttgcaatg gcatctgttt tgttattagc ttcacaatgg
      901 ttacaggttg tggaagaact ttctcctttt aaagctggct tatacctatt acctatggca
      961 ataggagata tggtgtttgc accaattgca cccggattag cggcgcgatt tggaccgaaa
     1021 atagtgttac cttccggaat tggaattgca gccattggca tgtttattat gtatttcttt
     1081 ggtcatccat tatcatattc tacaatggct ttagcattaa ttttagttgg agctggtatg
     1141 gcttcactag cagttgcatc tgctctaata atgttagaaa cacctacatc aaaagcaggt
     1201 aatgcagctg ctgttgaaga gtctatgtat gaccttggaa atgtttttgg tgtagcagta
     1261 cttggtagcc tatcttctat gctttatcgt gtatttttag atatttcatc tttttcatca
     1321 aaaggtatag ttggagattt agctcatgta gctgaagaat ctgtagtggg cgctgtcgaa
     1381 gtagctaaag ctacggggat aaaacagctt gcaaacgagg ctgtaacatc atttaatgat
     1441 gcttttgtag caactgcttt agtaggtggg attatcatga ttatcatttc aatagttgtc
     1501 tatttgttaa ttcccaaatc acttgatata actaaacaaa aatagtacta aacatatatt
     1561 tttagatatt gtagattagt ttatttttaa aattagtaat agttaaaaag tagaaccatt
     1621 aattttaaat ggttctactt ttttattatt aagtaaaatt ataaaagaca atttcttaaa
     1681 ttaataatat gatataccta tatctaataa tttattagta ttaataaacc ttgaaaggat
     1741 gattaactga tgaatagagc agatattttt aaaatatttg atgtagaact taatataact
     1801 atatttttag gagcactagt agcaactttc atctatttta taagttacat gaacaatgta
     1861 aatgaattaa ttacattatg cttaacatta gcttttcctg catatttatt aaaaattagc
     1921 agatcgttcg aggttatttt attagaggaa aaaataaaaa atagtaaaat caaaatctgt
     1981 gacaaagata gaattgaacg agagatactg gtgaaaaaga acctaataga tgaactattg
     2041 tttcctaata gagttattaa aaaatataca ctgacaattt taatttacag tataggtatt
     2101 attattcttt tactactttg taatatgtta agttatggaa ttggtgaaaa tgtcaatttt
     2161 atctggagat tactagcttg ttatgtaacg agtgtcacag tgattatagt tgccttgatt
     2221 ataatgataa tgatatttga tcttataatt ggattactat taaattttca aatattttta
     2281 aaaaataaaa agtatgatga ctttttataa ttattcgatt tatataatga aaaattaaat
     2341 gattaaacat agaaacctca aaacaaacca tatttaatag agtttatggc gccttataca
     2401 aataaaggct atgctgttaa agaattatgt agctatcttc agattaatat ggatgatgtt
     2461 tatactattg gcgatagaaa attgatttac ctatgtttaa taccctagat aattcttctg
     2521 ataatttagt ttttgttaga gtttaacata actgatgatt atcagaagtt tgagtcacat
     2581 taaaaaggca caacaagttc atgaacttgt tgtgcctttt taattgcaat tagttaccaa
     2641 ataaacgact ccaaaatccc tttttaggtg gttctacctc cctattatca tcaggatcat
     2701 tcttcgtttc ttcaacgaac ttatcctcat ttgttagttc atcttcgttt atactgtcat
     2761 cctttttttc tttagctata ttcttatgat ttacttcggt tggttggctt ttcttttgtt
     2821 gttggttcgc attttgaggt tctttagtaa aagttgcttc tttcggatta atattttgtc
     2881 tattattcgt cgctgtatca aaagaataat taagctgtct ttcttcttct aattggcttt
     2941 ctagtttttg aattttttta ttgctttcta atgctaagac ttgctgattt tctaaaagtc
     3001 tattaagtgt atttatttgt tctttatctc tatttgcctg ttccttatat atatctagct
     3061 gaatatataa ctgctttatt gtttcatctt ttacctgatt attatcgtta tcagaatttt
     3121 tttcatttat tttactttca gcatcaatgc tctttttctg atttttttta attctattta
     3181 ctactacaat ctcatcttca ctagataagt aatttacgtt atcaattttt tgaaatttta
     3241 catttgcttt tttagcatta ttataaactg tcatttttgt cacatttagt tcgtcagcga
     3301 ttttcttcat acttttcata tagtagcctc cgtataacta tatatatatg atatttatta
     3361 tgattatata gttattatac ttaccatatc aatactttta tacttataca taaaatatat
     3421 acatataatt atatgtatat atttcaccct ctttatacgc ttcttatata ccttttatac
     3481 gtattgcaat agctttcttt atataacatt ccatatcact atataaaaac aaaatataac
     3541 cttttatcat tcttacatga tatatataca ctaaatatat ataaaaaatg taacaaagat
     3601 tgggaaaatg ttattataaa tagaaagaag cctaactacg ggaatagtta ggcctcgatg
     3661 gtaataaaaa agttgttttt gtacagttgt tgtattcata ttatatatat taagaattat
     3721 tagcgcaagt ctaatacata tatacttact tatgactttt tattacctct tttaaaattt
     3781 aaatatgaaa gaggtttttt gtatgtctaa ttttaatatt aaagaaatcc aaaaagaaaa
     3841 attctatcga ttgcctaaag tattttttac aaatcctaaa tatactaaat tgtccaatga
     3901 tgcaaaaata acttggtcaa tattaagaga tcgtttagat ttatctataa aaaataattg
     3961 gatagatgaa aatggagata tattctttat atatactaac gaaaagttaa aacaaatcct
     4021 aaatattagt agcccaaaca aattatctaa aataaagaag gaattaactc gagctgattt
     4081 gtttagtcaa atcagagtag gattaaataa acccaataaa ctgtatctaa aaaaacctga
     4141 agtaacaaaa gctgatgtat attacattac acaacaagaa aatgacgtag aacagcaata
     4201 ttctacggat gtatcaaatt catacatcca gaaatatgat aatgatacat caggaaatat
     4261 taaaatgata catcatgata tatcaaaaaa atacactaat gatactgatt ataatgatac
     4321 tgattatatt aagactgaga ataatgatat ggatgatttg aatgataaga aatcaacata
     4381 tcctagtaat catacaaatc attcaaatca cgacaattca aactttaata atgaggcttt
     4441 aaaattccaa ttactcgaag aactaccaca gagtattcaa aactatctaa gtaactttga
     4501 agtagctgaa attaaactta ttaaatctgt gttattaaaa gccaaaacat ccttcaacaa
     4561 ttctattgat gcatattatt tattagaaga tatggaattt gaaattgtta gtgttcttaa
     4621 gcgttttaaa gctacattaa ttcaaaaaaa tgaaaccgtt gaagcaatgc aagggtactt
     4681 aatgaaatct cttaaatctg aatttgcaga agtacatacg cttaataaac gacgtgatca
     4741 tttaccaatc acttctttat ttaatcaata atacaactta ataagccaac aatgatccaa
     4801 ttagagaata tctttaaata gatattctct taactgtagg cgaactttat tttcaatgaa
     4861 atgtaattaa ttaagtaagt gggactaaaa ttgaaacgtt tagtcccatt tttataaaca
     4921 attaatattc attgttacta ctatcagcaa ttttttcttt ttagtggaat ccaagtattt
     4981 gtaagcatga taaattactt ctttttaaat ttatttggct tattcgtatt aatgtatttg
     5041 gaatacatct ttaacaattc ttctgtttca tctggactta aatgttcaca ttcaaaatat
     5101 ttttcaagca ttgcaccttt tcgaataagt ctatgcgctc tttctttgcg atctaaattt
     5161 tttaatctat gttttcttaa ttcagctttt tcaattgcag catttgtttt tttgagattt
     5221 tgaaaatcat tcatcttatc acttactatt ctgattaatt tcgttttctt ttaaattttc
     5281 attgttcccg ttgctgttat tttgttcgat atctttatta aattcttcaa tgactttatc
     5341 cataatttta tacaattcat cattagagtt aatcatattt aaattaagtt tttttaaaaa
     5401 ataaccacca attttattag atagttgtgc ttcttgttgt ttcttttgtt ctttcagttg
     5461 ttcaatttgt tgttctaatt gacttaactt tgaaatatcg gtttgtttga ttttagacat
     5521 taaaaaactc ccttagttta tctatataaa gcataccata attcattggg taagtcgtac
     5581 attcatggta taatgtaacc agttcttaag acactgccca tttacatgca aattagaaat
     5641 tggcataaaa atgggcaacc atactggttg atcgttatag ttcccacagg ggtttaaatt
     5701 caaaaaagcg aggaggtaaa atcatggaaa aaggttactt tatgttgaga gtgactaaca
     5761 ttaatcgagc aacaaaaaat attgttgcat cagcgtcgta tcgttctgat gaagcattat
     5821 atagtgaacg tacagatgaa aaaattaagt ttagaaatca tactgttaaa cctgaaagca
     5881 tgattttaac gcctcaaaat gcacctgaat ggactaaaga tagacagcgt ttgtggaatg
     5941 aagttgataa agttgaaaag cataatgcga aaacaaataa ccctagatta gcaaaagaaa
     6001 tcttgttaag tttacctaat gattttgata gagaagtaca aactgaatta acaaaagatt
     6061 ttataaaaag tgagtttgta gacaaaggaa tggttgctga tatttcgatt catagagatg
     6121 atatgaacaa cccacacgcc catgttttac ttactcaaag accttttaat tcagatggta
     6181 cttgggggaa aaagacaaaa acgagaacgc aatatgatga aaatggcaat gcgattttaa
     6241 ataaaaatgg taataaagtt agaaaacaag aacgctttgc cgactttgat ttcaaagaag
     6301 ttcgtaagca ttgggaatta aaattaaatc aatactcaga acgtgaacaa agtttaagac
     6361 gatatgatag tcgttctttt gaagcgcaag gtttagaaaa aatcgcagaa attccactta
     6421 caagagaaga atatagatta gaacaaaacg aaaaaaaacg ctgtgaaaaa gagggtatcg
     6481 agtaccaacc tgttacttat tatggacaga aaaatgaaca aattagaaga tataatcgtg
     6541 gcgaagtatc acaattattt agtgataaag agaaagaaca agcacaaaat gaaattagat
     6601 tatttattca aaatgctaac aatcaagtaa gcgaaaatga acaaagttat tctattttat
     6661 ttgatagata taaacatgat gttggctatt cagaggcaaa agaaactatt aaaaacacgt
     6721 ttgatagtgc gtctactttt ggtcgtaaaa ttcaaaatga cttactcaaa aatgaaatta
     6781 agagaagtca cttaaaattt tatgcagata attatgatac taattatcaa tttagaagag
     6841 aagttgaaaa taaaggatat agtcaacaag agtttattaa tactgttaaa tctaacttta
     6901 aagaattact caatgaaaag aataagttag aaagcagaga agaaaaacgt aaagaaattt
     6961 atcaatcagc taaagacgtt taccttactg aaattgataa aaacaaccta attgttcagc
     7021 aaatgtaccc taaatctcat aatcaattta gtaatgatga gaaagcattt attgttgatg
     7081 aggcacaaaa aggtaatatt ataaaagcat ctaatgttca atatctattc aaacaaaaca
     7141 actatatacc ttcgaatgta tttctagaag atacctataa aaaagcgagt aaggatattt
     7201 tctttagtga aagacagctt aaaaacttag aaaaagatag ctttgaatat aatgttgagt
     7261 tgcaatttat taaggaaaaa gaaaaagaaa ttgatagtta taaagaacat attgatgatg
     7321 atttaaaaat agatataggc aatagaaatt ttgaaaaagt gaaagaccaa acacctaatg
     7381 agaaagttca attactcatt aagcttgaga aatataataa tatcaatcaa gaagaagtta
     7441 tcgaaaaaca tattcatcaa gtggatagaa atgattatga caatcatctt gaacatgaag
     7501 atggacaatc taatcatatt aatttaccaa cgataaatgc tagtgacttc cttatgtatt
     7561 atgcgaatga aacagaaaaa caaaacaata ttaatcataa gaaaaagaag aagaaacaag
     7621 atagaaataa ccaaatgaga agatagtagg ccgttgaaat gatacaaagt tcattcttta
     7681 ataagaaact taaacaaatg tgatacattg actttattta aaaaataaca tttgaaattc
     7741 attattatat atggaatttt aacttatcaa attgattgaa acaaatgtcg gcatagcgtg
     7801 agctattaag cagactattc gaaaagtttt gatattgttg aaaatccaag tttcaatatc
     7861 aataaagcaa aaaggagaat tgactttatg caagatgaat tttcaaagca atttgtgact
     7921 ggtcgaagaa taaaaatgaa gtgttgggaa tgtggttttt caacattgaa aattgtaagt
     7981 aaaaaattgt caagacatgg aaaattataa agcttgttca atttttacga agtgtgaaaa
     8041 ttgtaatgct catgacgatt tttttataaa taaagattat tacggatata ttgatgcaga
     8101 tgttaagccg ttaagtgggg aatggaagtt ataaaattcc ttcgcgtgcg tggaaaaatt
     8161 ggatttgaat ttttggtttt tgaaacgagt tgaaaacgag tttcttcttg tcttgatagt
     8221 tatttagtca taaaatgact tatcggaagt taactattat cgcatattaa gagatgatat
     8281 aatatacagg tcgcctatct ctcaggcgtc aattttgacg cagggaggag gtgtaattat
     8341 gcttgataac tttgttcaca tcatgaccac agcagcgagt ggttgtatcg tcgcattatt
     8401 tgcgcactgg ctacgcaatc gcaacgataa taaaaaatag gcgactatag ccacacctaa
     8461 aaaaatcccc tcactacttc cgatagtgag gggattggtg taatatactt aataactttg
     8521 ttaagtatat tataacactg gttagcggag taatgcaaaa tgattgaatt agtagtaaat
     8581 gtattaatga atactcatac tcatacttat acttatactt ttaaaatagt tcttcctaaa
     8641 tgttgtcctt ttaaaagctg atctatcgtt ttatagaatt cttcaaatcc aatttcattg
     8701 tatagaagtg aatttcctat attccattca tttgctaact tactccatat atcttcacgg
     8761 atattatgat caacatatac agaatcaatg ccaattaatt taatacctct aagaataaac
     8821 ggtaatatat tagcgttaag tgaatttcct gctacatttc cacatacagt agcaacacct
     8881 tgataattta gacgttttaa accgtataat gttacattac tccccactgt atctaagaaa
     8941 taatcaaaaa tacctttttt aagcggttta ttatcatctt caattattat tacttgtttt
     9001 gcacctaatt tttttgcgat ttctatttgc ttttcttttc ttacgatagc agtgatattt
     9061 tcatatccaa ttttacgtaa aatttgtata gcaacactgc ccacaccacc tgtagcccca
     9121 gacactaaaa tttcaggttg tgatttgata ttcattccat atttttctag ttcaaaaatt
     9181 gataatgctg ccgtaaaacc ggctgtgcca tataccatag actctttaat agacatttct
     9241 tggggaatag gaacaatcca ctctccaggc acttgtgcat attctgaaaa tccacctgta
     9301 tgtgtcatgc caacatcata accagtaact aatacattat cgccttcttt aaacttatca
     9361 ttcgaacttg attcaacaat acctgaaaaa tctataccag gaatcatagg ataatctcta
     9421 attacaccgc ctttatattg taaagctagc atatccttat aatttattga agagtatacc
     9481 acttttacta agacatcacc cttcgataaa tgagattcat caacggtttc tactttataa
     9541 ctaacatttg atgcactttt tctcactact accgcattga acatttatta gccttctttc
     9601 acaattgttt tgtagaagta tgtttttgtt gcaaaactat ttccctacaa aacttatcat
     9661 gatatgataa gcgagtcaat actaatgatt taagagaggt tttagttgtg gaattagata
     9721 attgtataaa ttatttgttg agtacttctc agaatagagt gtttaaacat tttaaaagtt
     9781 tattaagccc ctataatatt actccagcag aatatggtgt tttgaattgc ttgattaata
     9841 gtaatttgaa tacacctaaa gcgattggta atactttaaa tttagaacct tcaacaatat
     9901 ctggaatatt agataagatg tccaaaaagg atctaataac acgtcaaact gataatgaaa
     9961 atagaagaac tgtgctaata aacagtacaa ataaagcaaa agacttatgg ccaacattag
    10021 aaaagaaagc ctatcaactt aataatcatt acttcgctaa tttaactaat gaagaaactg
    10081 tagtgtttaa aaagatatta ctaaagataa atgaaactac gttttaattt ttacgttatt
    10141 aaaaagttga agtgcgaatt ataatcgagt aaatataaat gatagcttag cttaattata
    10201 tattaaagcc aactatactt ttattcccga aaaacctact atccaaattt ctaagatact
    10261 ttagcttcta taataaacat attgtaatat aatttatgta ttatttaggt acaccctaat
    10321 catacaaaga gagttattga atttataata acttaaacgt atgattaggg tgtatattaa
    10381 ttgtggagtg aaaatgaatg attataggtt atgcaagagt atcttcggta gatcagaatt
    10441 tagagagaca gctagataat ttgaaaacat ttggcgtaga gaaaatattt acagagaaac
    10501 aatcgggaaa gtcagtagaa aatagacccg tatttcaaga agcacttaac tttgtgagaa
    10561 tgggcgatag atttgtagta gaatcgattg atcgcttagg tcgtaattat gatgaagtga
    10621 ttaacacagt taattattta aaggataaag aagttcaatt gatgattact agtttaccta
    10681 tgatgaatga agtgattggt aatccactat tagataaatt tatgaaagat ttaatcgttc
    10741 aaatcttagc aatggtttca gaacaagaac gaaatgaaag taaacgtaga caagcacaag
    10801 gtattcaagt tgcgaaagaa aaaggtgtgt ataaaggacg ccctttacta tattcaccta
    10861 atgctaaaga tcctcaaaaa cgtattatct accatcgtgt tgttgaaatg ttagaagaag
    10921 gcaaagcaat tagtaagatt gcgaaagagg ttaatatcac aagacagaca gtttatagaa
    10981 ttaaaaatgg taagggatta tcttgataaa tggattgtta atgcaaatga gatcttctca
    11041 cagcgtccta aattatatgt tattcatcta ttatgagatt ctaataataa gacaagaaaa
    11101 gagagtgcga attcgcactc tccttttgtg tagtactatt gaaattttgt cttaaaaacg
    11161 aacgtaattt agaaataata tttattggta tttcaagaat atatataatt atttactaag
    11221 tccatttaaa aaaatttgag agaacttgtt cataagttta attctttcat taatattttg
    11281 ttcatgtgta aaggtaacaa ttccattaac agcgtttgct gctattttac taacagcatt
    11341 aacatcatta atacaccatt cgccatttaa attaccctcc ttaaaaatta catgataagc
    11401 atctatatat ttgttttcta atttattcat tttttcatta atgctattag ttttataata
    11461 ttcagtgcaa aattcaatta ttgcgttttg aagtggataa tagtattcgg ttgttaaaga
    11521 tagttcatta tataaataaa atttttctct attagtttta catttgattt gttccttttt
    11581 ccactgttct tgccatttag attcttctat atttaaaatt tctaaaaata gattttcttt
    11641 tgttttaaag tgataataaa gattcccttt actactttct gataatttaa caatttctcc
    11701 agtagtagtg gcattatatc cattttttat aaataattcc tttgcgacac ctagtatttt
    11761 atctttcaag ttcatcacgc ctttaatgag tattaagtac tatcaatgat agcacaaaaa
    11821 attcaaagaa taattgtata acacaataca gccatttaaa tttcgcaaga ttttttgttg
    11881 taatatgtaa aaaaatagat tacaatcctt atagaccgat cgcacggtct ataaggattg
    11941 gagggaactt aa
//
